278 Feeding a whole-cell inactivated Pichia guilliermondi yeast to gestating and lactating sows over two consecutive parities

Abstract A total of 535 sows and their progeny (PIC 359×1050) were used to determine the effect of feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product (WCY; CitriStim, ADM Animal Nutrition, Quincy, IL) on sow and litter performance in a commercial production system. Within 24 hours of breeding, sows were allotted to 1 of 2 dietary treatments. Parity distribution was equalized across treatments. Sows were fed either a basal gestation control (CON) diet or CON fortified with 0.1% (0.91 kg/ton) of the WCY at the expense of corn throughout gestation. Sow dietary treatments were also maintained through lactation. Of the 535 sows that completed the initial reproductive cycle (cycle 1), 358 sows were maintained on dietary treatment and followed through a second gestation and lactation (cycle 2). The direction and magnitude of the differences in total born and born alive pigs in cycle 1 and cycle 2 was similar between treatments. Supplementing sows with WCY in cycle 1 increased the number of total born pigs by 0.59 pigs/litter (P=0.038) and tended to increase the number of pigs born alive by 0.52 pigs/litter (P=0.064) compared to CON fed sows. For cycle 2, the total number of born and born alive pigs numerically increased by 0.44 pigs/litter (P=0.180) and 0.43 pigs/litter (P=0.179) respectively for sows fed WCY. The distribution of pig birth weights was not different between treatments in cycle 1 (P=0.536) nor cycle 2 (P=0.256). Also, the litter growth rate on a weight gain per pig per day basis was not different between treatments in cycle 1 (P=0.103) nor cycle 2 (P=0.379). In conclusion, feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product significantly increased the number of total born pigs during cycle 1.

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275 Feeding a whole-cell inactivated Pichia guilliermondi yeast to gestating and lactating sows in a commercial production system

Journal of Animal Science ◽

10.1093/jas/skaa054.172 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 99-100

Author(s):

Morgan T Thayer ◽

Ricardo M Garcia ◽

Alan W Duttlinger ◽

Julie F Mahoney ◽

Allan P Schinckel ◽

...

Keyword(s):

Body Weight ◽

Litter Size ◽

Production System ◽

Control Diet ◽

Commercial Production ◽

Animal Nutrition ◽

Whole Cell ◽

Lactating Sows ◽

Yeast Product ◽

Dietary Treatments

Abstract A total of 606 sows (PIC 1050) and their progeny (PIC 1050×280) were used to determine if feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product (WCY; CitriStim, ADM Animal Nutrition, Quincy, IL) improves sow and litter performance in a commercial production system. Once confirmed pregnant at d 35 post-breeding pregnancy check, sows were fed either a basal gestation control (CON) diet (0.55% SID Lysine) or the control diet fortified with 0.15% (1.36 kg/ton) of the WCY replacing corn in the CON diet. Dietary treatments were also fed in lactation (1.05% SID Lysine) once sows were moved into farrowing crates on approximately d 112 of gestation until weaning. Sows supplemented with WCY in gestation and lactation had a greater number of total born piglets by 0.45 pigs (P=0.040), born alive (P< 0.04), heavier born alive litter weight (P< 0.001), and greater post cross-foster litter size (P< 0.001) compared to CON fed sows. Litter size at weaning was increased by 0.54 pigs when sows were fed WCY compared to CON (P< 0.001). However, the average piglet weaning weights from CON fed sows were heavier by 0.35 kg compared to WCY (P< 0.001). This increase in body weight of piglets from CON fed sows is partially explained by their 0.93 days longer lactation (P< 0.001), and may also be due to the smaller litter size nursed throughout lactation. The percent of litters treated for scours decreased from 38.3% to 14.2% when sows were fed WCY (P< 0.001). The distribution of birth weights by body weight range was not different (P=0.2461) between treatments. In conclusion, feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product increased the number of piglets born and number weaned as well as decreased the prevalence of scours during lactation.

