Formation and anatomy of the prestalk zone of Dictyostelium

Development ◽  
1989 ◽  
Vol 107 (Supplement) ◽  
pp. 91-97 ◽  
Author(s):  
J. G. Williams ◽  
K. A. Jermyn ◽  
K. T. Duffy
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Cell Types ◽  
Matrix Proteins ◽  
Anterior Region ◽  
Stalk Cell ◽  
Directed Cell Migration ◽  
Pattern Forming ◽  
Different Parts ◽  
Primary Pattern

The pDd63 and pDd56 genes encode extracellular matrix proteins which, respectively, surround the migratory slug and mature stalk cells. Both genes are dependent for their expression upon, and rapidly induced by, DIF, the stalk cell inducer. Using these genes as cell-autonomous markers, we have defined three distinct kinds of ‘prestalk’ cells localized to different parts of the anterior region of the slug. At least one, and probably both, prestalk cell types initially differentiates at the base of the aggregate. The most abundant of the two prestalk cell types then migrates into the tip, the precursor of the prestalk zone which arises at the apex of the aggregate. Thus we believe that morphogenesis of the prestalk zone, the primary pattern-forming event in Dictyostelium development, involves a combination of positionally localized differentiation and directed cell migration. To account for the positionally localized differentiation of prestalk cells, we invoke the existence of gradients of the known antagonists of DIF — cAMP and NH3. We further suggest that differences in the motility of pstA and pstB cells might result from differences in their chemotactic responsiveness to cAMP signals propagated from the tip.

Cell-extracellular matrix dynamics

2021 ◽  
Author(s):  
Andrew Doyle ◽  
Shayan Nazari ◽  
Kenneth M Yamada
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Cell Types ◽  
Tissue Level ◽  
Matrix Proteins ◽  
Immune Functions ◽  
Integrin Receptors ◽  
Invasion And Metastasis ◽  
Disease States ◽  
A Cell

Abstract The sites of interaction between a cell and its surrounding microenvironment serve as dynamic signaling hubs that regulate cellular adaptations during developmental processes, immune functions, wound healing, cell migration, cancer invasion and metastasis, as well as in many other disease states. For most cell types, these interactions are established by integrin receptors binding directly to extracellular matrix proteins, such as the numerous collagens or fibronectin. For the cell, these points of contact provide vital cues by sampling environmental conditions, both chemical and physical. The overall regulation of this dynamic interaction involves both extracellular and intracellular components and can be highly variable. In this review, we highlight recent advances and hypotheses about the mechanisms and regulation of cell-ECM interactions, from the molecular to the tissue level, with a particular focus on cell migration. We then explore how cancer cell invasion and metastasis are deeply rooted in altered regulation of this vital interaction.

Role of high molecular weight extracellular matrix proteins in glioma cell migration

1997 ◽  
Vol 23 (2) ◽  
pp. 102-112 ◽  
Author(s):  
R. Mahesparan ◽  
B. B. Tysnes ◽  
K. Edvardsen ◽  
H. K. Haugeland ◽  
I. Garcia Cabrera ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Extracellular Matrix ◽  
Cell Migration ◽  
High Molecular Weight ◽  
Glioma Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Glioma Cell Migration

Promotion of Tumor Cell Migration by Extracellular Matrix Proteins in Human Pancreatic Cancer

Pancreas ◽  
2009 ◽  
Vol 38 (7) ◽  
pp. 804-810 ◽  
Author(s):  
Eduard Ryschich ◽  
Akmal Khamidjanov ◽  
Vachtang Kerkadze ◽  
Markus W. Büchler ◽  
Margot Zöller ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Extracellular Matrix ◽  
Cell Migration ◽  
Tumor Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Human Pancreatic Cancer ◽  
Tumor Cell Migration

Extracellular matrix proteins in intrathymic T-cell migration and differentiation?

1993 ◽  
Vol 14 (4) ◽  
pp. 158-161 ◽  
Author(s):  
Wilson Savino ◽  
Dea Maria S. Villa-Verde ◽  
Joseli Lannes-Vieira
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
T Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
T Cell Migration

Assembly of exogenous fibronectin into type II cell extracellular matrix

1997 ◽  
Vol 272 (5) ◽  
pp. L908-L915 ◽  
Author(s):  
J. W. Swisher ◽  
D. E. Rannels
Keyword(s):  
Extracellular Matrix ◽  
Calf Serum ◽  
Cell Types ◽  
Biologically Active ◽  
Matrix Proteins ◽  
Type Ii ◽  
Attachment Protein ◽  
Pulmonary Epithelial Cells ◽  
Type Ii Cell ◽  
Exogenous Origin

Type II pulmonary epithelial cells (T2P) in primary culture assemble a biologically active extracellular matrix (ECM) from endogenously synthesized components, including fibronectin. Fibronectin is a well-recognized attachment protein that mediates cell adhesion, migration, and cytodifferentiation. In some cell types, exogenous fibronectin also is incorporated into ECM. The latter pathway of ECM assembly was thus investigated in T2P. Cells were cultured for 3-days in Dulbecco's modified Eagle's medium (DMEM) with or without 10% fetal calf serum (FCS), a source of exogenous fibronectin. Cell and matrix fractions were harvested on culture days 1, 2, and 3 to determine synthesis of cell and matrix proteins and matrix fibronectin content. During 3 days in DMEM containing 10% FCS, T2P flattened and spread to confluence more rapidly than cells in DMEM; they also produced ECM with higher fibronectin content than did cells in DMEM alone. On culture days 2 and 3, 10% FCS doubled (on average) synthesis of ECM fibronectin; in contrast, ECM fibronectin content increased nearly 10-fold. These observations suggest that cultured type II cells incorporate exogenous fibronectin into newly assembled ECM to a greater extent than the newly synthesized glycoprotein. Components of both endogenous and exogenous origin may therefore contribute to T2P assembly of a biologically active ECM.

scholarly journals The transcriptional landscape of a hepatoma cell line grown on scaffolds of extracellular matrix proteins

2020 ◽  
Author(s):  
Souvik Ghosh ◽  
Anastasiya Börsch ◽  
Mihaela Zavolan
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Hepatoma Cell ◽  
Cell Types ◽  
Growth Potential ◽  
Matrix Proteins ◽  
Hepatoma Cell Line ◽  
Sequencing Data ◽  
Data Set

AbstractThe behavior of cells in vivo is complex and highly dynamic, as it results from an interplay between components of the intercellular matrix proteins with surface receptor and other microenvironmental cues. Although the effects of the cellular niche have been investigated for a number of cell types using different molecular approaches, comprehensive assessments of how the global transcriptome responds to 3D scaffolds composed of various extracellular matrix (ECM) constituents are still lacking. In this study, we explored the effect of the biomechanical parameters of Collagen I and Matrigel (ECM) on transcriptional gene regulation in a cell culture system. Using Huh-7 cells cultured on traditional cell culture plates or on the components of the ECM at different concentrations to modulate microenvironment properties, we have generated transcriptome sequencing data that may be further explored to understand the differentiation and growth potential of this cell for the development of 3D cultures. Assessment of the hepatocyte phenotype in relation to our transcriptomic data set would be very useful for the development of systems mimicking the in vivo structure and function of liver cells which still remains a challenge.

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