scholarly journals The Effectiveness of Citrus Hystrix As Rapelant against Aedes Aegypti

2019 ◽  
Author(s):  
Anita Rosanty ◽  
Reni Yunus ◽  
Dian Yuniar SR
Keyword(s):  
Experimental Study ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Life Cycles ◽  
Case Study Design ◽  
Aedes Mosquitoes ◽  
Vector Mosquito

Indonesia is the country with the highest cases of dengue fever in Southeast Asia. The WHO estimates a 50–100 million dengue infections worldwide every year. Dengue Hemorrhagic Fever (DHF) is transmitted through the bite of female Aedes mosquitoes infected with the Dengue virus. Mosquitoes Aedes aegypti is the main vector of the dengue virus. At the time, there is no cure for dengue so that the control efforts are focused on breaking the chain of life cycles. One effort to prevent the transmission of the dengue virus is to avoid vector mosquito bites. Repellent can reduce exposure to the bite of mosquitoes infected with the dengue virus. The aim of this study was to determine the effectiveness of the extract of citrus hystrix leaf as a repellent against the Aedes aegypti mosquitoes. This research is a laboratory experimental study with a one-shot case study design. In this study, the extract of citrus hystrix solution was made at a concentration of 10%, 20%, and 30%, and then the extract solution was used as a stock to make a 100-gram base lotion that would be used as a repellent. Repellent effectiveness is seen from the percentage of repellent protection power. The percentage of repellant protection used with the basic ingredients of Citrus hystrix leaf extract at concentrations of 10%, 20%, and 30%, respectively, at 93.33% 94.67%, and 97.33%. The extract of citrus hystrix leaf was found to be effective as a repellent against Aedes aegypti mosquitoes.

Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 213-218
Author(s):  
Suresh Chand Kaushik ◽  
Sukhvir Singh ◽  
Purnima Srivastava ◽  
R. Rajendran
Keyword(s):  
Early Intervention ◽  
Early Detection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Transmission Season ◽  
Aedes Mosquitoes ◽  
Alternative Approach ◽  
Human Sera ◽  
Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Nurul Qamila ◽  
Agel Vidian Krama
Keyword(s):  
Public Health ◽  
Spatial Analysis ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Spatial Pattern ◽  
Dengue Hemorrhagic Fever ◽  
Health Problem ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

scholarly journals The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti

2020 ◽  
Vol 21 (20) ◽  
pp. 7520
Author(s):  
Lucky R. Runtuwene ◽  
Shuichi Kawashima ◽  
Victor D. Pijoh ◽  
Josef S. B. Tuda ◽  
Kyoko Hayashida ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Cell Line ◽  
Dengue Infection ◽  
Fruit Fly ◽  
Initiation Factor ◽  
Poly I:C ◽  
Gene Products ◽  
Vector Mosquito

Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post dengue infection to those of uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes a member of the heat shock 20 protein (HSP20) family, was upregulated following dengue virus type 2 (DENV-2) infection in vivo. The transcripts of this gene did not exhibit differential accumulation in mosquitoes exposed to insecticides or pollutants. The induction and overexpression of l(2)efl gene products using poly(I:C) resulted in decreased DENV-2 replication in the cell line. In contrast, the RNAi-mediated suppression of l(2)efl gene products resulted in enhanced DENV-2 replication, but this enhancement occurred only if multiple l(2)efl genes were suppressed. l(2)efl homologs induce the phosphorylation of eukaryotic initiation factor 2α (eIF2α) in the fruit fly Drosophila melanogaster, and we confirmed this finding in the cell line. However, the mechanism by which l(2)efl phosphorylates eIF2α remains unclear. We conclude that l(2)efl encodes a potential anti-dengue protein in the vector mosquito.

scholarly journals The influence of natural environmental condition towards the existence of vector's larva of dengue hemorrhagic fever: Aedes Aegypti

2017 ◽  
Author(s):  
Susiana Nugraha
Keyword(s):  
Logistic Regression ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Water Reservoir ◽  
Hemorrhagic Fever ◽  
Morbidity Rate ◽  
Tropical Regions ◽  
Water Replacement ◽  
Dengue Outbreaks