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Comparative digestibility of polysaccharide-complexed zinc and zinc sulfate in diets for gestating and lactating sows

Journal of Animal Science ◽

10.1093/jas/skaa079 ◽

2020 ◽

Vol 98 (4) ◽

Author(s):

Julia P Holen ◽

Lee J Johnston ◽

Pedro E Urriola ◽

Jack E Garrett ◽

Gerald C Shurson

Keyword(s):

Soybean Meal ◽

Zinc Sulfate ◽

Fixed Effects ◽

Nutrient Balance ◽

Dietary Treatment ◽

High Fiber ◽

Polysaccharide Complex ◽

Distillers Grains With Solubles ◽

Lactating Sows ◽

Dietary Treatments

Abstract We hypothesized that the digestibility of a zinc polysaccharide complex is greater than zinc sulfate when sows consume high fiber diets containing corn dried distillers grains with solubles (DDGS). Gilts and sows (n = 32) were blocked according to parity and assigned randomly to one of four dietary treatments (n = 8 sows per treatments). Dietary treatments consisted of: 1) Control (ConZnSO4)—corn–soybean meal-based diet + 100 ppm supplemental Zn from ZnSO4; 2) Control PSZn (ConPSZn)—corn–soybean meal-based diet + 100 ppm supplemental Zn from Zn polysaccharide complex; 3) DDGS/ZnSO4—corn–soybean meal–40% DDGS gestation diet and a 30% DDGS lactation diet, with each containing 100 ppm supplemental Zn from ZnSO4; 4) DDGS/PSZn—corn–soybean meal–40% DDGS gestation diet and a 30% DDGS lactation diet, with each containing 100 ppm supplemental Zn from Zn polysaccharide complex. A fifth dietary treatment was imposed using a subset of sows (n = 20) to determine basal Zn losses in gestating and lactating sows fed corn–soybean meal-based diets containing no supplemental Zn. Nutrient balance experiments were conducted in both gestation and lactation to evaluate the digestibility of Zn sources of the four dietary treatments and to determine basal Zn losses when no supplemental Zn was provided. The statistical model included fixed effects of diet, Zn source, and their interaction, and random effects of parity. Estimated endogenous losses of Zn were used to adjust apparent total tract digestibility (ATTD) to true total tract digestibility (TTTD) of Zn in the four dietary treatment balance periods. There were no differences in Zn concentrations of urine, plasma, colostrum, or milk samples among treatments at any time of the experiment (P > 0.05). Gestating sows fed DDGS/PSZn had improved (P < 0.05) ATTD, TTTD, and overall retention of Zn compared with both Control treatments, with the DDGS/ZnSO4 treatment responses being intermediate. Lactating sows consuming diets without DDGS and supplemented with Zn polysaccharide complex had the greatest (P < 0.05) ATTD, TTTD, and retention of Zn, which were opposite to responses observed in gestation. Furthermore, ATTD, TTTD, and Zn retention for lactating sows consuming DDGS/PSZn were less (P < 0.05) than all other treatments. Overall, zinc digestibility of ZnSO4 and PSZn appears to be differentially influenced by the stage of the reproductive cycle and presence of dietary fiber from DDGS.

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PSIV-8 Effect of Feeding Levucell SB® 10 Titan Advantage and YANG on Performance, Blood Parameters, and Fecal VFA of Nursery Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.300 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 177-178

Author(s):

Huyen Tran ◽

Michael Shields ◽

Roger Arentson ◽

Terry Weeden ◽

Brenda de Rodas

Keyword(s):

