Identification of Genes That Regulate a Left-Right Asymmetric Neuronal Migration inCaenorhabditis elegans

Genetics ◽  
2003 ◽  
Vol 164 (4) ◽  
pp. 1355-1367 ◽  
Author(s):  
QueeLim Ch’ng ◽  
Lisa Williams ◽  
Yung S Lie ◽  
Mary Sym ◽  
Jennifer Whangbo ◽  
...  
Keyword(s):  
Cell Migration ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Neuronal Migration ◽  
Wnt Signaling Pathway ◽  
Hox Gene ◽  
C Elegans ◽  
Asymmetric Response ◽  
Left Right Asymmetry ◽  
Wnt Signal

AbstractIn C. elegans, cells of the QL and QR neuroblast lineages migrate with left-right asymmetry; QL and its descendants migrate posteriorly whereas QR and its descendants migrate anteriorly. One key step in generating this asymmetry is the expression of the Hox gene mab-5 in the QL descendants but not in the QR descendants. This asymmetry appears to be coupled to the asymmetric polarizations and movements of QL and QR as they migrate and relies on an asymmetric response to an EGL-20/Wnt signal. To identify genes involved in these complex layers of regulation and to isolate targets of mab-5 that direct posterior migrations, we screened visually for mutants with cell migration defects in the QL and QR lineages. Here, we describe a set of new mutants (qid-5, qid-6, qid-7, and qid-8) that primarily disrupt the migrations of the QL descendants. Most of these mutants were defective in mab-5 expression in the QL lineage and might identify genes that interact directly or indirectly with the EGL-20/Wnt signaling pathway.

Hox gene expression in a single Caenorhabditis elegans cell is regulated by a caudal homolog and intercellular signals that inhibit wnt signaling

Development ◽  
1999 ◽  
Vol 126 (4) ◽  
pp. 805-814 ◽  
Author(s):  
C.P. Hunter ◽  
J.M. Harris ◽  
J.N. Maloof ◽  
C. Kenyon
Keyword(s):  
Caenorhabditis Elegans ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Postembryonic Development ◽  
Regulatory Pathways ◽  
Hox Gene ◽  
Sensory Ray ◽  
Body Regions ◽  
Anterior Body

In Caenorhabditis elegans males, a row of epidermal precursor cells called seam cells generates a pattern of cuticular alae in anterior body regions and neural sensilla called rays in the posterior. The Hox gene mab-5 is required for two posterior seam cells, V5 and V6, to generate rays. In mab-5 mutant males, V5 and V6 do not generate sensory ray lineages but instead generate lineages that lead to alae. Here we show that two independent regulatory pathways can activate mab-5 expression in the V cells. First, the caudal homolog pal-1 turns on mab-5 in V6 during embryogenesis. Second, a Wnt signaling pathway is capable of activating mab-5 in the V cells during postembryonic development; however, during normal development Wnt signaling is inhibited by signals from neighboring V cells. The inhibition of this Wnt signaling pathway by lateral signals between the V cells limits the number of rays in the animal and also determines the position of the boundary between alae and rays.

A Wnt signaling pathway controls hox gene expression and neuroblast migration in C. elegans

Development ◽  
1999 ◽  
Vol 126 (1) ◽  
pp. 37-49 ◽  
Author(s):  
J.N. Maloof ◽  
J. Whangbo ◽  
J.M. Harris ◽  
G.D. Jongeward ◽  
C. Kenyon
Keyword(s):  
Gene Expression ◽  
Wnt Signaling ◽  
Wnt Pathway ◽  
Hox Genes ◽  
Wnt Signaling Pathway ◽  
Beta Catenin ◽  
Hox Gene ◽  
C Elegans ◽  
Neuroblast Migration ◽  
Pathway Gene

The specification of body pattern along the anteroposterior (A/P) body axis is achieved largely by the actions of conserved clusters of Hox genes. Limiting expression of these genes to localized regional domains and controlling the precise patterns of expression within those domains is critically important for normal patterning. Here we report that egl-20, a C. elegans gene required to activate expression of the Hox gene mab-5 in the migratory neuroblast QL, encodes a member of the Wnt family of secreted glycoproteins. We have found that a second Wnt pathway gene, bar-1, which encodes a beta-catenin/Armadillo-like protein, is also required for activation of mab-5 expression in QL. In addition, we describe the gene pry-1, which is required to limit expression of the Hox genes lin-39, mab-5 and egl-5 to their correct local domains. We find that egl-20, pry-1 and bar-1 all function in a linear genetic pathway with conserved Wnt signaling components, suggesting that a conserved Wnt pathway activates expression of mab-5 in the migratory neuroblast QL. Moreover, we find that members of this Wnt signaling system play a major role in both the general and fine-scale control of Hox gene expression in other cell types along the A/P axis.

