Cholesterol screening: comparative evaluation of on-site and laboratory-based measurements

1990 ◽  
Vol 36 (2) ◽  
pp. 255-260 ◽  
Author(s):  
P S Bachorik ◽  
R Rock ◽  
T Cloey ◽  
E Treciak ◽  
D Becker ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Clinical Laboratory ◽  
Screening Program ◽  
Venous Blood ◽  
Capillary Blood ◽  
Laboratory Measurements ◽  
Community Screening ◽  
Blood Samples ◽  
Cholesterol Screening ◽  
Laboratory Values

Abstract We measured cholesterol in capillary blood samples from 9683 volunteers over a four-day on-site community screening program, using the "Reflotron" desk-top analyzer (Boehringer-Mannheim Diagnostics, Indianapolis, IN). We also measured cholesterol in venous blood samples from 3% of those screened (a) with the Reflotron at the screening sites, (b) in a qualified hospital clinical laboratory, and (c) in a Centers for Disease Control standardized lipoprotein research laboratory. The sensitivity (and specificity) of the Reflotron measurements, with use of the lipoprotein laboratory measurements as the point of reference, was 0.95 (0.73) in capillary blood samples and 0.88 (0.93) in venous blood samples, compared with 0.99 (0.87) in the hospital clinical laboratory. The Reflotron measurements correlated less well with the lipoprotein laboratory values in both venous blood (r = 0.91) and capillary blood (r = 0.89) samples than did the clinical laboratory values (r greater than 0.99). Furthermore, the capillary blood measurements averaged 7% higher than venous measurements when both kinds of samples were analyzed in the Reflotron.

Accuracy and precision of analyses for total cholesterol as measured with the Reflotron cholesterol method.

1989 ◽  
Vol 35 (8) ◽  
pp. 1734-1739 ◽  
Author(s):  
P S Bachorik ◽  
R H Bradford ◽  
T Cole ◽  
I Frantz ◽  
A M Gotto ◽  
...  
Keyword(s):  
Plasma Cholesterol ◽  
Venous Blood ◽  
Reference Method ◽  
Capillary Blood ◽  
Average Difference ◽  
Blood Samples ◽  
Laboratory Values ◽  
Accuracy And Precision ◽  
Single Finger ◽  
Made In

Abstract We compared plasma cholesterol measurements made with the Boehringer Mannheim Reflotron reflectance photometric analyzer in 1298 capillary blood samples with measurements made in venous blood samples collected at the same time and analyzed in four standardized Lipid Research Clinics laboratories. The Reflotron measurements averaged 0.8% to 7.8% lower than the laboratory values. Correlations (r) between the two sets of measurements ranged from 0.92 to 0.96. In some samples, however, the Reflotron values differed from the laboratory values by greater than or equal to 12%; the cholesterol concentrations in these samples tended to be higher than in those for which better agreement was observed. The smaller negative biases were observed when test strips were used that were calibrated with reference to the Centers for Disease Control Reference Method for cholesterol. The agreement between sequential Reflotron values averaged less than or equal to 4.3%. There was an average difference of less than or equal to 1.0% between Reflotron measurements made in each of two sequential capillary blood samples taken from a single finger puncture.

scholarly journals Self-collection of capillary blood using Tasso-SST devices for Anti-SARS-CoV-2 IgG antibody testing

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0255841
Author(s):  
Tess Hendelman ◽  
Anu Chaudhary ◽  
Angela C. LeClair ◽  
Kimberly van Leuven ◽  
Jacqueline Chee ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Venous Blood ◽  
Epidemiologic Studies ◽  
Capillary Blood ◽  
Blood Collection ◽  
Igg Antibodies ◽  
Antibody Testing ◽  
Igg Antibody ◽  
Blood Samples ◽  
Deming Regression

Background Efforts to minimize COVID-19 exposure during the current SARS-CoV-2 pandemic have led to limitations in access to medical care and testing. The Tasso-SST kit includes all of the components necessary for remote, capillary blood self-collection. In this study, we sought to investigate the accuracy and reliability of the Tasso-SST device as a self-collection device for measurement of SARS-CoV-2 IgG antibodies. Methods Capillary blood was obtained via unsupervised and supervised application of the Tasso-SST device, and venous blood was collected by standard venipuncture. Unsupervised self-collected blood samples underwent either extreme summer or winter-simulated shipping conditions prior to testing. Sera obtained by all three methods were tested concurrently using the EuroImmun anti-SARS-CoV-2 S1 IgG assay in a CLIA-certified clinical laboratory. Results Successful Tasso-SST capillary blood collection by unsupervised and supervised administration was completed by 93.4% and 94.5% of participants, respectively. Sera from 56 participants, 55 with documented (PCR+) COVID-19, and 33 healthy controls were then tested for anti-SARS-CoV-2 IgG antibodies. Compared to venous blood results, Tasso-SST-collected (unstressed) and the summer- and winter-stressed blood samples demonstrated Deming regression slopes of 1.00 (95% CI: 0.99–1.02), 1.00 (95% CI: 0.98–1.01), and 0.99 (95% CI: 0.97–1.01), respectively, with an overall accuracy of 98.9%. Conclusions Capillary blood self-collection using the Tasso-SST device had a high success rate. Moreover, excellent concordance was found for anti-SARS-CoV-2 IgG results between Tasso-SST capillary and standard venous blood-derived sera. The Tasso-SST device should enable widespread collection of capillary blood for testing without medical supervision, facilitating epidemiologic studies.