Complete Blood Count ◽

Blood Parameters ◽

Nutrient Requirements ◽

Yeast Saccharomyces Cerevisiae ◽

Nursery Pigs ◽

Feeding Program ◽

Yeast Product ◽

And Gender ◽

Dietary Treatments ◽

Effect Of Feeding

Abstract A total of 496 weaned pigs (19 ± 1 d of age) were used in a 3-phase feeding program to evaluate effects of Levucell SB 10 Titan Advantage, a live yeast, Saccharomyces cerevisiae boulardii (LEV; Elanco) and a non-viable yeast product (YANG; Elanco) on performance, complete blood count (CBC), and fecal VFA of nursery pigs. Pigs were blocked by weaning BW and gender and allotted to 44 pens (11–12 pigs/pen) which was assigned to one of 4 dietary treatments (11 pens/treatment). The study was designed as 2 x 2 factorial with LEV and YANG. Pigs were fed to meet or exceed nutrient requirements (NRC, 2012). Blood (d 10 and 22) and fecal samples (d 22) were collected from one pig per pen for CBC and VFA analyses. During d 0–7, feeding LEV or YANG alone improved (P < 0.05) G:F by 20% and 12%, respectively. There was a tendency of LEV*YANG interaction (P < 0.10) where feeding LEV increased (P < 0.05) BW and ADG compared to the control (CON). During d 0–14, feeding YANG with LEV reduced (P < 0.05) BW, ADG, and G:F compared to feeding YANG or LEV alone. Pigs fed LEV had an improved G:F compared to pigs fed CON or YANG with LEV (P < 0.05). During d 21–42, there was a tendency (P < 0.10) for a LEV*YANG effect where pigs fed CON had a lower G:F compared to other treatments. Overall, feeding LEV alone increased (P < 0.01) G:F (2.4%) and numerically increased final BW (0.60 kg) compared to CON. LEV tended to increase blood lymphocytes (P = 0.11) and increased platelets and platelet hematocrit (P < 0.05) compared to CON. There were no differences in fecal VFA measures. The results of this study indicate that feeding LEV increased G:F of nursery pigs.

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Optimal crude protein in diets supplemented with crystalline amino acids fed to high-yielding lactating sows1

Journal of Animal Science ◽

10.1093/jas/skz200 ◽

2019 ◽

Vol 97 (8) ◽

pp. 3399-3414 ◽

Cited By ~ 8

Author(s):

Camilla K Hojgaard ◽

Thomas S Bruun ◽

Peter K Theil

Keyword(s):

Milk Fat ◽

Crude Protein ◽

Dietary Treatment ◽

Dilution Technique ◽

Lactating Sows ◽

Milk Urea ◽

Quadratic Effects ◽

Dietary Treatments ◽

Crystalline Amino Acids ◽

Recommended Level

Abstract The objective of the current study was to determine the requirement of standardized ileal digestible (SID) CP for maximal litter gain in high-yielding lactating sows due to insufficient supply of either His, Leu, Val, Ile, or Phe. The content of SID Lys was formulated at 95% of the recommended level, while that of Met, Met+Cys, Thr, and Trp was formulated at 100% of the recommended level or slightly greater using crystalline AA. A total of 540 parity 1 to 5 sows (L×Y, DanBred, Herlev, Denmark) were included in the study from day 3 after farrowing until weaning at day 26. Sows were allocated to six dietary treatments increasing in SID CP content (96, 110, 119, 128, 137, and 152 g/kg). Litters were standardized to 14 piglets at day 3 ± 2 after farrowing. At day 3 ± 2 after farrowing and at day 26 ± 3, sow BW and back fat, and litter weight were recorded. On a subsample of 72 sows (parity 2 to 4), litters were also weighed at days 10 and 17 ± 3, and milk and blood were sampled at day 3 ± 2 d, and 10, 17 and at 24 ± 3 d in lactation. Sow body pools of protein and fat were determined on the 72 sows at days 3 ± 2 and 26 ± 3 d using the D2O dilution technique. All data were subjected to ANOVA, and to linear and quadratic polynomial contrasts. Variables with quadratic effects or days in milk × treatment interactions were analyzed using linear regression or one-slope linear broken line using the NLMIXED procedure of SAS. Average daily litter gain reached a breakpoint at 125 g SID CP/kg (as-fed). Multiparous sows had a greater litter gain than primiparous sows (3.33 vs. 3.02 kg/d above the breakpoint; P < 0.001) but litter size (13.1 ± 0.1) at weaning were unaffected by dietary treatment (P = 0.62). Sow BW loss was minimized at 102 g SID CP/kg. Concentrations of protein and casein in milk increased linearly with increasing SID CP (P < 0.001). Milk urea reached a minimum at 111–118 g SID CP/kg (P < 0.05) and milk fat a maximum at 116 g SID CP/kg (P < 0.05). In conclusion, 125 g SID CP/kg feed was required to maximize litter gain.

