Utilization of Basil Leaf Extract as Anti-Mosquito Repellent: A Case Study of Total Mosquito Mortality (Aedes aegypti 3rd Instar)

ABSTRACT Aedes aegypti is the main vector of dengue virus carriers. One alternative to deal with these problem is by using insecticides derived from the leaves of breadfruit (Artocarous altilis) with the method of liquid electric. The research was purely experimental, with a posttest-only control group design with six variations of extract concentration of 9%, 13%, 20%, 30%, 44%, and 66% with 3 repetitions. The result of Kruskal Wallis test showed that there was a difference of the average number of Aedes aegypti mosquito mortality on various concentration of breadfruit leaf extract, that indicated by significance or probability value of 0.008 <0.05. The deadliest concentration of breadfruit leaf was 66% concentration with mosquito mortality of 83.3% from all mosquito test. The probit analysis obtained LC50 at concentration of 17.850% and LC90 at concentration of 98.348%. Keywords: Aedes aegypti, Breadfruit Leaf Extract, Liquid Electric ABSTRAK Aedes aegypti merupakan nyamuk yang menjadi vektor utama pembawa virus dengue. Salah satu alternatif lain untuk menangani permasalahan tersebut yaitu dengan menggunakan insektisida yang berasal dari tumbuhan atau nabati, yaitu daun sukun (Artocarous altilis) dengan metode elektrik cair. Jenis penelitian ini adalah eksperimen murni, dengan rancangan posttest only control group design dengan enam variasi konsentrasi ekstrak sebesar 9%, 13%, 20%, 30%, 44%, dan 66% dengan masing-masing 3 kali pengulangan. Hasil uji Kruskal Wallis menunjukkan bahwa ada perbedaan rata-rata jumlah kematian nyamuk Aedes aegypti pada berbagai konsentrasi ekstrak daun sukun, ditunjukkan dengan nilai signifikansi atau probabilitas adalah 0,008 < 0,05. Konsentrasi ekstrak daun sukun yang paling banyak mematikan nyamuk yaitu konsentrasi 66% dengan kematian nyamuk 83,3 % dari keseluruhan nyamuk. Uji analisis probit didapatkan LC50 pada konsentrasi 17.850% dan LC90 pada konsentrasi 98.348%. Kata kunci: Aedes aegypti, Ekstrak Daun Sukun, Elektrik Cair

Download Full-text

The Effectiveness of Citrus Hystrix As Rapelant against Aedes Aegypti

KnE Life Sciences ◽

10.18502/kls.v4i15.5728 ◽

2019 ◽

Author(s):

Anita Rosanty ◽

Reni Yunus ◽

Dian Yuniar SR

Keyword(s):

Experimental Study ◽

Aedes Aegypti ◽

Dengue Virus ◽

Leaf Extract ◽

Hemorrhagic Fever ◽

Life Cycles ◽

Case Study Design ◽

Aedes Mosquitoes ◽

Vector Mosquito

Indonesia is the country with the highest cases of dengue fever in Southeast Asia. The WHO estimates a 50–100 million dengue infections worldwide every year. Dengue Hemorrhagic Fever (DHF) is transmitted through the bite of female Aedes mosquitoes infected with the Dengue virus. Mosquitoes Aedes aegypti is the main vector of the dengue virus. At the time, there is no cure for dengue so that the control efforts are focused on breaking the chain of life cycles. One effort to prevent the transmission of the dengue virus is to avoid vector mosquito bites. Repellent can reduce exposure to the bite of mosquitoes infected with the dengue virus. The aim of this study was to determine the effectiveness of the extract of citrus hystrix leaf as a repellent against the Aedes aegypti mosquitoes. This research is a laboratory experimental study with a one-shot case study design. In this study, the extract of citrus hystrix solution was made at a concentration of 10%, 20%, and 30%, and then the extract solution was used as a stock to make a 100-gram base lotion that would be used as a repellent. Repellent effectiveness is seen from the percentage of repellent protection power. The percentage of repellant protection used with the basic ingredients of Citrus hystrix leaf extract at concentrations of 10%, 20%, and 30%, respectively, at 93.33% 94.67%, and 97.33%. The extract of citrus hystrix leaf was found to be effective as a repellent against Aedes aegypti mosquitoes.

