scholarly journals Comparable Organ Protein Fractional Synthesis Rate of High and Low-Active Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1750-1750
Author(s):  
Kristina Cross ◽  
Jorge Granados ◽  
Gabriella Ten Have ◽  
John Thaden ◽  
J Timothy Lightfoot ◽  
...  
Keyword(s):  
Physical Activity ◽  
Lean Body Mass ◽  
Body Mass ◽  
Physical Activity Level ◽  
Protein Turnover ◽  
Activity Level ◽  
Synthesis Rate ◽  
Activity Regulation ◽  
Fractional Synthesis Rate ◽  
Fractional Synthesis

Abstract Objectives With the rise in physical inactivity and its related diseases, it is necessary to understand the mechanisms involved in physical activity regulation. Scientists have explored physical activity regulation by investigating various physiological mechanisms involving hormones, neurotransmitters, and genetics; however, little is known about the role of metabolism on physical activity level. We hypothesize that protein turnover in specific organs like the muscle is higher in mice previously exhibiting high physical activity levels, as a mechanism to adapt to the increased demand. Therefore, we studied protein fractional synthesis rate (FSR) in tissues of inherently high and low active mice. Methods In order to study protein FSR of various organs, we assessed 12-week-old male inherently low-active (LA) mice (n = 23, lean body mass: 21.0 ± 1.1 g, C3H/HeJ strain) and high active (HA) mice (n = 20, lean body mass: 22.5 ± 1.3, C57L/J strain). One day before tissue collection, a D2O bolus was administered via intraperitoneal injection, and mice were provided D2O enriched drinking water to enrich the total body water to about 5% D2O. Eleven tissues (kidney, heart, lung, muscle, fat, jejunum, ileum, liver, brain, skin, and bone) were collected and analyzed for enrichment of alanine in the intracellular and protein-bound pool (LC-MS/MS). FSR was calculated as -ln(1-enrichment) as fraction per day. Data are mean ± SE (unpaired t-test: GraphPad Prism 8.2). Results We did not find significant differences between protein FSR of HA and LA mice in any measured organ. Example: Protein FSR (fraction/day): muscle (LA: 0.0326±-0.0026, HA: 0.0331 ± 0.0018, P = 0.8673), liver (0.3568 ± 0.0219, 0.3499 ± 0.0217, P = 0.8263), brain (0.0981 ± 0.0056, 0.1041 ± 0.0063, P = 0.4758). Conclusions The observed lack of significant differences in high and low-active mice suggests that differences in specific organ tissue protein turnover may not be a mechanism regulating inherent physical activity level. Since protein turnover is representative of the ability to adapt through upregulation and downregulation of metabolic processes, these results show that high-active mice are inherently no more equipped for metabolic regulation than the low active mice. Funding Sources Sydney and J.L. Huffines Institute for Sports Medicine, Human Performance Student Research Grant and CTRAL Grant.

scholarly journals Protein fractional synthesis rates within tissues of high- and low-active mice

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242926
Author(s):  
Kristina M. Cross ◽  
Jorge Z. Granados ◽  
Gabriella A. M. Ten Have ◽  
John J. Thaden ◽  
Marielle P. K. J. Engelen ◽  
...  
Keyword(s):  
Physical Activity ◽  
Physical Activity Level ◽  
Deuterium Oxide ◽  
Kidney Tissue ◽  
Activity Level ◽  
Metabolic Adaptation ◽  
Synthesis Rate ◽  
Activity Levels ◽  
Activity Regulation ◽  
Fractional Synthesis