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Effect of supplementing lactation and nursery pig diets with capsaicin on growth performance and gene expression of nursery pigs

Translational Animal Science ◽

10.1093/tas/txab201 ◽

2021 ◽

Author(s):

Eduardo A Rosa-Medina ◽

Pedro E Urriola ◽

Jae C Jang ◽

Christopher D Faulk ◽

Lee J Johnston ◽

...

Keyword(s):

Gene Expression ◽

Growth Performance ◽

Repeated Measures ◽

Mixed Model ◽

Phase 1 ◽

Average Daily Gain ◽

Dietary Treatment ◽

Lactation Period ◽

Lactating Sows ◽

Dietary Treatments

Abstract The objective of this study was to determine the effects of supplementing capsaicin in diets for lactating sows and their offspring on the growth performance and gene expression of pigs post-weaning. Twenty-eight multiparous sows were fed corn-soybean meal-based diets without (n = 14) and with (n = 14) capsaicin (2.5 mg/kg) during a 19-d lactation period. Litters from these sows (n = 288 pigs) were weaned and assigned to 36 blocks (pens) based on maternal dietary treatment and initial body weight (BW) to provide 8 pigs/pen. Blocks were assigned randomly to one of two nursery dietary treatments (control or capsaicin supplemented diets) in a 2 × 2 factorial arrangement of treatments to provide 9 replications per treatment combination. A 3-phase nursery feeding program was used and consisted of feeding phase 1 (weaning to d 7), phase 2 (d 8 to 21), and phase 3 (d 22 to 38) diets post-weaning, without and with 1.0, 1.3, and 1.6 mg capsaicin per kg of diet, respectively. Data were analyzed using a mixed model with the effect of nursery dietary treatment nested within sow lactation treatment, the effect of time with repeated measures, and interactions between treatments and wk post-weaning. On d 38 post-weaning, blood samples were collected from one pig in each pen (n = 36) with BW closest to the pen average for RNA sequencing and gene expression analysis. There were no effects of feeding capsaicin diets to lactating sows and/or their weaned offspring on BW, average daily gain, or average daily feed intake of pigs during the 35-d nursery period. However, pigs weaned from sows fed capsaicin during lactation and continuing to be fed capsaicin diets during the nursery period tended (P = 0.09) to have greater gain:feed (G:F) than pigs fed the other dietary treatments. Furthermore, there was an interaction (P < 0.01) for G:F for dietary treatment and wk post-weaning, where the magnitude of improvement was greater during the first wk post-weaning than subsequent wks. There were a limited number of differentially expressed genes among dietary treatment combinations but the greatest number occurred in offspring from sows that were fed capsaicin during lactation. In conclusion, the combination of feeding capsaicin to sows during lactation and to their offspring after weaning appears to improve gain efficiency for the first wk post-weaning and may alter gene expression to a greater extent than when capsaicin is supplemented only in the nursery diets.

