scholarly journals Extraction of Aucubin from Seeds of Eucommia ulmoides Oliv. Using Supercritical Carbon Dioxide

2009 ◽  
Vol 92 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Hui Li ◽  
Jiangyu Hu ◽  
Hui Ouyang ◽  
Yanan Li ◽  
Hui Shi ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
High Yield ◽  
Eucommia Ulmoides ◽  
Extraction Temperature ◽  
Supercritical Co2 Extraction ◽  
Extraction And Separation ◽  
Soxhlet Method ◽  
Co2 Flow

Abstract Supercritical CO2 was used as solvent for the extraction of aucubin from the seeds of Eucommia ulmoides Oliv. The co-solvent composition was tested and extraction conditions were optimized. Results showed that the best co-solvent was a waterethanol mixture (1 + 3, v/v), and the highest yield was obtained when the extraction was performed under 26 MPa at extraction and separation temperatures of 55 and 30C for 120 min, using 6 mL co-solvent/g material at a CO2 flow rate of 20 L/h. In a comparison of the supercritical CO2 and Soxhlet extraction methods, the Soxhlet method needed 3 h to extract 10 g material, whereas the supercritical CO2 extraction technique needed only 2 h to extract 100 g material, thus showing a high extraction capability. The supercritical CO2 extraction produced a higher yield, with a lower cost for the extraction. Owing to the advantages of low extraction temperature, high yield, and ease of separating the product from the solvent, supercritical CO2 extraction is likely to be developed into an ideal technique for the extraction of aucubin, a compound with thermal instability, from the seeds of this plant.

scholarly journals Optimized Supercritical CO2 Extraction Enhances the Recovery of Valuable Lipophilic Antioxidants and Other Constituents from Dual-Purpose Hop (Humulus lupulus L.) Variety Ella

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 918
Author(s):  
Nóra Emilia Nagybákay ◽  
Michail Syrpas ◽  
Vaiva Vilimaitė ◽  
Laura Tamkutė ◽  
Audrius Pukalskas ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Antioxidant Properties ◽  
Extraction Yield ◽  
Biologically Active ◽  
High Yield ◽  
Acid Extraction ◽  
Supercritical Co2 Extraction ◽  
Bioactive Constituents ◽  
Total Extract

The article presents the optimization of supercritical CO2 extraction (SFE-CO2) parameters using response surface methodology (RSM) with central composite design (CCD) in order to produce single variety hop (cv. Ella) extracts with high yield and strong in vitro antioxidant properties. Optimized SFE-CO2 (37 MPa, 43 °C, 80 min) yielded 26.3 g/100 g pellets of lipophilic fraction. This extract was rich in biologically active α- and β-bitter acids (522.8 and 345.0 mg/g extract, respectively), and exerted 1481 mg TE/g extract in vitro oxygen radical absorbance capacity (ORAC). Up to ~3-fold higher extraction yield, antioxidant recovery (389.8 mg TE/g pellets) and exhaustive bitter acid extraction (228.4 mg/g pellets) were achieved under the significantly shorter time compared to the commercially used one-stage SFE-CO2 at 10–15 MPa and 40 °C. Total carotenoid and chlorophyll content was negligible, amounting to <0.04% of the total extract mass. Fruity, herbal, spicy and woody odor of extracts could be attributed to the major identified volatiles, namely β-pinene, β-myrcene, β-humulene, α-humulene, α-selinene and methyl-4-decenoate. Rich in valuable bioactive constituents and flavor compounds, cv. Ella hop SFE-CO2 extracts could find multipurpose applications in food, pharmaceutical, nutraceutical and cosmetics industries.

scholarly journals PENGARUH METODE EKSTRAKSI MINYAK PUPA ULAT JERMAN TERHADAP KOMPOSISI KIMIA

BUANA SAINS ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 67
Author(s):  
Erik Priyo Santoso ◽  
Farida Kusuma Astuti
Keyword(s):  
Extraction Method ◽  
Crude Protein ◽  
Dry Matter ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Chemistry Laboratory ◽  
Research Material ◽  
Soxhlet Method ◽  
Heat Method

The aim of this research is to know the extraction method of caterpillar pupa without detrimental to chemical composition to know the amount of pupa production of German caterpillar with two extraction methods per 100 grams of pupa To know the content of dry matter, organic matter, crude protein, crude fat, crude fiber and amino acid lysin, methionine, and vitamin E pupa oil of German caterpillar. The study was conducted from February to April 2017, at the Lab. Central University Tribhuwana Tunggadewi. Oil extraction analysis was conducted in the chemistry laboratory of Tribhuwana Tunggadewi University and chemistry laboratory of Poltek Malang. German caterpillar research material, German caterpillar pupa, pupa caterpillar germany extracted by maceration and sokletasi. German caterpillar pupa is obtained from German caterpillar breeders in Amadanom, Dampit, Malang Regency. This research uses two different extraction methods, namely cold extraction method (maseration method) and using heat method (soxhlet method). The conclusions of both research methods are known that the quantity of German caterpillar yield using soxhlet extraction method is more than the maceration extraction method. However, for the quality of the two methods it is still necessary to do further analysis to know the exact comparison of the two methods.

