Evaluation of MMP-10 and TIMP-1 levels associated with Resveratrol supplementation

2020 ◽  
Vol 30 (Supplement_2) ◽  
Author(s):  
AM Silva ◽  
C Ferreira ◽  
I Silva ◽  
M Clemente ◽  
JP Figueiredo ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Biochemical Markers ◽  
Red Wine ◽  
Antioxidant Properties ◽  
Intervention Group ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Control Group ◽  
Slot Blot ◽  
Therapeutic Benefits ◽  
Study Population

Abstract Introduction Resveratrol (RSV) is a natural phytoalexin present in abundance in grapes, berries, peanuts and red wine. The main benefits of resveratrol ingestion are due to its anti-inflammatory and antioxidant properties. This compound also induces therapeutic benefits in the remodelling of extracellular matrix (ECM), which plays an important role in the regulation of the activity of matrix metalloproteinases (MMPs) and its tissue inhibitors of metalloproteinases (TIMPs). The MMPs are a family of zinc dependent endopeptidases, with an important role in the physiological and pathological remodelling of ECM, once the activity of MMPs can be regulated by TIMPs. This regulation is essential, since if there is an imbalance between the MMPs and TIMPs there’s destruction of the ECM, leading to the development of several pathologies. Objectives Evaluate RSV potential by determining serum and salivary levels of MMP-10 and TIMP-1. Methodology The study population included 27 undergraduates between the ages of 18 and 30, divided into a control group (placebo) and an intervention group, supplemented with 100 mg RSV/day, during 30 days. MMP-10 and TIMP-1 levels were determined by slot blot. The results were analysed using a GraphPad Prism version 5 software for Windows (GraphPad Software, San Diego, California, USA). Results It was verified the existence of a linear correlation between the serum and salivary levels of TIMP-1, as well as a tendency of increase of this biomarker after RSV supplementation. In all indicators studied, supplementation with this compound wasn’t harmful. Conclusion The rising trend verified at TIMP-1 in both fluids may reflect benefits in the individuals under study, demonstrating that saliva has potential as a study biofluid for this type of biochemical markers. It was also verified that supplementation with this compound did not damage the evaluated parameters and, therefore, the RSV potential shouldn’t be rejected.

scholarly journals Effects of Passive Hydrotherapy WATSU (WaterShiatsu) in the Third Trimester of Pregnancy: Results of a Controlled Pilot Study

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Agnes M. Schitter ◽  
Marko Nedeljkovic ◽  
Heiner Baur ◽  
Johannes Fleckenstein ◽  
Luigi Raio
Keyword(s):  
Pilot Study ◽  
Pregnant Women ◽  
Qualitative Data ◽  
Passive Control ◽  
Intervention Group ◽  
Treatment Method ◽  
Control Group ◽  
Third Trimester ◽  
Therapeutic Benefits ◽  
Negative Side Effects

Background. WATSU (WaterShiatsu) is a complementary therapeutic treatment method comprising passive stretches and massage techniques administered in 35°C warm water. Pregnant women claim safe methods to reduce pain, stress, and fatigue. Therefore, we conducted a pilot study evaluating the effects of WATSU on pregnancy-related complaints in third trimester pregnant women.Methods. Nine healthy pregnant women at gestational week ≥34 were included in an intervention group (receiving WATSU) and compared to eight women in a passive control group (receiving no treatment). WATSU was performed on days 1 and 4 of the study, accompanied by ultrasound examinations. Outcomes include physiological and psychometric as well as qualitative data. Participants in the control group completed questionnaires only.Results. WATSU was found to significantly lower participants’ levels of stress and pain and to improve their mental health-related quality of life and mood. In comparison to the passive control group, participants in the intervention group reported reduction in perceived stress from day 1 to day 8 (P=0.036, Cohen’sf=0.57). Qualitative data indicate that WATSU was appreciated as enjoyable and deeply relaxing. No negative side effects were reported.Conclusion. Our findings support the notion that WATSU yields therapeutic benefits for pregnant women and warrant further research. This study has been registered at ClinicalTrials.gov:NCT01708018.

scholarly journals 4016 Combined Eating Disorder and Weight-Loss Online Guided-Self Help Intervention: Updated Results from an Ongoing Pilot Study