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Physical and Chemical Characteristics of Meats of the Indonesian Indigenous Crossbred Chickens Fed Fermented Mixture of Papaya (Carica papaya L.) Leaf and Seed Meal

International Journal of Veterinary Science ◽

10.37422/ijvs/029 ◽

2020 ◽

Keyword(s):

Crude Protein ◽

Control Diet ◽

Dietary Treatment ◽

Seed Meal ◽

Chemical Characteristics ◽

Breast Meat ◽

Ph Values ◽

Physical And Chemical Characteristics ◽

Dietary Treatments ◽

Physical And Chemical

The objective of this present study was to investigate the effect of feeding fermented mixture of papaya leaf and seed meal (FERM) on the physical and chemical characteristics of meats of the Indonesian indigenous crossbred chicken (IICC). The study was carried out with 300 day-old IICC. The chicks were randomly distributed to five treatment groups, i.e., CONT (control diet based on corn-soybean- diet), FERMA (diet containing 1% FERM), FERMB (2.5% FERM), FERMC (5% FERM) and FERMD (7.5% FERM). Each treatment group consisted of 6 replicates with 10 IICC in each. At week 8, one chick from each replicate was randomly taken and slaughtered. After being de-feathered and eviscerated, samples from breast and thigh meats were obtained. Results showed that the increased levels of FERM was followed by the increased (P<0.05) pH values, moisture and crude protein content of breast meats of the IICC. Dietary incorporation of FERM especially at the level of 2.5% increased (P<0.05) the content of fat in the breast meat of IICC, while further increased levels of FERM did not alter (P>0.05) the fat content of the IICC breast meat. Dietary treatment did not have any effect (P>0.05) on the lightness (L*) values of breast meat of the IICC. The redness (a*) values were higher (P<0.05) in FERMD breast meat than other. FERM diet resulted in lower (P<0.05) yellowness (b*) values in the IICC breast meat. The pH values and moisture content of thigh meat increased (P<0.05) with the increased level of FERM. The WHC decreased (P<0.05) with the elevated levels of FERM in the diets. There was an increase (P<0.05) in crude protein concentration in FERMA as compared to the other meats. Crude fat and ash concentrations in thigh meat were affected (P<0.05) by the treatments. Dietary treatments had no impact (P>0.05) on L* values of thigh meats. Feeding FERM at 7.5% from diets increased (P<0.05) and decreased (P<0.05) the redness and yellowness of meats. In conclusion, dietary inclusion of FERM especially at the level of 7.5% from diets improved the physical and chemical characteristics of the IICC meats.

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Effects of Fumonisin-Contaminated Corn on Growth Performance of 9 to 28 kg Nursery Pigs

Toxins ◽

10.3390/toxins12090604 ◽

2020 ◽

Vol 12 (9) ◽

pp. 604

Author(s):

Zhong-Xing Rao ◽

Mike D. Tokach ◽

Jason C. Woodworth ◽

Joel M. DeRouchey ◽

Robert D. Goodband ◽

...

Keyword(s):

Growth Performance ◽

Linear Response ◽

Animal Feed ◽

Average Daily Gain ◽

Fumonisin B1 ◽

Nursery Pigs ◽

Feed Production ◽

Dietary Treatments ◽

Daily Gain ◽

Effect Of Feeding

Fumonisin contamination in corn is an emerging issue in animal feed production. Fumonisin disrupts the metabolism of sphingolipids and reduces growth performance. This experiment was conducted to determine the effect of feeding fumonisin-contaminated corn on growth performance and sphinganine (SA) to sphingosine (SO) ratios of 9 to 28 kg pigs. A total of 350 pigs, were used with 5 pigs/pen and 14 pens/treatment. Dietary treatments contained fumonisin-contaminated corn (50 mg/kg of fumonisin B1 + B2) blended with low fumonisin corn (10 mg/kg of fumonisin B1 + B2) to provide dietary fumonisin concentrations of 7.2, 14.7, 21.9, 32.7, and 35.1 mg/kg. From day 0 to 28, increasing fumonisin concentration decreased (linear, p < 0.001) average daily gain, average daily feed intake (linear, p = 0.055), and gain:feed ratio (linear, p = 0.016). Although these response criteria tested linear, the greatest reduction in performance was in pigs fed with 32.7 and 35.1 mg/kg of fumonisin (B1 + B2). Increasing fumonisin concentration increased the serum SA:SO ratio (linear, p < 0.001) on day 14 and 28. In summary, for 9 to 28 kg nursery pigs, increasing fumonisin linearly decreased average daily gain and gain:feed ratio. However, despite the linear response, diets containing up to 21.9 mg/kg of fumonisin did not have as dramatic a decrease in growth performance as those fed more than 32.7 mg/kg. Further research is warranted to determine the effect of fumonisin concentrations between 21.9 and 32.7 mg/kg.