With the rise in physical inactivity and its related diseases, it is necessary to understand the mechanisms involved in physical activity regulation. Biological factors regulating physical activity are studied to establish a possible target for improving the physical activity level. However, little is known about the role metabolism plays in physical activity regulation. Therefore, we studied protein fractional synthesis rate (FSR) of multiple organ tissues of 12-week-old male mice that were previously established as inherently low-active (n = 15, C3H/HeJ strain) and high-active (n = 15, C57L/J strain). Total body water of each mouse was enriched to 5% deuterium oxide (D2O) via intraperitoneal injection and maintained with D2O enriched drinking water for about 24 h. Blood samples from the jugular vein and tissues (kidney, heart, lung, muscle, fat, jejunum, ileum, liver, brain, skin, and bone) were collected for enrichment analysis of alanine by LC-MS/MS. Protein FSR was calculated as -ln(1-enrichment). Data are mean±SE as fraction/day (unpaired t-test). Kidney protein FSR in the low-active mice was 7.82% higher than in high-active mice (low-active: 0.1863±0.0018, high-active: 0.1754±0.0028, p = 0.0030). No differences were found in any of the other measured organ tissues. However, all tissues resulted in a generally higher protein FSR in the low-activity mice compared to the high-activity mice (e.g. lung LA: 0.0711±0.0015, HA: 0.0643±0.0020, heart LA: 0.0649± 0.0013 HA: 0.0712±0.0073). Our observations suggest that high-active mice in most organ tissues are no more inherently equipped for metabolic adaptation than low-active mice, but there may be a connection between protein metabolism of kidney tissue and physical activity level. In addition, low-active mice have higher organ-specific baseline protein FSR possibly contributing to the inability to achieve higher physical activity levels.

scholarly journals Analysis of Relationship between the Body Mass Composition and Physical Activity with Body Posture in Children

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Justyna Wyszyńska ◽  
Justyna Podgórska-Bednarz ◽  
Justyna Drzał-Grabiec ◽  
Maciej Rachwał ◽  
Joanna Baran ◽  
...  
Keyword(s):  
Physical Activity ◽  
Body Mass ◽  
Muscle Tissue ◽  
Physical Activity Level ◽  
The Body ◽  
Body Posture ◽  
Activity Level ◽  
Mass Composition ◽  
Body Mass Composition ◽  
The Relationship

Introduction. Excessive body mass in turn may contribute to the development of many health disorders including disorders of musculoskeletal system, which still develops intensively at that time.Aim. The aim of this study was to assess the relationship between children’s body mass composition and body posture. The relationship between physical activity level of children and the parameters characterizing their posture was also evaluated.Material and Methods. 120 school age children between 11 and 13 years were enrolled in the study, including 61 girls and 59 boys. Each study participant had the posture evaluated with the photogrammetric method using the projection moiré phenomenon. Moreover, body mass composition and the level of physical activity were evaluated.Results. Children with the lowest content of muscle tissue showed the highest difference in the height of the inferior angles of the scapulas in the coronal plane. Children with excessive body fat had less slope of the thoracic-lumbar spine, greater difference in the depth of the inferior angles of the scapula, and greater angle of the shoulder line. The individuals with higher level of physical activity have a smaller angle of body inclination.Conclusion. The content of muscle tissue, adipose tissue, and physical activity level determines the variability of the parameter characterizing the body posture.

scholarly journals CORRELATES OF PHYSICAL ACTIVITY IN ADOLESCENTS OF PUBLIC SCHOOLS IN CURITIBA, PARANÁ, BRAZIL

2020 ◽  
Vol 38 ◽  
Author(s):  
Eliane Denise Araújo Bacil ◽  
Thiago Silva Piola ◽  
Michael Pereira da Silva ◽  
Rodrigo Bozza ◽  
Edmar Fantineli ◽  
...  
Keyword(s):  
Physical Activity ◽  
Social Support ◽  
Nutritional Status ◽  
Body Mass ◽  
Physical Activity Level ◽  
Parental Support ◽  
Self Efficacy ◽  
Activity Level ◽  
Biological Maturation ◽  
High Social Support

ABSTRACT Objective: To verify the association of nutritional status, biological maturation, social support and self-efficacy with the physical activity level of 2,347 students of both sexes, aged between 11 and 15 years old, enrolled in state schools in the city of Curitiba, Paraná, Brazil. Methods: Anthropometric measurements of body mass, height and sitting height were collected. The assessment of biological maturation was based on the analysis of the age at peak height and sexual maturity. The physical activity level, social support from parents and friends and self-efficacy were evaluated by self-reported questionnaires. Sex/age-specific body mass index (BMI) cutoff points identified the nutritional status. Gross and adjusted binary logistic regression were used to obtain odds (OR) ratios with 95% confidence intervals (95%CI), adopting p≤0.05 as significant. Results: More than half (52.3%; n=1,227) of students were active, with boys in a higher proportion (64.1%; p≤0.01). The correlates of physical activity were: nutritional status (OR 1.25; 95%CI 1.01-1.56), early somatic maturation (OR 0.71; 95%CI 0.54-0.93), moderate (OR 1.85; 95%CI 1.50-2.30) and high social support from parents (OR 2.70; 95%CI 2.11-3.42) and high social support from friends (OR 1.78; 95%CI 1.42-2.24). Conclusions: Nutritional status, early somatic maturation, social support of parents and friends were correlates of physical activity. Overweight girls with moderate and high parental support and boys with greater social support from parents and friends were more active. Girls with early somatic maturation were less active.

