Antibacterial Activity of Ethanol Extract and Ethyl Acetate of Ginseng Bugis (Talinum Panicullatum Gaertn.) Leaves Against Staphylococcus Aureus and Escherichia coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10; and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is 7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

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SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

Journal of Pharmacopolium ◽

10.36465/jop.v1i1.394 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Vera Nurviana

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Diffusion Method ◽

Water Fraction ◽

Tetracycline Hcl

Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl. Keywords: Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK n-HEKSAN, ETIL ASETAT DAN ETANOL KULIT BATANG KAYU EBONI (Diospyros celebica Bakh.) MENGGUNAKAN METODE DIFUSI

KOVALEN ◽

10.22487/kovalen.2019.v5.i3.11540 ◽

2019 ◽

Vol 5 (3) ◽

pp. 233-239

Author(s):

Ni Luh Sumitriasih ◽

Ahmad Ridhay ◽

Indriani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Tree Bark ◽

Diffusion Method ◽

Well Test ◽

Activity Test ◽

Ethanol Extracts

A study was conducted on the antibacterial activity test on n-hexane, ethyl acetate, and ethanol extracts of ebony bark (Diospyros celebica Bakh.) using a diffusion method. The purpose of this research is to know the antibacterial activity of ebony bark against Staphylococcus aureus and Escherichia coli bacteria using n-hexane, ethyl acetate dan ethanol. The method of extraction used in this research was maceration method with three polarity levels of solvent. The antibacterial activity test was performed by diffusion-well test. The results showed that ethyl acetate extract gave the highest inhibition of 26.14 mm in gram-positive bacteria (Staphylococcus aureus) and ethanol extract of 25.97 mm in gram-negative bacteria (Escherichia coli). Keywords: Ebony (Diospyros celebica. Bakh), Tree Bark, Inhibitory, Antibacterial

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Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽

10.35799/jm.8.2.2019.23514 ◽

2019 ◽

Vol 8 (2) ◽

pp. 42

Author(s):

Florentin Natalia Melsadalam ◽

Dewa Gede Katja ◽

Meiske S. Sangi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Leaf Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Inhibitory Zone ◽

Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

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PENENTUAN AKTIVITAS EKSTRAK ETANOL CABAI MERAH DALAM MENGHAMBAT PERTUMBUHAN BAKTERI

Jurnal Penelitian Saintek ◽

10.21831/jps.v26i1.39859 ◽

2021 ◽

Vol 26 (1) ◽

pp. 64-73

Author(s):

Alfi Sapitri ◽

Eva Diansari Marbun ◽

Ulfayani Mayasari

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Clear Zone ◽

Capsicum Annum ◽

Activity Test ◽

Mueller Hinton ◽

Mueller Hinton Agar ◽

Escherichia Coli Bacteria

Penelitian ini bertujuan untuk melakukan uji fitokimia terhadap ekstrak etanol Cabai merah (Capsicum annum L.) dan uji aktivitasnya terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian aktivitas antibakteri ini menggunakan metode difusi agar dengan menggunakan kertas cakram dengan tiga kali pengulangan. Ekstrak etanol cabai merah dibuat dengan konsentrasi 45, 60, 75, dan 90%. Uji aktivitas antibakteri dilakukan dengan metode Kirby Bauer. Media yang digunakan sebagai uji antibakteri pada penelitian ini yaitu Mueller-Hinton Agar (MHA). Hasil skrining fitokimia terhadap ekstrak etanol cabai merah menunjukkan adanya senyawa metabolit sekunder. Senyawa metabolit sekunder tersebut yaitu alkaloid, flavonoid, tanin, saponin, dan Steroid/triterpenoid. Steroid berfungsi sebagai antimikroba. Hasil penelitian ini juga mengungkapkan adanya kandungan senyawa capsaicin yang terkenal sebagai senyawa aktif antimikroba. Ekstrak etanol cabai merah memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli zona bening terendah pada konsentrasi 45% dan zona bening tertinggi pada konsentrasi 90%. Dapat disimpulkan bahwa ekstrak cabai merah memiliki aktivitas antibakteri terhadap penghambatan pertumbuhan bakteri.THE ABILITY OF RED CHILI ETHANOL EXTRACT IN INHIBITHING THE GROWTH OF BACTERIAThis study aimed to perform a phytochemical test on the ethanolic extract of red chili (Capsicum annum L.) and test its activity against the growth of Staphylococcus aureus and Escherichia coli bacteria. This antibacterial activity test used the agar diffusion method using disc paper with three repetitions. Red chili ethanol extract was made with concentrations of 45%, 60%, 75% and 90%. The antibacterial activity test was carried out using the Kirby Bauer method. The medium used as an antibacterial test in this study was Mueller-Hinton Agar (MHA). The results of phytochemical screening of red chili ethanol extract showed the presence of secondary metabolites. The secondary metabolites are alkaloids, flavonoids, tannins, saponins, and steroids/triterpenoids. Steroids serve as antimicrobials. The results of this study also revealed the presence of capsaicin compounds which are known as active antimicrobial compounds. Red chili ethanol extract had antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the lowest clear zone at a concentration of 45% and the highest clear zone at a concentration of 90%. It can be concluded that red chili extract has antibacterial activity against bacterial growth inhibition.

