scholarly journals Bovine semen thermoresistance tests and their correlation with pregnancy rates after fixed-time artificial insemination

2019 ◽  
Vol 71 (6) ◽  
pp. 2085-2092
Author(s):  
R. Talini ◽  
L.E. Kozicki ◽  
F.R. Gaievski ◽  
G. Polo ◽  
L.G.F. Lima ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Sperm Motility ◽  
Negative Correlation ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Rapid Test ◽  
Significant Negative Correlation ◽  
Fixed Time ◽  
Bovine Semen ◽  
The Mean

ABSTRACT The objective of the study was to determine whether or not there is a correlation between thermoresistance tests (TT) after semen thawing and pregnancy rate (PR) after fixed-time artificial insemination (FTAI). Four different TT were performed on ten samples used for AI; a rapid test (RTT) (30min / 46°C) and three slow tests (STT): STT1 (60min/38°C), STT2 (180min/38°C), and STT3 (300min/38°C). Two hundred and fifteen multiparous crossbred cows were submitted to FTAI under the following protocol: on day zero (d0) the animals received a P4 device +EB; on d7 PGF2α; on d8 P4 was removed and eCG+EC were administered; IATF was performed on d10. Three gestational diagnoses (G D) were performed on d40, d70 and d120. The mean sperm motility (%) in RTT and STTs were 19.84±6.13, 28.55±10.48, 17.62±5.87 and 8.63±3.46, respectively, and TP in the three DG 61.86%, 57.67%, and 55.81%, respectively. Through Person test a significant negative correlation (P< 0.05) was found between STT2 and PR at 60 days (r= -0.644) and between STT3 and all TPs (r= -0.774, -0.752, 0.748). It was concluded that TT parameters are not able to determine correlation between semen quality and TP.

ASSESSMENT OF BOVINE SEMEN QUALITY BY RESAZURIN REDUCTION TEST

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Umesh Kumar ◽  
Prasanna Pati ◽  
A.P. Gawande
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Mean Value ◽  
Bovine Semen ◽  
Reduction Test ◽  
The Mean ◽  
Acrosomal Integrity

A study was conducted to assess the semen quality using Resazurin Reduction Test (RRT) in Jersey and Jersey cross bulls. A total 36 fresh ejaculates were collected for evaluation RRT, sperm motility, acrosomal integrity and sperm concentration. The mean value of RRT in bulls was 15.77±0.85 minutes.Statistical analysis revealed that RRT was highly significantly (p<0.01) and positively correlated with sperm concentration (r=0.769), sperm motility(r=0.858) and acrosomal integrity (r=0.515), hence it may be helpful in evaluating the quality of semen in bulls.

scholarly journals Relationship between viability of thawed semen and pregnancy rate of Nelore cows subjected to fixed-time artificial insemination

2021 ◽  
Vol 10 (2) ◽  
pp. e49710212184
Author(s):  
Fernando Roberto Pereira ◽  
Ana Beatriz Marques de Almeida ◽  
Myrian Megumy Tsunokawa Hidalgo ◽  
José Roberto Potiens ◽  
Anne Kemmer Souza ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Membrane Integrity ◽  
Fixed Time ◽  
Mitochondrial Activity ◽  
Bovine Semen ◽  
Conventional Microscopy ◽  
Integrity Analysis ◽  
The Relationship

This research aimed to evaluate the relationship of membrane integrity analysis, mitochondrial cytochemical analysis, and sperm kinetics of bovine semen after thawing and the pregnancy rate in Nelore females submitted to fixed-time artificial insemination (FTAI). Thirty-seven conventional semen straws, from 16 Nelore bulls and 3 Aberdeen Angus bulls were used. The semen samples were evaluated in conventional microscopy for sperm motility, vigor, and morphology, through flow cytometry for membrane integrity and mitochondrial function, and the CASA system for evaluation of sperm kinetics. All females were subjected to the same FTAI protocol and were inseminated by the same technical team. The correlation between all variables was performed by Pearson's correlation. Cluster analysis was used to analyze the relationship between pregnancy rate and combinations of laboratory tests (P ≤ 0.05). Semen samples from Angus bulls (89.2 ± 5.5) presented superior VSL (P=0.004) to Nelore semen (78.6 ± 8.6), but this did not affect the pregnancy rate (Nelore: 51.0% versus Angus: 50.5%, P > 0.05). By flow cytometry, 44.3% membrane integrity and 47.3% mitochondrial integrity were identified. Five clusters were selected, one of which presented the best pregnancy rate, containing the most balanced rates between progressive velocity and mitochondrial activity (VSL 71.16 μm/s, MITO 49.69%, TxP 62.4%). Samples of thawed bovine semen with progressive velocity and balanced mitochondrial activity demonstrated better fertility to TAI program.

