scholarly journals Potential and phylogenetic of superior bacterial isolates in biogas sludge from anaerobic digestion of palm oil mill effluent

2021 ◽  
Vol 913 (1) ◽  
pp. 012065
Author(s):  
N E Mustamu ◽  
Z Nasution ◽  
Irvan ◽  
M Sembiring
Keyword(s):  
Anaerobic Digestion ◽  
Bacterial Isolates ◽  
Total N ◽  
Randomized Design ◽  
Sequencing Method ◽  
Rrna Sequencing ◽  
Palm Oil Mill ◽  
Completely Randomized Design ◽  
Phosphate Solubilizing ◽  
Potential Test

Abstract The study obtains potential and identifies Superior Bacterial Isolates (SBI), i.e., nitrogen-fixing and phosphate solubilizing from biogas sludge. The potential test was conducted using a Completely Randomized Design (CRD) within three replications, and the means were determined by ANOVA and DMRT at Р< 0.05. The molecular identification of SBI is used by the PCR-16S rRNA sequencing method. This study was conducted from January to August 2020. The result found in the N3 and P7 from biogas sludge was more potential in the availability of total-N and available-P compared to other isolates. It also was identified as similar to Bacillus paramycoides and Bacillus cereus, respectively. This information can be used as a reference that biogas sludge can be used to support soil fertility.

scholarly journals Solubilization of Araxá natural phosphate and decomposition of plant residues by bacterial isolates

2020 ◽  
Vol 44 ◽  
Author(s):  
Mário Viana Paredes Filho ◽  
Adriano Bortolotti da Silva ◽  
Ligiane Aparecida Florentino
Keyword(s):  
Culture Medium ◽  
Plant Residues ◽  
Market Demand ◽  
Bacterial Isolates ◽  
Natural Phosphate ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Phosphate Solubilizing ◽  
Different Sources ◽  
Decomposition Of Plant Residues

ABSTRACT Brazil imports most of the phosphate fertilizers used in agriculture, however, it has national deposits in its territory that can supply part of the domestic market demand. In order of these to be used effectively, it is necessary to identify techniques to accelerate the release of phosphorus (P) from these rocks. The objective was to evaluate the influence of inoculation with phosphate solubilizing bacterial isolates (PSB) and decomposition of different plant residues in the solubilization of Araxá natural phosphate (ANP). Two experiments were carried out in a completely randomized design in a 5 x 4 factorial scheme, with four replicates. The first experiment evaluated the solubilization of ANP by PSB in culture medium with four different sources of carbon: glucose, saccharose, cellulose, and pectin. The treatments were incubated for seven days and then were analyzed the P concentration and pH values. In the second experiment, the ANP was applied in 300 cm3 of soil, placed in incubation chambers, with six grams of dry and crushed plant material of white oat, brachiaria, crotalaria, lupine, and the inoculation of PSB. Decomposition was evaluated weekly by quantifying the carbon of the CO2-C released and, after 42 days was analyzed the concentration of P in the soil. It was observed that the carbon source directly influences the solubilization of ANP by PSB, especially the genus Burkholderia. Treatments containing crotalaria and lupine residues showed higher CO2-C values, and the ones containing plant residues associated with BSF inoculation obtained the highest levels of P in the soil.

scholarly journals Effect of biofertilizer obtained by anaerobic digestion of cassava effluent on the development of crambe plants

2017 ◽  
Vol 21 (10) ◽  
pp. 681-685 ◽  
Author(s):  
Andressa C. Neves ◽  
Camila N. Bergamini ◽  
Rafaela de O. Leonardo ◽  
Manoel P. Gonçalves ◽  
Dilcemara C. Zenatti ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Plant Development ◽  
Root Biomass ◽  
Oil Content ◽  
Final Height ◽  
Control Treatment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Shoot And Root Biomass ◽  
Number Of Branches

ABSTRACT This study aimed to evaluate the effect of applying increasing doses of biofertilizer obtained by the anaerobic digestion of cassava effluent on the development of crambe plants. The experiment was conducted in a protected environment at the Federal University of Paraná (UFPR), Palotina Sector, between April and August 2015. A completely randomized design was used, and five different treatments with the following doses were applied in five replicates: 0, 40, 80, 120, and 160 kg ha-1 of K2O. The following parameters related to plant development were evaluated: final height, stem diameter, number of branches, dry shoot and root biomass, mass of the grains, and oil content. The 160 kg K2O ha-1 dose was found to have the best influence on the plant development, because all the measured parameters reached their highest values at this dose, except for oil content, which attained the highest percentage in the case of the control treatment (0 kg ha-1 of K2O). This study proved that the biofertilizer obtained by anaerobic digestion of cassava effluent can be used as an alternative to regular fertilizers in cultivating crambe.

