Effects of Plectranthus barbatus leaf extract on survival, digestive proteases, midgut morphophysiology and gut microbiota homeostasis of Aedes aegypti larvae

Studies have suggested that modulation of gut microbiota is a viable therapeutic possibility for diabetes. This study evaluated the ability of an edible plant, Gongronema (G.) latifolium Benth (Asclepiadaceae), to modulate the gut microbiome and reduce blood glucose of alloxan-induced diabetic rats. Thirty (30) young, male, albino rats were divided into 6 groups of 5 rats each: Group 1 comprised normal rats; Groups 2 to 4, diabetic rats treated with 200, 400 and 800 mg/Kg body weight of hydro-alcoholic leaf extract, respectively; Group 5, diabetic rats treated with 0.2 mg/Kg glibenclamide (an anti-diabetic drug); and Group 6 comprised untreated diabetic rats. Following induction of diabetes with alloxan injections, the treatments were administered twice daily on a 12-hourly basis by orogastric intubation for 21 days. Thereafter, faecal samples were collected from the animals and subjected to metagenomic analysis, to ascertain the composition and relative abundance of the gut microbiota. There were five dominant bacterial phyla in the rat gut: Firmicutes, Bacteroidetes, Actinobacteria, Spirochaetea and Proteobacteria. Induction of diabetes resulted in observable dysbiosis in the rats. However, treatment of the diabetic rats with G. latifolium extract, ameliorated the state of dysbiosis and resulted in significant increase in species like Lactobacillus (L.) johnsonii, L. reuteri and Prevotella corpri, which are associated with improved glucose metabolism. The plant extract produced the best result at the dose of 400 mg/Kg. The results from this study show that G. latifolium may be used as a therapeutic option for restoration of the microbiome in diabetic patients.

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EFFECTIVENESS OF LOTION EXTRACT OF VARIOUS TYPE OF PLANTS AS Aedes aegypti MOSQUITO REPPELENT

Buletin Keslingmas ◽

10.31983/keslingmas.v39i4.6588 ◽

2020 ◽

Vol 39 (4) ◽

pp. 203-209

Author(s):

Agus - Subagiyo

Keyword(s):

Aedes Aegypti ◽

Leaf Extract ◽

Statistical Tests ◽

Hemorrhagic Fever ◽

Negative Control ◽

Toxic Substances ◽

Research Needs ◽

Protective Capacity ◽

Leaf Extracts ◽

The Difference

Effort to control DHF (Dengue Hemorrhagic Fever) vectors without toxic substances by using natural and enviromentally friendly materials was natural repellent made using plants that were widely available in the community.Many types of plants contain various secondary metabolite compound substances that have the potential to be used as natural insecticides, especially as repellent of the Aedes Aegypti mosquito. The research needs to be done to determine the effectiveness of basil leaves, citronella leaves, clove leaves and betel leaves as a repellent against Aedes Aegypti mosquitoes. This research method was an experiment in the laboratory. The materials used for this research were basil leaves, citronella leaves, clove leaves, and betel leaves. The extracted leaves were then made lotions with certain formulas, tested as a natural repellent against Aedes Aegypti mosquitoes. The concentrations used were 20%, 40% and 80% as well as negative control (0%) and positive control (brand x lotions). The number of mosquitoes used in the study were 25 x 4 treatments = 100 mosquitoes. The replication or repetition was done 10 times. To find out the difference in the number of Aedes Aegypti mosquitoes that perch on the hands of probandus which has been smeared with basil leaf, citronella leaf, clove leaf and betel leaf extracts uses Anova Statistical tests and kruskal wallis. To see the difference between concentrations was tested with LSD or Mann-Whitney test. The calculation result of the number of Aedes Aegypti mosquitoes that perch before and after applying the lotion, the lowest was the lotion of citronella leaf extract 0% (11); 20% (2); 40% (1); 80% (1); The highest protection capacity of extract lotion is citronella leaves with concetrations of 20% (81,1%); 40% (95%), 80%(98,2%); There were no significant differences between the lotion concentrations of 20% and 80%, while the concentration of 40% is significantly different. Then the LSD follow-up test finds that the protective capacity of lotion of clove leaf extract is negative and citronella leaf extract is positive. The most effective protection capacity against Aedes Aegypti mosquitoes was lotion of citronella leaf extract with concentration of 40% (95%) and a concentration of 80% (98,2%), proven by the value of protection capacity that is stable even though it has been used for up to 6 hours and can exceed the protection capacity of lotion x (90,3%). It is reccomended that further research needs to be developed to optimize the lotion of citronella leaf extract 40% and 80% with orgonolaptic test of color, aroma, stickness and comfort of its use.

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Efektivitas Ekstrak Daun Sirih (Piper betle L) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti

bionature ◽

10.35580/bionature.v20i1.9760 ◽

2019 ◽

Vol 20 (1) ◽

Author(s):

Amirullah Amirullah ◽

Nurhayu Malik ◽

Rosmaya Rosmaya

Keyword(s):

Aedes Aegypti ◽

Endemic Area ◽

Leaf Extract ◽

Allium Sativum ◽

Larval Mortality ◽

Hemorrhagic Fever ◽

Garlic Extract ◽

Piper Betle ◽

Mosquito Larvae ◽

Betel Leaf

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords: Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).

