Correction to: Prevalence and Antimicrobial Susceptibility of Salmonella Serovars Isolated from U.S. Retail Ground Pork by P.R. Broadway et al. Foodborne Pathogens and Disease 2021;18(3)219–227. DOI: 10.1089/fpd.2020.2853

A mixed culture of different isolates of Salmonella serovar I 4,[5], 12:i:- was compared to a mixed culture of reference Salmonella serovars as well as non-pathogenic Escherichia coli surrogates.. The two groups of Salmonella were compared for their resistance to commonly used pork carcass interventions, survival in ground pork and thermal resistance in ground pork. There were no observed differences between the response of the two different groups of Salmonella serovars and the non-pathogenic E. coli surrogates within intervention type. There were no observed differences in the recovery and survival of the two different groups of Salmonella serovars in pork which had been treated with interventions, ground and stored at 5 o C for two weeks. Finally, there were no observed differences in heat resistance between the two different groups of Salmonella serovars in ground pork which had been treated with interventions, ground and stored at 5 o C for two weeks. However, there were observed differences in heat resistance in both groups of Salmonella serovars associated with refrigerated storage. The heat resistance of both groups of Salmonella serovars decreased after refrigerated storage. The results of these experiments demonstrate that there were no observed differences between the responses of Salmonella serovar I 4,[5], 12:i:- when compared to the reference Salmonella serovars to commonly used interventions in the pork industry, and therefore do not present a unique challenge to the pork industry.

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Salmonella Serovars Isolated from Brown Rats (Rattus norvegicus) from Grenada, West Indies: Prevalence and Antimicrobial Susceptibility

Microbiology Research Journal International ◽

10.9734/mrji/2018/44563 ◽

2018 ◽

Vol 25 (1) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Ravindra Sharma ◽

Keshaw Tiwari ◽

Gitanjali Arya ◽

Victor Amadi ◽

Darnell Blackman ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

Rattus Norvegicus ◽

West Indies ◽

Salmonella Serovars

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Prevalence and Antimicrobial Susceptibility of Campylobacter spp. in Oklahoma Conventional and Organic Retail Poultry

The Open Microbiology Journal ◽

10.2174/1874285801408010130 ◽

2014 ◽

Vol 8 (1) ◽

pp. 130-137 ◽

Cited By ~ 24

Author(s):

Aneesa Noormohamed ◽

Mohamed K Fakhr

Keyword(s):

Multidrug Resistance ◽

Antimicrobial Resistance ◽

Foodborne Pathogens ◽

Antimicrobial Susceptibility ◽

Grocery Stores ◽

Campylobacter Spp

Campylobacteris one of the most important foodborne pathogens that cause bacterial gastroenteritis.This study was conducted to investigate the prevalence and antimicrobial resistance ofCampylobacterin conventional and organic retail poultry samples purchased from grocery stores in Tulsa, Oklahoma.One hundred and fifty six chilled retail chicken samples (85 conventional and 71 organic) and 65 chilled retail conventional turkey samples were collected in this study. The prevalence ofCampylobacterin the conventional chicken samples 32/85 (38%) was higher than in the organic ones 21/71 (30%). The prevalence ofCampylobacterin the conventional turkey samples was 11/65 (17%). Of the 53 positive chicken samples, 42 wereC. jejuni, 8 wereC. coliand three isolates were contaminated with both species. Of the 11 positive turkey samples, 8 containedC. jejuniand 3 harboredC. coliisolates. The antimicrobial susceptibility of one hundred and forty nine recoveredCampylobacterisolates (130 chickens and 19 turkeys) towards sixteen antimicrobials was determined. The majority of the recovered turkey isolates (13/19) showed resistance to more than 7 antimicrobials while most of the recovered chicken ones (82/130) were resistant to 5 to 7 antimicrobials. Multidrug resistance was not limited to isolates from conventional sources but was also available in isolates of an organic background and was generally lower inC. jejuniisolates when compared to theC. coliones.

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Antimicrobial susceptibility of Salmonella serovars isolated from edible offal and carcasses of slaughtered poultry in the state of Tocantins, Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n4p2661 ◽

2015 ◽

Vol 36 (4) ◽

pp. 2661 ◽

Cited By ~ 2

Author(s):

Silvia Minharro ◽

Cristiane Alves Nascimento ◽

Jean Paulo Galletti ◽

Tainá Japiassú Merisse ◽

Adriana Carla Floresta Feitosa ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

The State ◽

Salmonella Serovars

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Antimicrobial Susceptibility of Foodborne Pathogens in Organic or Natural Production Systems: An Overview

Foodborne Pathogens and Disease ◽

10.1089/fpd.2008.0095 ◽

2008 ◽

Vol 5 (6) ◽

pp. 721-730 ◽

Cited By ~ 20

Author(s):

Megan E. Jacob ◽

James Trent Fox ◽

Shelby L. Reinstein ◽

T.G. Nagaraja

Keyword(s):

Foodborne Pathogens ◽

Antimicrobial Susceptibility ◽

Production Systems ◽

Natural Production

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Prevalence, antimicrobial susceptibility pattern, and associated factors of Salmonella and Shigella among food handlers in Adigrat University student’s cafeteria, Northern Ethiopia, 2018

10.21203/rs.2.22938/v4 ◽

2020 ◽

Author(s):

Haftom Legese ◽

Tsega Kahsay ◽

Aderajew Gebrewahd ◽

Brhane Berhe ◽

Berhane Fseha ◽

...

