Effectiveness of plant essential oils derived from Curcuma longa, Illicium verum, Ocimum tenuiflorum, and Foeniculum vulgare for controlling common cutworm (Spodoptera litura)

Abstract Essential oils are well known to act as biopesticides. This research evaluated the acute toxicity and synergistic effect of essential oil compounds in combination with cypermethrin against Spodoptera litura Fabricius (Lepidoptera: Noctuidae). The effects of distillation extracts of essential oils from Alpinia galanga Zingiberaceae (Zingiberales) rhizomes and Ocimum basilicum Lamiaceae (Lamiales) leaves; one of their primary essential oil compounds 1,8-cineole; and linalool were studied on second-instar S. litura by topical application under laboratory conditions. The results showed that A. galanga had the highest control efficiency, whereas1,8-cineole provided a moderate efficacy. The mixtures of linalool, 1,8-cineole, O. basilicum, or A. galanga with cypermethrin were synergistic on mortality. Activity measurements of the main detoxification enzymes show that linalool and 1,8-cineole inhibit the activity of cytochromes P450 and carboxylesterases, which could explain their synergistic effect. Based on our results, the use of these mixtures represents an ideal eco-friendly approach, helping to manage cypermethrin resistance of S. litura.

Download Full-text

Testing of inhibition activity of essential oils against Paenibacillus larvae – the causative agent of American foulbrood

Acta Veterinaria Brno ◽

10.2754/avb201483010009 ◽

2014 ◽

Vol 83 (1) ◽

pp. 9-12 ◽

Cited By ~ 6

Author(s):

Katarína Kuzyšinová ◽

Dagmar Mudroňová ◽

Juraj Toporčák ◽

Radomíra Nemcová ◽

Ladislav Molnár ◽

...

Keyword(s):

Essential Oils ◽

Honey Bees ◽

Inhibitory Activity ◽

Paenibacillus Larvae ◽

Syzygium Aromaticum ◽

American Foulbrood ◽

Inhibition Activity ◽

Foeniculum Vulgare ◽

Plant Essential Oils ◽

Carum Carvi

American foulbrood is a dangerous world-wide spread disease of honey bees caused by the Paenibacillus larvae bacterium. Antibiotic treatments are less effective and leave residues in bee products. It is therefore necessary to find an alternative, especially using natural ingredients such as plant essential oils, probiotics, fatty or organic acids. Two strains of P. larvae were used for this study: CCM 4488, a strain from the Czech collection of micro-organisms and a Slovak field strain which was isolated from infected bee combs and characterized on the basis of biochemical properties. Plant essential oils of sage (Salvia officinalis), anise (Pimpinella anisum), oregano (Origanum vulgare), caraway (Carum carvi), thyme (Thymus vulgaris), rosemary (Rosmarinum officinalis), clove (Syzygium aromaticum), camomile (Chamomilla recutita) and fennel (Foeniculum vulgare) were used for the testing of the inhibitory activity against P. larvae. Essential oils at amounts of 5 µl and 10 µl were applied to sterile discs on MYPGP agar; inhibition zone diameters were measured after 24-h incubation at 37 °C. The strongest inhibitory activity against both P. larvae strains was noted in case of the essential oils from oregano, thyme and clove; essential oils from camomile, rosemary and fennel showed no or weak antibacterial activity. Medium strong inhibition activity was recorded in case of previously untested essential oil from Carum carvi. There was a difference in sensitivity of both tested strains to essential oils. Our study confirmed that some essential oils can be used in the prevention of American foulbrood but further experiments aimed at their influence on physiological intestinal microflora of honey bees must be performed.

Download Full-text

Antibiofilm and antioxidant activity of formulated nanoemulsion from Ocimum tenuiflorum

Proceedings International ◽

10.33263/proceedings21.007007 ◽

2020 ◽

Vol 2 (1) ◽

pp. 7

Keyword(s):

Essential Oils ◽

Textile Industry ◽

Mechanical Stability ◽

Immiscible Liquids ◽

Plant Essential Oils ◽

Pharmaceutical Ingredients ◽

Oil In Water ◽

Industry Applications ◽

Ocimum Tenuiflorum ◽

High Pressure Homogenizer

Nanoemulsions are nano-sized emulsions, which are made for improving the delivery of active pharmaceutical ingredients. These are the thermodynamically steady isotropic system where two immiscible liquids are mixed to form a single-phase by means of an emulsifying agent, i.e., surfactant and co-surfactant. In this study, the extracts of essential oils from medicinal plants, namely, Ocimum tenuiflorum (Tulasi) is used, which have antimicrobial, antioxidant, antibiofilm, and anti-inflammatory activities. Oil in water emulsions was prepared by high-pressure homogenizer using plant essential oils with water as a dispersion medium. The physical and mechanical stability tests were carried out. The emulsions were subjected to antibiofilm assays, and results were compared to estimate the anti-biofilm efficacy of the emulsions. The emulsions were prepared by varying concentrations and time to determine the particle size distribution. These results proposed for developed plant essential oil-based nanoemulsion is thermodynamically stable, and formulated nanoemulsion will be used in various biomedical applications, textile industry applications, and food industry.