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241 Evaluation of a DFM and OA, Alone or in Combination, on Sow Reproductive and Litter Growth Performance

Journal of Animal Science ◽

10.1093/jas/skab054.231 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 137-138

Author(s):

Morgan T Thayer ◽

Jacob A Richert ◽

Karissa N Rulon ◽

Matthew D Asmus ◽

Daniel B Jones ◽

...

Keyword(s):

Fatty Acids ◽

Growth Performance ◽

Bacillus Licheniformis ◽

Reproductive Performance ◽

Body Weight Loss ◽

Short Chain Fatty Acids ◽

Lactating Sows ◽

Sow Lactation ◽

Dietary Treatments ◽

Larger Sample

Abstract Forty-seven sows and their progeny were used to determine if feeding gestating and lactating sows a Bacillus licheniformis direct-fed microbial (DFM), an organic acid blend of medium chain and short chain fatty acids (OA), or in combination improves sow lactation feed and water intake, litter growth, and subsequent reproductive performance. On approximately d80 of gestation, sows were fed one of four diets in a 2 x 2 factorial design: 1) gestation control (CON; 0.55% SID Lysine), 2) CON with DFM (1.6x109 CFU/kg of complete feed), 3) CON with 0.4% OA, 4) CON with both DFM and OA. Dietary treatments were also fed throughout lactation (1.00% SID Lysine) starting on approximately d112 of gestation when sows entered farrowing facility. There was a tendency (P = 0.079) for DFM to decrease the amount of sow body weight loss in lactation by approximately 6% compared to sows not consuming the DFM, likely related to DFM sows numerically (P = 0.124) consuming 8.4% more feed during d7-14 of lactation. Sows fed the OA diets had fewer mummies/litter (P = 0.038) compared to diets not containing OA. Sows fed diets with the DFM gave birth to lighter pigs born alive (P = 0.003) compared to non-DFM fed sows, and a tendency for an interaction (P = 0.092) existed where feeding OA+DFM lessened the decrease in born alive BW. There was an interaction tendency (P = 0.133) where sows fed DFM returned to estrus 22 hours sooner than CON, but only 8 hours sooner when sows were fed the OA+DFM diet. In conclusion, feeding a Bacillus licheniformis DFM to sows may decrease pig born alive weight but reduce sow BW loss through 6.4% more lactation feed intake, quickening the return to estrus. Feeding the OA alone or in combination did not improve sow reproductive and litter growth performance in this study, and may require a larger sample size.

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PSVI-1 Dietary protease supplementation on growth performance and post-weaning diarrhea of nursery pigs

Journal of Animal Science ◽

10.1093/jas/skaa054.337 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 191-192

Author(s):

Jorge Y Perez-Palencia ◽

David A Clizer ◽

Christian Ramirez-Camba ◽

Ryan S Samuel ◽

Crystal L Levesque

Keyword(s):

