scholarly journals Assessing Calcium and Magnesium Nutritional Status With the Hair Median Derivatives Bioassay

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1063-1063
Author(s):  
Berislav Momčilović ◽  
Juraj Prejac ◽  
Ninoslav Mimica
Keyword(s):  
Nutritional Status ◽  
Human Body ◽  
Biological Indicator ◽  
Saturation Curve ◽  
Reference Ranges ◽  
Linear Segment ◽  
Funding Sources ◽  
Icp Ms ◽  
Metabolic Difference

Abstract Objectives Short-term biological indicator of urinary Mg and Ca excretion helps us to assess human body Mg and Ca nutritional status. In this study, we used the long-term biological indicator tissue of hair to assess Mg and Ca human body nutritional status. Methods Hair Mg and Ca were analyzed in 1073 healthy white adult Caucasians [734 women (♀) and 339 men (♂)] consuming common mid-European diet, with the ICP MS. The log transformed data on hair magnesium and calcium were analyzed with median derivatives bioassay. Results The median values (μg·g−1) were ♀Mg 137.2 and ♂Mg 47.1, and ♀Ca 1721.4 and ♂Ca 492.0, respectively. The linear (adequate) reference ranges of the sigmoid saturation curve for magnesium were ♀Mg 29.7–270.6 and ♂Mg 20.5–90.2, whereas these ranges for calcium were ♀Ca 487.7–4426.8 and ♂Ca 261.1–816.4. The magnesium to calcium (Mg/Ca) ratio in women appears fairly constant along the sigmoid linear segment range (about 0.067), and in difference to the constantly increasing Mg/Ca ratio in men - from 0.078 at the start of the linear segment to 0.096 at the median and 0.1105 before leveling at the top of the curve. Conclusions Women tend to accumulate much more Ca in their hair than men. The results suggest how hair Mg concentration should not be below 29.7 and 20.5 or above 270.6 and 90.2 μg·g−1 in women and men, respectively. Similarly, Ca hair concentrations should be not below 487.7 and 261.1 and higher than 4456.8 and 816.4 in women and men, respectively. The possible health effects of the observed sex related metabolic difference between men and women remains to be elucidated. Funding Sources The authors received no specific funding for this work.

scholarly journals Assessing Human Molybdenum Nutritional Status by Analyzing Frequency distribution Properties in the Hair and Whole Blood with a Median Derivatives Bioassay

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1455-1455
Author(s):  
Berislav Momčilović ◽  
Juraj Prejac ◽  
Ninoslav Mimica
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood ◽  
Biological Indicator ◽  
Linear Range ◽  
Linear Segment ◽  
Cross Sectional ◽  
Funding Sources ◽  
Sigmoid Curve ◽  
Ultra Trace

Abstract Objectives Molybdenum is the essential trace element in human nutrition since it is a part of a pterin coenzyme which is essential for the activity of various oxidases. High doses of Mo are neurotoxic to the human brain. The safe adequate intake of Mo is set at 75–200 µg·g−1, but there is no available long-term biological indicator tissue to assess the Mo nutritional status. The aim of this study was to assess human Mo nutritional status with a median derivatives bioassay. Methods We analyzed frequency distribution of hair Mo (HMo) in a prospective, observational, cross sectional and exploratory epidemiological study of 1073 apparently healthy adults (339 men, M and 734 women, W) from Zagreb, CROATIA. Whole blood Mo (WBMo) was assessed in a population subsample of 143 W and 91 M. Both hair and whole blood, a long- and short-term biological indicator, respectively were analyzed for Mo with the ICP MS at the Center for Biotic Medicine, Moscow, Russia: an ISO certified bioelement (electrolytes, trace- and ultra-trace elements) analytical laboratory. Hair Mo natural frequency distribution was analyzed with the aid of median derivatives bioassay logistic sigmoid curve for men and women separately. The range of values below the linear segment of the sigmoid curve (the adequate Mo intake) was considered to reflect Mo deficiency, whereas the values above that linear range indicate Mo dietary excess. The adequate linear range may be further subdivided into marginal (subclinical), true adequate, and ample region with a 30:60:10 ratio, respectively. Results The linear segment of the hair Mo deposition was distinctly different (ng·g−1) and higher in M (34–75) than W (27–57) with the observed median ♀/♂ ratio 0.82. The marginal (subclinical) interval ranges are M 34–46.3, W 27–36). However, there were no difference in the WBMo confidence intervals between M (455–1380) and W (480–1145); the Median ♀/♂ observed ratio is 0.93. Conclusions Adequate human population Mo nutritional status may be accurately assessed by analyzing hair Mo frequency distribution with the median derivatives bioassay. Apparently, there is gender dependent delay of Mo hair saturation in the men hair. For the first time it was possible to differentiate the subclinical levels of sparsely adequate dietary Mo. Funding Sources Institute for Research and Development of Sustainable Eco Systems, 10,000 Zagreb, Croatia.