Dengue hemorrhagic fever is a severe and fatal infection that occurs in tropical regions such as Indonesia. In 2014, recorded that dengue morbidity rate was 5.17 per 100,000 inhabitants (approximately 13031 cases) with mortality rate of 0.84% (110 deaths). Demographic and societal changes such as population growth, urbanization, and modern transportation appear to play an important role in the increased incidence and geographical spread of dengue virus. Aedes aegypti, the urban yellow fever mosquito, is also the principal dengue-carrying vector. The Aedes aegypti mosquitoes as a vector of dengue virus normally live and breed in clean water reservoirs that are not directly related to the land such as: bath, bird drinks, water pot, water jars / barrel, cans, old tires, etc. In Indonesia, dengue outbreaks often occur when the seasons change from dry to rain hor vice versa. This study aimed to figure out the influence of natural environment and the existence of the vector’s larva. A logistic regression was performed to ascertain the effects of temperature, humidity, water replacement and the existence of water reservoir on the likelihood of the existence of the vector’s larva . The logistic regression model was statistically significant, p < .005 and the model explained 69% (Nagelkerke R2). This finding shows that the existence of the mosquito’s larva, influenced by temperature, water replacement activity and the existence of water reservoir. Health education about vector control and environmental engineering are necessary to break the chain of mosquito breeding.

scholarly journals EFFECTIVENESS OF LOTION EXTRACT OF VARIOUS TYPE OF PLANTS AS Aedes aegypti MOSQUITO REPPELENT

2020 ◽  
Vol 39 (4) ◽  
pp. 203-209
Author(s):  
Agus - Subagiyo
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Statistical Tests ◽  
Hemorrhagic Fever ◽  
Negative Control ◽  
Toxic Substances ◽  
Research Needs ◽  
Protective Capacity ◽  
Leaf Extracts ◽  
The Difference

Effort to control DHF (Dengue Hemorrhagic Fever) vectors without toxic substances by using natural and enviromentally friendly materials was natural repellent made using plants that were widely available in the community.Many types of plants contain  various secondary metabolite compound substances that have the potential to be used as natural insecticides, especially as repellent of the Aedes Aegypti mosquito. The research needs  to be done to determine the effectiveness of basil leaves, citronella leaves, clove leaves and betel leaves as a repellent against Aedes Aegypti mosquitoes. This research method was an experiment in the laboratory. The materials used for this research were basil leaves, citronella leaves, clove leaves, and betel leaves. The extracted leaves were then made lotions with certain formulas, tested as a natural repellent against Aedes Aegypti mosquitoes. The concentrations used were 20%, 40% and 80% as well as negative control (0%) and positive control (brand x lotions). The number of mosquitoes used in the study were 25 x 4 treatments = 100 mosquitoes. The replication or repetition was done 10 times. To find out the difference in the number of Aedes Aegypti mosquitoes that perch on the hands of probandus which has been smeared with basil leaf, citronella leaf, clove leaf and betel leaf extracts uses Anova Statistical tests and kruskal wallis. To see the difference between concentrations was tested with LSD or Mann-Whitney test. The calculation result of the number of Aedes Aegypti mosquitoes that perch before and after applying the lotion, the lowest was the lotion of citronella leaf extract 0% (11); 20% (2); 40% (1); 80% (1); The highest protection capacity of extract lotion is citronella leaves with concetrations of 20% (81,1%); 40% (95%), 80%(98,2%); There were no significant differences between the lotion concentrations of 20% and 80%, while the concentration of 40% is significantly different. Then the LSD follow-up test finds that the protective capacity of lotion of clove leaf extract is negative and citronella leaf extract is positive. The most effective protection capacity against Aedes Aegypti mosquitoes was lotion of citronella leaf extract with concentration of 40% (95%) and a concentration of 80% (98,2%), proven by the value of protection capacity that is stable even though it has been used for up to 6 hours and can exceed the protection capacity of lotion x (90,3%). It is reccomended that further research needs to be developed to optimize the lotion of citronella leaf extract 40% and 80% with orgonolaptic test of color, aroma, stickness and comfort of its use.

scholarly journals Efektivitas Ekstrak Daun Sirih (Piper betle L) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti

bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Rosmaya Rosmaya
Keyword(s):  
Aedes Aegypti ◽  
Endemic Area ◽  
Leaf Extract ◽  
Allium Sativum ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Garlic Extract ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords:  Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).