Growth Performance ◽

Phase 1 ◽

Dietary Treatment ◽

Experimental Period ◽

Phase 2 ◽

Deficient Diet ◽

Nursery Pigs ◽

Feeding Program ◽

Dietary Treatments ◽

Fecal Score

Abstract This experiment evaluated the effect of dietary protease supplementation on pig’s growth performance and post-weaning diarrhea in a 43-d trial. A total of 936 weaned pigs [21 d of age, initial BW 5.87 ± 0.31 kg] were used in a 2 × 2 factorial design (2 nutritional levels × 2 protease levels). The nutritional levels corresponded to a standard feeding program and a reduced feeding program (15% lower SID Lys) and without or with protease supplementation (0.0125% inclusion). Piglets were distributed in 36 pens with 26 pigs per pen and 9 replicates per dietary treatment. Pigs received a common Phase 1 diet and experimental diets in Phase 2, 3, and 4, (0.91, 3.4, 4.5, and 24.0 kg/pig, respectively). Phytase was included at 2,500 U in Phase 2 – 4, all diets met energy and AA:Lys requirements for nursery pigs. Feed intake and body weight were determined every 2 weeks. A pen fecal score assessment was performed at d7, 10, and 14. Performance data were analyzed using the PROC MIXED procedure and fecal score data using the PROC FREQ procedure (SAS Inst., Inc., Cary, NC). No significant interactions (P > 0.05) were observed between dietary treatments. From d15 to 43 and during overall experimental period, pigs fed standard diets had greater BW, ADG, and G:F (P < 0.05) than pigs fed deficient diets (Table 1). On d7, pigs fed deficient diet had more (X2 < 0.05) soft and watery feces, and consequently less normal feces compared to pigs fed standard diets (Figure 1). On d7, 10, and 14, pigs fed diets with protease supplementation had more (X2 < 0.05) normal feces compared to control pigs. Overall, effects of dietary protease supplementation on growth performance were not expressive under the conditions of this study but protease addition benefited intestinal health of nursery pigs.

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285 Effects of dietary MINTREX trace minerals and validation of serum biomarkers in measuring lameness in pigs

Journal of Animal Science ◽

10.1093/jas/skaa054.187 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 110-111

Author(s):

Karen J Wedekind ◽

Ashley Provin ◽

Chelsie Foran ◽

Tom Hampton ◽

Ping Ren ◽

...

Keyword(s):

Dietary Treatment ◽

Cartilage Degradation ◽

Serum Biomarkers ◽

Trace Minerals ◽

Average Score ◽

Bone Degradation ◽

Welfare Issue ◽

Gait Score ◽

Dietary Treatments ◽

Main Effects

Abstract Lameness is a welfare issue and is the most common involuntary reason for culling pigs. Study objectives were to compare objective measures of lameness to gait score and assess effects of dietary chelated trace minerals in finisher pigs. The study was a 2 x 2 factorial arrangement consisting of naturally- occurring lame vs healthy pigs and 2 dietary treatments: 1) metal methionine hydroxy analogue chelate (MMHAC) supplied as MINTREX® Zn-Cu-Mn (Novus International, Inc.) supplemented at 80-10-20 mg/kg diet; and 2) sulfates Zn-Cu-Mn supplemented at 120-20-40 mg/kg diet. The study consisted of 4 groups of pigs (50-70 kg; 8 lame/8 non-lame per group) staggered one month apart. Dietary treatments were fed 53d. Measurements included gait scoring (0-4) and a panel of serum biomarkers (P2CP, CTX2, C2C, CTX1, osteocalcin) and were measured at baseline, d28 and d53. Serum biomarkers included bone synthesis (osteocalcin), bone degradation (CTXI), cartilage synthesis (P2CP), and cartilage degradation (C2C, CTX2) as well as the ratio of synthesis/ degradation for a total of 8 biomarkers. Proc Mixed GLM procedure of SAS was used and means were determined using a Tukey test. The main effects of healthy vs lame and dietary treatment were significant, but no diet x lameness interactions were observed. Lame pigs had elevated CTX2 (P< .01), C2C (P=.02) and tended to have reduced ratio of P2CP:CTX2 (P=.09) compared to healthy pigs. MMHAC increased P2CP (P=.01) and P2CP:CTX2 ratio (P=.03) in both healthy and lame pigs compared to sulfates. These findings demonstrate that biomarkers can distinguish mildly lame pigs (average score of 1) from healthy pigs and lower concentrations of MMHAC vs commercial level of sulfates increased cartilage synthesis and the ratio of cartilage synthesis/degradation in both lame and healthy pigs.

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