2020 ◽  
Vol 4 (s1) ◽  
pp. 54-54
Author(s):  
Grace Elise Monterubio ◽  
Ellen E. Fitzsimmons-Craft ◽  
Denise E. Wilfley
Keyword(s):  
Eating Disorder ◽  
Pilot Study ◽  
Mixed Model ◽  
Intervention Group ◽  
Baseline Survey ◽  
Control Group ◽  
Student Health Services ◽  
Eating Disorder Examination ◽  
Self Help ◽  
Study Population

OBJECTIVES/GOALS: Aims 1&2: Develop (1) and implement (2) online, guided self-help intervention for ED psychopathology and weight reduction. Aim 3: Follow-up to track remission of ED psychopathology and symptoms and WL maintenance at end of treatment and 6-months. METHODS/STUDY POPULATION: N = 60 college students meeting criteria (clinical/sub-clinical binge-type ED with BMI > 25) will complete a baseline survey and then will be randomized into a condition. Students in the intervention group (n = 30) will be offered 8 weeks of an online, guided self-help intervention for ED and WL. Students in the control group (n = 30) will receive an email message to seek support from Student Health Services. All participants will receive follow-ups 9 weeks and 6 months after baseline. Data analysis will compare Eating Disorder Examination Questionnaire (EDE-Q) scores and WL (change in BMI) at all three time-points. Group comparisons will be assessed via two-way mixed-model ANOVA. RESULTS/ANTICIPATED RESULTS: Recruitment is still ongoing. Data collected by the time of the conference will be presented on the poster. DISCUSSION/SIGNIFICANCE OF IMPACT: Online, guided self-help interventions have been used for WL, as well as for treatment of EDs separately, but no program exists to manage these commonly comorbid conditions concurrently. Thus, this pilot study will examine the effectiveness of combined programs to breach this treatment gap.

scholarly journals A workplace feasibility study of the effect of a minimal fruit intervention on fruit intake

2010 ◽  
Vol 14 (8) ◽  
pp. 1382-1387 ◽  
Author(s):  
Sevil Alinia ◽  
Anne D Lassen ◽  
Kirstine S Krogholm ◽  
Tue Christensen ◽  
Ole H Hels ◽  
...  
Keyword(s):  
Total Energy ◽  
Daily Intake ◽  
Intervention Group ◽  
Normal Weight ◽  
Control Group ◽  
Fruit Intake ◽  
Added Sugar ◽  
Fruit Availability ◽  
Study Population ◽  
Energy And Nutrient Intake

AbstractObjectiveThe main purpose of the study was to investigate the feasibility of using workplaces to increase the fruit consumption of participants by increasing fruit availability and accessibility by a minimal fruit programme. Furthermore, it was investigated whether a potential increase in fruit intake would affect vegetable, total energy and nutrient intake.DesignA 5-month, controlled, workplace study where workplaces were divided into an intervention group (IG) and a control group (CG). At least one piece of free fruit was available per person per day in the IG. Total fruit and dietary intake was assessed, using two 24 h dietary recalls at baseline and at endpoint.SettingEight Danish workplaces were enrolled in the study. Five workplaces were in the IG and three were in the CG.SubjectsOne hundred and twenty-four (IG, n 68; CG, n 56) healthy, mainly normal-weight participants were recruited.ResultsMean daily fruit intake increased significantly from baseline to endpoint only in the IG by 112(se 35) g. In the IG, mean daily intake of added sugar decreased significantly by 10·7(se 4·4) g, whereas mean daily intake of dietary fibre increased significantly by 3·0(se 1·1) g. Vegetable, total energy and macronutrient intake remained unchanged through the intervention period for both groups.ConclusionsThe present study showed that it is feasible to increase the average fruit intake at workplaces by simply increasing fruit availability and accessibility. Increased fruit intake possibly substituted intake of foods containing added sugar. In this study population the increased fruit intake did not affect total energy intake.

scholarly journals The Unwounded Skin Remodeling in Animal Models of Diabetes Types 1 and 2

2013 ◽  
pp. 519-526 ◽  
Author(s):  
M. KNAŚ ◽  
M. NICZYPORUK ◽  
A. ZALEWSKA ◽  
H. CAR
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Diabetes Type ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Control Group ◽  
Diabetes Type 1 ◽  
Tissue Inhibitors ◽  
The Matrix

