scholarly journals The effect of water content and carbon source addition on lipase production from Aspergillus aculeatus Ms.11 using spent coffee ground

2021 ◽  
Vol 948 (1) ◽  
pp. 012055
Author(s):  
A Yuliyanti ◽  
M C D Manullang ◽  
M Ilmi

Abstract Lipase is one of the industrially important enzymes, however, the production needs costly substrate. To overcome the problem, we developed an effective medium formulation to produce lipase from indigenous lipolytic mould Aspergillus aculeatus Ms.11 using spent coffee ground. We observed the effect of additional glucose and olive oil, and the influence of water content on lipase production. The experiments were done using the Solid-State Fermentation (SSF) method for 7 days. The results show that optimum lipase production on substrates with additional glucose and olive oil observed on day 4 with lipase activity of 16.296 U/mL and lipase productivity of 150.32 U/g/day. The optimum water content from the results is 50%. The highest lipase activity obtained using the water content is 291.80 U/mL, while the highest lipase productivity is 106.32 U/g/day. The results showed that water content as well as the addition of glucose and olive oil, affects lipase productivity of Aspergillus aculeatus Ms.11 on spent coffee ground. Further studies to optimise the production condition are suggested.

2011 ◽  
Vol 261-263 ◽  
pp. 1760-1766
Author(s):  
Hong Jun Liu ◽  
Ying Guo ◽  
Wei Shan ◽  
Pi Xiang Wang

The method of evaluating the effect of geogrid reinforcement mainly is the finite element method at present. The interface between geogrid and soil is set to contacting element normally. The tangential stiffness expression has five parameters totally. The interaction characteristics of geosynthetics and filled soil are related to the soil type and water content. We designed the experimental research program, using direct shear friction test, study the five parameters of the interface between geogrid and soil varied with water content and soil type. The experimental results show that: Whether weathered sands or silty clay, the value of the parameters (k1,n,c,φ) increase with the increase of water content, when reaching the peak point, then the value of the parameters (k1,n,c,φ) will decreases with the increase of water content. The water content corresponding to the peak is near the optimum water content. The value of the parameters (Rf) decreases with the increase of water content, when reaching a minimum, the value of the parameters (Rf) will increase with the increase of water content. The water content corresponding to the minimum also is near the optimum water content. The research results can provide reliable basis for the selection of evaluation parameters.


2012 ◽  
Vol 256-259 ◽  
pp. 481-487
Author(s):  
Xiao Ming Yi ◽  
Song Gen Wang ◽  
Zhen Qing Liu ◽  
Gang Xu

Hydrophilic characteristic of silt soil in Yellow River alluvial plain was studied in order to reveal the water content changes of silt. Then strength tests were used to research how engineering parameters such as elastic modulus, cohesion and friction angle change at different water content. The results show that high permeability coefficient and strong capillary are main factors to increase the water content, and the influence of capillary rising is greater than that of rainfall infiltration. The strength characteristic of silt soil is similar to the character of non-cohesive soil in low water content and that of clay in high water content. If the water content is greater than optimum water content, the elastic modulus and cohesion of silt shall decay obviously. Friction angle decreases dramatically as well, when the soil is saturated.


2003 ◽  
Vol 2 (3) ◽  
pp. 368
Author(s):  
Hongkyu Yoon ◽  
Albert J. Valocchi ◽  
Charles J. Werth

2021 ◽  
Vol 765 (1) ◽  
pp. 012089
Author(s):  
R Taufik ◽  
M Mohamad ◽  
R Wannahari ◽  
N F Shoparwe ◽  
WHW Osman ◽  
...  

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 90
Author(s):  
Andrzej Bryś ◽  
Joanna Bryś ◽  
Marko Obranović ◽  
Dubravka Škevin ◽  
Szymon Głowacki ◽  
...  

The olive oil industry represents an important productive sector in the Mediterranean basin countries. Olive stone is an essential by-product generated in the olive oil extraction industries and it represents roughly 10% by weight of the olive fruit. The seeds of pickled olives are also a significant waste product. In the present study, we have investigated the possibility of the use of differential scanning calorimetry for the thermal characterization of seeds from green and black pickled olives from Croatia. The differential scanning calorimeter (DSC) with a normal pressure cell equipped with a cooling system was used to determine the thermal properties of seeds from olives. The following analyses were also performed: the determination of calorific values in a pressure bomb calorimeter, the determination of initial water content, the determination of changes of water content during drying at the temperatures of 30 °C, 50 °C and 80 °C, the determination of a percentage content of seeds mass to the mass of the whole olives, and the determination of ash content. Seeds from olives are characterized by very good parameters as a biomass. The analyzed olive seeds were characterized by low water content, low ash content, and a relatively high caloric value.


2021 ◽  
pp. 002199832110022
Author(s):  
Ahmed Alhelal ◽  
Zaheeruddin Mohammed ◽  
Shaik Jeelani ◽  
Vijaya K Rangari

Semi-crystalline carbon biochar is derived from spent coffee grounds (SCG) by a controlled pyrolysis process at high temperature/pressure conditions. Obtained biochar is characterized using XRD, SEM, and TEM techniques. Biochar particles are in the micrometer range with nanostructured morphologies. The SCG biochar thus produced is used as reinforcement in epoxy resin to 3 D print samples using the direct-write (DW) method with 1 and 3 wt. % loadings. Rheology results show that the addition of biochar makes resin viscous, enabling it to be stable soon after print; however, it could also lead to clogging of resin in printer head. The printed samples are characterized for chemical, thermal and mechanical properties using FTIR, TGA, DMA and flexure tests. Storage modulus improved with 1 wt. % biochar addition up to 27.5% and flexural modulus and strength increased up to 55.55% and 43.30% respectively. However, with higher loading of 3 wt. % both viscoelastic and flexural properties of 3D printed samples drastically reduced thus undermining the feasibility of 3D printing biochar reinforced epoxies at higher loadings.


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