Analysis of Global Oxidative Status Using Multimarker Scores Reveals a Specific Association Between Renal Dysfunction and Diuretic Therapy in Older Adults

2021 ◽  
Author(s):  
Elena Rodríguez-Sánchez ◽  
José Alberto Navarro-García ◽  
Jennifer Aceves-Ripoll ◽  
Laura González-Lafuente ◽  
Nerea Corbacho-Alonso ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Older Adults ◽  
Renal Function ◽  
Oxidative Status ◽  
Protein Carbonyls ◽  
Multivariate Linear Regression Analysis ◽  
Older Population ◽  
Sod Activity ◽  
Pronounced Increase ◽  
Diuretic Treatment

Abstract Aging and chronic kidney disease (CKD) are important interrelated cardiovascular risk (CVR) factors linked to oxidative stress, but this relationship has not been well studied in older adults. We assessed the global oxidative status in an older population with normal to severely impaired renal function. We determined the oxidative status of 93 older adults (mean age 85 years) using multimarker scores. OxyScore was computed as index of systemic oxidative damage by analyzing carbonyl groups, oxidized low-density lipoprotein, 8-hydroxy-2′-deoxyguanosine, and xanthine oxidase activity. AntioxyScore was computed as index of antioxidant defense by analyzing catalase and superoxide dismutase (SOD) activity and total antioxidant capacity. OxyScore and AntioxyScore were higher in subjects with estimated glomerular filtration rate (eGFR) <60 mL/min/1.73 m2 than in peers with eGFR >60 mL/min/1.73 m2, with protein carbonyls, catalase, and SOD activity as major drivers. Older adults with a recent cardiovascular event had similar OxyScore and AntioxyScore as peers with eGFR >60 mL/min/1.73 m2. Multivariate linear regression analysis revealed that both indices were associated with decreased eGFR independently of traditional CVR factors. Interestingly, AntioxyScore was also associated with diuretic treatment, and a more pronounced increase was seen in subjects receiving combination therapy. The associations of AntioxyScore with diuretic treatment and eGFR were mutually independent. In conclusion, eGFR is the major contributor to the imbalance in oxidative stress in this older population. Given the association between oxidative stress, CKD, and CVR, the inclusion of renal function parameters in CVR estimators for older populations, such as the SCORE-OP, might improve their modest performance.

Oxidative stress as a mechanism of combined OTA and CTN toxicity in rat plasma, liver and kidney

2018 ◽  
Vol 38 (4) ◽  
pp. 434-445 ◽  
Author(s):  
D Rašić ◽  
V Micek ◽  
MS Klarić ◽  
M Peraica
Keyword(s):  
Oxidative Stress ◽  
Rat Plasma ◽  
Protein Carbonyl ◽  
Protein Carbonyls ◽  
Lower Plasma ◽  
Sod Activity ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Gpx Activity

Ochratoxin A (OTA) and citrinin (CTN) commonly coexist in grains. Aiming to evaluate oxidative stress in OTA + CTN toxicity, male Wistar rats were orally treated with two doses of OTA (0.125 and 0.250 mg kg−1 of body weight (b.w.)), CTN (2 mg kg−1 of b.w.) and resveratrol (RSV; 20 mg kg−1 of b.w.) and combined daily during 3 weeks. Protein carbonyl concentrations were measured in kidneys and liver; catalytic activity of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) level in plasma, kidneys and liver, while malondialdehyde (MDA) concentration was measured in plasma, kidneys, liver and urine. Mycotoxin treatment significantly increased MDA concentration in plasma and kidney and decreased SOD activity in the liver. Rats treated with CTN and OTA125 + CTN had lower plasma GPx activity. Concentration of GSH in the kidney and protein carbonyls in the kidney and liver as well as GPx activity in the kidney and liver, SOD activity in the kidney and CAT activity in the liver were not affected. Protective effect of RSV was observed on GSH in the kidney and plasma and MDA in the kidney, plasma and urine. Oxidative stress is involved in OTA + CTN toxicity in vivo because such treatment affects parameters of oxidative stress, particularly in plasma. RSV can reduce but not overcome oxidative stress induced by combined OTA and CTN treatment.

scholarly journals Oxidative stress in rat liver during acute cadmium and ethanol intoxication