Diabetes mellitus types 1 and 2 are chronic diseases that cause serious health complications, including dermatologic problems. The diabetic skin is characterized by disturbances in collagen metabolism. A tissue remodeling depends on the degradation of extracellular matrix through the matrix metalloproteinases, which are regulated by e.g. the tissue inhibitors of metalloproteinases. The balance between matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) is essential to maintain homeostasis in the skin. The aim of this study was to determine the concentration of metalloproteinase 2, tissue inhibitor of metalloproteinase 3 and the concentration of collagen type 1 in unwounded skin of diabetes type 1 and 2 and healthy controls. The treatment of diabetes resulted in a significant decrease of MMP2, increase of TIMP3 and COL1 concentrations in the skin as compared to the untreated diabetic skin. The concentrations of MMP2 in the skin of treated rats did not show significant differences from the healthy control group. TIMP3 concentrations in the skin of treated rats are not returned to the level observed in the control group. Disturbances of the extracellular matrix of the skin are similar in diabetes type 1 and 2. Application of insulin in diabetes therapy more preferably affects the extracellular matrix homeostasis of the skin.

scholarly journals Probiotics Effect on Interleukin-4 (IL-4) and Imunoglobulin E (IgE) Levels on Asthmatic Patients

Sains Medika ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 61 ◽  
Author(s):  
Pujiati Pujiati
Keyword(s):  
Serum Level ◽  
Childhood Asthma ◽  
Immunoglobulin E ◽  
Controlled Trial ◽  
Intervention Group ◽  
Serum Levels ◽  
Interleukin 4 ◽  
Control Group ◽  
Asthmatic Patients ◽  
Therapeutic Benefits

Introduction: Probiotics may play a role in immune system maturation and may reduce the risk of allergies and asthma in childhood. However, the therapeutic benefits of probiotics in asthma depend on various factors such as strain of probiotics and dosing regimen. Objectives: The aims of this study was to evaluate the effect of probiotic (LactoB®) on Immunoglobulin E (IgE) and Interleukin (IL-4) serum level in childhood Asthma. Methods: Forty children aged 1-5 years with asthma were recruited into a randomized controlled trial. The children were assigned into a probiotic (Lactobacillus acidophilus, Bifidobacterium longun, Streptococcus; Lacto B®) or an equivalent volume of placebo, twice daily orally for 8 weeks. The IgE and IL-4 serum level were determined by ELISA. The differences between groups were analyzed by t-test dependent with confidence interval of 95%. Results: In intervention group, mean of IgE serum levels after the probiotics treatment was significantly lower compared to that of before the treatment (148.18 pg/mg; 283.20 pg/mg; respectively). Mean of IL-4 serum levels after the treatment was significantly lower compared with that of before the treatment (111.03 pg/mg; 142.08 pg/mg respectively). In control group, there were no significant differences between IgE serum levels mean before the administration of placebo and after the intervention (292.39 pg/ml; 286.94 pg/ml respectively). There were no significant differences between IL-4 serum levels mean before and after the treatment (136.76 pg/ml; 139.56 pg/ml).Conclusion: there was an effect of probiotics supplementation on IgE and IL-4 serum levels in childhood asthma.

Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

Drug Research ◽  
2021 ◽  
Author(s):  
Elnaz Khordad ◽  
Fatemeh Alipour ◽  
Mahdieh Pourabbas ◽  
Somaieh Mansouri ◽  
Ramin Salimnejad
Keyword(s):  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Intervention Group ◽  
The Body ◽  
Control Group ◽  
Male Mice ◽  
Adverse Impacts ◽  
Total Antioxidant ◽  
Group 2 ◽  
Hepatocyte Necrosis

Abstract Background Despite its vast spectrum of clinical usage, cyclophosphamide (CP) exerts many adverse impacts, including hepatotoxicity. Antioxidant properties of ghrelin might protect the liver from CP-induced toxicity. The current study aimed to assess the protective impacts of ghrelin on CP-induced liver toxicity. Methods Forty male mice were randomly divided into four groups (n=10) Group 1 as control received no intervention,group 2 received cyclophosphamide (CP) (100 mg/kg, i.p.) for five weeks and once a week. Group 3 received CP+ghrelin (CP+G), (80 µg/kg daily, i.p.) for five weeks. Group 4 received ghrelin with above-mentioned dose. At the end of the experiment, the mice were sacrificed to remove liver tissuesfor histological and biochemical examination. Results Malondialdehyde (MDA) level increased after CP treatment but ghrelin administration significantly decreased the level of MDA (P<0.05). Measurement of the total antioxidant capacity (TAC) noted a significant decrease in the CP group against the control group (P<0.05). Ghrelin treatment in the CP+G group considerably increased the TAC activity when compared to the CP group (P<0.05). Histological examinations also confirmed the hepatocyte necrosis, local bleeding and inflammation, vacuolation, and sinusoidal dilation in the CP group, ghrelin administration reduced the destructive effects of CP on the liver significantly (P<0.05). Conclusion Our results reveal the hepatoprotective effect of ghrelin against CP. Therefore, ghrelin might be useful in protecting the body against the adverse impacts of injuries induced by chemotherapeutic drugs.