scholarly journals FERMENTATION OF WHEY WASTE AS ORGANIC LIQUID FERTILIZER "PUCAFU"

2017 ◽  
Vol 2 (2) ◽  
pp. 7-13
Author(s):  
Muhammad Akhsan Akib ◽  
Henny Setiawati
Keyword(s):  
Organic Liquid ◽  
Total N ◽  
Organic C ◽  
Fermentation Time ◽  
Liquid Fertilizer ◽  
Randomized Design ◽  
Two Factors ◽  
Liquid Wastes ◽  
Completely Randomized Design ◽  
Yeast Concentration

Whey waste contains organic materials, particularly high complex proteins and amino acids in the form of suspended and dissolved solids, however the utilization of whey as a organic liquid fertilizer still has a less attention. Thus the Utilization of the whey waste through anaerobic process to be used as a organic liquid fertilizer is the purpose of the research. This research was conducted using factorial design with completely randomized design (CRD) which consists of two factors: the yeast concentration (without yeast; 0.25 and 0.50 g/500 ml of whey waste) and the fermentation time (0, 3, and 5 days). The variables measured were the content of organic C, C/N Ratio, and Total N, P2O5 and K2O contents. The results showed that the fermented whey waste on the different fermentation time and yeast concentration had increased the organic C and C/N ratio, but decreased P2O5 and K2O contents. The utilization of whey combined with solid or other liquid wastes gave a chance to produce a quality organic liquid fertilizer 

Effect of Nanoparticle Phosphate Rock and Phosphate Solubilizing Fungi on Soil P-Potential, P-Retention, Organic Carbon and Base Saturation on Cilembu’s Inceptisols

2021 ◽  
Vol 1044 ◽  
pp. 143-150
Author(s):  
Muhammad Amir Solihin ◽  
Pujawati Suryatmana ◽  
Fajri Syahid Nurhakim ◽  
Rina Devnita ◽  
Mahfud Arifin
Keyword(s):  
Soil Properties ◽  
Crop Production ◽  
Phosphate Rock ◽  
Base Saturation ◽  
Organic P ◽  
Soil P ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Phosphate Solubilizing ◽  
P Retention

Intensive agricultural cultivation on Cilembu’s Inceptisols has become several soil properties problem for crop production. Nanoparticle phosphate rock and phosphate solubilizing fungi have ability to increase some soil properties content. The research aimed to observe the effect of nanoparticle phosphate rock and phosphate solubilizing fungi on soil P-potential, P-retention, C-organic and base saturation on Cilembu’s Inceptisols. The experiment arranged in Completely Randomized Design. The nanoparticle phosphate rock consisted of 4 levels. The phosphate solubilizing fungi consisted of 2 levels, and 2 replication. Soil P-Potential, P-Retention, C-organic, and Base Saturation were observed. The factors was observed after one month incubation on soil. Results showed that there were an interaction between nanoparticle phosphate rock and phosphate solubilizing fungi on the C-organic after one month incubation. Nanoparticle phosphate rock had affected on C-organic, P-potential and base saturation significantly, but had not affected on P-retention. Utilization of phosphate solubilizing fungi as soil ameliorant had affected significantly on P-potential and C-organic, but had not affected on P-retention and base saturation. Nanoparticle phosphate rock with a dose of 6% had the best effect on increase P-potential and base saturation

scholarly journals Biochar as soil amendment: Impact on chemical properties and corn nutrient uptake in a Podzol

2016 ◽  
Vol 96 (4) ◽  
pp. 400-412 ◽  
Author(s):  
A.B. Syuhada ◽  
J. Shamshuddin ◽  
C.I. Fauziah ◽  
A.B. Rosenani ◽  
A. Arifin
Keyword(s):  
Nutrient Uptake ◽  
Chemical Properties ◽  
Total N ◽  
Fertilized Soil ◽  
Randomized Design ◽  
Exchangeable Al ◽  
Completely Randomized Design ◽  
Healthy Growth ◽  
The Impact ◽  
Biochar Amendment

A study was conducted to investigate the impact of biochar amendment on chemical properties and corn nutrient uptake in a sandy Podzol soil. Four rates of biochar (0, 5, 10, and 15 g kg−1) and two rates of inorganic fertilizer (0 and local recommendation rate for corn) were randomly applied to a completely randomized design with four replicates. Corn was grown for 45 d in a glasshouse using sandy Podzol. The increase in pH of the soil was concomitant with a decrease of exchangeable Al. The fertilized soil significantly increased total N, with a concomitant decrease in soil pH due to a N nitrification. Positive changes did occur in the soil due to biochar application, leading to a significant increase in dry matter yield and corn height. Corn N and K uptakes were significantly increased by the addition of biochar, but the same was not true for Ca and Mg. However, it was found that the concentrations of N, Ca, and Mg in the corn tissue were still lower than their critical level. Our results demonstrate that application of biochar alone is not able to supply enough nutrients for the healthy growth of corn.