2012 ◽  
Vol 77 (2) ◽  
pp. 159-176 ◽  
Author(s):  
Tatjana Radosavljevic ◽  
Dusan Mladenovic ◽  
Milica Ninkovic ◽  
Danijela Vucevic ◽  
Ivan Boricic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Rat Liver ◽  
Manganese Superoxide Dismutase ◽  
Treated Group ◽  
Sod Activity ◽  
Pronounced Increase ◽  
Orogastric Tube ◽  
Liver Copper ◽  
Male Wistar Rats

The aim of our study was to investigate the effects of binge drinking on prooxidant/antioxidant system in rat liver in acute cadmium (Cd) intoxication. In experiment male Wistar rats were used and divided into following groups: 1. control, 2. ethanol-treated group, in five subsequent doses of 2 g/kg administered by orogastric tube, 3. Cd-treated group in a single dose of 2.5 mg/kg intraperitoneally, 4. group that received Cd 12 hours after the last dose of ethanol. Blood and liver samples were collected for determination of oxidative stress parameters, 24 hours after treatment. When administered in combination, ethanol and Cd induced a more pronounced increase in serum and liver malondialdehyde level than either of these substances alone (p<0.01). Liver manganese superoxide dismutase (MnSOD) activity was increased both in ethanol and Cd-treated group (p<0.01), while liver copper/zinc superoxide dismutase (Cu/ZnSOD) activity was elevated in Cd group only. However, when administered in combination, ethanol and Cd induced a more pronounced decrease in liver MnSOD and Cu/ZnSOD activity 24 hours after treatment (p<0.01). Based on our study, it can be concluded that ethanol may act sinergistically with Cd in inducing lipid peroxidation and reduction in liver SOD activity.

scholarly journals Hydrogen Sulfide Inhibits High-Salt Diet-Induced Renal Oxidative Stress and Kidney Injury in Dahl Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Pan Huang ◽  
Zhizhou Shen ◽  
Jia Liu ◽  
Yaqian Huang ◽  
Siyao Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Antioxidative Activity ◽  
Kidney Injury ◽  
Renal Tissue ◽  
High Salt ◽  
Sod Activity ◽  
High Salt Diet ◽  
Salt Diet ◽  
Sd Rats

Background. The study was designed to investigate if H2S could inhibit high-salt diet-induced renal excessive oxidative stress and kidney injury in Dahl rats.Methods. Male salt-sensitive Dahl and SD rats were used. Blood pressure (BP), serum creatinine, urea, creatinine clearance rate, and 24-hour urine protein were measured. Renal ultra- and microstructures were observed. Collagen-I and -III contents the oxidants and antioxidants levels in renal tissue were detected. Keap1/Nrf2 association and Keap1 s-sulfhydration were detected.Results. After 8 weeks of high-salt diet, BP was significantly increased, renal function and structure were impaired, and collagen deposition was abundant in renal tissues with increased renal MPO activity, H2O2, MDA, GSSG, and•OH contents, reduced renal T-AOC and GSH contents, CAT, GSH-PX and SOD activity, and SOD expressions in Dahl rats. Furthermore, endogenous H2S in renal tissues was decreased in Dahl rats. H2S donor, however, decreased BP, improved renal function and structure, and inhibited collagen excessive deposition in kidney, in association with increased antioxidative activity and reduced oxidative stress in renal tissues. H2S activated Nrf2 by inducing Keap1 s-sulfhydration and subsequent Keap1/Nrf2 disassociation.Conclusions. H2S protected against high-salt diet-induced renal injury associated with enhanced antioxidant capacity and inhibited renal oxidative stress.

scholarly journals Effect of Tai Chi versus Walking on Oxidative Stress in Mexican Older Adults

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Juana Rosado-Pérez ◽  
Rocío Ortiz ◽  
Edelmiro Santiago-Osorio ◽  
Víctor Manuel Mendoza-Núñez
Keyword(s):  
Oxidative Stress ◽  
Older Adults ◽  
Tai Chi ◽  
Total Antioxidant Status ◽  
Antioxidant Effect ◽  
Control Group ◽  
Post Intervention ◽  
Sod Activity ◽  
Quasi Experimental ◽  
Walking Group