scholarly journals SECONDARY PREVENTION TERHADAP MOTIVASI KLIEN HIPERTENSI DALAM PENGONTROLAN TEKANAN DARAH

2018 ◽  
Vol 1 (1) ◽  
pp. 42
Author(s):  
Yenni Yenni ◽  
Reni Reni
Keyword(s):  
Blood Pressure ◽  
Secondary Prevention ◽  
Statistical Tests ◽  
Intervention Group ◽  
Bivariate Analysis ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Average Value ◽  
Study Population

<p><em>The aims of this study to determine the effect of secondary prevention on the motivation of hypertension clients in controlling blood pressure. Conducted from February to March 2018 in the Neurology Room and Internation of RSUD Prof. Dr. MA. Hanafiah Batusangkar BC Tanah. This type of research was an experiment with a Quasi Experiment Design Nonequivalent Control Group design. The study population was moderate hypertension patients aged 18-59 years, taken by purposive sampling as many as 10 intervention groups and 10 control groups. Bivariate analysis using the Mann-Whitney test. Statistical tests show there is a secondary prevention effect on the motivation of hypertensive clients in controlling blood pressure. p value = 0.012 (p value &lt;0.05). The average value of the motivation of the intervention group was 69.0, SD 8.313,  Mean Rank 13.8 and the control group 58.70, SD 3.683,  Mean Rank 7.20. It can be concluded that there is an effect of the secondary prevention implementation on the motivation of hypertensive clients in controlling blood pressure. It is expected that nurses in hospitals and health centers to be able to use secondary prevention as one strategy in increasing the motivation of clients to control blood pressure.</em></p><p><strong><em>Keywords: motivation, secondary prevention, blood pressure</em></strong></p>

scholarly journals Effect of Square Dance Exercise on Older Women With Mild Mental Disorders

2021 ◽  
Vol 12 ◽  
Author(s):  
Jindong Chang ◽  
Yuping Chen ◽  
Chunzhen Liu ◽  
Liming Yong ◽  
Ming Yang ◽  
...  
Keyword(s):  
Cognitive Impairment ◽  
Mild Cognitive Impairment ◽  
Mental Disorders ◽  
Older Women ◽  
Intervention Group ◽  
Adjunctive Treatment ◽  
Control Group ◽  
Exercise Interventions ◽  
Therapeutic Benefits ◽  
Clinical Experiments

Many epidemiological studies have demonstrated the therapeutic benefits of exercise (EX) that can be used for adjunctive treatment in mental disorders. Despite several clinical experiments using exercise interventions, controlled studies are sparse in most disorder groups. Square dance is a popular aerobic exercise for older women in China. This study aimed to explore the effect of Chinese square dance exercise on mild mental disorders in older women. Participants included 109 older women with mild cognitive impairment from four large nursing homes. Participants were assigned either to the intervention group (n = 62) or the control group (n = 47), according to their residential nursing home. The intervention group underwent an 18-week square dance exercise, while the control group maintained their usual lifestyle. The outcomes were tested at baseline and weeks 9 and 18. The results showed that square dance exercise positively affected the results of all evaluations, especially on the participants' depressive symptoms and quality-of-life-related mental health. This study demonstrates that square dance exercise is a safe and effective approach for older women with mild cognitive impairment that benefits their long-term health.

scholarly journals Matrix metalloproteinases-1, -13 and their tissue inhibitor-1 in endocrine ophthalmopathy

2019 ◽  
Vol 65 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Elizaveta S. Taskina ◽  
Svetlana V. Kharintseva
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Clinical Laboratory ◽  
Thyroid Stimulating Hormone ◽  
Extraocular Muscles ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Control Group ◽  
Clinical Group ◽  
Matrix Remodeling ◽  
Extracellular Matrix Components