Keyword(s):

Foodborne Pathogens ◽

Antimicrobial Susceptibility ◽

Associated Factors ◽

Public Health Problem ◽

Hand Washing ◽

Control Measures ◽

Major Public Health Problem ◽

Northern Ethiopia ◽

Food Handlers ◽

Cross Sectional

Abstract Background: Food handlers play a significant role in the transmission of foodborne infections. Salmonella and Shigella are the most common foodborne pathogens and their infections are a major public health problem globally. Thus, this study aimed to determine the prevalence, antimicrobial susceptibility patterns, and associated factors of Salmonella and Shigella among food handlers.Methodology: A cross-sectional study was conducted from March to August 2018 at Adigrat University student cafeteria, Northern Ethiopia. Data on socio-demographic and associated factors were collected using a structured questionnaire. Fresh stool samples were collected from 301 food handlers and transported to Adigrat University Microbiology Laboratory. Bacterial isolation and antimicrobial susceptibility test were performed using standard bacteriological methods. Data analysis was performed using SPSS version 22 and P < 0.05 with a corresponding 95% confidence interval was considered statistically significant.Results: A total of 301 food handlers were included in this study. The majority of study participants were females 265 (88.0 %). About 22 (7.3%) and 11 (3.7%) of food handlers were found to be positive for Salmonella and Shigella respectively. Hand washing after using a bathroom with water only, hand washing after using the bathroom, hand washing after touching dirty materials, hand washing before food handling, and fingernails status were significant associated factors identified. None of the Salmonella and Shigella isolates were sensitive to ampicillin. On the other hand, low resistance was found for chloramphenicol, ceftriaxone, and ciprofloxacin.Conclusion: The present study revealed that the prevalence of Salmonella and Shigella among food handlers was 22 (7.3%) and 11 (3.7%) respectively. Such colonized food handlers can contaminate food, drinks and could serve as a source of infection to consumers via the food chain. This indicates that there is a need for strengthened infection control measures to prevent Salmonella and Shigella transmission in the students’ cafeteria.

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Prevalence, antimicrobial susceptibility pattern, and associated factors of Salmonella and Shigella among food handlers in Adigrat University student’s cafeteria, Northern Ethiopia, 2018

10.21203/rs.2.22938/v6 ◽

2020 ◽

Author(s):

Haftom Legese ◽

Tsega Kahsay ◽

Aderajew Gebrewahd ◽

Brhane Berhe ◽

Berhane Fseha ◽

...

Keyword(s):

Foodborne Pathogens ◽

Antimicrobial Susceptibility ◽

Associated Factors ◽

Public Health Problem ◽

Hand Washing ◽

Control Measures ◽

Major Public Health Problem ◽

Northern Ethiopia ◽

Food Handlers ◽

Cross Sectional

Abstract Background: Food handlers play a significant role in the transmission of foodborne infections. Salmonella and Shigella are the most common foodborne pathogens and their infections are a major public health problem globally. Thus, this study aimed to determine the prevalence, antimicrobial susceptibility patterns, and associated factors of Salmonella and Shigella colonization among food handlers.Methodology: A cross-sectional study was conducted from March to August 2018 at Adigrat University student cafeteria, Northern Ethiopia. Data on socio-demographic and associated factors were collected using a structured questionnaire. Fresh stool samples were collected from 301 food handlers and transported to Adigrat University Microbiology Laboratory. Bacterial isolation and antimicrobial susceptibility test were performed using standard bacteriological methods. Data analysis was performed using SPSS version 22 and P < 0.05 where a corresponding 95% confidence interval was considered statistically significant.Results: A total of 301 food handlers were included in this study. The majority of study participants were females 265 (88.0 %). About 22 (7.3%) and 11 (3.7%) of food handlers were found to be positive for Salmonella and Shigella respectively. Hand washing after using a bathroom with water only, no hand washing after using the bathroom, no hand washing after touching dirty materials, no hand washing before food handling, and untrimmed fingernails were significant associated factors identified. None of the Salmonella and Shigella isolates were sensitive to ampicillin, yet low resistance against chloramphenicol, ceftriaxone, and ciprofloxacin was found. Conclusion: The present study revealed that the prevalence of Salmonella and Shigella among food handlers was 22 (7.3%) and 11 (3.7%) respectively. Such colonized food handlers can contaminate food, and drinks and could serve as a source of infection to consumers. This indicates that there is a need for strengthened infection control measures to prevent Salmonella and Shigella transmission in the students’ cafeteria.

