scholarly journals Parallux™ β-Lactam: A Capillary-Based Fluorescent Immunoassay for the Determination of Penicillin-G, Ampicillin, Amoxicillin, Cloxacillin, Cephapirin, and Ceftiofur in Bovine Milk

2002 ◽  
Vol 85 (2) ◽  
pp. 355-364 ◽  
Author(s):  
Stacey P Huth ◽  
Polly S Warholic ◽  
Jane M Devou ◽  
Larry K Chaney ◽  
Genevieve H Clark
Keyword(s):  
Bovine Milk ◽  
Penicillin G ◽  
Antibiotic Residues ◽  
Test Results ◽  
Glass Capillary ◽  
Glass Capillaries ◽  
Minimum Sensitivity ◽  
Mastitic Milk ◽  
Analytical System

Abstract An analytical system was developed for detection of antibiotic residues in bovine milk. The method is based on competitive fluorescent immunoassays in glass capillary tubes (U.S. Patent No. 5,624,850). The system consists of an assay cartridge containing 4 glass capillaries, a reagent tray with 4 wells of dried reagents, and a Parallux™ processor, which processes the assay, reads fluorescent output, and reports test results. Minimum sensitivity for detection of 6 β-lactam antibiotics in bovine milk was determined to be penicillin-G, 3.2 ppb; ampicillin, 2.9 ppb; amoxicillin, 3.6 ppb; cloxacillin, 7.4 ppb; cephapirin, 16.3 ppb; and ceftiofur, 33.7 ppb. The assay system was also specific and sensitive for detection of incurred residues at U.S. Food and Drug Administration tolerance levels: penicillin-G, 5 ppb; ampicillin, 10 ppb; amoxicillin, 10 ppb; cloxacillin, 10 ppb; cephapirin, 20 ppb; and ceftiofur, 50 ppb. There was no interference in detection of minimum sensitivity levels of antibiotic by the presence of somatic cells at approximately 1 × 106 cells/mL. Milk containing 3 × 106 cells/mL bacteria commonly found in mastitic milk also showed no interference when tolerance levels of antibiotic were present. There was no detectable interference on results by a wide variety of non-β-lactam drugs.

Simultaneous Multiresidue Analysis of (β-Lactam Antibiotics in Bovine Milk by Liquid Chromatography with Ultraviolet Detection and Confirmation by Electrospray Mass Spectrometry

1994 ◽  
Vol 77 (5) ◽  
pp. 1122-1131 ◽  
Author(s):  
Krystyna L Tyczkowska ◽  
Robert D Voyksner ◽  
Rolf F Straub ◽  
Arthur L Aronson
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Electrospray Mass Spectrometry ◽  
Limit Of Detection ◽  
Water Solution ◽  
Bovine Milk ◽  
Penicillin G ◽  
Array Detector

Abstract A multiresidue analytical method was developed for the simultaneous determination of amoxicillin, cephapirin, procaine penicillin G, ampicil-lin, cloxacillin, and ceftiofur in bovine milk. The method involved ultrafiltration of milk diluted with an equal volume of 50% acetonitrile through a 10 000 dalton molecular mass cutoff filter. Separation of these β-lactam antibiotics from other milk components was performed by ion-paired (octane- and dodecanesulfonate) liquid chromatography using a phenyl column eluted with acetonitrile-water solution. Ultraviolet ab-sorbance of the column effluent was monitored in the 200-350 nm range of a photodiode-array detector. For quantitation, the chromatograms were acquired at λ210 nm for penicillin G, am-picillin, and cloxacillin; λ230 nm for amoxicillin; and λ290 for cephapirin, procaine, and ceftiofur. The limit of detection for the simultaneous determination of these antibiotics was estimated to be 100 ppb. Liquid chromatography/electrospray mass spectrometry could be used to confirm these antibiotics for quantities down to 100 pg entering the mass spectrometer.