Cooled semen for fixed-time artificial insemination in beef cattle

2016 ◽  
Vol 28 (7) ◽  
pp. 1004 ◽  
Author(s):  
Juliana C. Borges-Silva ◽  
Márcio R. Silva ◽  
Daniel B. Marinho ◽  
Eriklis Nogueira ◽  
Deiler C. Sampaio ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Egg Yolk ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Reproductive Efficiency ◽  
Sperm Abnormalities ◽  
Hypoosmotic Swelling Test ◽  
Interesting Approach

This study evaluated the use of cooled semen in a fixed-time artificial insemination (FTAI) program compared with frozen–thawed semen to improve pregnancy rates in beef cattle. Ejaculates of three bulls were collected and divided into two treatments: (1) frozen–thawed semen and (2) cooled semen. Egg-yolk extender without glycerol was used for the cooled semen treatment. Straws (25 × 106 spermatozoa) were submitted to cooling for preservation at 5°C for 24 h, after which FTAI was performed. Nelore cows (n = 838) submitted to FTAI were randomly inseminated using frozen–thawed semen or cooled semen. There was a 20% increase in the pregnancy per AI (P AI–1) using cooled semen compared with frozen–thawed semen (59.9 ± 4.7 vs 49.4 ± 5.0%; P < 0.005). There was no difference in P AI–1 among the bulls (P = 0.40). The frozen–thawed semen had fewer functional spermatozoa than did the cooled semen when evaluated by sperm motility (61.7 vs 81.0%), slow thermoresistance test (41.7 vs 66.7%) and hypoosmotic swelling test (38.3 vs 53.7%; P < 0.05). The percentage of sperm abnormalities did not differ between the freeze–thawing and cooling processes (18.6 vs 22.1%; P > 0.05). Because there was less damage to spermatozoa and improvement in P AI–1, the use of cooled semen instead of frozen–thawed semen is an interesting approach to increase reproductive efficiency in cattle submitted to a FTAI protocol.

Efficiency of mating, artificial insemination or resynchronisation at different times after first timed artificial insemination in postpartum Nellore cows to produce crossbred calves

2019 ◽  
Vol 59 (2) ◽  
pp. 225 ◽  
Author(s):  
Walvonvitis Baes Rodrigues ◽  
Jean do Prado Jara ◽  
Juliana Correa Borges ◽  
Luiz Orcirio Fialho de Oliveira ◽  
Urbano Pinto Gomes de Abreu ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Beef Cows ◽  
Device Removal ◽  
Oestradiol Benzoate ◽  
Timed Artificial Insemination ◽  
Equine Chorionic Gonadotropin

The objective of this trial was to evaluate different post-timed artificial insemination (TAI) reproductive managements in postpartum beef cows to produce crossbred calves from artificial insemination (AI). Nellore cows (n = 607), with 45 days postpartum, were inseminated at a fixed time, using a protocol that included an intravaginal progesterone-releasing device along with oestradiol benzoate, prostaglandin, equine chorionic gonadotropin, and oestradiol cypionate, followed TAI 48 h post-device removal. Four post-TAI treatments were evaluated: in CONTROL (T1, n = 161), cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). In OBSERVATION (T2, n = 132), heat detection was performed for 15–25 days post-TAI, followed by AI. In RESYNC22 (T3, n = 157) and RESYNC30 (T4, n = 157), resynchronisation started after 22 or 30 days, following second TAI at Day 32 or 40 days after first TAI. In T2, T3 and T4, after the second AI, cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). The pregnancy rate (PR) for the first TAI did not differ (54.6%, 53.0%, 59.2%, and 51.6% for CONTROL, OBSERVATION, RESYNC 22, and RESYNC 30, respectively; P = 0.66), and no difference was observed for the second TAI (RESYNC 22 = 45.31% and RESYNC30 = 46.05%; P = 0.137), in the PR at the end of the breeding season (86.33%, 86.36%, 78.98%, and 81.52%, P = 0.43), or embryonic losses (4.54%, 2.85%, 6.45% and 7.40%, respectively; P = 0.61), but the percentage of crossbred pregnancy was higher in groups with resynchronisation (RESYNC22 and RESYNC30) than CONTROL and OBSERVATION (98.38%, 90.62%, 63.30%, 78.95%, P &lt; 0.001). In conclusion, resynchronisation programs of 22 or 30 days are more efficient to produce AI products, and the final pregnancy rate is similar among the treatments, differing only in the amount of calves produced by AI.

scholarly journals Effect of progesterone, human chorionic gonadotropin and progesterone + human chorionic gonadotropin treatment on conception rates in repeat breeder cows

2020 ◽  
Vol 89 (4) ◽  
pp. 307-315
Author(s):  
Hasan Alkan ◽  
Huseyin Erdem
Keyword(s):  
Pregnancy Rate ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Group 4 ◽  
Prostaglandin F ◽  
Repeat Breeder ◽  
Group 1