scholarly journals Assessing Human Manganese Nutritional Status by Analyzing Its Frequency Distribution Properties in the Hair with a Median Derivatives Bioassay (P10-110-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Berislav Momčilović ◽  
Juraj Prejac ◽  
Ninoslav Mimica
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood ◽  
Biological Indicator ◽  
Linear Range ◽  
Similar Amount ◽  
Linear Segment ◽  
Cross Sectional ◽  
Funding Sources ◽  
Sigmoid Curve

Abstract Objectives Manganese (Mn) is the essential trace element (TE) in human nutrition, but high doses of Mn are toxic to the human mentation and induce brain extrapyramidal system. The main source of manganese is our diet, but there no availale long-term biological indicator for assessing the Mn nutritional status.The aim of this study was to assess human manganese nutritional status by assessing its frequency distribution in the hair with a median derivatives bioassay. Methods In this prospective, observational, cross-sectional, exploratory, and epidemiological study we have analyzed hair Mn (HMn) in 1073 apparently healthy adults (339 men, M and 734 women, W) from Zagreb, Croatia. Whole blood manganese (WBMn) was assessed in a subsample of this population in 143 Women and 91 Men. Both hair and whole blood (short-term biological indicator) samples were analyzed for iron with the ICP MS at the Center for Biotic Medicine, Moscow, Russia: an ISO certified bioelements analytical laboratory. Hair Mn natural frequency distribution was analyzed with the median derivatives bioassay logistic sigmoid curve for men and women separately. The range of values below the linear segment of the sigmoid curve (adequate intake) was considered to reflect Mn deficiency, whereas values above that linear range indicate Mn dietary excess. Results Women and men have almost identical amount of HMn (median ♂0.234 µg·g-1 vs. median ♀ 0.208 µg·g-1). The physiologically adequate HMn linear segment range of the sigmoid curve was 0.090–0.749 µg·g-1 for Men and 0.091–0.628 µg·g-1 for Women. Similarly, both women and men have very similar amount of WBMn (median ♂0.0109 µg∙g-1 vs median ♀0.0118 µg∙g-1). The physiologically adequate WBMn linear range of the sigmoid curve was 0.0084–0.0169 µg∙g-1 for ♀ and 0.0079–0.0179 µg∙g-1 for ♂. Conclusions Human nutritional status of Mn in the population may be reliably assessed by analyzing its frequency distribution in the hair with the median derivatives bioassay. Our exploration indicates how it is possible to diagnose the subclinical states of both manganese nutritional deficiency and excess. When the Mn nutritional status is expressed in a range format it became possible to study simultaneously the subtle and complex multi-bioelement profile interactions within the adequate range of a bioelement intake. Funding Sources Institute for Research and Development of Sustainable Ecosystems, Zagreb, Croatia.

scholarly journals Assessing Human Iron Nutritional Status by Analyzing Its Frequency Distribution Properties in the Hair with a Median Derivatives Bioassay (P10-109-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Berislav Momčilović ◽  
Juraj Prejac ◽  
Ninoslav Mimica
Keyword(s):  
Nutritional Status ◽  
Frequency Distribution ◽  
Whole Blood ◽  
Therapeutic Option ◽  
Human Nutrition ◽  
Similar Amount ◽  
Linear Segment ◽  
Cross Sectional ◽  
Funding Sources ◽  
Sigmoid Curve