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Pengaruh ekstrak daun rambutan (Nephelium lappaceum L.) terhadap kematian larva nyamuk aedes aegypti

Jurnal SAGO Gizi dan Kesehatan ◽

10.30867/gikes.v1i1.291 ◽

2020 ◽

Vol 1 (1) ◽

pp. 73

Author(s):

Fajriansyah Fajriansyah

Keyword(s):

Aedes Aegypti ◽

Leaf Extract ◽

Statistical Data ◽

Human Life ◽

Mosquito Larvae ◽

Statistical Data Analysis ◽

Research Subjects ◽

A Value ◽

Nephelium Lappaceum ◽

Significant Difference

Background: The effort to control mosquitoes can be done by terminating the life cycle of mosquitoes, eradication of the larval sadium, namely larvicides. Rambutan leaves (Nephelium lappaceum L.) contain tannin compounds, saponins. Saponins are destroying red blood cells through the reaction of hemolysin, are toxic to cold-blooded animals, including cold-blooded insects, one of the insects that often interfere with human life is mosquitoes.Objectives: The purpose of this study was to determine the extract of rambutan leaves on the death of Aedes aegypti larvae Methods: This research is an experimental research. The object of research was Aedes aegypti mosquito larvae. The study was conducted at the writer home of Lamjamee Village, Banda Aceh. Research time is August 23-25, 2017. Type of research is an experimental study that is the effect of rambutan leaf extract on the death of Aedes aegypti larvae. The object of research is the population of Aedes aegypti larvae. The research subjects taken were Aedes aegypti mosquito larvae that lived because of the administration of rambutan leaf extract with a concentration of 5%, 10%, 15%. Statistical data analysis used Anova LSD test.Results: The results showed that the death of Aedes aegypti larvae by using rambutan leaf extract (Nephelium lappaceum L.) dose of 5% with an average of 19.7. Dose 10% had an average of 34.3, and a dose of 15% of the average mortality was 43.7. There is a significant difference between each dose of the mortality rate of Aedes aegypti larvae, with a value of F= 28.1 (F-count > F-table = 4.07).Conclusion: Various concentrations of the three treatments of rambutan leaf extract namely 5%, 10% and 15% are very effective against the death of Aedes aegypti larvae. Suggestions, can be used and practiced in everyday life, so as to reduce the use of chemical insecticides.

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OVIPOSITION PREFERENCE OF Aedes aegypti AGAINST VARIOUS LEAF EXTRACT AS AN ATRACTANT

BALABA JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA ◽

10.22435/blb.v11i1.4151.23-28 ◽

2015 ◽

Vol 11 (1) ◽

Author(s):

Sara Gustia Wibowo ◽

Endang Puji Astuti

Keyword(s):

Aedes Aegypti ◽

Leaf Extract ◽

Oviposition Preference

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The Effectiveness of Citrus Hystrix As Rapelant against Aedes Aegypti

KnE Life Sciences ◽

10.18502/kls.v4i15.5728 ◽

2019 ◽

Author(s):

Anita Rosanty ◽

Reni Yunus ◽

Dian Yuniar SR

Keyword(s):

Experimental Study ◽

Aedes Aegypti ◽

Dengue Virus ◽

Leaf Extract ◽

Hemorrhagic Fever ◽

Life Cycles ◽

Case Study Design ◽

Aedes Mosquitoes ◽

Vector Mosquito

Indonesia is the country with the highest cases of dengue fever in Southeast Asia. The WHO estimates a 50–100 million dengue infections worldwide every year. Dengue Hemorrhagic Fever (DHF) is transmitted through the bite of female Aedes mosquitoes infected with the Dengue virus. Mosquitoes Aedes aegypti is the main vector of the dengue virus. At the time, there is no cure for dengue so that the control efforts are focused on breaking the chain of life cycles. One effort to prevent the transmission of the dengue virus is to avoid vector mosquito bites. Repellent can reduce exposure to the bite of mosquitoes infected with the dengue virus. The aim of this study was to determine the effectiveness of the extract of citrus hystrix leaf as a repellent against the Aedes aegypti mosquitoes. This research is a laboratory experimental study with a one-shot case study design. In this study, the extract of citrus hystrix solution was made at a concentration of 10%, 20%, and 30%, and then the extract solution was used as a stock to make a 100-gram base lotion that would be used as a repellent. Repellent effectiveness is seen from the percentage of repellent protection power. The percentage of repellant protection used with the basic ingredients of Citrus hystrix leaf extract at concentrations of 10%, 20%, and 30%, respectively, at 93.33% 94.67%, and 97.33%. The extract of citrus hystrix leaf was found to be effective as a repellent against Aedes aegypti mosquitoes.

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