The aim of this study was to investigate the effects of hormonal support on the pregnancy rate in repeat breeder cows. Prostaglandin F2α + Ovsynch oestrus synchronization protocol was applied to the cows. Following the fixed time insemination (day 0), the cows were divided into 4 groups. In Group 1 (n = 42), progesterone releasing intravaginal device (PRID) was placed vaginally at 84 h and removed on the 9th day after the artificial insemination. In Group 2 (n = 40), the cows were administered human chorionic gonadotropin (hCG) on the 7th day. Group 3 (n = 45) was applied a combination of progesterone and hCG. Group 4 (n = 42) was not given any treatment. Blood samples were collected from all cows 4 times on days 3.5, 7, 12, and 18 to evaluate progesterone concentrations. The pregnancy rates were 40.47%, 37.50%, 44.44%, and 30.95% in Group 1, 2, 3, and 4, respectively (P > 0.05). In addition, in cows with progesterone concentrations <2 ng/ml on day 3.5, the pregnancy rates were found to be lower than in the cows with progesterone concentrations >2 ng/ml in Group 4 (P < 0.05). Progesterone supplementation in cows with progesterone concentrations < 2 ng/ml appeared to increase pregnancy rates (P < 0.05) in Groups 1 and 3. As a result, post-insemination hormonal applications in the repeat breeder cows did not increase the pregnancy rate. However, it was concluded that determination of progesterone concentrations on day 3.5 following artificial insemination and then hormonal support in the cows with low concentrations would increased the pregnancy rate.

Chemical prediction of water activity in processed cheese

1989 ◽  
Vol 56 (4) ◽  
pp. 665-668 ◽  
Author(s):  
María A. Esteban ◽  
Andrés Marcos
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Water Activity ◽  
Negative Correlation ◽  
Linear Regression Analysis ◽  
Significant Negative Correlation ◽  
Regression Equation ◽  
Low Fat ◽  
Two Samples ◽  
The Mean

SummaryBy linear regression analysis, a highly significant negative correlation (r = −0·96) was found between the mean ash concentration values (g/100 g moisture) and water activity (aw) of six types of processed cheeses (low-fat, semi-fat, fat, extra-fat, double fat and special). The regression equation aw = 0·9951 − 0·0032* (ash), applied to 40 cheese samples, yielded aw values which differed by < 0·005 aw units from those measured experimentally in 75% of the samples. The maximum differences between the calculated and experimental aw values (found in only two samples) were ±0·01 aw units.

23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

2020 ◽  
Vol 32 (2) ◽  
pp. 137
Author(s):  
Y. Pirosanto ◽  
M. Valera ◽  
A. Molina ◽  
J. Dorado ◽  
S. Demyda-Peyrás
Keyword(s):  
Sperm Motility ◽  
Inbreeding Depression ◽  
Negative Correlation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Inbreeding Coefficient ◽  
Semen Parameters ◽  
Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P&lt;0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P&lt;0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P&lt;0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P&gt;0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

scholarly journals Effect of soybean plant phenols and flavonoid on the mean leaf area consumed by Spodopteralitura and Spilosoma obliqua larvae

2016 ◽  
Vol 8 (4) ◽  
pp. 1931-1936
Author(s):  
Anchala Nautiyal ◽  
Neeta Gaur ◽  
Kamendra Singh ◽  
Preeti Sharma
Keyword(s):  
Leaf Area ◽  
Negative Correlation ◽  
Phenolic Content ◽  
Significant Negative Correlation ◽  
Correlation Study ◽  
Soybean Plant ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Plant Phenols ◽  
The Mean

The aim of the present investigation was to study the effect of soybean plant phenols and flavonoid content on the mean leaf area consumed by Spodopteralitura and Spilosoma obliqua larva. Phenols and flavonoid content in methanolic leaf extract of thirty three genotypes of soybean were determined by spectrophotometrically. Thehighest and lowest phenolic content were found in genotypes JS-20-41(2.2±0.073 mg/g) and CSB 904 (0.45 ±0.11 mg/g), respectively. While the highest and lowest flavonoid content was found in genotypes SL 979 4.686± 0.062 mg QE/ g, respectively. In correlation study a highly significant negative correlation was observed between mean leaf area consumed (cm2) by S. litura, phenol content (-0.741 ) and flavonoid content (-0.737) similarly a highly significant negative correlation was observed between mean leaf area consumed by S. obliqua, phenol content (-0.728) and flavonoid content (-0.736) in leaves. Hence it can be concluded that, the genotypes which were having higher phenol and flavonoid content in their leaves offered resistance against S. litura and S. boliqua in soybean.

Sign in / Sign up

Export Citation Format

Share Document