scholarly journals CXCL12 mediates T‐cell migration via activation of the non‐canonical Wnt signaling pathway

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Manik C. Ghosh ◽  
Gary D Collins ◽  
Arnell Carter ◽  
Bolormaa Vandanmagsar ◽  
Margaret Brill ◽  
...  
Keyword(s):  
Cell Migration ◽  
T Cell ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Canonical Wnt Signaling ◽  
T Cell Migration ◽  
Canonical Wnt

scholarly journals Advancement of Wnt signal pathway and the target of breast cancer

2016 ◽  
Vol 11 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Quan Liang ◽  
Wei Li ◽  
Zhanchao Zhao ◽  
Qiang Fu
Keyword(s):  
Breast Cancer ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Cancer Metastasis ◽  
Wnt Signaling Pathway ◽  
Signal Pathway ◽  
Bkca Channels ◽  
Wnt Signal Pathway ◽  
Wnt Signal

AbstractWnt/β-catenin signaling has been proved to play an important role in the development and promotion of cancer metastasis. The activation of Wnt signals can lead to duplicating, updating, metastasizing and relapsing. The Wnt signaling pathway is mainly divided into the Wnt/β-catenin pathway and the Wnt/calcium pathway. A better understanding of all the diverse functions of Wnt and their molecular mechanisms has evoked prevailing interest in identifying additional targets related to the Wnt /β-catenin pathways in breast cancer. A number of new target, related to Wnt /β-catenin pathways have been identified in recent years, including NOP14, BKCa channels, Emilin2, WISP, MicroRNAs, NRBP1, TRAF4, and Wntless. In this review, we will introduce the new targets related to the Wnt /β-catenin pathways in breast cancer.

scholarly journals Abstract 4917: Estrogen receptor D538G mutation promotes cell migration via hyperactivation of Wnt signaling pathway

2020 ◽  
Author(s):  
Zheqi Li ◽  
Kevin M. Levine ◽  
Spencer Arneson ◽  
Kristofer C. Berrett ◽  
Nolan M. Priedigkeit ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Cell Migration ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway

scholarly journals Phorbol Esters with Wnt Signal-Augmenting Effects Isolated from Excoecaria Indica

2012 ◽  
Vol 7 (4) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Tatsuhiro Yamaguchi ◽  
Kazufumi Toume ◽  
Midori A. Arai ◽  
Firoj Ahmed ◽  
Samir K. Sadhu ◽  
...  
Keyword(s):  
Natural Products ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Transcriptional Activity ◽  
Phorbol Esters ◽  
Wnt Signaling Pathway ◽  
Fold Increase ◽  
Luciferase Assay ◽  
Bioassay Guided Fractionation ◽  
Wnt Signal

A screening of natural products using a luciferase assay targeting the Wnt signaling pathway was carried out, and the bioassay-guided fractionation of Excoecaria indica (Euphorbiaceae) collected from Bangladesh afforded three phorbol esters (1 – 3). These compounds exhibited Wnt signal-augmenting effects with 1 causing a 25-fold increase in TCF/β-catenin (TOP) transcriptional activity at 95 nM.

Left-right asymmetry in palatal rugae is associated with genetic variants in WNT signaling pathway

2020 ◽  
Vol 110 ◽  
pp. 104604 ◽  
Author(s):  
Alice Corrêa Silva-Sousa ◽  
Guido Artemio Marañón-Vásquez ◽  
Jennifer Tsi Gerber ◽  
Claudia S. Judachesci ◽  
Maria Bernadete Sasso Stuani ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Signaling Pathway ◽  
Genetic Variants ◽  
Wnt Signaling Pathway ◽  
Palatal Rugae ◽  
Left Right Asymmetry

scholarly journals TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction

2020 ◽  
Vol 8 ◽  
Author(s):  
Binbin Li ◽  
Lee A. Niswander
Keyword(s):  
Wnt Signaling ◽  
Signaling Pathway ◽  
Transmembrane Protein ◽  
Wnt Signaling Pathway ◽  
Regulatory Mechanisms ◽  
Fundamental Aspect ◽  
Adult Tissue ◽  
Functional Interaction ◽  
Ligand Interaction ◽  
Wnt Signal

Wnt signaling pathway plays indispensable roles in embryonic development and adult tissue homeostasis. However, the regulatory mechanisms involved in Wnt ligand trafficking within and secretion from the signal sending cells is still relatively uncharacterized. Here, we discover a novel regulator of Wnt signaling pathway called transmembrane protein 132A (TMEM132A). Our evidence shows a physical and functional interaction of TMEM132A with the Wnt ligand transporting protein Wntless (WLS). We show that TMEM132A stabilizes Wnt ligand, enhances WLS–Wnt ligand interaction, and activates the Wnt signaling pathway. Our results shed new light on the cellular mechanism underlying the fundamental aspect of WNT secretion from Wnt signal sending cells.

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