Cell-extracellular matrix dynamics

Physical Biology ◽

10.1088/1478-3975/ac4390 ◽

2021 ◽

Author(s):

Andrew Doyle ◽

Shayan Nazari ◽

Kenneth M Yamada

Keyword(s):

Extracellular Matrix ◽

Cell Migration ◽

Cell Types ◽

Tissue Level ◽

Matrix Proteins ◽

Immune Functions ◽

Integrin Receptors ◽

Invasion And Metastasis ◽

Disease States ◽

A Cell

Abstract The sites of interaction between a cell and its surrounding microenvironment serve as dynamic signaling hubs that regulate cellular adaptations during developmental processes, immune functions, wound healing, cell migration, cancer invasion and metastasis, as well as in many other disease states. For most cell types, these interactions are established by integrin receptors binding directly to extracellular matrix proteins, such as the numerous collagens or fibronectin. For the cell, these points of contact provide vital cues by sampling environmental conditions, both chemical and physical. The overall regulation of this dynamic interaction involves both extracellular and intracellular components and can be highly variable. In this review, we highlight recent advances and hypotheses about the mechanisms and regulation of cell-ECM interactions, from the molecular to the tissue level, with a particular focus on cell migration. We then explore how cancer cell invasion and metastasis are deeply rooted in altered regulation of this vital interaction.

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Formation and anatomy of the prestalk zone of Dictyostelium

Development ◽

10.1242/dev.107.supplement.91 ◽

1989 ◽

Vol 107 (Supplement) ◽

pp. 91-97 ◽

Cited By ~ 2

Author(s):

J. G. Williams ◽

K. A. Jermyn ◽

K. T. Duffy

Keyword(s):

Extracellular Matrix ◽

Cell Migration ◽

Cell Types ◽

Matrix Proteins ◽

Anterior Region ◽

Stalk Cell ◽

Directed Cell Migration ◽

Pattern Forming ◽

Different Parts ◽

Primary Pattern

The pDd63 and pDd56 genes encode extracellular matrix proteins which, respectively, surround the migratory slug and mature stalk cells. Both genes are dependent for their expression upon, and rapidly induced by, DIF, the stalk cell inducer. Using these genes as cell-autonomous markers, we have defined three distinct kinds of ‘prestalk’ cells localized to different parts of the anterior region of the slug. At least one, and probably both, prestalk cell types initially differentiates at the base of the aggregate. The most abundant of the two prestalk cell types then migrates into the tip, the precursor of the prestalk zone which arises at the apex of the aggregate. Thus we believe that morphogenesis of the prestalk zone, the primary pattern-forming event in Dictyostelium development, involves a combination of positionally localized differentiation and directed cell migration. To account for the positionally localized differentiation of prestalk cells, we invoke the existence of gradients of the known antagonists of DIF — cAMP and NH3. We further suggest that differences in the motility of pstA and pstB cells might result from differences in their chemotactic responsiveness to cAMP signals propagated from the tip.

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Mitochondria tether to Focal Adhesions during cell migration and regulate their size

10.1101/827998 ◽

2019 ◽

Author(s):

Redaet Daniel ◽

Abebech Mengeta ◽

Patricia Bilodeau ◽

Jonathan M Lee

Keyword(s):

Extracellular Matrix ◽

Cell Migration ◽

Energy Production ◽

Focal Adhesions ◽

Leading Edge ◽

Energy Requirements ◽

Integrin Receptors ◽

Cellular Energy ◽

A Cell ◽

Atp Generation

AbstractMitochondria are the key generators of ATP in a cell. Visually, they are highly dynamic organelles that undergo cellular fission and fusion events in response to changing cellular energy requirements. Mitochondria are now emerging as regulators of mammalian cell motility. Here we show that mitochondria infiltrate the leading edge of NIH3T3 fibroblasts during migration. At the leading edge, we find that mitochondria move to and tether to Focal Adhesions (FA). FA regulate cell migration by coupling the cytoskeleton to the Extracellular Matrix through integrin receptors. Importantly, we find that inhibition of mitochondrial ATP generation concomitantly inhibits FA size. This suggests that mitochondrial energy production regulates migration through FA control.

