Modified Automated Determination of 2,3-Diphosphoglycerate in Whole Blood

1972 ◽  
Vol 18 (9) ◽  
pp. 1001-1004 ◽  
Author(s):  
Kenneth F Atkinson
Keyword(s):  
Sample Size ◽  
Coefficient Of Variation ◽  
Whole Blood ◽  
Normal Range ◽  
Blood Samples ◽  
Automated Determination

Abstract A modification is described of the automated determination of 2,3-diphosphoglycerate (DPG) in blood [Grisolia, S., et al., Anal. Biochem. 31, 235 (1969)]. Modifications in the manifold result in a sensitive, noise-free, rapid system and the modifications in the preparations of the reagents ensure stability of the diluted standards and blood samples for at least three weeks. Samples are run at the rate of 60/h and sample size can be as small as 5 µl of whole blood. The coefficient of variation of the overall determination of automated DPG and manual hemoglobin is 3.6% and the SD is ±0.77 µmol/g Hb. The normal range is 14.6 ± 2.2 (SD) µmol/g hemoglobin.

scholarly journals Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 78
Author(s):  
Sevasti Karampela ◽  
Jessica Smith ◽  
Irene Panderi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Whole Blood ◽  
High Performance ◽  
Tandem Mass ◽  
Psychoactive Substances ◽  
Blood Samples ◽  
Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

scholarly journals Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Mustafa Karapirli ◽  
Murat Kizilgun ◽  
Ozgur Yesilyurt ◽  
Husamettin Gul ◽  
Zeki Ilker Kunak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Simultaneous Determination ◽  
Cyclosporine A ◽  
Whole Blood ◽  
Immunosuppressive Drugs ◽  
Tandem Mass ◽  
Blood Samples ◽  
Whole Blood Samples

Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples.Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients.Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes.Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs.

STUDIES ON FOLIC ACID IN INFANCY

PEDIATRICS ◽  
1964 ◽  
Vol 33 (5) ◽  
pp. 694-699
Author(s):  
Yehuda Matoth ◽  
Rina Zamir ◽  
Shulamith Bar-Shani ◽  
Nathan Grossowicz
Keyword(s):  
Bone Marrow ◽  
Folic Acid ◽  
Folinic Acid ◽  
Whole Blood ◽  
Blood Picture ◽  
Normal Range ◽  
Acid Status ◽  
Effect Of Treatment ◽  
Low Levels

Folic acid was assayed microbiologically in whole blood in a group of infants hospitalized for diarrhea, various infections, and malnutrition. Folic acid activity was decreased in the majority of cases. In some of the infants very low levels were observed. The value of the determination of folic acid in whole blood as a sensitive index of the folic acid status of an individual was confirmed by parallel observations on bone marrow morphology and the level of folic acid in serum. Folinic acid levels were within the normal range in most cases. Low folinic acid levels were common only when folic acid was extremely low. Many patients with low folic acid levels were not anemic, or mildly anemic. In the anemic patients a hypochromic microcytic blood picture was the rule. The effect of treatment with folic acid on the general condition of the patients was more striking than the hematological response.

Development and validation of a dried blood spot–HPLC assay for the determination of metronidazole in neonatal whole blood samples

2010 ◽  
Vol 397 (2) ◽  
pp. 687-693 ◽  
Author(s):  
Maysa Faisal Suyagh ◽  
Godwill Iheagwaram ◽  
Prashant Laxman Kole ◽  
Jeff Millership ◽  
Paul Collier ◽  
...  
Keyword(s):  
Whole Blood ◽  
Dried Blood Spot ◽  
Hplc Assay ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Development And Validation ◽  
Blood Spot

A Method for the Analysis of Total Nitrogen in Natural Waters

1984 ◽  
Vol 41 (5) ◽  
pp. 815-819 ◽  
Author(s):  
J. Kalff ◽  
E. Bentzen
Keyword(s):  
Sample Size ◽  
Total Nitrogen ◽  
Coefficient Of Variation ◽  
Natural Waters ◽  
Sodium Salicylate ◽  
Persulfate Oxidation ◽  
Dissolved Nitrogen

This method for analyzing total nitrogen (TN) in freshwaters is based on the persulfate oxidation of nitrogen to nitrate, followed by the analysis of this nitrate by a modified version of the sodium salicylate method. The method is simpler than other reported techniques for TN in oligotrophy and mesotrophic waters and requires equipment readily available in most laboratories. The method is linear to 1000 μg N/L, with the range extendable by changing the sample size. The variability is lowest (coefficient of variation 6.6%) between 100 and 1000 μg N/L. We have successfully used the method for the determination of TN, as well as dissolved nitrogen (DN) on filtered samples and nitrate on nonoxidized samples.

scholarly journals Detection of hepatitis B surface antigen in whole blood by coupled particle light scattering (Copalis™)

1997 ◽  
Vol 43 (9) ◽  
pp. 1764-1770 ◽  
Author(s):  
Michael J Benecky ◽  
Diane R Post ◽  
Susan M Schmitt ◽  
Manish S Kochar
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Whole Blood ◽  
Direct Detection ◽  
Hepatitis B Surface Antigen ◽  
Light Scatter ◽  
Blood Samples ◽  
Cell Components ◽  
Multiple Analytes

Abstract Coupled particlelight scattering (Copalis™) is a homogeneous immunoassay technology that permits simultaneous determination of multiple analytes in serum, plasma, or whole blood. Copalis differentiates monomeric latex microparticles from latex aggregates and cells on the basis of their unique light scatter properties. Copalis readily discriminates small (∼0.1 μm) differences in latex microparticle size. Therefore, multiple simultaneous assays are configured by the use of mixtures of different-size latex microparticles. The Copalis research immunoassay for hepatitis B surface antigen (HBsAg) is configured in a sandwich format where the extent of light scatter histogram broadening due to HBsAg-mediated binding of colloidal gold to latex provides the basis for antigen quantification. Simultaneous Copalis forward- and wide-angle light scatter measurements allow discrimination of latex microparticles from the cell components of whole blood. Consequently, direct detection of HBsAg in unprocessed whole-blood samples by Copalis is feasible.

Gas-chromatographic/mass-spectrometric determination of theophylline in whole blood.

1977 ◽  
Vol 23 (1) ◽  
pp. 64-68 ◽  
Author(s):  
M Sheehan ◽  
R H Hertel ◽  
C T Kelly
Keyword(s):  
Coefficient Of Variation ◽  
Whole Blood ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Mass Spectrometric Determination ◽  
Pediatric Populations ◽  
Chromatographic Mass

Abstract We describe a combined gas-chromatographic mass-spectrometric method for determination of theophylline in whole blood. With use of a probability-based matching approach, the extensive gas-chromatographic interference did not affect mass-spectrometric quantitation of theophylline. Concentrations expected and found were linearly related from 1 to 60 mg/liter; the run-to-run coefficient of variation was less than 8%, within-run less than 6%. Some other xan-thines and drugs frequently co-administered with theophylline did not affect results. Only 0.2 ml of blood is required, which makes the procedure useful for monitoring theophylline in newborns and pediatric populations.

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