Effective regeneration of damaged soft orbital tissues in Graves ophthalmopathy (GO) requires coordinated remodeling of the extracellular matrix. Matrix metalloproteinases (MMPs) play an important role in the synthesis and degradation homeostasis of extracellular matrix components in various physiological and pathological conditions. Their proteolytic activity is inhibited by tissue inhibitors of metalloproteinases (TIMP). The biochemical processes taking place in extraocular muscles and retrobulbar tissue fibrogenesis in GO are not fully understood. Aims to assess some biochemical mechanisms of extraocular muscles and retrobulbar tissue fibrogenesis in GO patients. Material and methods. The study included 65 people (130 eyes) at the age of 43 (3550) years. Three groups of subjects were formed: 32 patients with a moderate GO severity (clinical group), 18 patients with autoimmune thyroid pathology without GO (comparison group), and 15 healthy persons (control). The diagnosis was based on clinical, laboratory, and instrumental data. A comprehensive ophthalmologic examination and blood sampling for determination of MMP-1, -13, TIMP-1, sulfated glycosaminoglycans (sGAG) and antibodies to thyroid-stimulating hormone receptor (TSHRAbs) were conducted. The data were statistically processed using the program Statistica 10.0. Results. An elevated level of MMP-13, observed in all GO patients (p0.05). For the active phase of GOP, the comparison with the control group showed a 3.5-fold increase in MMP-13 (p0.001) and 1.17-fold rise in TIMP-1 (p0.05). Pulse glucocorticoid therapy reduced MMP-13 by 48.6% (p0.001), TIMP-1 by 2.7% (p0.001), and TSHRAbs by 93% (p0.001) compared with active GO, but these indicators were higher than the reference limits of control (p0.05). In inactive GO, despite increased MMP-13, TIMP-1 decreased to the reference values (p=0.533). There were no significant differences in MMP-1 in groups of subjects (p=0.865). Conclusions. We have found imbalance between MMP-13 and TIMP-1 production in different activity phases of GO. Active GO is characterized by an increase in serum MMP-13 and TIMP-1. Dysregulation of intercellular matrix remodeling, possibly, underlies the development of extraocular muscles and retrobulbar tissue fibrosis in GO.

scholarly journals The effect of group logo therapy on the burden of hemodialysis patients’ caregivers

2020 ◽  
Vol 9 (4) ◽  
pp. e33-e33
Author(s):  
seyedeh zahra hoseinigolafshani ◽  
Soraya Taheri ◽  
Maryam Mafi ◽  
Mohamad Hossein Mafi ◽  
Leila Kasirlou
Keyword(s):  
Caregiver Burden ◽  
Intervention Group ◽  
Hemodialysis Patients ◽  
Vital Role ◽  
Control Group ◽  
Effective Care ◽  
Quasi Experimental ◽  
Study Population ◽  
High Level

Introduction: The relationship between caregivers and patients plays a vital role in providing effective care. Objectives: The aim of this study was to investigate the effect of group logo therapy on the burden of hemodialysis patients’ caregivers. Patients and Methods: The present research was a quasi-experimental study with pretest-posttest design. The study population was all caregivers of hemodialysis patients in Qazvin. The sample of this study consisted of 100 caregivers (50 participants in the intervention group and 50 participants in the control group) who were selected according to the inclusion criteria and then randomly allocated in two groups. After the initial evaluation of burden in both groups, 10 sessions, (90 minutes for each session) logo therapy was conducted for intervention group. Immediately after the end of the course and one month later, the intensity of caregiver burden in both groups was measured. The data collection tools were demographic questionnaires for the patient and caregiver and the Zarit Burden Interview. Results: In this study we found, the intervention group with a mean score of 44.6±7.4 and the control group with a mean score of 44.8±5.38 experienced severe burden. Regarding the effect of logo therapy on caregiver burden, the results showed that the mean score of burden before the intervention was 44.6±7.4 and immediately after the intervention and one month later were 37.2±12.6 and 2.14±6.34, respectively (P<0.001). Conclusion: The findings showed the positive effect of group logo therapy on reducing the burden of hemodialysis patients’ caregivers. Considering the high level of caregiver burden, it appears that the application of logo therapy in educational programs for dialysis centers is necessary to reduce the caregiver burden and improve the quality of life.

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