Growth of Nannochloropsis oculata in shrimp cultivation waste at difference N:P ratios

The use of microalgae biomass in order to obtain lipids is an important alternative to be studied and it has great potential to be applied in order to produce food and biofuel, for instance. However, there are some processes of its production which need further study, such as the cultivation inputs. A possibility for an alternative raw material is the effluent from superintensive shrimp cultivation with bioflocs (BF). Therefore, the objective of this study was to evaluate the productivity and nutrient removal rate of Nannochloropsis oculata cultivation in three systems: (i) f/2 - produced integrally with chemical fertilizers, (ii) BF - using of 100% of the effluent for superintensive shrimp cultivation with bioflocs and (iii) 50/50 – using 50% of shrimp cultivation effluents and 50% from f/2 system. The microalgae presented greater biomass growth and productitvity in BF system but less lipids and esters accumulation. Concerning nutrient removal, f/2 system showed better performance, which may indicate that the cultivation in BF systems takes longer to reach the stationary growth phase.

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Efektifitas Ekstrak Nannochloropsis oculata Terhadap Peningkatan Kepadatan Kolagen pada Proses Penyembuhan Alveolar Osteitis

DENTA ◽

10.30649/denta.v10i1.16 ◽

2016 ◽

Vol 10 (1) ◽

pp. 9

Author(s):

Ajeng Saghita Enggardini ◽

Syamsulina Revianti ◽

Noengki Prameswari

Keyword(s):

Nannochloropsis Oculata ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Post Test ◽

Alveolar Osteitis

Latar Belakang: Alveolar osteitis merupakan salah satu komplikasi pasca pencabutan gigi. Kondisi ini biasanya menjadi masalah pasca pencabutan yang menyebabkan rasa nyeri dan menghambat penyembuhan soket. Ekstrak Nannochloropsis oculata mengandung alkaloid, carotenoid, flavonoid, terpenoid dan tocopherol yang dapat mempercepat proses penyembuhan luka. Tujuan: Mengetahui efektivitas ekstrak Nannochloropsis oculata terhadap peningkatan kepadatan kolagen pada proses penyembuhan alveolar osteitis. Bahan dan Metode: Rancangan penelitian ini adalah post test only control group design, dengan menggunakan 64 tikus Wistar jantan dibagi menjadi 2 kelompok besar, yaitu pemberian irigasi dan dressing. Pada masing-masing kelompok terdapat kelompok kontrol negatif (irigasi PBS atau dressing PBS), kelompok kontrol positif (irigasi Saline 0,9% atau dressing Alvogyl) dan kelompok perlakuan (irigasi atau dressing ekstrak Nannochloropsis oculata 2,5% dan 5%). Kemudian kepadatan kolagen diukur pada hari ke 3 dan ke 7 setelah perlakuan. Kepadatan kolagen yang diukur pada soket pasca pencabutan gigi. Setelah dilakukan treatment pada hari ke 3 dan ke 7, semua tikus dikorbankan lalu kepadatan kolagen diukur melalui preparat histopatologi anatomi dengan pengecatan Masson’s Trichom dan pembesaran 100x, kemudian dilakukan perhitungan kepadatan kolagen. Data yang diperoleh dianalisis Kruskal-Wallis dan Mann-Whitney. Hasil: Terdapat perbedaan kepadatan kolagen yang signifikan antara kelompok perlakuan irigasi (0,045), tetapi tidak terdapat perbedaan yang signifikan antara kelompok perlakuan dressing (0,243). Metode dressing lebih efektif dalam meningkatkan kepadatan kolagen jika dibandingkan dengan kelompok irigasi (0,026). Simpulan: Pemberian dressing ekstrak Nannochloropsis oculata konsentrasi 5% paling efektif dalam meningkatkan kepadatan kolagen pada proses penyembuhan alveolar osteitis pada hari ke 7.

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STUDY AND CHARACTERIZATION GROWTH OF FOUR MICROALGAE SPECIES AND TEST ANTIMICROBIAL ACTIVITY

Jurnal Zarah ◽

10.31629/zarah.v6i2.625 ◽

2018 ◽

Vol 6 (2) ◽

pp. 53-58

Author(s):

Marniati Salim

Keyword(s):

Dunaliella Salina ◽

Methanol Extract ◽

Sea Water ◽

Basal Medium ◽

Nannochloropsis Oculata ◽

Inhibitory Zone ◽

Antimicrobial Sensitivity ◽

Sensitivity Tests ◽

Microalgae Biomass ◽

Chaetoceros Calcitrans

Abstract In this study to the growth characteristics of microalgae (Dunaliella salina, Nannochloropsis oculata, Tetrasel mis chuii & Chaetoceros calcitrans), in different mediums, namely Bold’s Basal Medium (BBM) and BBM modification of sea water. The results obtained from microalgae (N.oculata, T.chuii, C.calcitrans) are better grown on BBM media while microlaga D.salina grows well on BBM modification of sea water. Microalgae biomass is extracted by maceration method in hexane and methanol solvents. Test bacteria used gram positive (Staphylococcus aureus) and gram negative (Escherichia coli). Inhibitory zone diameter results were compared with antimicrobial sensitivity tests according to Clinical and Laboratory Standards Institute (CLSI). From the results of microalgae hexane and methanol extract antibacterial activity tests (D.salina, N.oculata, T.chuii, C.calcitrans) showed that the extract was not too sensitive to inhibit bacterial growth. Keywords: microalgae, medium, antimicrobial

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Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.1.2.2013.7276 ◽

2013 ◽

Vol 1 (2) ◽

pp. 143

Author(s):

Petrus P Letsoin ◽

Henneke Pangkey ◽

Julius Sampekalo ◽

Inneke F.M Rumengan ◽

Stenly Wullur ◽

...