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UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v2i1.201 ◽

2019 ◽

Vol 2 (1) ◽

pp. 14-18

Author(s):

Firdaus Fahdi ◽

Harwitavia Harwitavia ◽

Herviani Sari

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Leaf Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Antibiotic Drugs ◽

Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

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AKTIVITAS ANTIBAKTERI FRAKSI ALGA Turbinaria ornata (Turner) J. Agardh YANG DIPEROLEH DARI PERAIRAN TELUK MANADO

PHARMACON ◽

10.35799/pha.8.2019.29298 ◽

2019 ◽

Vol 8 (2) ◽

pp. 325

Author(s):

Nadia Adam ◽

Widya A. Lolo ◽

Sri Sudewi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Raw Materials ◽

Diffusion Method ◽

Marine Biota ◽

Turbinaria Ornata ◽

E Coli ◽

Escherichia Coli Bacteria

ABSTRACT Marine biota such as algae has been developed to be used as a source of medicinal raw materials in the pharmaceutical industry. Extracs from Turbinaria ornata algae J. Agardh showed antibacterial activity. This study aims to determine the antibacterial activity of the Turbinaria ornata (Turner) J. Agardh fraction obtained from the waters of the Manado Bay against the growth of Staphylococcus aureus and Escherichia coli bacteria. The method used in this study is an experimental laboratory. Turbinaria ornata (Turner) J. Agardh was extracted by maceration method with ethanol 96% solvent followed by fractionation and antibacterial activity testing with diffusion method. Antibacterial activity was determined based on the results of the clear zone measurements formed around the disc paper. The results showed that the methanol at a concentration of 10%, 20%, 30%, fractions of 30% ethyl acetate, and fractions of 20% and 30% n-hexane, have antibacterial activity which is categorized strongly in both bacteria of S. aureus and E. coli, while ethyl acetate fractions with a concentration of 10% and 20% againts S.aureus is in the moderate category. Likewise the antibacterial activity of n-hexane fraction with a concentration of 10% againts E. coli was included in the medium category. Keywords: Turbinaria ornata (Turner) J. Agardh, antibacterial, Staphylococcus aureus, Escherichia coli ABSTRAK Biota laut seperti alga telah banyak dikembangkan untuk digunakan sebagai sumber bahan baku obat dalam industri farmasi. Ekstrak dari alga Turbinaria ornata (Turner) J. Agardh menunjukan adanya aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri fraksi Turbinaria ornata (Turner) J. Agardh yang diperoleh dari perairan Teluk Manado terhadap pertumbuhan bakteri Staphylococus aureus dan Escherichia coli. Metode yang digunakan dalam penelitian ini adalah eksperimental laboratorium. Turbinaria ornata (Turner) J. Agardh diekstraksi dengan metode maserasi dengan pelarut etanol 96% dilanjutkan dengan tahap fraksinasi dan pengujian aktivitas antibakteri dengan metode difusi. Aktivitas antibakteri ditentukan berdasarkan hasil pengukuran zona bening yang terbentuk disekitar kertas cakram. Hasil penelitian menunjukkan bahwa fraksi metanol pada konsentrasi 10%. 20% ,30%, fraksi etil asetat konsentrasi 30%, dan fraksi n-heksana pada konsentrasi 20%, 30% memiliki aktivitas antibakteri yang dikategorikan kuat pada kedua bakteri yaitu S. aureus dan E. coli, sedangkan fraksi etil asetat dengan konsentrasi 10% dan 20% pada bakteri S. aureus termasuk dalam kategori sedang. Begitu pula aktivitas antibakteri fraksi n-heksan dengan konsentrasi 10% terhadap E. coli termasuk dalam kategori sedang. Kata kunci : Turbinaria ornata (Turner) J. Agardh, antibakteri, Staphylococus aureus, Escherichia coli.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI ALGA Caulerpa racemosa DARI PERAIRAN PULAU MANTEHAGE MINAHASA UTARA TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus

