scholarly journals PENENTUAN AKTIVITAS EKSTRAK ETANOL CABAI MERAH DALAM MENGHAMBAT PERTUMBUHAN BAKTERI

2021 ◽  
Vol 26 (1) ◽  
pp. 64-73
Author(s):  
Alfi Sapitri ◽  
Eva Diansari Marbun ◽  
Ulfayani Mayasari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Clear Zone ◽  
Capsicum Annum ◽  
Activity Test ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Escherichia Coli Bacteria

Penelitian ini bertujuan untuk melakukan uji fitokimia terhadap ekstrak etanol Cabai merah (Capsicum annum L.) dan uji aktivitasnya terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian aktivitas antibakteri ini menggunakan metode difusi agar dengan menggunakan kertas cakram dengan tiga kali pengulangan. Ekstrak etanol cabai merah dibuat dengan konsentrasi 45, 60, 75, dan 90%. Uji aktivitas antibakteri dilakukan dengan metode Kirby Bauer. Media yang digunakan sebagai uji antibakteri pada penelitian ini yaitu Mueller-Hinton Agar (MHA). Hasil skrining fitokimia terhadap ekstrak etanol cabai merah menunjukkan adanya senyawa metabolit sekunder. Senyawa metabolit sekunder tersebut yaitu alkaloid, flavonoid, tanin, saponin, dan Steroid/triterpenoid. Steroid berfungsi sebagai antimikroba. Hasil penelitian ini juga mengungkapkan adanya kandungan senyawa capsaicin yang terkenal sebagai senyawa aktif antimikroba. Ekstrak etanol cabai merah memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli zona bening terendah pada konsentrasi 45% dan zona bening tertinggi pada konsentrasi 90%. Dapat disimpulkan bahwa ekstrak cabai merah memiliki aktivitas antibakteri terhadap penghambatan pertumbuhan bakteri.THE ABILITY OF RED CHILI ETHANOL EXTRACT IN INHIBITHING THE GROWTH OF BACTERIAThis study aimed to perform a phytochemical test on the ethanolic extract of red chili (Capsicum annum L.) and test its activity against the growth of Staphylococcus aureus and Escherichia coli bacteria. This antibacterial activity test used the agar diffusion method using disc paper with three repetitions. Red chili ethanol extract was made with concentrations of 45%, 60%, 75% and 90%. The antibacterial activity test was carried out using the Kirby Bauer method. The medium used as an antibacterial test in this study was Mueller-Hinton Agar (MHA). The results of phytochemical screening of red chili ethanol extract showed the presence of secondary metabolites. The secondary metabolites are alkaloids, flavonoids, tannins, saponins, and steroids/triterpenoids. Steroids serve as  antimicrobials. The results of this study also revealed the presence of capsaicin compounds which are known as active antimicrobial compounds. Red chili ethanol extract had antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the lowest clear zone at a concentration of 45% and the highest clear zone at a concentration of 90%. It can be concluded that red chili extract has antibacterial activity against bacterial growth inhibition.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK n-HEKSAN, ETIL ASETAT DAN ETANOL KULIT BATANG KAYU EBONI (Diospyros celebica Bakh.) MENGGUNAKAN METODE DIFUSI

KOVALEN ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 233-239
Author(s):  
Ni Luh Sumitriasih ◽  
Ahmad Ridhay ◽  
Indriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Tree Bark ◽  
Diffusion Method ◽  
Well Test ◽  
Activity Test ◽  
Ethanol Extracts

A study was conducted on the antibacterial activity test on n-hexane, ethyl acetate, and ethanol extracts of ebony bark (Diospyros celebica Bakh.) using a diffusion method. The purpose of this research is to know the antibacterial activity of ebony bark against Staphylococcus aureus and Escherichia coli bacteria using n-hexane, ethyl acetate dan ethanol. The method of extraction used in this research was maceration method with three polarity levels of solvent. The antibacterial activity test was performed by diffusion-well test. The results showed that ethyl acetate extract gave the highest inhibition of 26.14 mm in gram-positive bacteria (Staphylococcus aureus) and ethanol extract of 25.97 mm in gram-negative bacteria (Escherichia coli). Keywords: Ebony (Diospyros celebica. Bakh), Tree Bark, Inhibitory, Antibacterial

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals FORMULATION AND ANTIBACTERIAL ACTIVITY TEST OF HAND SANITIZER ETHANOL EXTRACT OF BASIL (Ocimum Sanctum. L) AGAINST Escherichia coli dan Streptococcus Aureus

2020 ◽  
Vol 15 (2) ◽  
pp. 228
Author(s):  
Nurfiddin Farid ◽  
Nurhikma Nurhikma ◽  
Suhartina Hamzah ◽  
Muhammad Yusuf ◽  
Rahmania Rahmania
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Ocimum Sanctum ◽  
Hand Sanitizer ◽  
Activity Test ◽  
Before And After ◽  
And Staphylococcus Aureus