Keyword(s):

Body Size ◽

Marine Fish ◽

Fish Larvae ◽

Nannochloropsis Oculata ◽

Tropical Fish ◽

Sampling Location ◽

Larval Rearing ◽

Brachionus Rotundiformis ◽

Marine Fish Larvae ◽

Significant Difference

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae. Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.

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EFFECT OF SALINITY ON THE FATTY ACIDS COMPOSITION OF NANNOCHLOROPSIS OCULATA DURING EXPONENTIAL AND STATIONARY GROWTH PHASES

Egyptian Journal of Phycology ◽

10.21608/egyjs.2000.113237 ◽

2000 ◽

Vol 1 (1) ◽

pp. 151-155

Author(s):

Nagwa Mohammady

Keyword(s):

Fatty Acids ◽

Nannochloropsis Oculata ◽

Fatty Acids Composition ◽

Growth Phases ◽

Stationary Growth

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A photobioreactor using Nannochloropsis oculata marine microalgae for removal of polycyclic aromatic hydrocarbons and sorption of metals in produced water

Chemosphere ◽

10.1016/j.chemosphere.2021.130775 ◽

2021 ◽

pp. 130775

Author(s):

Isadora Machado Marques ◽

Adna Caroline Vale Oliveira ◽

Olivia Maria Cordeiro de Oliveira ◽

Emerson Andrade Sales ◽

Ícaro Thiago Andrade Moreira

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Aromatic Hydrocarbons ◽

Produced Water ◽

Nannochloropsis Oculata ◽

Marine Microalgae ◽

Polycyclic Aromatic

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Comparative study of tissue deposition of omega-3 fatty acids from polar-lipid rich oil of the microalgae Nannochloropsis oculata with krill oil in rats

Food & Function ◽

10.1039/c4fo00591k ◽

2015 ◽

Vol 6 (1) ◽

pp. 185-191 ◽

Cited By ~ 27

Author(s):

Michael L. Kagan ◽

Aharon Levy ◽

Alicia Leikin-Frenkel

Keyword(s):

Fatty Acids ◽

Comparative Study ◽

Polar Lipid ◽

Rat Plasma ◽

Nannochloropsis Oculata ◽

Polar Lipids ◽

Omega 3 Fatty Acids ◽

Krill Oil ◽

Omega 3 ◽

Epa And Dha

An oil from micro-algae rich in EPA with no DHA and consisting of 15% polar lipids (phospholipids and glycolipids) showed equivalent uptake of EPA into rat plasma and organs as omega-3 krill oil consisting of EPA and DHA and 40% phospholipids.

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Integrated mangrove-shrimp cultivation: Potential for blue carbon sequestration

AMBIO ◽

10.1007/s13280-017-0946-2 ◽

2017 ◽

Cited By ~ 4

Author(s):

Nesar Ahmed ◽

Shirley Thompson ◽

Marion Glaser

Keyword(s):

Carbon Sequestration ◽

Blue Carbon ◽

Shrimp Cultivation

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Macroalgae reveal nitrogen enrichment and elevated N:P ratios on the Belize Barrier Reef

Marine Pollution Bulletin ◽

10.1016/j.marpolbul.2021.112686 ◽

2021 ◽

Vol 171 ◽

pp. 112686

Author(s):

Brian E. Lapointe ◽

Alexander Tewfik ◽

Myles Phillips

Keyword(s):

Nitrogen Enrichment ◽

Barrier Reef ◽

N:P Ratios ◽

Belize Barrier Reef

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A co-cultivation process of Nannochloropsis oculata and Tisochrysis lutea induces morpho-physiological and biochemical variations potentially useful for biotechnological purposes

Journal of Applied Phycology ◽

10.1007/s10811-021-02511-2 ◽

2021 ◽

Author(s):

Michele Maglie ◽

Costanza Baldisserotto ◽

Alessandra Guerrini ◽

Alessandra Sabia ◽

Lorenzo Ferroni ◽

...

Keyword(s):

Lipid Production ◽

Maximum Yield ◽

Pharmaceutical Products ◽

Nannochloropsis Oculata ◽

Production Costs ◽

Yield Reduction ◽

Total Biomass ◽

Tisochrysis Lutea ◽

Positive Effects ◽

Cell Extracts

AbstractThe biotechnological potential of microalgae has gained considerable importance in many applied fields: biomass production for food and feed, cosmeceutical and pharmaceutical products, energy and phytoremediation. The driving force that inspires the progress in microalgae production is the need for new cultivation systems to obtain simultaneously the maximum yield, reduction of water and nutrients use, and production of economically interesting molecules, such as pigments, fatty acids and polysaccharides. We aim to test, for the first time, the co-cultivation in saline medium of Tisochrysis lutea (Haptophyta) and Nannochloropsis oculata (Ochrophyta) to obtain valuable compounds, i.e. pigments and lipids characteristic of each species, using a single culture process. Mono-cultures of each strain were used as controls. The two strains showed an increase in the concentration of chlorophylls and carotenoids in co-culture. At the end of the experiment, the fatty acid profile was analysed by gas chromatography–mass spectrometry. The lipids in the co-cultivated cell extracts were mainly attributable to N. oculata, which represented 97% of the total cells (ca. 83% of the total biomass) at the end of the experiment. Nevertheless, the ω-3 characteristic of T. lutea (DHA and SDA, absent in N. oculata) was also detectable. Although the co-cultivation of these two phylogenetically different species of microalgae did not show positive effects on the growth and on the total lipid production, however, this process resulted in a reduction of the production costs and a lower consumption of water and nutrients.

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