Abstract Objectives Iron is the essential trace element (TE) in human nutrition, but also the most frequently observed deficient element in the human nutrition. The aim of this study was to assess human iron nutritional status by assessing its frequency distribution in the hair with a median derivatives bioassay. Methods In this prospective, observational, cross-sectional, exploratory, and epidemiological study we have analyzed hair Fe (HFe) in 1073 apparently healthy adults (339 men M and, 734 women, W) from Zagreb, Croatia. Whole blood iron (WBFe) was assessed in a subsample of this population in 143 Women and 91 Men. Both hair and whole blood samples were analyzed for iron with the ICP MS at the Center for Biotic Medicine, Moscow, Russia. Hair Fe natural frequency distribution was analyzed with the median derivatives bioassay logistic sigmoid curve for men and women separately. The range of values below the linear segment of the sigmoid curve (adequate intake) was considered to reflect Fe deficiency, whereas values above that range indicate Fe dietary excess. Results Women and men have almost identical amount of HFe (median ♂12.72 µg·g-1 vs median ♀ 12.428 µg·g-1). The physiologically adequate HFe linear segment range of the sigmoid curve was 6.09–32.19 µg·g-1 for Men and 6.11 - 28.23 µg·g-1 for Women. Similarly, both women and men have very similar amount of WBFe (median ♂535.6 µg∙g-1 vs median ♀4880 µg∙g-1). The physiologically adequate WBFe linear range of the sigmoid curve was 386.4–606.1 µg∙g-1 for ♀ and 470.4–631.0 µg∙g-1 for ♂. Conclusions Human nutritional status of Fe may be reliably assessed by analyzing its frequency distribution in the hair with median derivatives bioassay. Hair iron is a rare case between the bioelements we have tested thusafar where wholeblood concentrations were higher than that in the hair. Our exploration indicates that the so called iron subclinical deficiency may be adequately quantified. Indeed, continuous subliminal iron supplementation may be a better therapeutic option than current supplementation with high doses associated with the unwanted side effects. Funding Sources Institute for Research and Development of Sustainable Ecosystems, Zagreb, Croatia.

scholarly journals Hair as a Novel Long Term Biological Indicator for Assessing the Human Na and K Nutritional Status

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Berislav Momcilovic ◽  
Juraj Prejac ◽  
Vjeran Višnjević ◽  
Ninoslav Mimica ◽  
Anatoly Skalny
Keyword(s):  
Nutritional Status ◽  
Biological Indicator

Hair for a long-term biological indicator tissue for assessing the strontium nutritional status of men and women

2017 ◽  
Vol 42 ◽  
pp. 11-17 ◽  
Author(s):  
J. Prejac ◽  
V. Višnjević ◽  
A.A. Skalny ◽  
A.R. Grabeklis ◽  
N. Mimica ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Biological Indicator ◽  
Men And Women

scholarly journals Flavonoids Ameliorate Stress-Induced Depression by Preventing NLRP3 Inflammasome Priming

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1231-1231
Author(s):  
Giulio Pasinetti
Keyword(s):  
Mrna Expression ◽  
Chronic Stress ◽  
Nlrp3 Inflammasome ◽  
Signaling Cascades ◽  
Dietary Polyphenols ◽  
Funding Sources ◽  
Glycation End Products ◽  
Molecular Patterns