Validation of Antibiotic Residue Tests for Dairy Goats

1998 ◽  
Vol 61 (3) ◽  
pp. 344-349 ◽  
Author(s):  
S. S. ZENG ◽  
S. HART ◽  
E. N. ESCOBAR ◽  
K. TESFAI
Keyword(s):  
High Performance ◽  
Goat Milk ◽  
High Sensitivity ◽  
Penicillin G ◽  
Antibiotic Residues ◽  
Test Results ◽  
Beta Lactam ◽  
Dairy Goats ◽  
Test Kit ◽  
Antibiotic Residue

The SNAP test, LacTek test (B-L and CEF), Charm Bacillus sterothermophilus var. calidolactis disk assay (BsDA), and Charm II Tablet Beta-lactam sequential test were validated using antibiotic-fortified and -incurred goat milk following the protocol for test kit validations of the U.S. Food and Drug Administration Center for Veterinary Medicine. SNAP, Charm BsDA, and Charm II Tablet Sequential tests were sensitive and reliable in detecting antibiotic residues in goat milk. All three assays showed greater than 90% sensitivity and specificity at tolerance and detection levels. However, caution should be taken in interpreting test results at detection levels. Because of the high sensitivity of these three tests, false-violative results could be obtained in goat milk containing antibiotic residues below the tolerance level. Goat milk testing positive by these tests must be confirmed using a more sophisticated methodology, such as high-performance liquid chromatography, before the milk is condemned. LacTek B-L test did not detect several antibiotics, including penicillin G, in goat milk at tolerance levels. However, LacTek CEF was excellent in detecting ceftiofur residue in goat milk.

A Rapid SPE-Based Analytical Method for UPLC/MS/MS Determination of Aminoglycoside Antibiotic Residues in Bovine Milk, Muscle, and Kidney

2014 ◽  
Vol 97 (6) ◽  
pp. 1737-1741 ◽  
Author(s):  
Michael S Young ◽  
Kim van Tran ◽  
Evelyn Goh ◽  
Jeremy C Shia
Keyword(s):  
High Throughput ◽  
Analytical Method ◽  
Bovine Milk ◽  
Aminoglycoside Antibiotic ◽  
Aminoglycoside Antibiotics ◽  
Antibiotic Residues ◽  
Precision Data ◽  
The Common ◽  
Interday Precision

Abstract An SPE-based cleanup protocol was developed for ultra-performance LC (UPLC)/MS/MS determination of residues of the common aminoglycoside antibiotics streptomycin, dihydrostreptomycin, neomycin, and gentamicin in bovine milk, kidney, and muscle. Recoveries for all compounds except neomycin ranged from 80 to 104% for all matrixes studied; recoveries for neomycin ranged from 71 to 84%. Intraday and interday precision data were under 15% for all sample matrixes. Compared with other recently reported cleanup methods, less sample is required, the use of potentially dangerous reagents is minimized, and fewer manipulations are required by the analyst. A high throughput 96-well plate format was used for SPE cleanup and UPLC/MS analysis.

scholarly journals Determination of Listeria monocytogenes and Serotypes in Modified Atmosphere Packed Ground and Cubed Beef Samples

2018 ◽  
Vol 6 (3) ◽  
pp. 365
Author(s):  
Adem Özkiraz ◽  
Ali Gücükoğlu
Keyword(s):  
Monitoring Program ◽  
Antibiotic Use ◽  
The Other ◽  
Penicillin G ◽  
Modified Atmosphere ◽  
Test Results ◽  
Pcr Method ◽  
Resistant Strains ◽  
Hlya Gene

This study was conducted to determine the Listeria monocytogenes’s presence, serotypes and resistance against various antibiotics in modified atmosphere packaged (MAP) ground and cubed beef samples. Five of ground (5/50-10%) and 3 of cubed beef samples (3/50-6%) were identified as L. monocytogenes positive in MAP samples. Eleven L. monocytogenes isolates that obtained from samples being investigated in term of hlyA gene by PCR method have verified that this gene (100%). In serotyping results, 3 of 8 isolate that obtained from MAP ground beef samples were 1/2a, the other 3 isolate was 1/2b and the remaining 2 isolate was 4b. Also, 1 of 3 isolate that obtained from MAP cubed beef samples were 1/2b, the other one isolate was 1/2c and the last one was 4b. One isolate against (9%) ampicillin, 2 isolate against (18.2%) chloramphenicol, 3 isolate against (27.2%) erythromycin, 4 isolate against (36.3%) oxytetracycline and 4 isolate against (36.3%) penicillin G, 6 isolate against (54.5%) tetracycline and 3 isolate against (27.2%) vancomycin was resistant in 11 L. monocytogenes isolates that confirmed by PCR. The L. monocytogenes isolates were found to be resistant to one or more antibiotics in antibiotic-resistance test results. In conclusion, applying of national residue monitoring program by official authority for prevention of intensive antibiotic use in order to prevent the development of resistant strains to antibiotics has great importance.

Sign in / Sign up

Export Citation Format

Share Document