scholarly journals Comparison of capillary, venous and buffy coat blood samples in detecting Plasmodium species among malaria suspected patients attending at Hamusite health center. A cross-sectional study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Getu Abeje ◽  
Woyneshet Gelaye ◽  
Getaneh Alemu
Keyword(s):  
Health Center ◽  
Detection Rate ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Buffy Coat ◽  
Blood Film ◽  
Capillary Blood ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples

Abstract Background Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodium parasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. Methods A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. Results Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. Conclusion Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.

Effects of Different Methods Used to Take Blood Samples on Blood Glucose Measurements

2021 ◽  
pp. 105477382110247
Author(s):  
Eda Ergin ◽  
Ayten Zaybak
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Venous Blood ◽  
Tolerance Test ◽  
Capillary Blood ◽  
Oral Glucose ◽  
Blood Samples ◽  
Significant Difference ◽  
Measurement Results ◽  
Quasi Experimental

The purpose of this study is to compare whether or not there is a difference between venous and capillary blood samples in blood glucose measurements and investigate the effects of different aseptic methods used in skin cleaning before collecting blood samples on measurement results. This quasi-experimental study was conducted with 109 patients. The capillary first and second blood drop values taken from the patients after fasting and at 2 hours following 75 g oral glucose tolerance test (OGTT) and capillary and venous blood glucose values were compared. There was no significant difference between the median venous blood glucose value and the capillary second blood drop value taken after wiping the finger with alcohol. There was no significant difference between the first and second blood drop values of capillary blood glucose 2 hours after OGTT.

scholarly journals Blood Glucose Assay Using Dextrostix and a Reflectance Meter

1972 ◽  
Vol 9 (1-6) ◽  
pp. 91-94 ◽  
Author(s):  
I. W. Percy-Robb ◽  
R. S. McMaster ◽  
A. D. B. Harrower ◽  
L. J. P. Duncan
Keyword(s):  
Blood Glucose ◽  
Venous Blood ◽  
Reference Method ◽  
Capillary Blood ◽  
Blood Samples ◽  
Laboratory Staff ◽  
Glucose Assay ◽  
Reflectance Meter ◽  
High Degree ◽  
Blood Glucose Concentrations

The ‘Dextrostix’-reflectance meter system for blood glucose analysis has been evaluated using a blood glucose reference method. A high degree of concordance between the two methods was obtained when analyses were performed by skilled laboratory staff on venous blood samples containing fluoride, with a 75 s contact time. Skilled laboratory staff performed significantly better than unskilled staff. Capillary blood glucose concentrations correlated poorly with concentrations in venous blood samples taken at the same time as the capillary blood.

scholarly journals Use of Capillary Blood Samples Leads to Higher Parasitemia Estimates and Higher Diagnostic Sensitivity of Microscopic and Molecular Diagnostics of Malaria Than Venous Blood Samples

2018 ◽  
Vol 218 (8) ◽  
pp. 1296-1305 ◽  
Author(s):  
Johannes Mischlinger ◽  
Paul Pitzinger ◽  
Luzia Veletzky ◽  
Mirjam Groger ◽  
Rella Zoleko-Manego ◽  
...  
Keyword(s):  
Molecular Diagnostics ◽  
Venous Blood ◽  
Capillary Blood ◽  
Diagnostic Sensitivity ◽  
Blood Samples

Comparison of capillary blood versus venous blood samples in the assessment of immunity to measles

1996 ◽  
Vol 61 (1-2) ◽  
pp. 73-77 ◽  
Author(s):  
F. Novello ◽  
B. Ridolfi ◽  
L. Fiore ◽  
G. Buttinelli ◽  
E. Medda ◽  
...  
Keyword(s):  
Venous Blood ◽  
Capillary Blood ◽  
Blood Samples

An Assessment of Dried Blood-Spot Technology for Identifying Iron Deficiency

Blood ◽  
1998 ◽  
Vol 92 (5) ◽  
pp. 1807-1813 ◽  
Author(s):  
James D. Cook ◽  
Carol H. Flowers ◽  
Barry S. Skikne
Keyword(s):  
Iron Deficiency ◽  
Transferrin Receptor ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Epidemiologic Studies ◽  
Capillary Blood ◽  
Deficiency Anemia ◽  
Blood Samples ◽  
Blood Spots ◽  
American Society