scholarly journals Preparation of Ganglioside GM1 by Supercritical CO2 Extraction and Immobilized Sialidase

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3732
Author(s):  
Li Ji ◽  
Zhonghui Qiao ◽  
Xin Zhang ◽  
Xiaolei Cheng ◽  
Weiyang Wang ◽  
...  
Keyword(s):  
Neurological Disorders ◽  
Supercritical Co2 ◽  
Brain Diseases ◽  
High Yield ◽  
Acetone Powder ◽  
Supercritical Co2 Extraction ◽  
Ganglioside Gm1 ◽  
Pig Brain ◽  
Optimum Extraction ◽  
Brain Gangliosides

Monosialotetrahexosylganglioside (GM1) has good activity on brain diseases and was developed to be a drug applied in clinics for neurological disorders and nerve injury. It is difficult to isolate GM1 in industry scale from the brains directly. In this work, a simple and highly efficient method with high yield was developed for the isolation, conversion, and purification of GM1 from a pig brain. Gangliosides (GLS) were first extracted by supercritical CO2 (SCE). The optimum extraction time of GLS by SCE was 4 h, and the ratio of entrainer to acetone powder from the pig brain was 3:1 (v/w). GM1 was then prepared from GLS by immobilized sialidase and purified by reverse-phase silica gel. Sodium alginate embedding was used for the immobilization of sialidase. Under the optimized method, the yield of high-purity GM1 was around 0.056%. This method has the potential to be applied in the production of GM1 in the industry.

scholarly journals Ultrasonic Extraction of Oil fromCaesalpinia spinosa(Tara) Seeds

2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
Zhan-jun Li ◽  
Feng-jian Yang ◽  
Lei Yang ◽  
Yuan-Gang Zu
Keyword(s):  
Fatty Acid ◽  
Seed Oil ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Ultrasonic Extraction ◽  
Particle Sizes ◽  
Extraction Temperature ◽  
Simple Method ◽  
Alternative Means ◽  
Fatty Acid Compositions

Oil extracted from the seeds ofCaesalpinia spinosa(common name: tara) can be used in a number of applications. In the present study, tara seed oil was obtained by ultrasonic extraction. The effects of different solvents, particle sizes in the ground seed samples, extraction times, ultrasonication powers, extraction temperatures, and liquid–solid ratios on the yield of tara seed oil were investigated. The yield from the ultrasonic extraction was compared with that from traditional Soxhlet extraction. The results showed that ultrasonic extraction could be completed in a shorter time with reduced solvent consumption. The yield of tara seed oil increased with increasing ultrasonication power and extraction temperature. Gas chromatography was used to analyze the fatty acid compositions of the oils extracted by the two methods. The fatty acid compositions of the oils from both extraction methods were similar, which indicates that ultrasonic extraction is a viable alternative means of extraction. It is a rapid, efficient, and simple method for production of lipids from tara seeds.

scholarly journals Obtaining of β-Sitosterol from Cardamom by Supercritical CO2 Extraction

2017 ◽  
Author(s):  
Hacer İçen ◽  
Hatice Tuğba Çelik ◽  
Mustafa Serhat Ekinci ◽  
Metin Gürü
Keyword(s):  
Flow Rate ◽  
Supercritical Co2 ◽  
Oil Yield ◽  
Gas Chromatography Mass Spectrometry ◽  
Operating Parameters ◽  
Protective Effects ◽  
Supercritical Co2 Extraction ◽  
Cosmetic Industry ◽  
Co2 Flow ◽  
Optimized Conditions

Cardamom is plant of the Zingiberaceae family. It has been used for the treatment of many diseases such as migraine, bronchitis, stomach and intestinal disorders. Cardamom contains triterpenes, resins, starch and fatty compounds. Phytosterols (stigmasterol, campesterol and &beta;-sitosterol) are a group steroid alcohol in plants. They are used food, medicine and cosmetic industry. They are protective effects against some types of cancer too. Phytosterols are found in the vegetable oil such as the spindle, corn and soybean oil. This paper deals with the maximum oil and &beta;-sitosterol yield were investigated by means of the supercritical CO2 extraction of cardamom. The effect of operating parameters as temperature, pressure and CO2 flow rate were investigated on oil yield. The amount of &beta;-sitosterol was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) at optimized conditions. The optimized conditions were recorded as temperature of 40oC, pressure of 200 bar and CO2 flow rate of 4 L/min. The maximum oil yield and amount of &beta;-sitosterol were found as 74.83 mg oil/g seed and 4.73 mg &beta;-sitosterol/g seed cardamom under these conditions.