PHARMACON ◽

10.35799/pha.10.2021.34042 ◽

2021 ◽

Vol 10 (2) ◽

pp. 905

Author(s):

Yosefa E.H. Ritan ◽

Defny S. Wewengkang ◽

Jainer Pasca Siampa

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Inhibition Zone ◽

Chloroform Fraction ◽

Caulerpa Racemosa ◽

Gram Positive Bacteria ◽

Chemical Content ◽

Escherichia Coli Bacteria

ABSTRACTAlgae Caulerpa racemosa are able to produce chemical content that leave the potential to be antibacterial. The purpose of this study was to determine whether the extracts and fractions of algae Caulerpa racemosa had antibacterial activity against the Gram-negative bacteria Escherichia coli and the Gram-positive bacteria Staphylococcus aureus. The sample was extracted using the maceration method with 95% ethanol and the fractionation method used was liquid-liquid fractionation. Antibacterial activity testing was carried out using the disc diffusion agar method. The results obtained from the antibacterial activity test on Escherichia coli bacteria produce an inhibition zone in all extracts and fractions used while Staphylococcus aureus bacteria produced an inhibition zone for all extracts and fractions, in ethanol extract with inhibitory value 6.72mm, n-hexane fraction 7.53mm, chloroform fraction 6.93mm and fraction methanol 7.32mm, respectively. The extracts and fractions of algae Caulerpa racemosa samples showed no activity against Escherichia coli, while Staphylococcus aureus has performed antibacterial activity with moderate category. The conclusion of this study, extracts and fractions of algae caulerpa racemosa had no antibacterial activity on the bacteria Escherichia coli while bacteria Staphylococcus aureus had antibacterial activity with moderate inhibition.Keywords: Algae Caulerpa racemosa, Antibacterial, Escherichia coli, Staphylococcus aureusABSTRAKAlga Caulerpa racemosa mampu menghasilkan kandungan kimia yang berpotensi sebagai antibakteri. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak dan fraksi dari alga Caulerpa racemosa di Perairan Pulau Mantehage Minahasa Utara terhadap bakteri Escherichia coli sebagai bakteri Gram-negatif dan Staphylococcus aureus sebagai bakteri Gram-positif. Sampel diekstraksi dengan menggunakan metode maserasi dengan pelarut etanol 95% dan metode fraksinasi yang digunakan yaitu fraksinasi cair-cair. Pengujian aktivitas antibakteri yang dilakukan menggunakan metode difusi agar. Hasil yang didapat dari uji aktivitas antibakteri pada bakteri Escherichia coli tidak menghasilkan zona hambat pada semua ekstrak dan fraksi yang digunakan sedangkan pada bakteri Staphylococcus aureus menghasilkan zona hambat pada semua ekstrak dan fraksi yaitu, ekstrak etanol dengan daya hambat 6,72mm, fraksi n-heksan 7,53mm, fraksi kloroform 6,93mm dan fraksi metanol 7,32mm. Kesimpulan dari penelitian ini, ekstrak dan fraksi dari sampel alga Caulerpa racemosa tidak memiliki aktivitas pada bakteri Escherichia coli sedangkan pada bakteri Staphylococcus aureus memiliki aktivitas antibakteri dengan daya hambat yang sedang.Kata Kunci: Alga Caulerpa racemosa, Antibakteri, Escherichia coli, staphylococcus aureus

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UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v2i1.77 ◽

2019 ◽

Vol 2 (1) ◽

pp. 19-23

Author(s):

Firdaus Fahdi ◽

Harwitavia Harwitavia ◽

Herviani Sari

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Leaf Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Antibiotic Drugs ◽

Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

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Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

Jurnal Riset Kimia ◽

10.25077/jrk.v10i2.335 ◽

2019 ◽

Vol 10 (2) ◽

pp. 94-98

Author(s):

Adlis Santoni ◽

Mai Efdi ◽

Akmel Suhada

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Inhibition Zone ◽

Endemic Plant ◽

Dpph Method ◽

Hexane Extracts

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibition zone 10 mm at concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

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