scholarly journals Penggunaan Pupuk Organik Cair Sebagai Media Produksi Inokulan Azotobacter Chroococcum

Agrologia ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Reginawanti Hindersah ◽  
H Yulina ◽  
Ane Nurbaity
Keyword(s):  
Organic Fertilizer ◽  
Scaling Up ◽  
Azotobacter Chroococcum ◽  
Growth Media ◽  
Total N ◽  
Support Cell ◽  
Commercial Scale ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Set Up

Azotobacter chroococcum based biofertilizers have been widely used in the production of environmentally agriculture. For commercial scale, cheap growth media for Azotobcter inoculant  will affect the quality and price of liquid biofertilizer. Growth medium should support cell proliferation as well as its  nitrogen fixation and phytohormone production.  The objective of this study was to determine whether  liquid organic fertilizer (LOF) containing low N can be used as a medium for scaling up  A. chroococcum inoculant without lowering the survival of bacteria and its activity in N2 fixation and phytohormones production. LOF generally contains a complete nutrient although in a small quantities. Experiment was set up in completely randomized design which tested two types of commercial LOF. The results showed that both of  LOF were only able to support cell growth up to 72 hours and at 96 hours cell viability began to decline compared to the control medium,  free-N Ashby. At 72 hour total N and cytokinin  concentration in LOF was lower than those in Ashby media. This study revealed  that LOF  could be used as A. chroococcum growth media, but without optimization of its composition, cell density and cytokinin content would be lower than those of inoculant  in Ashby Media.

scholarly journals Pengaruh pH terhadap Produksi Antibakteri oleh Bakteri Asam Laktat dari Usus Itik

2017 ◽  
Vol 15 (3) ◽  
Author(s):  
Rudy Sutrisna ◽  
Christina Nugroho Ekowati ◽  
Edelina Sinaga Sinaga
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Analysis Of Variance ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Effect Of Ph ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to determine the effect of pH on the production of antibacterial by lactic acid bacteria. This study uses a completely randomized design factorial 3X 5 (3 X 5 treatment of bacterial isolates pH) with three replications. The first factor consisted of pH 4, pH 5, pH 6, pH 7, pH 8, and the second factor that isolates B2, B7, B8, whereas the parameters consists of a clear zone. The amount of production is determined by the size of the diameter of antibacterial inhibition zone against Salmonella sp. Differences in the production of antibacterial determined based on the results of analysis of variance. The treatment significantly further test BNT at 5% level. The results showed that the effect of media pH test based on the potential for the production of antibacterial effect. The diameter of the largest clear zone produced by B7 isolates at pH 6 was 17.2 mm, 17.1 mm B8 and B2 at pH 7 at 16.7 mm. The smallest diameter of the clear zone produced by B8 at pH 4 was 15.1 mm, B2 at pH 6 there is a clear zone of 12.7 mm and isolate B7 at pH 5 of 11.5 mm Keywords: Antibacterial, Lactic Acid Bacteria, pH, Salmonella sp, Clear zone

scholarly journals ISOLASI DAN SELEKSI BAKTERI SEBAGAI AGEN BIOREMEDIASI LIMBAH CAIR PABRIK KAYU PUTIH

2018 ◽  
Vol 19 (2) ◽  
Author(s):  
Tri Rahayuningsih ◽  
Umi Isnatin ◽  
Parwi Parwi
Keyword(s):  
Organic Fertilizer ◽  
Phenol Degradation ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Solid Media ◽  
Randomized Design ◽  
Phenol Compound ◽  
Identification Of Bacteria ◽  
Completely Randomized Design ◽  
Biochemical Testing

This study aims to isolate and select bacteria as a bioremediation agent for wastewater of cajeput factory. The waterwaste of cajuput factory has not been utilized for liquid organic fertilizer because it still contains cajeput oil (phenol compound) which can disrupt plant growth. Therefore it is necessary to look for bacteria that have the ability to integrate of management waterwaste. Waterwaste samples is taken by random sampling in tri locations, namely the initial shelter, the second shelter and the waste disposal area. Bacteria are developed in liquid media, then isolated and identified on solid media. Identification of bacteria based on colony morphology, cell morphology and biochemical testing. Phenol degradation test was carried out using a completely randomized design with tri replications. Treatment of types of bacterial isolates namely B0 = Control, B1 = Bacillus, B2 = Psedomonas, B3 = Flavobacterium. The results showed that there were tri bacterial isolates found in wastewater of cajuput factory namely Bacillus sp, Pseudomonas sp and Flavobacterium sp. The highest amount of bacterial colonies was found in waste shelters of 2.41 x 106 colonies. The most effective bacteria in degrading phenol is Pseudomonas sp

scholarly journals Nutritional value and physical and chemical characteristics of white mulberry tree using different conservation methods for ruminant feed

