scholarly journals The effect of light and gibberellic acid concentrations on breaking dormancy of potato micro tuber

2021 ◽  
Vol 883 (1) ◽  
pp. 012011
Author(s):  
A K Karjadi ◽  
N Waluyo
Keyword(s):  
Block Design ◽  
Storage Conditions ◽  
Visual Observation ◽  
Potato Tubers ◽  
Randomized Block Design ◽  
Breaking Dormancy ◽  
Shoot Number ◽  
And Storage ◽  
Effect Of Light

Abstract Potato (Solanum tuberosum L) is one of Indonesia’s priority crops considering its nutritive benefit as a carbohydrates source in food diversification. Potato tubers are classified as stem tubers, so the tubers such as micro tuber can appear in the stem of in vitro cuttings. The research aimed to determine the effect of GA3 concentrations (0, 5, 10, 15, 20 ppm) and storage conditions, e.g., Dark (T1) and light (T2), on breaking dormancy of potato micro tubers. This experiment was conducted in the Tissue culture Laboratory of IVEGRI from April until September 2018. The experiment used a randomized block design with 3 replications; each treatment consisted of 40 micro tubers of Granola (var.) with size ranged >3 - <4 g per each. The result showed that storage conditions gave significant differences to the percentage of breaking dormancy for 4-6 weeks, and GA3 concentration did not affect the average number of shoot, average shoot length in concentration 5 until 20 ppm. The average shoot number, shoot lengths were 0.63 – 2.20 per tubers, 7.50 – 20.13 mm, respectively. There was no interaction between treatment. Visual observation concluded that storage in light conditions produced shoots with better quality than ones in dark conditions.

scholarly journals Nutritional value of sorghum silages

2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Significance Level ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
Nutritional Characteristics ◽  
High Participation ◽  
Sorghum Genotypes

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 44
Author(s):  
Hanisa Desy Ariani ◽  
Noor Aidawati ◽  
Dewi Arika Adriani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhizoctonia Solani ◽  
Plant Protection ◽  
Block Design ◽  
Sheath Blight ◽  
In Vitro Test ◽  
Sheath Blight Disease ◽  
Randomized Block Design ◽  
Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

scholarly journals The Effect of Processing and Cooling Methods on Coleus tuberosus in vitro Starch Digestibility

2021 ◽  
Vol 41 (3) ◽  
pp. 238
Author(s):  
Jhauharotul Muchlisyiyah ◽  
Tri Dewanti Widyaningsih ◽  
Retno Wulansari ◽  
Hera Sisca Prasmita
Keyword(s):  
Resistant Starch ◽  
Room Temperature ◽  
Potato Starch ◽  
Block Design ◽  
In Vitro Digestibility ◽  
Randomized Block Design ◽  
Two Factors ◽  
Cooling Methods ◽  
Total Starch

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.

Pemanfaatan Hasil Samping Fermentasi Belimbing Wuluh (Asam Sunti) dalam Pengolahan Ikan

2018 ◽  
Vol 3 (2) ◽  
pp. 351-356
Author(s):  
Weny Weny ◽  
Rasdiansyah Rasdiansyah ◽  
Novia Mehra Erfiza
Keyword(s):  
Soluble Protein ◽  
Room Temperature ◽  
Storage Time ◽  
Ph Value ◽  
Block Design ◽  
Total Acid ◽  
Soaking Time ◽  
Ph Values ◽  
Randomized Block Design ◽  
And Storage