It has recently been reported that the practice of Tai Chi reduces oxidative stress (OxS), but it is not clear whether walking or Tai Chi produces a greater antioxidant effect. The aim of the present study was to evaluate the effect of the practice of Tai Chi and walking on markers for OxS. We carried out a quasi-experimental study with 106 older adults between 60 and 74 years of age who were clinically healthy and divided into the following groups: (i) control group (n=23), (ii) walking group (n=43), and (iii) Tai Chi group (n=31). We measured the levels of lipoperoxides (LPO), antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), and total antioxidant status (TAS) pre- and post-intervention in all subjects. The data were subjected to a covariant analysis. We found lower levels of LPO in the Tai Chi group compared with the walking group (Tai Chi, 0.261 ± 0.02; walking, 0.331 ± 0.02; control, 0.304 ± 0.023 µmol/L;P=0.05). Likewise, we observed significantly higher SOD activity and lower OxS-score in the Tai Chi group (P<0.05). Our findings suggest that the practice of Tai Chi produces a more effective antioxidant effect than walking.

scholarly journals Thyroid Function and Reduced Kidney Function with Aging in Chinese Older Adults: A Cross- sectional Study

2021 ◽  
Author(s):  
Lu Wei ◽  
Yun Bai ◽  
Yu Zhang ◽  
Zhenzhu Yong ◽  
Qun Zhang ◽  
...  
Keyword(s):  
Older Adults ◽  
Renal Function ◽  
Kidney Function ◽  
Thyroid Function ◽  
Older Individuals ◽  
Free Triiodothyronine ◽  
Older Population ◽  
Cross Sectional ◽  
Chinese Older Adults ◽  
Reduced Kidney Function

Abstract Background: Thyroid function may be a factor influencing renal function in general population. Renal function and thyroid function vary with aging, thus the association of thyroid function and declining kidney function in older adults was different from this in the young and remains controversial. The aim of this study was to estimate association between thyroid function in reference interval and reduced kidney function with aging in Chinese older adults.Methods: A total of 15729 adults, 23.3% (N= 3624) of which were older population, were collected in the health check-up department of the First Affiliated Hospital of Nanjing Medical University between January 2018 to January 2020.Basic demographic information was collected by physician-administered questionnaire. The variation tendency of thyroid function with aging was investigated by mean of free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulatinghormone (TSH) in subgroups for every 10 years. Associations between kidney and thyroid function were estimated with β value by multiple linear regression and with odds ratio (OR) by multivariable logistic regression models adjusted for age, gender, body mass index and serum urine acid. Results: In the older population, an increased trend of TSH, a decreased trend of FT3 and little change of FT4 were identified with aging. Estimated glomerular filtration rate (eGFR) remarkably decreased with increasing TSH (β: -0.081) and declining FT3 (β: 0.083) concentrations. Compared with the population in the lowest FT3 quartile (3.10-4.45 pmol/L), the prevalence of eGFR< 75ml/min/1.73m2 significantly decreased by 22.0% with FT3 4.46-4.81 pmol/L, 27.6% with FT3 4.82-5.20 pmol/L and 34.9% with FT3 5.21-6.8 pmol/L in older individuals (P for trend <.001). The OR was 1.315 with high-normal TSH, comparing to low-normal TSH in older persons (P: 0.025). Similar results were found between prevalent eGFR< 60ml/min/1.73m2 and thyroid function. The prevalent reduced kidney function was not remarkably associated with FT4 in the reference range.Conclusions: This study demonstrated a significant association between thyroid function and kidney function, especially FT3 in older population. The clinician were advised to evaluate renal function with low-normal FT3 and high-normal TSH in case of potential decreased kidney function.

scholarly journals Assessing the Optimum Level of Supplementation with Camelina Seeds in Ewes’ Diets to Improve Milk Quality

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2076
Author(s):  
Christos Christodoulou ◽  
Alexandros Mavrommatis ◽  
Christina Mitsiopoulou ◽  
George Symeon ◽  
Vasilis Dotas ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Milk Fat ◽  
Camelina Sativa ◽  
Oxidative Status ◽  
Milk Quality ◽  
Protein Carbonyls ◽  
Control Group ◽  
Optimum Level ◽  
Oxidative Stress Biomarkers ◽  
Sod Activity