Download Full-text

Ovicidal and Repellent Activities of Several Plant Essential Oils Against Periplaneta americana L. and Enhanced Activities from their Combined Formulation

10.21203/rs.3.rs-1091267/v1 ◽

2021 ◽

Author(s):

Mayura Soonwera ◽

Tanapoom Moungthipmalai ◽

Wacharaporn Takawirapat ◽

Sirawut Sittichok

Keyword(s):

Soybean Oil ◽

Essential Oils ◽

Ethyl Alcohol ◽

Eucalyptus Globulus ◽

Periplaneta Americana ◽

Repellent Activity ◽

Inhibition Rate ◽

Cymbopogon Citratus ◽

Plant Essential Oils ◽

Illicium Verum

Abstract Natural ovicidal and repellent agents against Periplaneta americana are in urgent need, and plant essential oils (EOs) can assume this role quite readily. In this study, ovicidal and repellent activities against Periplaneta americana of EOs from Cymbopogon citratus, Cinnamomum verum, Eucalyptus globulus, Illicium verum, and Zanthoxylum limonella in soybean oil and in ethyl alcohol were determined by topical and dual-choice assays, as well as 10% cypermethrin and a combined formulation of 5% C. verum EO + 5% I. verum EO. Cypermethrin at 10% provided the highest toxicity (100% inhibition rate) against the eggs, but only slightly higher than that (99.3%) provided by the combined EO formulation, while the highest repellent activity against the adults was provided by the combined formulation (89.5% repelled cockroaches at 48 h after treatment). In addition, all EO formulations in soybean oil provided higher ovicidal and repellent activities than in ethyl alcohol. To conclude, the combined EO formulation in soybean oil can replace cypermethrin because their efficacy was nearly equivalent, but the combination should be much safer to use.

Download Full-text

Synergistic Toxicity of Plant Essential Oils Combined with Pyrethroid Insecticides against Blow Flies and the House Fly

Insects ◽

10.3390/insects10060178 ◽

2019 ◽

Vol 10 (6) ◽

pp. 178 ◽

Cited By ~ 5

Author(s):

Suttida Suwannayod ◽

Kabkaew L. Sukontason ◽

Benjawan Pitasawat ◽

Anuluck Junkum ◽

Kwankamol Limsopatham ◽

...

Keyword(s):

Essential Oils ◽

Curcuma Longa ◽

Antagonistic Effect ◽

House Fly ◽

Gas Chromatography Mass Spectrometry ◽

Fruit Peel ◽

Pyrethroid Insecticides ◽

Blow Flies ◽

Plant Essential Oils ◽

Filth Flies

Blow flies (Diptera: Calliphoridae) and the house fly (Diptera: Muscidae) are filth flies of medical importance, and control of their population is needed. As insecticide applications have resulted in fly resistance, and the exploration of plant essential oils (EOs) has increased against filth flies, this study assessed the combination of EOs with pyrethoids to enhance toxic efficacy. The EOs of five effective plants were screened initially against the house fly (Musca domestica L.). Their chemical constituent was performed using gas chromatography-mass spectrometry (GC-MS) analysis. The main components of Boesenbergia rotunda (Zingiberaceae) rhizome, Curcuma longa (Zingiberaceae) rhizome, Citrus hystrix (Rutaceae) fruit peel, Ocimum gratissimum (Lamiaceae) seed, and Zanthoxylum limonella (Rutaceae) fruit were δ-3-caren (35.25%), β-turmerone (51.68%), β-pinene (26.56%), p-cumic aldehyde (58.21%), and dipentene (60.22%), respectively. The screening test revealed that the three most effective plant EOs were from B. rotunda, C. longa and O. gratissimum, which were selected for the combination with two pyrethroid insecticides (permethrin and deltamethrin), in order to enhance their synergistic efficacy against the blow flies, Chrysomya megacephala Fabricius, Chrysomya rufifacies Macquart, and Lucilia cuprina Wiedemann, and the house fly. Synergistic action was presented in almost all of the flies tested with permenthrin/deltamethrin/EOs mixtures. It was interesting that the combination of deltamethrin with three EOs showed a synergistic effect on all of the tested flies. However, an antagonistic effect was observed in C. megacephala and M. domestica treated with permethrin-B. rotunda and C. megacephala treated with permethrin-O. gratissimum. The LD50 of insecticides decreased when combined with plant EOs. This alternative strategy will be helpful in developing a formula for effective fly control management.