Abstrak. Sampai saat ini belum ada penelitian lebih lanjut mengenai pemanfaatan hasil samping dari produk asam sunti. Tujuan penelitian ini untuk melihat pengaruh perendaman ikan di dalam air asam sunti dan penyimpanannya pada suhu ruang terhadap karakteristik ikan. Penelitian ini menggunakan Rancangan Acak Kelompok Faktorial dengan 2 faktor yaitu faktor waktu perendaman (W), yang terdiri dari 3 taraf (W1 : 30 detik, W2 : 5 menit, W3 : 10 menit) dan lama penyimpanan (S), yang terdiri dari 2 taraf (S1 : 0 jam, S2 : 2 jam). Hasil penelitian menunjukkan bahwa karakteristik air asam sunti pada uji pH memiliki rataan yaitu 1,27, total asam 49% dan total bakteri asam laktat  5,1×104 CFU/gram. Nilai pH ikan setelah direndam air asam sunti 5,22-5,65 dengan rataan 5,43 dan nilai protein terlarut berkisar antara 18,42-18,74% dengan rataan 18,74%.Dari penelitian didapatkan bahwa waktu perendaman (W) berpengaruh nyata (P≤0,05) terhadap nilai protein terlarut dari ikan namun berpengaruh tidak nyata (P0,05) terhadap nilai pH ikan, uji deskriptif aroma dan tekstur ikan. Lama penyimpanan (S) berpengaruh sangat nyata (P≤0,01) terhadap nilai pH ikan setelah perendaman namun berpengaruh tidak nyata (P0,05) terhadap uji deskriptif aroma dan tekstur ikan. Interaksi waktu perendaman dengan lama penyimpanan berpengaruh nyata (P≤0,05) terhadap nilai pH ikan namun berpengaruh tidak nyata terhadap uji deskriptif aroma dan tekstur ikan.Waktu perendaman ikan di dalam air asam sunti selama 10 menit dapat menghambat kenaikan pH ikan selama penyimpanan sampai 2 jam. Semakin lama perendaman ikan di dalam air asam sunti maka semakin tinggi protein terlarut dari ikan.  Abstrack. Therefore, this study was conducted to see the effect of soaking the fish in asam sunti water and its storage at room temperature on the characteristics of the fish. This study uses a randomized block design factorial with 2 factors: soaking time (W), consisting of 3 levels (W1: 30 seconds, W2: 5 minutes, W3: 10 minutes) and storage time (S), consisting of 2 levels ( S1: 0 hours, S2: 2 hours). The results showed pHof asam sunti water is 1.27,  total acid is 49% and lactic acid bacteria has  of  5.1 × 104 CFU / gram and the soaked fish has pH values ranged from 5.22 to 5.65 with the average of 5.43 and soluble protein values ranged from 18.42 to 18.74% with the average 18.74%.The results indicated that the soaking time (W) has effect significantly to the value of soluble protein (P≤0,05) of fish but iteffect not significantly (P 0.05) on pH value of fish, descriptive test the of aroma and texture of the fish. Storage time (S) effect significantly (P≤0,01) to pH value of fish after soaking but it effectnot significantly (P 0.05) to the descriptive test of  aroma and texture of the fish. Interaction withsoaking time and storage (WxS) effect significantly (P≤0,05) to pH value of fish but it effect to not significantly the descriptive test of aroma and texture of the fish.Soaking time the fish in water for 10 minutes sunti can inhibit increasing in the pH of the fish during storage up to 2 hours. The longer soaking time of the fish in the asam sunti water, the higher sunti acid soluble proteins from value.

scholarly journals POTENTIAL OF PLANT EXTRACTS AND FUNGICIDES FOR MANAGING FUSARIUM OXYSPORUM F. SPLYCOPERSICI

2018 ◽  
Vol 30 (1) ◽  
pp. 75 ◽  
Author(s):  
Gul B. Poussio ◽  
Manzoor A. Abro ◽  
Jamal U. D. Hajano ◽  
Muhammad I. Khaskheli ◽  
Khalid I. Rajput ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Mycelial Growth ◽  
Disease Incidence ◽  
Block Design ◽  
Fungal Growth ◽  
Tomato Crop ◽  
Randomized Block Design ◽  
Sindh Province ◽  
Causal Fungus

Fusarium oxysporum f.sp. lycopersici (FOL) is a highly destructive fungal pathogen of tomato crop causing wilt disease which may reduce 10 to 90% yield. In Pakistan, tomato is widely grown in Sindh province, major territories are district Hyderabad, Tando Allahyar and Mirpurkhas. Thus, surveys of these territories were conducted to record intensity of the disease and confirm etiology. Furthermore, potential of different botanical pesticides and commercially available fungicides were tested to inhibit mycelial growth of the causal fungus. The experiment was laid down with complete randomized block design with three replications. The results showed that the disease was occurring in all locations with the range of 8-47 % incidence. F. oxysporum f.sp. lycopersici was predominantly isolated from the collected disease sample during survey and pathogenic nature of the fungus was tested on the tomato Golo variety through soil drenching method. The disease incidence of 30 and 42 % (72 % as total) was recorded in inoculated tomato plants at 20 and 40 DAI, respectively. Maximum (67 %) inhibition of the fungal growth was found by neem seed extracts at higher dose of 8 % concentration followed by 63 % with neem seeds and Eucalyptus at 6 and 8 %, respectively. Alternatively, the Nativo 75 WG fungicide was found most effective in reducing the redial mycelial growth of target fungus followed by Topsin-M at 1000 ppm where as Aliette and Melodedue fungicides were found least effective under in-vitro conditions.

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