Basil leaves (Ocimum sanctum L) Against to inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria to increase its effectiveness, it is made in practical and easy-to-use preparations. One of the effective dosage forms for topical use is Hand Sanitizer. The factor that influences the quality of gel preparations is Carbopol as a gelling agent. To know the antibacterial effect and ethanol extract concentration of basis leaves (Ocimum sanctum L). preparation of Hand sanitizer, in which the selection and type of carbopol concentration will determine the stability of the gel formed. Basil leaf extract was obtained by maceration method using 96% ethanol solvent. The product is formulation in the form a gel Hand sanitizer with the addition of carbopol as a gelling agent with a concentration of 1,5% this type of research is  laboratory Experiment with Stability Test, Organoleptic Test, pH, Homogeneity and Scattering power and Antibacterial Test on Escherichia coli and Staphylococcus aureus. For testing antibacterial activity the method used is the diffusion method. Form the research results obtained antibacterial Hand sanitizer preparations with concentration of 1%, 3% and 5%  showed changes in odor , color and shape before and after storage at pH 4,8- 6,4. Organoleptic  testing showed no changes in odor, color and shape before and after accelerated storage. pH testing shows charges before and after storage are caused by temperature light factors. Homogeneity testing showed no changes before and after storage. Scatter tests indicate changes before and after storage caused by temperature light factors. Antibacterial activity test shows that the formulation of Hand sanitizer ethanol extract of basil leaves (Ocimum sanctum L) with a concentration of 1%, 3% and 5%. can inhibit the growth of Escherichia coli and Staphylococcus aureus with Medium -Strong inhibitory zones. From the results of research conducted it was concluded that the antibacterial Hand sanitizer ethanol of basis was physically and chemically stable for Organiletic, pH, Homogeneity and Scattering effects.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS Mycale Vansoesti DARI PERAIRAN PULAU MANTEHAGE MINAHASA UTARA TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 889
Author(s):  
Brigita Michelle Luntungan ◽  
Defny S. Wewengkang ◽  
Erladys Rumondor
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Chloroform Fraction ◽  
Marine Biota ◽  
Methanol Fraction ◽  
A Value ◽  
Activity Test ◽  
And Staphylococcus Aureus

ABSTRACTSponges are multi-cell marine biota whose tissue and organ functions are very simple. In the development of medicine, sponges have been shown to contain active compounds as guiding compounds in the synthesis of the latest drugs. The purpose of this study was to determine the antibacterial activity of the extracts and fractions of the sponge Mycale vansoesti collected from the waters of Mantehage Island against Escherichia coli and Staphylococcus aureus bacteria. The sponge samples were extracted using the maceration method with 95% ethanol. The antibacterial activity test used the disc diffusion agar method of Kirby and Bauer. The results showed that Mycale vansoesti sponge produced antibacterial activity in all extracts and fractions used namely ethanol extract, chloroform fraction, n-hexane fraction, and methanol fraction against Escherichia coli and Staphylococcus aureus bacteria with a value of about 6.63-8.82 mm and included in the moderate category of inhibition, this is proven by the formation of a clear zone around the disc. Keywords: Mycale vansoesti, Antibacterial, Staphylococcus aureus, Escherichia coli ABSTRAKSpons merupakan biota laut multi sel yang fungsi jaringan dan organnya sangat sederhana. Dalam perkembangan pengobatan, spons terbukti mengandung senyawa aktif sebagai senyawa pemandu dalam sintesis obat-obatan terbaru. Tujuan dari penelitian ini untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi spons Mycale vansoesti yang diperoleh dari perairan Pulau Mantehage terhadap bakteri Escherichia coli dan Staphylococcus aureus. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 95%. Pengujian Aktivitas Antibakteri menggunakan metode difusi agar Kirby and Bauer. Hasil penelitian menunjukkan bahwa spons Mycale vansoesti menghasilkan aktivitas antibakteri pada semua ekstrak dan fraksi yang digunakan yaitu ekstrak etanol, fraksi kloroform, fraksi n-heksan, dan fraksi metanol terhadap bakteri Escherichia coli dan Staphylococcus aureus dengan nilai sekitar 6,63-8,82 mm dan termasuk dalam daya hambat kategori sedang. Hal ini dibuktikan dengan terbentuknya zona bening disekitar cakram. Kata Kunci : Mycale vansoesti, Antibakteri, Staphylococcus aureus, Escherichia coli

scholarly journals Potensi Antimikroba Ekstrak Etanol Kulit Luar Buah Cempedak (Artocarpus integer (Thunb.) Merr.)