Download Full-text

Chemical Constituents and Combined Larvicidal Effects of Selected Essential Oils againstAnopheles cracens(Diptera: Culicidae)

Psyche A Journal of Entomology ◽

10.1155/2012/591616 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 5

Author(s):

Jitrawadee Intirach ◽

Anuluck Junkum ◽

Benjawan Tuetun ◽

Wej Choochote ◽

Udom Chaithong ◽

...

Keyword(s):

Essential Oils ◽

Synergistic Effects ◽

Chemical Constituents ◽

Curcuma Longa ◽

Larval Mortality ◽

Chemical Compositions ◽

Synergistic Activity ◽

Dependent Manner ◽

Foeniculum Vulgare ◽

Main Component

A preliminary study on larvicidal activity against laboratory-colonizedAnopheles cracensmosquitos revealed that five of ten plant oils at concentration of 100 ppm showed 95–100% larval mortality. The essential oils of five plants, includingPiper sarmentosum, Foeniculum vulgare, Curcuma longa, Myristica fragrans, andZanthoxylum piperitum,were then selected for chemical analysis, dose-response larvicidal experiments, and combination-based bioassays. Chemical compositions analyzed by gas chromatography coupled to mass spectrometry demonstrated that the main component in the oil derived fromP. sarmentosum, F. vulgare, C. longa, M. fragrans, and Z. piperitumwas croweacin (71.01%), anethole (63.00%), ar-turmerone (30.19%), safrole (46.60%), and 1,8-cineole (21.27%), respectively. For larvicidal bioassay, all five essential oils exerted promising efficacy in a dose-dependent manner and different performances onA. cracensafter 24 hours of exposure. The strongest larvicidal potential was established fromP. sarmentosum, followed byF. vulgare, C. longa, M. fragrans, andZ. piperitum, with LC50values of 16.03, 32.77, 33.61, 40.00, and 63.17 ppm, respectively. Binary mixtures betweenP. sarmentosum, the most effective oil, and the others at the highest ratio were proved to be highly efficacious with a cotoxicity coefficient value greater than 100, indicating synergistic activity. Results of mixed formulations of different essential oils generating synergistic effects may prove helpful in developing effective, economical, and ecofriendly larvicides, as favorable alternatives for mosquito management.

Download Full-text

THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2018.6.17 ◽

2018 ◽

Vol 17 (6) ◽

pp. 167-174 ◽

Cited By ~ 1

Author(s):

Małgorzata Schollenberger ◽

Tomasz M. Staniek ◽

Elżbieta Paduch-Cichal ◽

Beata Dasiewicz ◽

Agnieszka Gadomska-Gajadhur ◽

...

Keyword(s):

Essential Oils ◽

Pseudomonas Syringae ◽

Pathogenic Bacteria ◽

Antimicrobial Effect ◽

Mentha Piperita ◽

Mentha Spicata ◽

Plant Essential Oils ◽

Plant Pathogenic Bacteria ◽

Interspecies Hybrids

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

Download Full-text

Foeniculum vulgare Essential Oils: Chemical Composition, Antioxidant and Antimicrobial Activities

Natural Product Communications ◽

10.1177/1934578x1000500231 ◽

2010 ◽

Vol 5 (2) ◽

pp. 1934578X1000500 ◽

Cited By ~ 15

Author(s):

Maria Graça Miguel ◽

Cláudia Cruz ◽

Leonor Faleiro ◽

Mariana T. F. Simões ◽

Ana Cristina Figueiredo ◽

...

Keyword(s):

Essential Oils ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Point Of View ◽

Diffusion Method ◽

Foeniculum Vulgare ◽

Aerial Parts ◽

Scavenging Capacity ◽

Dpph Method ◽

Main Components

The essential oils from Foeniculum vulgare commercial aerial parts and fruits were isolated by hydrodistillation, with different distillation times (30 min, 1 h, 2 h and 3 h), and analyzed by GC and GC-MS. The antioxidant ability was estimated using four distinct methods. Antibacterial activity was determined by the agar diffusion method. Remarkable differences, and worrying from the quality and safety point of view, were detected in the essential oils. trans-Anethole (31-36%), α-pinene (14-20%) and limonene (11-13%) were the main components of the essentials oil isolated from F. vulgare dried aerial parts, whereas methyl chavicol (= estragole) (79-88%) was dominant in the fruit oils. With the DPPH method the plant oils showed better antioxidant activity than the fruits oils. With the TBARS method and at higher concentrations, fennel essential oils showed a pro-oxidant activity. None of the oils showed a hydroxyl radical scavenging capacity >50%, but they showed an ability to inhibit 5-lipoxygenase. The essential oils showed a very low antimicrobial activity. In general, the essential oils isolated during 2 h were as effective, from the biological activity point of view, as those isolated during 3 h.

Download Full-text