Abstract Objectives Chronic stress activates danger-associated molecular patterns (DAMPs), stimulating the NLRP3 inflammasome. NLRP3 activation triggers the release of pro-inflammatory cytokine IL-1β. The activity of the NLRP3 inflammasome propagates pro-inflammatory signaling cascades implicated in the onset of depression. Our previous studies show that polyphenolic compounds were found to ameliorate stress induced depression in mouse models. However, the relevant mechanism has not been identified. This study examined the effect of administering polyphenols on DAMP signaling in enriched mice microglia. Methods This study examined the effect of administering polyphenols on DAMP signaling in mice microglia. To recapitulate stress-induced depression, mice underwent chronic unpredictable stress (CUS). Microglia were isolated at various time points throughout the CUS protocol. We also assessed long-term persistent changes after CUS and susceptibility to subthreshold unpredictable stress (US) re-exposure. Results Interestingly, the development of US – induced depression and anxiety depended upon a previous exposure to CUS. We found that CUS caused robust upregulation of IL-1β mRNA in enriched microglia, an effect that persists for up to 4 weeks following CUS exposure. Following the subthreshold US re-exposure, we observed the upregulation of pro- IL-1β as well as pro-receptor for advanced glycation end products (RAGE). Toll-like receptor 4 (TLR-4) was not. We also observed an increase in RAGE mRNA expression when mice were exposed to US prior to the start of the CUS paradigm. Importantly, a primary exposure to US, was sufficient to increase RAGE mRNA expression. We found that polyphenol administration significantly improved CUS-induced depressive-like phenotypes and also reversed neuroinflammation in mice. Treatment with dietary flavonoids prevented upregulation of IL-1β, RAGE mRNA, which reflects the ability of polyphenols that may have begun following the primary exposure to US. Conclusions Taken all together, the results provide evidence of the role of dietary polyphenols in preventing persistent microglial activation, which has been shown to result in reduced long term vulnerability to depressive-like behaviors following expose to chronic stress. Funding Sources This study was supported by a P50 CARBON Center grant from the NCCIH/ODS.

scholarly journals Hair Cortisol Concentration as a Biomarker of Sleep Quality and Related Disorders

Life ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 81
Author(s):  
Nisrin El Mlili ◽  
Hanan Ahabrach ◽  
Omar Cauli
Keyword(s):  
Sleep Disorders ◽  
Reference Ranges ◽  
Hair Cortisol ◽  
Neuropsychiatric Diseases ◽  
Close Relationship ◽  
Adults And Children ◽  
Experimental Approaches ◽  
Common Manifestation ◽  
Functional Alteration

Cortisol is the end product of the hypothalamic-pituitary-adrenal (HPA) axis, and its production is increased mainly in stressful situations or in chronic disorders accompanied by stress enhancement. Altered cortisol concentrations have been reported in a number of neuropsychiatric diseases and sleep disorders. Cortisol concentrations have been measured using several methods, and in several matrixes, such as blood, saliva, and urine. However, lately, hair cortisol, for several reasons, has emerged as a promising biomarker of long-term retrospective HPA activation. Several experimental approaches for cortisol measurement with the corresponding concentration reference ranges and a summary of findings from scientific literature on this field are presented. There is evidence of a close relationship between HPA functional alteration and the development of neuropsychiatric disorders. Sleep disorders are the most common manifestation in several neuropsychiatric conditions, and have also been associated to cortisol alterations in both adults and children. Many studies indicate that hair cortisol constitutes a valuable tool for further contributing to existing data on salivary, plasma, or urinary cortisol concentrations in patients with sleep disorders.

scholarly journals Nutritional Status in Spanish Adults with Celiac Disease Following a Long-Term Gluten-Free Diet Is Similar to Non-Celiac

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1626
Author(s):  
Catalina Ballestero-Fernández ◽  
Gregorio Varela-Moreiras ◽  
Natalia Úbeda ◽  
Elena Alonso-Aperte
Keyword(s):  
Bone Mineral Density ◽  
Vitamin D ◽  
Celiac Disease ◽  
Vitamin E ◽  
Nutritional Status ◽  
Bone Mineral ◽  
Biochemical Parameters ◽  
Gluten Free ◽  
Mineral Density

The only available treatment for celiac disease is life-long gluten exclusion. We conducted a cross-sectional age- and gender-matched study in 64 celiac adults on a long-term (>1 year) gluten-free diet and 74 non-celiac volunteers from Spain, using dietary, anthropometric, and biochemical parameters, as well as assessing bone mineral density and physical activity. Celiac adults had deficient intake (below 2/3 of the recommended intake) for folates, vitamin E, and iodine and low intake of calcium (below 80% of the recommended intake). Iron intake was also below 2/3 of the recommended intake in celiac women. Vitamin D intake was extremely low, and 34% of celiac patients had moderately deficient plasma levels. According to bone mineral density, celiac women may be more prone to osteopenia and osteoporosis. However, we found a perfectly analogous nutritional status scenario in celiac as compared to healthy volunteers, with the dietary deviations found being similar to those of the Spanish population, i.e., both groups followed a high-lipid, high-protein, and low-carbohydrate diet. Values for biochemical parameters were found within the reference ranges. Celiac disease had no influence on body weight, but body fat in celiac patients tended to be higher. According to our results, vitamin D, calcium, folates, vitamin E, iodine, and iron nutritional status should be specifically assessed and monitored in the celiac population.