Camelina sativa seeds are rich in bioactive compounds such as polyunsaturated fatty acids (PUFA) and antioxidants, thus, their supplementation in ewes’ diets, may be an effective way to develop high nutritional dairy products. Therefore, the present study investigates the effect of the dietary inclusion of Camelina sativa seeds in ewes’ oxidative status and milk quality. Forty-eight dairy Chios ewes were divided into four homogenous groups and were fed individually. The concentrate of the control group (CON) had no inclusion of Camelina seeds, while the treatment groups (CSS6, CSS11, CSS16) were supplemented with 6%, 11%, and 16%, respectively. Including Camelina seeds in 6% and 11%, had no impact on milk performance, while in the CSS16, milk fat was significantly decreased compared to the CON. Supplementing Camelina seeds improved milk quality from a human health perspective by modifying the content of saturated fatty acid, the proportions of α-linolenic (C18:3 n-3), and C18:2 cis-9, trans-11 (CLA), and the ω6/ω3 ratio. Furthermore, the activity of catalase (CAT) was significantly increased in the CSS11 and CSS16, and superoxide dismutase (SOD) activity also significantly upsurged in the CSS16. Still, the levels of malondialdehyde (MDA) were significantly increased in the CSS11 compared to the CON and CSS6, and in the CSS16 compared to the CSS6. In CSS16, protein carbonyls were significantly increased. Finally, in the CSS-fed ewes, milk oxidative stability was fortified, as suggested by the modifications in the activities of SOD, CAT, and glutathione peroxidase (GSH-Px), in the antioxidant capacity, and the oxidative stress biomarkers. Consequently, the incorporation of 6% Camelina seeds in the concentrates of ewes improves milk’s fatty acid profile and oxidative status. However, more research is required regarding the possible negative effects of the constant consumption of Camelina seeds by ewes.

scholarly journals Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

2020 ◽  
Vol 70 (3) ◽  
pp. 355-373
Author(s):  
Biljana Zikic ◽  
Nevenka Aleksic ◽  
Marko Ristanic ◽  
Uros Glavinic ◽  
Branislav Vejnovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Honey Bees ◽  
Oxidative Status ◽  
Food Storage ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Malonyl Dialdehyde ◽  
Per Se ◽  
Bee Colonies

AbstractApart from the efficiency of coumaphos against Varroa mites, its impact on the oxidative status and survival of the honey bee (Apis mellifera) was assessed. The research was conducted on hives from the same apiary, equalised regarding the number of bees, brood area and food storage. Based on Varroa infestation the hives were allotted to two groups: non-infested (N) and infested (I). Both groups were either treated (T) – NT and IT, or untreated (U) – NU and IU. The treatment of infested bees was controlled with a follow-up treatment with amitraz. The efficiency of coumaphos was 96-97%. This organophosphate had a negligible effect on bee survival, but it significantly affected their oxidative status: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, and the concentrations of malonyl dialdehyde (MDA). Coumaphos significantly (p˂0.0001) decreased SOD activity in non-infested bees, but increased it in those infested. By contrast, both CAT and GST activities, as well as MDA concentrations significantly increased (from p˂0.05 to p˂0.0001) after treatment in all groups, with the exception of IT, where it declined. Coumaphos in non-infested hives caused oxidative stress per se, not unlike varroa in infested colonies. However, in infested colonies it decreased oxidative stress, owing to its efficacy against Varroa mites and contributed to the recovery of bee colonies. In spite of its certain downsides, coumaphos remains an effective anti-varroa substance, but should be used with precaution, not to add to the effects of environmental factors which may cause red-ox misbalance.

scholarly journals ERYTHROCYTE OXIDATIVE STATUS AFTER MAXIMAL AEROBIC TEST IN WRESTLERS

2019 ◽  
Vol 19 (1) ◽  
pp. 15-21
Author(s):  
A Alexandrova ◽  
L Petrov ◽  
R Makaveev ◽  
E Tsvetanova ◽  
A Georgieva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Venous Blood ◽  
Hemoglobin Concentration ◽  
Oxidative Status ◽  
Total Glutathione ◽  
Sod Activity ◽  
Blood Samples