Download Full-text

Utilization of Stem Bark and Leaves of Kluwih (Artocarpus Altilis Park) as an Anti-Mosquito Repellent: A Case Study of Total Mosquito Mortality (Anopheles Sp)

Indonesian Journal of Science and Technology ◽

10.17509/ijost.v5i3.25370 ◽

2020 ◽

Vol 5 (3) ◽

pp. 421-439

Author(s):

Betta Kurniawan ◽

Laila Puspita ◽

Yogi Kurnia ◽

Ismalia Husna ◽

Ali Rahmat ◽

...

Keyword(s):

Mortality Rate ◽

Stem Bark ◽

Alternative Solution ◽

Mosquito Repellent ◽

Leaf Extracts ◽

Artocarpus Altilis ◽

Mosquito Mortality ◽

Potential Use

The purpose of this study was to demonstrate an alternative solution as an insecticide from Indonesian natural ingredients. We investigated the potential use of Artocarpus altilis park (known as kluwih plants in Indonesia) for an anti-mosquito repellent because it contains flavonoids and saponins. The use of this type of insecticide is harmless, inexpensive, and largely available in Indonesia. In the experiment, we extracted bark and leaves of kluwih to obtain insecticidal material to repel Anopheles sp. To ensure the precise extraction and insecticidial effect, the experiments were conducted four times with three repetitions. For each experiment, we used 8.4 mL with the concentrations of 20, 30, and 40% of flavonoid. The results showed that the higher the concentrations of bark and leaf extracts of Artocarpus altilis park the higher the mortality rate of Anopheles sp is.

Download Full-text

Potensi Ekstrak Daun Marigold (Tagetes erecta L.) sebagai Larvasida terhadap Larva Aedes aegypti di Laboratorium

Jurnal Vektor Penyakit ◽

10.22435/vektorp.v12i2.898 ◽

2018 ◽

Vol 12 (2) ◽

pp. 109-114

Author(s):

Marini Marini ◽

Vivin Mahdalena ◽

Tanwirotun Ni'mah

Keyword(s):

Aedes Aegypti ◽

Leaf Extract ◽

Larval Mortality ◽

Ethanol Extract ◽

Probit Analysis ◽

Tagetes Erecta ◽

Mosquito Vectors ◽

Mosquito Repellent ◽

Tagetes Erecta L

Abstract Control of mosquito vectors by using natural ingredients is being developed intensively. Marigold (Tagetes erecta) was widely used as a mosquito repellent plant. The results of research before had found that marigold leaf extract has a repulsive effect on Aedes aegypti mosquitoes. This study aimed to identify the potential of marigold leaf extract as larvacide against Ae. aegypti larvae in the laboratory. The material used in this research was extracts of marigold leaves. Larvacide test was carried out by dissolving of extract in water at the concentration of 2,000 ppm, 4,000 ppm, 6,000 ppm, 8,000 ppm, and 10,000 ppm. The larvae used was Ae. aegypti larvae as many as 25 larvae each five treatment of replications. Probit analysis was conducted on larval mortality after 24 hours of exposure. The results of the probit analysis showed that the estimated concentration of extracts that could result in larval mortality up to 95% (LC95) was 7,456 ppm. From the result we can conclude that ethanol extract of marigold leaves (T. erecta L. ) has the biolarvacidal activity against larvae. Abstrak Pengendalian vektor nyamuk dengan memanfaatkan bahan alam makin banyak dikembangkan. Marigold (T. erecta) merupakan tumbuhan yang banyak dimanfaatkan sebagai tanaman pengusir nyamuk. Hasil penelitian sebelumnya menemukan bahwa ekstrak daun memiliki daya tolak terhadap nyamuk Ae. aegypti. Penelitian ini bertujuan untuk melihat potensi ekstrak daun marigold sebagai larvasida terhadap larva Ae. aegypti instar III di laboratorium. Bahan yang digunakan pada penelitian ini adalah ekstrak etanol daun marigold hasil ekstraksi dari penelitian sebelumnya. Uji larvasida dilakukan dengan melarutkan ekstrak dalam air pada konsentrasi 2.000 ppm, 4.000 ppm, 6.000 ppm, 8.000 ppm, dan 10.000 ppm. Larva yang digunakan yaitu larva Ae. aegypti sebanyak 25 larva tiap perlakuan dengan lima ulangan. Dilakukan analisis probit terhadap kematian larva setelah 24 jam paparan. Hasil analisa probit menunjukkan perkiraan konsentrasi ekstrak yang mampu mengakibatkan kematian larva hingga 95% (LC95 ) adalah 7.456 ppm. Ekstrak etanol daun marigold (T.erecta L.) memiliki potensi sebagai biolarvasida terhadap larva Ae. aegypti.

Download Full-text