scholarly journals Influence of diesel fuel on the composition and content of free amino acids in the green alga Acrosiphonia arcta

2021 ◽  
Vol 937 (2) ◽  
pp. 022010
Author(s):  
M Klindukh ◽  
E Dobychina ◽  
M Makarov ◽  
I Ryzhik

Abstract Arctic ecosystems are the most sensitive and vulnerable to anthropogenic impact, namely, the influence of petroleum products. The aim is to identify the effect of diesel fuel (DF) on the composition of free amino acids (FAA) in Acrosiphonia arcta in Barents Sea. The impact of DF concentrations of 20 - 3000 maximal permitted concentration (MPC) was analyzed. It was found that A. arcta contains 20 amino acids, 16 of which are proteinogenic and 8 are essential. The composition of the FAA pool did not change under the influence of DF, but the content of individual FAA and their total amount differed. The ratio of the dominant amino acids changes: at concentrations of DF up to 1000 MPC content of proline increased, and decreased at higher concentrations of DF. Under the influence of DF in concentration of 3000 MPC, taurine content significant increased. The least negative effect was exerted under the influence of DF concentration of 20 MPC, more significant - at the concentration more than 1000 MPC. Changes in the content of FAA are probably associated with the redirection of the synthesis pathways of amino acid groups, as well as the inhibitory effect of the toxicant on protein synthesis.

2020 ◽  
Vol 11 (5-2020) ◽  
pp. 92-102
Author(s):  
M.P. Klindukh ◽  
◽  
I.V. Ryzhik ◽  
E.O. Dobychina ◽  
◽  
...  

Data on the content of free amino acids (FAA) in four species of red algae are presented. The content of 20 amino acids was determined in the composition of FAA of the studied species. The largest amounts contained glutamic and aspartic acids, alanine, proline and taurine. The studied algae do not differ in the qualitative composition of protein amino acids, but differ in the presence and content of nonprotein ones. The content of most of the FAA and their summ was the highest for Phycodrysrubens, and in Palmaria palmatathe smallest.


2016 ◽  
Vol 36 (4) ◽  
pp. 558-565 ◽  
Author(s):  
Jinzhong Lin ◽  
Baozhen Hua ◽  
Zhiping Xu ◽  
Sha Li ◽  
Chengjie Ma

2021 ◽  
Author(s):  
Jing Yang ◽  
Yi Lin ◽  
Christopher Rensing ◽  
Liming Zhang ◽  
Biqing Zhou ◽  
...  

Abstract. Free amino acids (FAAs) in soil play an important role in the soil nitrogen cycle and plant nutrition. However, the attributing factors and migration characteristics of free amino acid pools in paddy soils after green manure application during the entire growth period of rice have not been elucidated. In this study, a single application of chemical fertilizer (CK) was used as a control under equal nitrogen, phosphorus and potassium conditions, and different application rates of milk vetch (15 000 kghm−2(CL), 30 000 kghm−2(CM) and 45 000 kg hm−2(CH)) were selected to investigate the dynamic of FAAs concentration and composition in paddy soil. Soil FAAs concentration at different growth stages under the same fertilization treatments was highest at the seedling stage and lowest at the tillering stage. The concentration of threonine, alanine, valine, isoleucine, leucine, phenylalanine was most abundant under different fertilization treatments during the growth period, accounting for 59.42 %–76.46 % of the respective FAAs pool. The application of milk vetch was shown to increase the soil FAAs concentration, especially glutamic acid, which increased by 368.17 %–680.78 %, but excessive application had an inhibitory effect. Soil pH, organic matter, protease, bacterial biomass and community were critical factors affecting the concentration of soil FAAs. Bacteroidetes, Firmicutes and Nitrospirae significantly affected the dynamics of FAAs in bacterial communities, and their total contribution rate was 56.89 %. FAAs displayed significant vertical profile characteristics, and the mobility of serine, glycine and proline was high. Conclusively, the application of milk vetch was able to significantly change the concentration and composition of soil FAAs, which may affect the capture of N by plants.


Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1170
Author(s):  
Eva Salazar ◽  
José Mª Cayuela ◽  
Adela Abellán ◽  
Estefanía Bueno-Gavilá ◽  
Luis Tejada

The aim of this work is to analyse the intramuscular fatty acids and the free amino (FAA) acids in Chato murciano dry-cured ham. There are several Mediterranean native pig breeds whose characteristics of derived products have been described, but the impact of lipolysis and proteolysis on Chato murciano dry-cured ham has not yet been studied. Fatty acids and free amino acids were determined in the fresh piece and at 14, 18, 22 and 24 months of manufacturing. Monounsaturated fatty acids are the majority in the neutral lipids and free fatty acid fractions. Lipolysis took place mainly until the 18th month, resulting in a decrease in the levels of fatty acids of neutral lipids (from 95.43% to 83.38%) and polar lipids (from 2.57% to 0.41%), accompanied by a corresponding increase in free fatty acids (from 2% to 16.21%). Neutral lipids hydrolysis provides the main free fatty acids as in other native breeds. Results for FAA showed an increase in concentration during the time preceding the 14th month. From this point onwards, until month 18, total FAA concentration remained stable, and the content decreased at the end of the processing (between months 22 and 24).


Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 518 ◽  
Author(s):  
Zahoor Ahmed ◽  
Muhammad Faisal Manzoor ◽  
Nabila Begum ◽  
Abbas Khan ◽  
Imranullah Shah ◽  
...  

The impact of thermo–ultrasound (TU) on the quality of fresh wheat plantlets juice is described in this study. Fresh wheat plantlets juice was treated with TU using ultrasound (US) bath cleaner with different treatment variables, including power (70%, 420 W), frequency (40 kHz), processing time (20 and 40 min) and temperature (30, 45 and 60 °C) for the determination of free amino acids, minerals, microbial loads and bioactive compounds. The treatments have non-significant effects in ºBrix, pH, and titratable acidity while a significant increase in non-enzymatic browning, viscosity, and cloud value. The TU treatment at 30 °C for 20 and 40 min has achieved the highest value of total phenolics, flavonoids, total antioxidant capacity, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), carotenoids, anthocyanin contents, chlorophyll (a + b), minerals and free amino acids than other treatments as well as untreated sample. A lightly visible variation in the color was observed among all treatments. TU treatments also showed a significant impact on the reduction of microbial loads at 60 °C for 40 min. The verdicts revealed that TU at low temperature a viable option to improve the quality of wheat plantlets juice at an industrial scale as compared to alone.


2020 ◽  
Author(s):  
Inna Ryzhik ◽  
Ekaterina Dobychina ◽  
Maria Klindukh ◽  
Dmitry Salahov ◽  
Yana Glukhikh

Macrophyte algae are perspective indicator species for assessing the environmental impact of pollutants. One of the ways to estimate the negative impact is to change the activity of enzymes of the antioxidant system. The possibility of using hydrogen peroxide, catalase and superoxide dismutase (SOD) is analyzed in the research for assessing the anthropogenic load on algae macrophytes of different systematic groups: Palmaria palmata (L.) O. Kuntze; Fucus vesiculosus L., Ulvaria obscura (Kützing) Gayral ex Bliding. The natural level of enzymes (in July) and its change under the influence of oil products (diesel fuel) on algae growing in the Barents sea were measured. It is shown that under natural conditions the content of hydrogen peroxide and catalase activity decrease in the series U. obscura < P. palmata < F. vesiculosus; the activity of SOD decreases in the series P. palmata < U. obscura < F. vesiculosus. When diesel fuel is introduced into the habitat of algae, the concentration of hydrogen peroxide in the cells of U. obscura and P. palmata decreases and in F. vesiculosus-increases. Catalase activity in P. palmata and U. obscura increases and catalase activity is not various in F. vesiculosus. The activity of SOD in the prototypes of G. obscura and P. palmata decreases and it remains unchanged in F. vesiculosus. It is shown that the enzyme complex Ulvaria obscura can be used to assess the impact of diesel fuel and only SOD can be used in P. palmata. No reaction of SOD and CAT to the presence of petroleum products was detected in F. vesiculosus.


Author(s):  
Luan Dos Santos Silva ◽  
Paula Tereza de Souza e Silva ◽  
Ítalo Herbert Lucena Cavalcante

This study evaluated the impact of fulvic acid and free amino acids on paclobutrazol soil residue, their absorption and effects on ‘Keitt’ mango grown in tropical semi-arid environmental conditions. The experiment was carried out from 2017 to 2018 simultaneously in two orchards with the same plants and management characteristics, located in Cabrobó, Pernambuco, Brazil. The experimental design was randomized blocks with four treatments, five replications and four plants per replication. The treatments consisted of paclobutrazol combinations with acid fulvic and free amino acids, as follows: Treatment 1: paclobutrazol + water (control); Treatment 2: paclobutrazol + fulvic acids; Treatment 3: paclobutrazol + free amino acids; and Treatment 4: paclobutrazol + fulvic acids + free amino acids. According to the results, the use of fulvic acids, free amino acids or both affects the paclobutrazol absorption by 'Keitt' mango. The addition of fulvic acid to the paclobutrazol improves the absorption of this molecule by the plant, with greater inhibition of vegetative growth of 'Keitt' mango and lower soil residues.


1991 ◽  
Vol 83 (1) ◽  
pp. 136-143 ◽  
Author(s):  
L. Bray ◽  
D. Chriqui ◽  
K. Gloux ◽  
D. Le Rudulier ◽  
M. Meyer ◽  
...  

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