Child Money and Food Stamps: A comparative analysis of Mongolian welfare programmes in the Ger Districts of Ulaanbaatar

2020 ◽  
Vol 66 (4) ◽  
pp. 499-524
Author(s):  
Elizabeth Fox
Keyword(s):  
Participant Observation ◽  
Food Stamps ◽  
Means Testing ◽  
Economic Cycles ◽  
Funding Sources ◽  
Urban Migrants ◽  
Multi Level ◽  
Welfare Model ◽  
Level Analysis

Abstract Until recently, the Mongolian welfare system was entirely category based. However, a new food stamps programme funded by loans from the Asian Development Bank, which targets aid according to proxy means testing, has been introduced as part of the bank’s aim to push Mongolia towards a fiscally sustainable welfare model. The food stamps programme is presented as efficient and responsible in contrast to Mongolia’s universal child money programme. Based on long-term participant observation research in the ger districts of Ulaanbaatar, areas inhabited by many rural-urban migrants living in poverty, this paper compares the two programmes, interweaving street-level accounts of the experiences of residents and bureaucrats alike with the respective histories and funding sources of the two programmes. Doing so provides a multi-level analysis of the emergent welfare state in Mongolia, unpicking the ‘system’ that ger district residents encounter, linking the relative influence of international financial institutions to democratic and economic cycles, and offering a critique of the supposed efficiency of targeted welfare programmes.

scholarly journals Impact of Comorbidities, Sarcopenia, and Nutritional Status on the Long-Term Outcomes after Endoscopic Submucosal Dissection for Early Gastric Cancer in Elderly Patients Aged ≥ 80 Years

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3598
Author(s):  
Ga Hee Kim ◽  
Kee Don Choi ◽  
Yousun Ko ◽  
Taeyong Park ◽  
Kyung Won Kim ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Overall Survival ◽  
Early Gastric Cancer ◽  
Nutritional Status ◽  
Elderly Patients ◽  
Endoscopic Submucosal Dissection ◽  
Term Survival ◽  
Factors Associated ◽  
Submucosal Dissection

Background/Aim: We investigated the oncologic outcomes in elderly patients who underwent endoscopic submucosal dissection (ESD) for early gastric cancer (EGC) by focusing on the impact of comorbidities, sarcopenia, and nutritional status. Methods: Between 2005 and 2016, 280 patients aged ≥ 80 years with 289 EGCs underwent ESD at a tertiary care center. The short- and long-term survival outcomes were assessed. Cox regression analysis was used to identify factors associated with survival, including clinicopathologic factors and abdominal muscle area measured by computed tomography. Results: The rates of en bloc, R0, and, curative resection were 99.3%, 90.0%, and 69.2%, respectively. The rates of post-ESD bleeding and perforation rates were 2.1% and 3.1%, respectively, and no cases showed significant life-threatening adverse events. Over a median follow-up period of 70.5 months, the 3- and 5-year overall survival (OS) rates were 89.5% and 77.1%, respectively; of the114 patients who died, only four (3.5%) were due to gastric cancer. A total of 173 (61.8%) had sarcopenia, and they had lower rates of 3-year (88.4% vs. 91.4%) and 5-year (73.1% vs. 84.0%; p = 0.046) OS than did those without sarcopenia. In multivariable analyses, prognostic nutritional index (hazard ratio [HR], 0.93; 95% confidence interval [CI]: 0.90–0.98; p = 0.002) and Charlson comorbidity index (HR 1.19; 95% CI: 1.03–1.37; p = 0.018) were significant factors associated with overall survival. Conclusions: ESD was a feasible and safe therapeutic method to use in elderly patients, whose long-term survival was significantly associated with nutritional status and comorbidities. These results suggest the need for a possible extension of the curative criteria for ESD in elderly patients with EGC.

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