Cytotoxic activity assay from leaves and fruit extracts of Ficus aurata (Miq.) using brine shrimp lethality test method

Sponges are multicellular animals which have cytotoxic compounds. In this study, isolation and cytotoxic activity of Penares sp sponge from Menjangan island-water West Bali National Park have been carried out. The results showed that chloroform extract and GF V Penares sp fraction were toxic according to Brine Shrimp Lethality Test method (each 85% and 63.3% mortality at 25μg/mL concentration). The isolation result of GF V fraction was obtained a compound (Spot 4) that is active as cytotoxic agent against T47D cells (IC50 of 12.7 ug/mL). Spot 4 is a terpenoid compound which has conjugated double bonds.

Download Full-text

UJI AKTIVITAS SITOTOKSIK EKSTRAK METANOL BUAH KETUMBAR (Coriandrum sativum Linn) TERHADAP ARTEMIA SALINA LEACH DENGAN UJI BSLT (Brine Shrimp Lethality Test)

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v2i3.72 ◽

2020 ◽

Vol 2 (3) ◽

pp. 137-146

Author(s):

Mega Yulia ◽

Rani Anggraini ◽

Farizal Farizal

Keyword(s):

Cytotoxic Activity ◽

Brine Shrimp ◽

Methanol Extract ◽

Artemia Salina ◽

Test Method ◽

Coriandrum Sativum ◽

Brine Shrimp Lethality ◽

Activity Test ◽

Anti Cancer ◽

Expected Benefits

Coriander is a common herb can be used as traditional medicine. One of the expected benefits of Coriander fruits is an anti-cancer. This study aims to determine the cytotoxic activity of Coriander extract (Coriandrum sativum Linn) on shrimp larvae (Artemia salina Leach) with the BSLT (Brine Shrimp Lethality Test) method. Coriander fruits was extracted using methanol solvent and maceration method, then concentrated it with a rotary evaporator until a thick extract. Cytotoxic activity test used methanol extract with several concentration : 1,000 ppm, 100 ppm, 10 ppm, and 1 ppm. The result of this research reveal that methanol extract of Coriander fruits has cytotoxic activity with LC50 value is 32.35 ppm.

Download Full-text

Cytotoxic Activity of Two Limonoids Isolated From Swietenia mahagoni by Using Brine Shrimp Lethality Bioassay

Bangladesh Journal of Physiology and Pharmacology ◽

10.3329/bjpp.v24i1.5729 ◽

1970 ◽

pp. 1-6

Author(s):

AKM Shahidur Rahman ◽

AK Azad Chowdhury ◽

Hossain Reza ◽

Mohammad Shawkat Ali ◽

Manzurur Rahman Shah Choudhury ◽

...

Keyword(s):

Cytotoxic Activity ◽

Key Words ◽

Column Chromatography ◽

Brine Shrimp ◽

Meoh Extract ◽

Brine Shrimp Lethality ◽

Swietenia Mahagoni

Solvent partitioning followed by column chromatography of the MeOH extract of the seeds of Swietenia mahagoni afforded two limonoids, swietenolide (compound 1) and 2-hydroxy-3-O-tigloylswietenolide (compound 2), later one is new compound. The compounds were identified by spectroscopic means. The cytotoxic activity of these compounds was assessed by using the conventional brine shrimp lethality bioassay. While both compounds were found to have moderate cytotoxic activity, compound 2 displayed overall more potent activity than compound 1. Key words: Cytotoxic; Limonoid; Swietenia mahagoni; Brine shrimp lethality bioassay DOI: 10.3329/bjpp.v24i1.5729Bangladesh J Physiol Pharmacol 2008; 24(1&2) : 1-6

Download Full-text

UJI TOKSISITAS AKUT MINUMAN PROBIOTIK SIRSAK GUNUNG (Annona montana Macf.) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

Jurnal Inovasi Penelitian ◽

10.47492/jip.v1i5.174 ◽

2020 ◽

Vol 1 (5) ◽

pp. 941-948

Author(s):

Ambar Fidyasari ◽

Sentot Joko Raharjo ◽

Melani Setyowati

Keyword(s):