Abstract The present study was undertaken to assess the feasibility of using ferritin and transferrin receptor measurements on dried capillary blood spots to identify iron deficiency (ID) in public health surveys. Measurements on serum and blood spots prepared from venous blood were performed in 71 healthy subjects, 41 of whom were iron-replete and 30 who had ID, either without (n = 20) or with (n = 10) anemia. Parallel measurements were performed on hemolyzed whole blood and washed hemolyzed red blood cells to assess the erythrocyte contribution of ferritin and transferrin receptor to dried blood samples. The concentration of ferritin in dried blood samples was threefold higher than serum assays due to the release of ferritin from hemolyzed erythrocytes, which diminished the usefulness of ferritin measurements for detecting ID. On the other hand, there was negligible erythrocyte contribution to the measurement of transferrin receptor in dried blood spots. The most sensitive parameter in dried blood spots was the ratio of receptor/ferritin, which was suitable for identifying iron-deficiency anemia (IDA), but less reliable than serum assays for detecting milder ID without anemia. We conclude that tandem measurements of serum ferritin and transferrin receptor in dried blood spots can be used to facilitate the identification of IDA in epidemiologic studies. © 1998 by The American Society of Hematology.

scholarly journals Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples

2020 ◽  
Author(s):  
Margaret A Black ◽  
Guomiao Shen ◽  
Xiaojun Feng ◽  
Wilfredo Garcia Beltran ◽  
Yang Feng ◽  
...  
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Venous Blood ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Antibody Detection ◽  
Lateral Flow Immunoassay ◽  
Blood Samples ◽  
Significant Difference ◽  
Finger Stick

Objectives: Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests. Here, we validate use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and compare results with ELISA. We also seek to establish the value of LFI testing on blood obtained from a capillary blood sample. Methods: Samples collected by venous blood draw and capillary finger stick were obtained from patients with SARS-CoV-2 detected by RT-qPCR and control patients negative for SARS-CoV-2. Samples were tested with the 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) lateral flow immunoassay, and antibody calls were compared with results obtained by ELISA. Results: The Biolidics LFI kit shows clinical sensitivity of 92% at 7 days after PCR diagnosis of SARS-CoV-2 on venous blood. Test specificity was 92% for IgM and 100% for IgG. There was no significant difference in detecting IgM and IgG with Biolidics LFI and ELISA at D0 and D7 (p=1.00), except for detection of IgM at D7 (p=0.04). Finger stick whole blood of SARS-CoV-2 patients showed 93% sensitivity for antibody detection. Conclusions: Clinical performance of Biolidics 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) is comparable to ELISA and showed consistent results across different sample types. Furthermore, we show that capillary blood obtained by finger stick shows similar sensitivity for detecting anti-SARS-CoV-2 IgM and IgG antibodies as venous blood samples. This provides an opportunity for decentralized rapid testing in the community and may allow point-of-care and longitudinal self-testing for the presence of anti-SARS-CoV-2 antibodies.

scholarly journals Ergogenic effect of pre-exercise chicken broth ingestion on a high-intensity cycling time-trial

2021 ◽  
Author(s):  
Silvia Barbaresi ◽  
Laura Blancquaert ◽  
Zoran Nikolovski ◽  
Sarah de Jager ◽  
Mathew Wilson ◽  
...  
Keyword(s):  
High Intensity ◽  
Venous Blood ◽  
Time Trial ◽  
Capillary Blood ◽  
Blood Analysis ◽  
Meat Extract ◽  
Blood Samples ◽  
Cycling Time Trial ◽  
Chicken Broth ◽  
Cycling Time

Abstract Background: chicken meat extract is a popular functional food in Asia. It is rich in the bioactive compounds carnosine and anserine, two histidine-containing dipeptides (HCD). Studies suggest that acute pre-exercise ingestion of chicken extracts has important applications towards exercise performance and fatigue control, but the evidence is equivocal. This study aimed to evaluate the ergogenic potential of the pre-exercise ingestion of a homemade chicken broth (CB) vs a placebo soup on a short-lasting, high-intensity cycling exercise. Methods: fourteen men participated in this double-blind, placebo-controlled, crossover intervention study. Subjects ingested either CB, thereby receiving 46.4 mg/kg body weight of HCD, or a placebo soup (similar in taste without HCD) 40 min before an 8 min cycling time trial (TT) was performed. Venous blood samples were collected at arrival (fasted), before exercise and at 5 min recovery. Plasma HCD were measured with UPLC-MS/MS and glutathione (in red blood cells) was measured through HPLC. Capillary blood samples were collected at different timepoints before and after exercise. Results: a significant improvement (p=0.033; 5.2%) of the 8 min TT mean power was observed after CB supplementation compared to placebo. Post-exercise plasma carnosine (p<0.05) and anserine (p<0.001) was significantly increased after CB supplementation and not following placebo. No significant effect of CB supplementation was observed either on blood glutathione levels, nor on capillary blood analysis. Conclusions: oral CB supplementation improved the 8 min TT performance albeit it did not affect the acid-base balance or oxidative status parameters. Further research should unravel the potential role and mechanisms of HCD, present in CB, in this ergogenic approach.

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