Aim. The aim of this study was to determine the changes in the erythrocyte oxidative status of the wrestlers after performing the maximal aerobic test, by registering in erythrocytes the levels of lipid peroxidation (LPO), total glutathione (tGSH) and activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). Materials and methods. A group of 12 healthy wrestlers conducted a treadmill maximal aerobic test, and venous blood samples were obtained before and immediately after the exercise. Erythrocytes were separated from plasma and used for spectrophotometric determination of LPO, tGSH and enzyme activities. Plasma was used for determination of hemoglobin concentration (Hb) as an index of hemolysis. Results. The performance of the maximal aerobic test resulted in a significant increase of Hb in blood plasma, a decrease of LPO, and no changes of the tGSH level in erythrocytes. In regards to antioxidant enzymes, our results showed an increase in the activity of GPx, while the CAT and SOD activity remain unchanged. Conclusions. It can be concluded that in active athletes, predominate erythrocytes that are more resistant to oxidative stress, because of the accelerated hemolysis induced by physical exercise, lead to the elimination of the old and oxidative modified cells.

scholarly journals Oxidative protein damage is associated with poor grip strength among older women living in the community

2007 ◽  
Vol 103 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Caitlin Howard ◽  
Luigi Ferrucci ◽  
Kai Sun ◽  
Linda P. Fried ◽  
Jeremy Walston ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Older Adults ◽  
Muscle Strength ◽  
Grip Strength ◽  
Older Women ◽  
Protein Carbonyl ◽  
Protein Damage ◽  
Protein Carbonyls ◽  
Cross Sectional ◽  
Oxidative Protein Damage

Grip strength, an indicator of muscle strength, has been shown to be a predictor of poor outcomes among older adults. Protein carbonylation, an indicator of oxidative damage to proteins, leads to cellular dysfunction and a decline in tissue function. Oxidative stress has been implicated in the pathogenesis of sarcopenia. The objective was to determine whether serum protein carbonyl concentrations are associated with grip strength in older women living in the community. A cross-sectional study was conducted in 672 women, aged 65 and older, from the Women's Health and Aging Study (WHAS) I, the one-third most disabled women residing in the community in Baltimore, MD. Protein carbonyl and grip strength were measured in each patient. In a multivariate analysis adjusting for age, race, body mass index, and Mini-Mental Status Examination score, protein carbonyls (nmol/mg) were associated with grip strength (β = −6.77, P < 0.01). The statistical association was unchanged after the analysis adjusted for hypertension, congestive heart failure, and depression. Ordered logistic regression models adjusted for the above factors showed that protein carbonyls are associated with increased odds of being in the lower quartiles of grip strength (odds ratio 8.74, 95% confidence interval 1.06–71.89, P = 0.043). These results suggest oxidative protein damage is independently associated with low grip strength among older women living in the community. Increased oxidative stress may be contributing to loss of muscle strength in older adults.

Association Between Multimorbidity and Quality of Life Among Older Adults in Community-Dwelling of Uttar Pradesh, India

2021 ◽  
Author(s):  
Himanshu Himanshu ◽  
P. Arokiasamy ◽  
Y. Selvamani
Keyword(s):  
Quality Of Life ◽  
Risk Factors ◽  
Older Adults ◽  
Communicable Disease ◽  
Linear Regression Analysis ◽  
Functional Limitation ◽  
Community Dwelling ◽  
Multivariate Linear Regression Analysis ◽  
Older Population

Abstract Background The rise in life expectancy and the share of older population represents the most significant demographic transformation in the twenty-first century. Increasing longevity with the coexistence of chronic multimorbidity makes the elderly population vulnerable to functional limitation, disability and more frequent hospitalization, resulting deterioration in QoL. The present study aims to investigate the association between non-communicable disease (NCD) multimorbidity and QoL among the older population in Varanasi, India. Methods A cross-sectional data of 500 individuals in the Varanasi district aged 50 + years were collected, using a multistage simple random sampling procedure from November 2017 to May 2018. WHOQOL-BREF was utilised to assess the quality of life of the study participants, and the important covariates used in the analysis are; age, sex, marital status, place of residence, health factors, socioeconomic status, and behavioral risk factors. Descriptive analysis was performed to assess the mean QoL score pattern, whereas multivariate linear regression analysis examines the association between multimorbidity and QoL. Results The QoL scores decreased with age and was higher among females. Regression results show that demographic and lifestyle risk factors are closely associated with QoL. multimorbidity was significantly associated with reduced quality of life. Older adults with multimorbidity had 5 points lower quality of life than those with no chronic diseases. Conclusions Multimorbidity along with demographic and lifestyle factors are significantly associated with QoL. Healthcare programmes need to factor in multimorbidity while promoting a healthy and risk-free lifestyle to control modifiable risk factors. Government assistance is necessary for the most economically dependent older population for their day-to-day needs.

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