DETECTION OF DENGUE TRANSOVARIAL VIRUS FOR AIDES AEGYPTI MOSQUITOES IN ENDEMIC AREAS OF DENGUE HEMORRHAGIC FEVER

2019 ◽  
Vol 3 (9) ◽  
Author(s):  
Suparmin . ◽  
Arif Widyanto ◽  
Ismi Rajiani
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Positive Sample ◽  
Elisa Method ◽  
Central Java ◽  
Vector Population ◽  
The Government ◽  
Central Java Province

Background: The incidence rate (IR) of Dengue Hemorrhagic Fever per 100,000 population in Central Java has increased dramatically in five years. Banyumas Regency is an endemic area of ​​dengue hemorrhagic fever in Central Java Province. The cause of dengue hemorrhagic fever is four dengue viruses known as DEN-1, DEN-2, DEN-3, and DEN-4. The purpose of this research is to detect transovarial dengue virus in Aedes aegypti mosquito in Banyumas Regency. Methods: The study was conducted by installing ovitrap in Mersi Village, East Purwokerto District, and in Pliken Village, Kembaran District, Banyumas Regency. The study was conducted by installing ovitrap in Mersi Village (9 samples) and Pliken Village (3 samples). The eggs obtained are hatched into larvae in the laboratory. The 3rd and 4th instar larvae were examined for the presence of dengue virus using the Elisa method. Result: The results showed that in Mersi Village, there were two positive samples, and in Pliken Village, there was one positive sample containing dengue virus. The presence of the dengue virus found in the hatched Aedes aegypti larvae (F1) indicates that there has been a transovarial dengue virus from the parent mosquito in its offspring. Conclusion: The study concluded that in Banyumas District, a dengue virus transovarial had occurred in Aedes aegypti mosquitoes. The government and the community should increase efforts to eradicate mosquito nests (dengue hemorrhagic fever) to reduce the vector population of dengue hemorrhagic fever so that cases of dengue hemorrhagic fever can be eliminated.    Keyword: Transovarial dengue virus, Aedes aegypti, Dengue Hemorrhagic Fever

scholarly journals EFEKTIVITAS ABATE DAN EKSTRAK DAUN SIRIH (Piper betle) DALAM MEMATIKAN LARVA Aedes Aegypti L INSTAR III

Masker Medika ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 226-232
Author(s):  
Triana Oktaviani ◽  
Zairinayati Zairinayati
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf ◽  
Preventive Effort ◽  
Randomized Design ◽  
Natural Insecticides ◽  
Completely Randomized Design

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle).     Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.

scholarly journals THE EFFECTIVENESS TEST OF BETEL LEAF EXTRACT TOWARD THE DEVELOPMENT OF AEDES AEGYPTI SP LARVAE

Biospecies ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 1-7
Author(s):  
Firza Khairullah Sembiring ◽  
Riyani Susan BT Hasan
Keyword(s):  
Aedes Aegypti ◽  
Experimental Method ◽  
Leaf Extract ◽  
Sampling Method ◽  
Total Mortality ◽  
Hemorrhagic Fever ◽  
Betel Leaf ◽  
Significant Difference ◽  
Post Test ◽  
Confidence Index

This research was conducted to determine the effectiveness of betel leaf extract conducted at a concentration of 2.5%, 5.0%, 7.5%, 10% as larvicide toward the development of Aedes Aegypti Sp. Aedes Aegypti Sp larvae are dangerous vectors in Dengue Hemorrhagic Fever (DHF) in humans. The method in this research was carried out on an experimental method by using a post-test only design and sampling with a purposive sampling method. The effectiveness of betel leaf extract larvicide on the development of Aedes Aegypti Sp larvae was carried out by mixing betel leaf extract with several concentrations in one container Aedes Aegypti Sp larvae inside. The results obtained in this research were carried out by the Kruskal-Wallis test, the test found that there was a significant difference because it was given a 95% confidence index. The larvae treated with betel leaf extract with a concentration of 10% had the highest effectiveness in stopping the development of Aedes Aegypti Sp larvae, which proved at the mortality at a concentration of 10%, found total mortality of 20 larvae in 8 hours of treatment. Betel leaf extract with a concentration of 10% had the highest effectiveness of discontinuing the development of Aedes Aegypti Sp larvae.

Sign in / Sign up

Export Citation Format

Share Document