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Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

The Journal of Infection in Developing Countries ◽

10.3855/jidc.7437 ◽

2016 ◽

Vol 10 (04) ◽

pp. 337-346 ◽

Cited By ~ 9

Author(s):

Ahmed Mohamed Ammar ◽

Adel Attia Mohamed ◽

Marwa Ibrahim Abd El-Hamid ◽

Mona Mohamed El-Azzouny

Keyword(s):

Antibiotic Resistance ◽

Foodborne Pathogens ◽

Broiler Chickens ◽

Virulence Gene ◽

Distribution Patterns ◽

Gene Profiling ◽

Virulence Determinants ◽

Resistance Patterns ◽

Microbiological Techniques ◽

Salmonella Serovars

Introduction: Salmonella serovars are one of the primary foodborne pathogens. Poultry consumption is responsible for the majority of disease cases worldwide. The prevalence of virulence determinants among Salmonella serovars appears to be lacking in Egypt. Therefore, this study investigated the occurrence, antibiotic resistance patterns, and virulence gene profiling of Salmonella serovars in broilers. Methodology: Three hundred samples from broiler chickens were examined for the presence of Salmonella by standard microbiological techniques. All Salmonella isolates were tested for their sensitivity against ten antibiotics and subjected to virulence genotyping by polymerase chain reaction (PCR). Results: The overall isolation percentage of Salmonella was 17%. Seven different serovars were found, with the main one being Salmonella Typhimurium (52.94%). Salmonella isolates were sensitive to most of the tested antibiotics, but they exhibited absolute resistance against amoxicillin/clavulanic acid. Nine Salmonella strains (52.94%) were resistant to at least three antibiotics. Further PCR investigations into 17 Salmonella strains revealed different distribution patterns of eight virulence determinants among the isolates. The invA gene was the most prevalent one (100%), followed by hilA (88.24%), stn (58.82%), and fliC genes (52.94%), while each of sopB and pefA genes had a similar prevalence (41.18%), and sefC and spvC genes had the lowest prevalence (11.76 and 5.88%, respectively). PCR genotyping allowed grouping of Salmonella strains into ten genetic profiles. Conclusions: These results will help in understanding the spread of virulence genotypes and antibiotic resistance among Salmonella serovars in broilers.

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Antimicrobial Susceptibility and Genomic Analysis of Aliarcobacter cibarius and Aliarcobacter thereius, Two Rarely Detected Aliarcobacter Species

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.532989 ◽

2021 ◽

Vol 11 ◽

Author(s):

Ingrid Hänel ◽

Eva Müller ◽

Belén González Santamarina ◽

Herbert Tomaso ◽

Helmut Hotzel ◽

...

Keyword(s):

Amino Acid ◽

Foodborne Pathogens ◽

Antimicrobial Susceptibility ◽

Amino Acid Change ◽

Genomic Analysis ◽

Whole Genome ◽

Genome Sequences ◽

Phenotypic Analysis ◽

Phenotypic Resistance ◽

Reference Strains

Aliarcobacter cibarius and Aliarcobacter thereius are two rarely detected Aliarcobacter species. In the study, we analyzed the antimicrobial susceptibility and provide detailed insights into the genotype and phylogeny of both species using whole-genome sequencing. Thermophilic Campylobacter species are the most common bacterial foodborne pathogens causing gastroenteritis in humans worldwide. The genus Aliarcobacter is part of the Campylobacteraceae family and includes the species Aliarcobacter butzleri, Aliarcobacter cryaerophilus, Aliarcobacter skirrowii, and the rarely described Aliarcobacter cibarius, Aliarcobacter faecis, Aliarcobacter lanthieri, Aliarcobacter thereius, and Acrobarter trophiarum. Aliarcobacter are emergent enteropathogens and potential zoonotic agents. Here, we generated, analyzed, and characterized whole-genome sequences of Aliarcobacter cibarius and Aliarcobacter thereius. They were isolated from water poultry farms in Germany, cultured and identified by MALDI-TOF MS. With PCR the identity was verified. Antibiotic susceptibility testing was carried out with erythromycin, ciprofloxacin, doxycycline, tetracycline, gentamicin, streptomycin, ampicillin, and cefotaxime using the gradient strip method (E-test). Whole-genome sequences were generated including those of reference strains. Complete genomes for six selected strains are reported. These provide detailed insights into the genotype. With these, we predicted in silico known AMR genes, virulence-associated genes, and plasmid replicons. Phenotypic analysis of resistance showed differences between the presence of resistance genes and the prediction of phenotypic resistance profiles. In Aliarcobacter butzleri, the nucleotide sequence of the gyrA gene (DQ464331) can show a signature mutation resulting in an amino acid change T85>I. Acrobarter cibarius and Acrobarter thereius showed the same gene as assessed by similarity annotation of the mutations 254C>G. Most of the isolates were found to be sensitive to ciprofloxacin. The ciprofloxacin-resistant Aliarcobacter thereius isolate was associated with the amino acid change T85>I. But this was not predicted with antibiotic resistance databases, before. Ultimately, a phylogenetic analysis was done to facilitate in future outbreak analysis.

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