The Impact of Ammonium Chloride-Based Erythrocyte Lysis Process on Banked Adipose-Derived Stem Cells

2021 ◽  
Author(s):  
Zifei Li ◽  
Dali Mu ◽  
Chunjun Liu ◽  
Minqiang Xin ◽  
Su Fu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ammonium Chloride ◽  
Adipose Derived Stem Cells ◽  
Erythrocyte Lysis ◽  
The Impact

scholarly journals In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1574 ◽  
Author(s):  
Michalina Ehlert ◽  
Aleksandra Radtke ◽  
Tomasz Jędrzejewski ◽  
Katarzyna Roszek ◽  
Michał Bartmański ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Line ◽  
Osteogenic Potential ◽  
Biological Research ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
Nanoporous Surface ◽  
Titania Coatings ◽  
The Impact

In vitro biological research on a group of amorphous titania coatings of different nanoarchitectures (nanoporous, nanotubular, and nanosponge-like) produced on the surface of Ti6Al4V alloy samples have been carried out, aimed at assessing their ability to interact with adipose-derived mesenchymal stem cells (ADSCs) and affect their activity. The attention has been drawn to the influence of surface coating architecture and its physicochemical properties on the ADSCs proliferation. Moreover, in vitro co-cultures: (1) fibroblasts cell line L929/ADSCs and (2) osteoblasts cell line MG-63/ADSCs on nanoporous, nanotubular and nanosponge-like TiO2 coatings have been studied. This allowed for evaluating the impact of the surface properties, especially roughness and wettability, on the creation of the beneficial microenvironment for co-cultures and/or enhancing differentiation potential of stem cells. Obtained results showed that the nanoporous surface is favorable for ADSCs, has great biointegrative properties, and supports the growth of co-cultures with MG-63 osteoblasts and L929 fibroblasts. Additionally, the number of osteoblasts seeded and cultured with ADSCs on TNT5 surface raised after 72-h culture almost twice when compared with the unmodified scaffold and by 30% when compared with MG-63 cells growing alone. The alkaline phosphatase activity of MG-63 osteoblasts co-cultured with ADSCs increased, that indirectly confirmed our assumptions that TNT-modified scaffolds create the osteogenic niche and enhance osteogenic potential of ADSCs.

scholarly journals The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer

2020 ◽  
Vol 21 (8) ◽  
pp. 2869
Author(s):  
Felix Grambow ◽  
Rico Rutkowski ◽  
Fred Podmelle ◽  
Katrin Schmoeckel ◽  
Florian Siegerist ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Small Sample Size ◽  
Subcutaneous Fat ◽  
Stromal Vascular Fraction ◽  
Small Sample ◽  
Relative Distribution ◽  
Adipose Derived Stem Cells ◽  
Female Patients ◽  
The Impact

The local anesthetic lidocaine, which has been used extensively during liposuction, has been reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer. We evaluated the effect of lidocaine on the distribution, number, and viability of adipose-derived stem cells (ASCs), preadipocytes, mature adipocytes, and leukocytes in the fatty and fluid portion of the lipoaspirate using antibody staining and flow cytometry analyses. Adipose tissue was harvested from 11 female patients who underwent liposuction. Abdominal subcutaneous fat tissue was infiltrated with tumescent local anesthesia, containing lidocaine on the left and lacking lidocaine on the right side of the abdomen, and harvested subsequently. Lidocaine had no influence on the relative distribution, cell number, or viability of ASCs, preadipocytes, mature adipocytes, or leukocytes in the stromal-vascular fraction. Assessing the fatty and fluid portions of the lipoaspirate, the fatty portions contained significantly more ASCs (p < 0.05), stem cells expressing the preadipocyte marker Pref-1 (p < 0.01 w/lidocaine, p < 0.05 w/o lidocaine), and mature adipocytes (p < 0.05 w/lidocaine, p < 0.01 w/o lidocaine) than the fluid portions. Only the fatty portion should be used for transplantation. This study found no evidence that would contraindicate the use of lidocaine in lipotransfer. Limitations of the study include the small sample size and the inclusion of only female patients.