Acute Toxicity ◽

Traditional Medicine ◽

Brine Shrimp ◽

Experimental Methods ◽

Test Method ◽

Toxic Effects ◽

Brine Shrimp Lethality ◽

Lc50 Value ◽

A Value ◽

Acute Toxic

Soursop fruit (Annona montana Macf.) is one of the plants can be used as as traditional medicine. This plant contains terpenoid and acetogenin which can cause toxicity. The fruit has a flavor that is tasteless so the innovation becomes probiotic drinks. This drink has been proven as an antioxidant, antibacterial, antihyperuricemia and antidiarrheal. The aim of this study was to know about acute toxicity of probiotic drink of soursop juice using brine shrimp lethality test method which will be indicated by LC50 value. This study used experimental methods conducted in the Laboratory of Farmakoknosi. There are several variations in concentration in this study, namely 10000 ppm, 20000 ppm, 30000 ppm, 40000 ppm, 50000 ppm, 60000 ppm, 70000 ppm, 80000 ppm and replication was done 3 times with total number of test animals used was 270. The results showed that probiotic drink of soursop juice can provide acute toxic effects on test animals with LC50 value of 29717,23 ppm. LC50 values indicate that the mountain soursop probiotic drink is not potentially toxic because it has a value of >1000 ppm.

Download Full-text

UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.26017 ◽

2019 ◽

Vol 7 (3) ◽

pp. 265

Author(s):

Faradila Y. Karim ◽

Nickson J Kawung ◽

Billy Th. Wagey

Keyword(s):

Cytotoxic Activity ◽

Toxicity Test ◽

Brine Shrimp ◽

Bioactive Compound ◽

Artemia Salina ◽

Probit Analysis ◽

Thalassia Hemprichii ◽

Brine Shrimp Lethality ◽

A Value ◽

Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)

Download Full-text

The potential biopesticide toxicity test of Ipomoea batatas (L.) Lam (Purple Sweet Potato leaf extract) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test Method

International Journal of Scientific and Research Publications (IJSRP) ◽

10.29322/ijsrp.10.08.2020.p10428 ◽

2020 ◽

Vol 10 (8) ◽

pp. 212-217

Author(s):

Onik Anggita Sari ◽

Slamet Isworo

Keyword(s):

Sweet Potato ◽

Toxicity Test ◽

Brine Shrimp ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Artemia Salina ◽

Test Method ◽

Potato Leaf ◽

Brine Shrimp Lethality ◽

Purple Sweet Potato

Download Full-text

Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v22i2.42314 ◽

2019 ◽

Vol 22 (2) ◽

pp. 224-227

Author(s):

Md Sagar Hossain ◽

NM Mofiz Uddin Khan ◽

Md Saiful Quddus ◽

AM Sarwaruddin Chowdhury

Keyword(s):

Antimicrobial Activity ◽

Cytotoxic Activity ◽

Brine Shrimp ◽

Methanol Extract ◽

Soluble Fraction ◽

Pharmaceutical Journal ◽

Antimicrobial Screening ◽

Brine Shrimp Lethality ◽

Persicaria Perfoliata ◽

Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

Download Full-text

Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of Urtica Crenulata

Journal of Scientific Research ◽

10.3329/jsr.v2i1.2872 ◽

2009 ◽

Vol 2 (1) ◽

pp. 169-177

Author(s):

M. A. Rahman ◽

M. S. Rana ◽

M. M. Zaman ◽

S. A. Uddin ◽

R. Akter

Keyword(s):

Ascorbic Acid ◽

Free Radical ◽

Cytotoxic Activity ◽

Brine Shrimp ◽

Methanol Extract ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Brine Shrimp Lethality ◽

Brine Shrimp Lethality Assay ◽

Stem Extract

The antioxidant, antibacterial and cytotoxic properties of methanol extract of Urtica crenulata (syn: Laportea crenulata Gaud) stem has been investigated in the present study. The antioxidant and cytotoxic activity of the Urtica crenulata methanol extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay method, respectively. The antibacterial activity of the plant extract (500 μg/disc) was also carried out by disc diffusion technique. Stem extract showed DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging effect compared with ascorbic acid. IC50 value of ascorbic acid and stem extract was found 14.72 μg/ml and 1468.9 μg/ml, respectively. In antibacterial experiment, Urtica crenulata stem extract showed 8, 14 and 10 mm of diameter of zone inhibition against Salmonella typhi, Shigella flexneri and Shigella sonnei, respectively and 9 and 8 mm of diameter of zone inhibition against Bacillus subtilis and Bacillus cereus but no activity was observed against Staphylococcus aureus. In brine shrimp lethality assay, the LC50 value of the extract was found 104.0 μg/ml, which indicates that the extract has high cytoxic effect. The present study demonstrates that methanol extract of Urtica crenulata stem has significant cytotoxic effect. The extract also showed some moderate antibacterial and minimum significant antioxidant effects. Keywords: Urtica crenulata; Antioxidant; Antibacterial; Cytotoxic; BHT. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.2872 J. Sci. Res. 2 (1), 169-177 (2010)

Download Full-text