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Faculty Opinions recommendation of The neural cell adhesion molecule L1 potentiates integrin-dependent cell migration to extracellular matrix proteins.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1009197.119458 ◽

2002 ◽

Author(s):

Genevieve Rougon

Keyword(s):

Extracellular Matrix ◽

Cell Migration ◽

Cell Adhesion ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Neural Cell Adhesion Molecule ◽

Matrix Proteins ◽

Neural Cell Adhesion ◽

Dependent Cell ◽

Cell Adhesion Molecule L1

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Analyses of cellular multimerin 1 receptors: in vitro evidence of binding mediated by αΙΙbβ3 and αvβ3

Thrombosis and Haemostasis ◽

10.1160/th05-02-0140 ◽

2005 ◽

Vol 94 (11) ◽

pp. 1004-1011 ◽

Cited By ~ 31

Author(s):

Frédéric Adam ◽

Shilun Zheng ◽

Nilesh Joshi ◽

David Kelton ◽

Amin Sandhu ◽

...

Keyword(s):

Extracellular Matrix ◽

Cell Types ◽

Factor V ◽

Cellular Activation ◽

Fiber Assembly ◽

Expression Studies ◽

Additional Cell ◽

A Cell ◽

Rgd Site

SummaryMultimerin 1 (MMRN1) is a large, soluble, polymeric, factor V binding protein and member of the EMILIN protein family.In vivo, MMRN1 is found in platelets, megakaryocytes, endothelium and extracellular matrix fibers, but not in plasma. To address the mechanism of MMRN1 binding to activated platelets and endothelial cells, we investigated the identity of the major MMRN1 receptors on these cells using wild-type and RGE-forms of recombinant MMRN1. Ligand capture, cell adhesion, ELISA and flow cytometry analyses of platelet-MMRN1 binding, indicated that MMRN1 binds to integrins αIIbβ3 and αvβ3. Endothelial cell binding to MMRN1 was predominantly mediated by αvβ3 and did not require the MMRN1 RGD site or cellular activation. Like many other αvβ3 ligands, MMRN1 had the ability to support adhesion of additional cell types, including stimulated neutrophils. Expression studies, using a cell line capable of endothelial-like MMRN1 processing, indicated that MMRN1 adhesion to cellular receptors enhanced its extracellular matrix fiber assembly. These studies implicate integrin-mediated binding in MMRN1 attachment to cells and indicate that MMRN1 is a ligand for αIIbβ3 and αvβ3.

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Inducible cell-specific mouse models for paired epigenetic and transcriptomic studies of microglia and astroglia

Communications Biology ◽

10.1038/s42003-020-01418-x ◽

2020 ◽

Vol 3 (1) ◽

Cited By ~ 1

Author(s):

Ana J. Chucair-Elliott ◽

Sarah R. Ocañas ◽

David R. Stanford ◽

Victor A. Ansere ◽

Kyla B. Buettner ◽

...

Keyword(s):

Gene Expression ◽

Affinity Purification ◽

Regulation Of Gene Expression ◽

Cell Types ◽

Tissue Level ◽

Cell Phenotype ◽

Specific Gene ◽

Cell Type ◽

A Cell ◽

Cell Type Specific

AbstractEpigenetic regulation of gene expression occurs in a cell type-specific manner. Current cell-type specific neuroepigenetic studies rely on cell sorting methods that can alter cell phenotype and introduce potential confounds. Here we demonstrate and validate a Nuclear Tagging and Translating Ribosome Affinity Purification (NuTRAP) approach for temporally controlled labeling and isolation of ribosomes and nuclei, and thus RNA and DNA, from specific central nervous system cell types. Analysis of gene expression and DNA modifications in astrocytes or microglia from the same animal demonstrates differential usage of DNA methylation and hydroxymethylation in CpG and non-CpG contexts that corresponds to cell type-specific gene expression. Application of this approach in LPS treated mice uncovers microglia-specific transcriptome and epigenome changes in inflammatory pathways that cannot be detected with tissue-level analysis. The NuTRAP model and the validation approaches presented can be applied to any brain cell type for which a cell type-specific cre is available.

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