scholarly journals Obesity and Habitual Physical Activity Level among Staffs Working in a Military Hospital in Malacca, Malaysia

2013 ◽  
Vol 12 (1) ◽  
Author(s):  
Assoc. Prof. Dr. Ayiesah Ramli
Keyword(s):  
Physical Activity ◽  
Body Mass Index ◽  
Body Mass ◽  
Physical Activity Level ◽  
Activity Level ◽  
Habitual Physical Activity ◽  
Health Concern ◽  
Military Hospital ◽  
Cross Sectional ◽  
The Mean

Introduction: Obesity has been recognized as a major public health concern due to lack of physical activity and a sedentary lifestyle. Methods: This cross sectional study was carried out to determine the status of body mass index (BMI) and habitual physical activity level among staffs working in Military Hospital, Malacca using Habitual Physical Activity Questionnaire (HPAQ). Results: About 120 HPAQ were distributed and only 82 responded with 23(28%) of them males and 59 (72%) females, aged between 21 to 59 (40.52 ± 9.38) years old. Anthropometry analysis showed the mean weight of male respondents was 79.48 (± 16.33) and 70.95 (± 15.66) for the females. Body mass index (BMI) for males (33.61 ± 2.82) was almost similar to females ( 33.20± 2.95).The mean waist circumference of men was 93.26 (± 10.94)cm compared to 94.03 (± 11.98)cm in the females. Following categorization of BMI, 59% (n=48) are in the obese group, 24% (n=20) are overweight and only 17% (n=14) have an ideal body weight or normal BMI. Relationship between BMI and HPAQ demonstrated strong and significant correlation coefficient for habitual physical activity at work (r = 0.775, p < 0.001), sports (r = 0.735, p < 0.05) and leisure ( r = 0.713, p < 0.001). Conclusion: The staffs working in the Hospital demonstrated poor habitual physical activity which explains for the high incidence of obesity among the staffs. This requires measures to be taken to promote a healthier lifestyle among them.

The isotopic nitrogen turnover rate as a proxy to evaluate in the long-term the protein turnover in growing ruminants

2019 ◽  
Vol 157 (9-10) ◽  
pp. 701-710
Author(s):  
Gonzalo Cantalapiedra-Hijar ◽  
Hélène Fouillet ◽  
Céline Chantelauze ◽  
Nadezda Khodorova ◽  
Lahlou Bahloul ◽  
...  
Keyword(s):  
Protein Turnover ◽  
Plasma Proteins ◽  
Farm Animals ◽  
Synthesis Rate ◽  
Fractional Synthesis Rate ◽  
Non Invasive ◽  
Depletion Rate ◽  
Fractional Synthesis ◽  
Large Farm ◽  
Diet Switch

AbstractProtein turnover is an energy-consuming process that is essential for ensuring the maintenance of living organisms. Gold standard methods for whole-body protein turnover (WBPT) measurement have inherent drawbacks precluding their generalization for large farm animals and use during long periods. Here, we proposed a non-invasive proxy for the WBPT over a long period of time and in a large number of beef cattle. The proxy is based on the rate at which urine-N and plasma proteins are progressively depleted in terms of 15N after a slight decrease in the isotopic N composition of the diet (i.e. diet switch). We aimed to test the ability of this proxy to adequately discriminate the WBPT of 36 growing-fattening young bulls assigned to different dietary treatments known to impact the WBPT rate, with different protein contents (normal v. high) and amino acid profiles (balanced v. unbalanced in methionine). The 15N depletion rate found in plasma proteins represented their fractional synthesis rate, whereas the slow depletion rate found in urine was interpreted as a proxy of the WBPT. The proxy tested in urine suggested different WBPT values between the normal- and high-protein diets but not between the balanced and unbalanced methionine diets. In contrast, the proxy tested in plasma indicated that both dietary conditions affected the fractional synthesis rate of plasma proteins. We considered that the rate at which urine is progressively 15N-depleted following an isotopic diet switch could be proposed as a non-invasive proxy of the WBPT rate in large farm animals.

Sign in / Sign up

Export Citation Format

Share Document