2018 ◽  
Vol 39 (2) ◽  
pp. 771 ◽  
Author(s):  
Américo Fróes Garcez Neto ◽  
Janielen Da Silva ◽  
Eduardo Michelon do Nascimento ◽  
Jean Carlos Steinmacher Lourenço ◽  
Sergio Rodrigo Fernandes
Keyword(s):  
Ph Value ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Total N ◽  
Randomized Design ◽  
Aerobic Stability ◽  
Completely Randomized Design ◽  
White Mulberry ◽  
Physical And Chemical ◽  
Mulberry Tree

This study was carried out to evaluate the white mulberry tree (Morus alba) as a feedstuff for ruminants in natura, as hay and as silage. A completely randomized design with five treatments and three replicates was used, where the treatments were five types of mulberry forage: (1) in natura after cutting; (2) hay; (3) silage of mulberry in natura; (4) silage of wilted mulberry and (5) silage of mulberry with ground ear corn. The content of crude protein (CP) did not differ between the silages (average of 19.03% of dry matter - DM). The CP content of the silages was higher than in mulberry hay (15.26% DM) and similar to mulberry after cutting (17.00% DM). The neutral detergent fiber (NDF) content did not differ between the silages (34.84% DM on average), but the NDF of silage with ear corn was lower than the mulberry hay (33.77 vs. 39.36% DM). The acid detergent fiber (ADF) content did not differ between silages and mulberry hay (30.66% DM on average). The lowest value for ADF was observed in the mulberry after cutting (26.06% DM). The aerobic stability of the silages was not broken during the seven days of aerobic exposure. The pH of silage of mulberry in natura was similar to silage of wilted mulberry (4.80 on average), while the silage with ear corn showed a lower pH value (4.58) compared to the other silages. The buffering capacity (BC) did not differ between the silage with ear corn and silage of wilted mulberry (84.91 meq 100 g DM-1 on average), while a lower value was observed for silage of mulberry in natura (56.69 meq 100 g DM-1). The highest value for ammonia nitrogen (NH3-N) was demonstrated in the silage of mulberry in natura (13.08% total N), and the lowest values were observed in the silage with ear corn and silage of wilted mulberry (7.49 and 9.58% total N, respectively). Silage with 11% of ground ear corn is the best alternative use of white mulberry tree as forage for ruminant feeding, since this form presented low fiber content and a fermentative pattern suitable for the process of forage conservation.

scholarly journals Ammonia volatilization losses in Tanzania grass fertilized with urea

2015 ◽  
Vol 16 (1) ◽  
pp. 253-264 ◽  
Author(s):  
Cecílio Viega SOARES FILHO ◽  
Ulysses CECATO ◽  
Ossival Lolato RIBEIRO ◽  
Cláudio Fabrício da Cruz ROMA ◽  
Tatiane BELONI
Keyword(s):  
Ammonia Volatilization ◽  
Repeated Measurements ◽  
N Loss ◽  
N Losses ◽  
Total N ◽  
Factors Affecting ◽  
Randomized Design ◽  
Different Seasons ◽  
Completely Randomized Design ◽  
N Volatilization

<p>Gaseous losses are the main factors affecting the efficiency of nitrogenous fertilizers in pastures. To evaluate NH<sub>3</sub>-N volatilization losses in Tanzania grass fertilized with urea in autumn, spring and summer, a completely randomized design with repeated measurements over time and fifteen replicates was used. Plots were represented by urea levels (50; 100 and 150 kg ha<sup>-1</sup> N) and subplots by time after fertilization (1; 2; 3; 6; 9; 12 and 15 days). The interaction between fertilization leveland time after urea application was significant for the accumulated NH<sub>3</sub>-N volatilization. Urea application leads to higher percentage N losses in the first three days after application. The average cumulative NH<sub>3</sub>-N loss for the three occasions (different seasons of the year) was 28%, 20% and 16% of N applied for fertilizer doses of 50; 100 and 150 kg ha<sup>-1</sup>of N, respectively. The season of the year influenced NH<sub>3</sub>-N loss pattern and volume, with the lowest values recorded in spring, followed by summer and autumn. The cumulative NH<sub>3</sub>-N volatilization loss varies from 78 to 90% up to the third day after application of the total N-NH3 loss.</p>

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