The impact of short-term refrigeration of human lipoaspirate on adipose-derived stem cells and adipocytes

2015 ◽  
Vol 68 (1) ◽  
pp. 137-139 ◽  
Author(s):  
Wei Z. Wang ◽  
Xin-Hua Fang ◽  
Shelley J. Williams ◽  
Linda L. Stephenson ◽  
Richard C. Baynosa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Short Term ◽  
The Impact

scholarly journals The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging

2020 ◽  
Vol 2020 ◽  
pp. 1-19
Author(s):  
Feng Qin ◽  
Jiuzuo Huang ◽  
Wenchao Zhang ◽  
Mingzi Zhang ◽  
Zhenjiang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Adipose Derived Stem Cells ◽  
Paracrine Effects ◽  
Skin Photoaging ◽  
Uvb Exposure ◽  
Underlying Mechanisms ◽  
The Impact

Background. Human dermal fibroblasts (HDFs) are the primary cells in skin and are associated with UVB-induced skin photoaging. Adipose-derived stem cells (ASCs) have been proposed as a treatment for skin aging. The goal of this study was to investigate paracrine mechanisms by which ASCs repair HDFs damage from UVB exposure. Methods. ASCs were cocultured with UVB-irradiated and nonirradiated HDFs. We compared HDF senescence, proliferation, migration, oxidative stress, and cytokine expression. In a nude mouse UVB-induced photoaging model, ASCs were injected subcutaneously, and skin samples were collected weekly between postoperative weeks 3 through 7. Histological analysis, PCR, ELISA, and immunohistochemistry were used to analyze the effect of ASCs. Results. Compared with UVB-irradiated HDFs, nonirradiated HDFs showed higher proliferation and migration, reduced apoptosis, and fewer senescent cells when cocultured with ASCs. The expression of extracellular matrix-related cytokines was also regulated by ASCs. In addition, ASCs effectively reversed UVB-induced skin photoaging in vivo. We propose that ASCs more robustly coordinate healthy HDFs than UVB-damaged HDFs to repair aging skin. Conclusions. ASCs improved the function of both UVB-damaged and healthy HDFs through paracrine effects. However, the impact of ASCs on healthy HDFs was greater than UVB-damaged HDFs. These findings help to elucidate the underlying mechanisms of the skin rejuvenation effect of ASCs.

scholarly journals Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Nina Krešić ◽  
Ivana Šimić ◽  
Ivana Lojkić ◽  
Tomislav Bedeković
Keyword(s):  
Stem Cells ◽  
Therapeutic Potential ◽  
Ex Vivo ◽  
Adipose Derived Stem Cells ◽  
Morphological Alterations ◽  
Undifferentiated State ◽  
Passage Number ◽  
The Impact ◽  
First Time ◽  
Morphology Characteristics

Although canine adipose derived stem cells (cASCs) morphology characteristics and differentiation ability are well documented, transcriptome alterations of undifferentiated cASCs during ex vivo cultivation remain unknown. Here we demonstrate, for the first time, the transcriptome composition of isolated cASCs in undifferentiated state originating from six donors. Transcriptome changes were monitored during ex vivo cultivation between passage 3 (P3) and P5, which are mostly used in therapy. Influence of donors’ age in given passage number on transcriptome composition was also investigated. Cultivation from P3 to P5 resulted in 16 differentially expressed genes with cooverexpression of pluripotency and self-renewal transcription factors genes SOX2 and POU5F1 dominant in old donors’ cells. Furthermore, cASCs demonstrated upregulation of IL-6 in young and old donors’ cells. In addition, ex vivo cultivation of cASCs revealed well-known morphological alterations accompanied with decrease in expression of CD90 and CD44 markers in P4 and higher monitored by flow cytometry and successful osteo- and chondrodifferentiation but inefficient adipodifferentiation in P3. Our results revealed the impact of ex vivo cultivation on nature of cells. Correlation of transcriptome changes with secretome composition is needed and its further impact on therapeutic potential of cASCs remains to be evaluated in clinical trials.

scholarly journals Effect of Pig-Adipose-Derived Stem Cells’ Conditioned Media on Skin Wound-Healing Characteristics In Vitro

2021 ◽  
Vol 22 (11) ◽  
pp. 5469
Author(s):  
Joanna Wiśniewska ◽  
Magda Słyszewska ◽  
Karolina Stałanowska ◽  
Katarzyna Walendzik ◽  
Marta Kopcewicz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Conditioned Media ◽  
Adipose Derived Stem Cells ◽  
Skin Wound Healing ◽  
Cell Functions ◽  
The Impact