2019 ◽  
Vol 5 (1) ◽  
pp. 53-62
Author(s):  
Rina Saputri ◽  
Ali Rakhman Hakim ◽  
Dahlia Syahrina ◽  
Fatthiya Lisyanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Outer Skin ◽  
Biological Source ◽  
Activity Test ◽  
Escherichia Coli Bacteria ◽  
Mic Value

The background of this research is to develop the potential of biological resources in South Kalimantan. In particular, the biological source is cempedak fruit. The use of cempedak which has been scientifically studied is the use of cempedak bark as anti-malaria. Other studies related to other parts of cempedak are still not widely used. Based on the potential possessed by cempedak bark, it is likely that other parts of cempedak also have potential as traditional medicine. This study aims to identify the content of secondary metabolites from the outer skin of cempedak fruit, analyze the potential of the outer skin of cempedak fruit as an antimicrobial and analyze the effective dose as an antimicrobial. The method used to see MIC values with liquid dilution and KBM values with solid dilution. The results of phytochemical identification showed that the ethanol extract of the outer skin of cempedak fruit contained secondary metabolites in the form of saponins, tannins, flavonoids and alkaloids which have antimicrobial potential. The results of the antimicrobial activity test showed the MIC value of the ethanol extract of cempedak fruit outer skin against Escherichia coli bacteria at a concentration of 50% and against Staphylococcus aureus at a concentration of 25%. The KBM value also shows the same value as KHM. Based on the results of the study it can be concluded that the ethanol extract of cempedak fruit outer skin has broad-spectrum antimicrobial activity.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI ALGA Caulerpa racemosa DARI PERAIRAN PULAU MANTEHAGE MINAHASA UTARA TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 905
Author(s):  
Yosefa E.H. Ritan ◽  
Defny S. Wewengkang ◽  
Jainer Pasca Siampa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Chloroform Fraction ◽  
Caulerpa Racemosa ◽  
Gram Positive Bacteria ◽  
Chemical Content ◽  
Escherichia Coli Bacteria

ABSTRACTAlgae Caulerpa racemosa are able to produce chemical content that leave the potential to be antibacterial. The purpose of this study was to determine whether the extracts and fractions of algae Caulerpa racemosa had antibacterial activity against the Gram-negative bacteria Escherichia coli and the Gram-positive bacteria Staphylococcus aureus. The sample was extracted using the maceration method with 95% ethanol and the fractionation method used was liquid-liquid fractionation. Antibacterial activity testing was carried out using the disc diffusion agar method. The results obtained from the antibacterial activity test on Escherichia coli bacteria produce an inhibition zone in all extracts and fractions used while Staphylococcus aureus bacteria produced an inhibition zone for all  extracts and fractions, in ethanol extract with inhibitory value 6.72mm, n-hexane fraction 7.53mm, chloroform fraction 6.93mm and fraction methanol 7.32mm, respectively. The extracts and fractions of algae Caulerpa racemosa samples showed no activity against Escherichia coli, while Staphylococcus aureus has performed antibacterial activity with moderate category. The conclusion of this study, extracts and fractions of algae caulerpa racemosa had no antibacterial activity on the bacteria Escherichia coli while bacteria Staphylococcus aureus had antibacterial activity with moderate inhibition.Keywords: Algae Caulerpa racemosa, Antibacterial, Escherichia coli, Staphylococcus aureusABSTRAKAlga Caulerpa racemosa mampu menghasilkan kandungan kimia yang berpotensi sebagai antibakteri. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri  ekstrak dan fraksi dari alga Caulerpa racemosa di Perairan Pulau Mantehage Minahasa Utara terhadap bakteri Escherichia coli sebagai bakteri Gram-negatif dan Staphylococcus aureus sebagai bakteri Gram-positif. Sampel diekstraksi dengan menggunakan metode maserasi dengan pelarut etanol 95% dan metode fraksinasi yang digunakan yaitu fraksinasi cair-cair. Pengujian aktivitas antibakteri yang dilakukan menggunakan metode difusi agar. Hasil yang didapat dari uji aktivitas antibakteri pada bakteri Escherichia coli tidak menghasilkan zona hambat pada semua ekstrak dan fraksi yang digunakan sedangkan pada bakteri Staphylococcus aureus menghasilkan zona hambat pada semua ekstrak dan fraksi yaitu, ekstrak etanol dengan daya hambat 6,72mm, fraksi n-heksan 7,53mm, fraksi kloroform 6,93mm dan fraksi metanol 7,32mm. Kesimpulan dari penelitian ini, ekstrak dan fraksi dari sampel alga Caulerpa racemosa tidak memiliki aktivitas pada bakteri Escherichia coli sedangkan pada bakteri Staphylococcus aureus memiliki aktivitas antibakteri dengan daya hambat yang sedang.Kata Kunci: Alga Caulerpa racemosa, Antibakteri, Escherichia coli, staphylococcus aureus

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