scholarly journals Comparative Analysis of Far East Sikhotinsky Rhododendron (Rh. sichotense) and East Siberian Rhododendron (Rh. adamsii) Using Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3774
Author(s):  
Mayya Razgonova ◽  
Alexander Zakharenko ◽  
Sezai Ercisli ◽  
Vasily Grudev ◽  
Kirill Golokhvast
Keyword(s):  
Far East ◽  
Selective Extraction ◽  
Extraction Methods ◽  
Biologically Active ◽  
Supercritical Co2 Extraction ◽  
Active Components ◽  
Esi Ms ◽  
Naturally Occurring ◽  
Target Analytes ◽  
Co2 Flow

Rhododendron sichotense Pojark. and Rhododendron adamsii Rheder have been actively used in ethnomedicine in Mongolia, China and Buryatia (Russia) for centuries, as an antioxidant, immunomodulating, anti-inflammatory, vitality-restoring agent. These plants contain various phenolic compounds and fatty acids with valuable biological activity. Among green and selective extraction methods, supercritical carbon dioxide (SC-CO2) extraction has been shown to be the method of choice for the recovery of these naturally occurring compounds. Operative parameters and working conditions have been optimized by experimenting with different pressures (300–400 bar), temperatures (50–60 °C) and CO2 flow rates (50 mL/min) with 1% ethanol as co-solvent. The extraction time varied from 60 to 70 min. A HPLC-UV-VIS-ESI-MS/MS technique was applied to detect target analytes. A total of 48 different biologically active components have been identified in the Rh. adamsii SC-CO2 extracts. A total of 31 different biologically active components have been identified in the Rh. sichotense SC-CO2 extracts.

scholarly journals Comparison of extraction methods for the hypotensive drugs from Eucommia ulmoides

2010 ◽  
Vol 62 (3) ◽  
pp. 725-730
Author(s):  
Hui Cao
Keyword(s):  
Organic Solvent ◽  
Supercritical Fluid ◽  
Human Body ◽  
Large Scale ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Eucommia Ulmoides ◽  
Plant Materials ◽  
Ph Values ◽  
Efficient Extraction

Extraction methods using Soxhlet extraction (SE), enzyme-assisted aqueous extraction (EE), semi-bionic extraction (SBE) and supercritical fluid extraction (SFE) were evaluated for the yields of geniposidic acid (GPA) and geniposide (GPS) from the bark of Eucommia ulmoides. The results showed that the yields of GPA and GPS attained by EE and SBE were highest, respectively. Compared with various extraction methods for the extraction of GPA and GPS, the SBE was more efficient than other methods. There was no organic solvent used in SBE. The pH values of semi-bionic extraction were the same as in the human body. The results indicate that SBE can be used for large-scale and efficient extraction of GPA and GPS from plant materials. The time taken by SFE was longer than other methods, but the yields of GPA and GPS were the lowest. .

scholarly journals Extraction, Modelling and Purification of Flavonoids from Citrus Medica Peel

2015 ◽  
Vol 3 (4) ◽  
pp. 588-591 ◽  
Author(s):  
M. Parvathi Nandan ◽  
Vangalapati Meena
Keyword(s):  
Solvent Extraction ◽  
Column Chromatography ◽  
Chemical Constituents ◽  
Soxhlet Extraction ◽  
High Yield ◽  
Phytochemical Analysis ◽  
Extraction And Separation ◽  
Proposed Model ◽  
Citrus Medica ◽  
Solvent Solvent

Soxhlet extraction technique is widely employed for the extraction and separation of chemical constituents in the medicinal plants. Citrus medica L commonly called as Citron belongs to family Rutaceae, is a slow-growing shrub. It is mainly cultivated for the production of edible fruits which are sour in taste like lime and lemon and the main content of a citron fruit is the thick rind, which is very adherent to the segments. From the phytochemical analysis the peel extract is rich source of phenols, flavonoids and alkaloids. The objective of present work is to develop a modelling equation for quercetin, rutin and kaempferol and the crude extract obtained by soxhlet extraction was further purified by solvent-solvent extraction and Column chromatography. Extraction was carried out by 80% methanol as a solvent Soxhlet extractor. Soxhlet extraction with methanol was carried out with varying time intervals, to evaluate modelling equation. The proposed modelling equation was Es = 0.0849(t) + 7.0286 for Quercetin and Es = 0.0912(t) + 25.971 for rutin, and Es = 0.0267(t) + 7.3714 for Kaempferol. High yield was obtained for 180min of Soxhlet extraction with 80% methanol. Yield of quercetin, rutin and kaempferol after solvent-solvent extraction and column chromatography was 22.6µg/ml, 43.7µg/ml and 10.8µg/ml respectively. The proposed model showed good agreement with the experimental data. Int J Appl Sci Biotechnol, Vol 3(4): 588-591

Sign in / Sign up

Export Citation Format

Share Document