The primary mechanism by which adipose-derived stem cells (ASCs) exert their reparative or regenerative potential relies predominantly on paracrine action. Secretory abilities of ASCs have been found to be amplified by hypoxia pre-conditioning. This study investigates the impact of hypoxia (1% O2) on the secretome composition of pig ASCs (pASCs) and explores the effect of pASCs’ conditioned media (CM) on skin cell functions in vitro and the expression of markers attributed to wound healing. Exposure of pASCs to hypoxia increased levels of vascular endothelial growth factor (VEGF) in CM-Hyp compared to CM collected from the cells cultured in normoxia (CM-Nor). CM-Hyp promoted the migratory ability of pig keratinocytes (pKERs) and delayed migration of pig dermal fibroblasts (pDFs). Exposure of pKERs to either CM-Nor or CM-Hyp decreased the levels of pro-fibrotic indicators WNT10A and WNT11. Furthermore, CM-Hyp enhanced the expression of KRT14, the marker of the basal epidermis layer. In contrast, CM-Nor showed a stronger effect on pDFs manifested by increases in TGFB1, COL1A1, COL3A1, and FN1 mRNA expression. The formation of three-dimensional endothelial cell networks was improved in the presence of CM-Hyp. Overall, our results demonstrate that the paracrine activity of pASCs affects skin cells, and this property might be used to modulate wound healing.

The Impact of Long-Term In Vitro Expansion on the Senescence-Associated Markers of Human Adipose-Derived Stem Cells

2012 ◽  
Vol 166 (8) ◽  
pp. 2101-2113 ◽  
Author(s):  
Wan Kamarul Zaman Wan Safwani ◽  
Suzana Makpol ◽  
Somasundaram Sathapan ◽  
Kien Hui Chua
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
In Vitro Expansion ◽  
The Impact

scholarly journals In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Alona Zlatska ◽  
Inna Gordiienko ◽  
Roman Vasyliev ◽  
Dmitriy Zubov ◽  
Olga Gubar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Medium ◽  
Metabolic Activity ◽  
Proliferation Rate ◽  
Population Doubling ◽  
Growth Media ◽  
Deuterium Content ◽  
Adipose Derived Stem Cells ◽  
The Impact

In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.

scholarly journals SDF-1α Gene-Activated Collagen Scaffold Restores Pro-Angiogenic Wound Healing Features in Human Diabetic Adipose-Derived Stem Cells

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 160
Author(s):  
Ashang L. Laiva ◽  
Fergal J. O’Brien ◽  
Michael B. Keogh
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Collagen Scaffold ◽  
Diabetic Patients ◽  
Matrix Remodeling ◽  
Adipose Derived Stem Cells ◽  
Cell Therapies ◽  
Gene And Protein Expression ◽  
The Impact

Non-healing diabetic foot ulcers (DFUs) can lead to leg amputation in diabetic patients. Autologous stem cell therapy holds some potential to solve this problem; however, diabetic stem cells are relatively dysfunctional and restrictive in their wound healing abilities. This study sought to explore if a novel collagen–chondroitin sulfate (coll–CS) scaffold, functionalized with polyplex nanoparticles carrying the gene encoding for stromal-derived factor-1 alpha (SDF-1α gene-activated scaffold), can enhance the regenerative functionality of human diabetic adipose-derived stem cells (ADSCs). We assessed the impact of the gene-activated scaffold on diabetic ADSCs by comparing their response against healthy ADSCs cultured on a gene-free scaffold over two weeks. Overall, we found that the gene-activated scaffold could restore the pro-angiogenic regenerative response in the human diabetic ADSCs similar to the healthy ADSCs on the gene-free scaffold. Gene and protein expression analysis revealed that the gene-activated scaffold induced the overexpression of SDF-1α in diabetic ADSCs and engaged the receptor CXCR7, causing downstream β-arrestin signaling, as effectively as the transfected healthy ADSCs. The transfected diabetic ADSCs also exhibited pro-wound healing features characterized by active matrix remodeling of the provisional fibronectin matrix and basement membrane protein collagen IV. The gene-activated scaffold also induced a controlled pro-healing response in the healthy ADSCs by disabling early developmental factors signaling while promoting the expression of tissue remodeling components. Conclusively, we show that the SDF-1α gene-activated scaffold can overcome the deficiencies associated with diabetic ADSCs, paving the way for autologous stem cell therapies combined with novel biomaterials to treat DFUs.

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