scholarly journals 255 Determining the optimal dose of microbial enzyme to enhance fiber digestion

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 92-93
Author(s):  
Colton Althaber ◽  
K P Coffey ◽  
Devan Paulus Compart ◽  
Sarah Shelby ◽  
Melanie Hays ◽  
...  
Keyword(s):  
Dry Matter ◽  
Optimal Dose ◽  
Optimal Dosage ◽  
Fiber Digestion ◽  
Dietary Adaptation ◽  
Dosage Effects ◽  
Microbial Enzyme ◽  
Ad Libitum ◽  
Enzyme Dosage ◽  
Fecal Collection

Abstract Certain enzymes may enhance fiber digestion, but the optimum dosage for ruminants consuming bermudagrass hay is not known. The objective of this study was to determine the optimal dosage of a combination of a protease with Aspergillus oryzae and A. niger fermentation extract blend on fiber digestion and rumen fermentation of bermudagrass hay by sheep. Twenty ewes (30.3 ± 5.29 kg BW) were allocated randomly to 1 of 5 diets (n = 4/treatment). Bermudagrass hay was chopped and offered for ad libitum consumption to ewes housed individually in 1 × 1.5-m pens with plastic-coated grate flooring. Diets were supplemented with 0.25% BW of soybean meal and either no enzyme or 1.5, 3, 4.5, or 6 g of a mixture of a protease with A. oryzae and A. niger fermentation extracts in a calcium carbonate carrier. A 14-d dietary adaptation was followed by 7 d of total fecal collection. On d 21, 3 rumen samples per ewe were taken at 4 h intervals for analysis of pH and VFA concentrations. Data were analyzed using the MIXED procedure of SAS and orthogonal linear and quadratic contrasts were used to assess enzyme dosage effects. Dry matter and ADF digestibility increased quadratically (P < 0.05), and NDF and OM digestibility tended (P ≤ 0.07) to increase quadratically across dosages. Ruminal acetate concentrations decreased quadratically and ruminal propionate increased quadratically (P < 0.05) with increasing enzyme dosage (P < 0.05). Total VFA concentrations were not affected by dosage (P = 0.71), but the acetate:propionate ratio decreased quadratically (P < 0.05) with increasing enzyme dosage. In conclusion, a protease and A. oryzae and A. niger fermentation extract blend increased fiber digestion, but dosages over 3 g of enzyme did not further enhance DMD or DMI.

scholarly journals 265 Determination of microbial enzyme efficacy on fiber digestion of bermudagrass hay supplemented with either DDGS or corn

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 93-93
Author(s):  
Colton Althaber ◽  
K P Coffey ◽  
Devan Paulus Compart ◽  
Sarah Shelby ◽  
Melanie Hays ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Rumen Fermentation ◽  
The Other ◽  
Fiber Digestion ◽  
Orthogonal Contrasts ◽  
Dietary Adaptation ◽  
Microbial Enzyme ◽  
Ad Libitum ◽  
Fecal Collection

Abstract Certain enzymes have improved fiber digestion of low-quality forages. Our objective was to determine the efficacy of a combination of a protease and Aspergillus oryzae and A. niger fermentation extracts on fiber digestion and rumen fermentation of bermudagrass hay supplemented with different energy concentrates. Bermudagrass hay was chopped and offered for ad libitum consumption to 20 ewes per study (33.1 ± 10.65 kg BW; Exp. 1; 36.0 ± 11.10 kg BW; Exp. 2) housed individually in 1 × 1.5-m pens (n = 5/trt). In Exp. 1, hay supplemented with DDGS at 0.25% BW, 1% BW, 1% BW + 2.3 g enzyme, or 1% BW + 3.5 g enzyme. In Exp. 2, hay was supplemented with cracked corn at the same levels as used in Exp. 1. Each experiment consisted of a 14-d dietary adaptation followed by 7 d of total fecal collection. On d 21, rumen samples were taken at 3 h intervals from each ewe for analysis of pH and VFA concentrations. Data were analyzed using PROC MIXED of SAS and orthogonal contrasts compared 0.25% vs. 1% BW, no enzyme vs. 2.3 g/d enzyme and 2.3 g/d vs. 3.5 g/d enzyme. In Exp. 1 NDF digestibility was greater (P < 0.05) from 1% vs. 0.25% BW supplement. Ruminal acetate and the acetate:propionate ratio were greatest (P < 0.05) from 0.25% BW vs. the other diets. Exp. 2, DM and OM intake were greater (P < 0.05) from the 1% BW diets and tended (P = 0.08) to be greater from 1% BW alone vs. 1% BW + 2.3 g enzyme. Ruminal acetate concentrations were greatest (P < 0.05) from 0.25% BW. Therefore, the blend of A. oryzae and A. niger fermentation extracts and a protease was not effective under the conditions of this study.

UTILIZATION OF GRASS SILAGE BY CATTLE: EFFECT OF BARLEY AND FISHMEAL SUPPLEMENTS

1985 ◽  
Vol 65 (4) ◽  
pp. 897-903 ◽  
Author(s):  
D. M. VEIRA ◽  
M. IVAN ◽  
G. BUTLER ◽  
J. G. PROULX
Keyword(s):  
Formic Acid ◽  
Nitrogen Content ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Beef Steers ◽  
Large Reduction ◽  
High Nitrogen Content ◽  
High Nitrogen ◽  
Grass Silage ◽  
Ad Libitum

Following weaning at 6–7 mo of age, 36 beef steers were used to determine production responses when grass silage was supplemented with barley or fishmeal. The silage was made from direct-cut, formic- acid-treated grass harvested from a mixed sward and had a high nitrogen content but poor fermentation characteristics. The silage was fed ad libitum for 98 days either alone or supplemented with 500 g fishmeal or 500 g barley per day. Both fishmeal and barley increased total dry matter intake (P < 0.01) by an amount equivalent to the quantity of supplement offered but had no effect on silage intake (P > 0.05). Steers fed the fishmeal grew substantially faster than either the barley (0.53 kg/day) or unsupplemented (0.54 kg/day) groups (P < 0.01). Fishmeal supplementation resulted in a large reduction (35%) in the amount of feed required per kilogram of gain. Key words: Cattle, grass silage, fishmeal, growth

Factors limiting the intake of feed by sheep. I. The significance of palatability, the capacity of the alimentary tract to handle digesta, and the supply of glucogenic substrate.

1966 ◽  
Vol 17 (6) ◽  
pp. 939 ◽  
Author(s):  
RH Weston
Keyword(s):  
Acetic Acid ◽  
Propionic Acid ◽  
Polyvinyl Chloride ◽  
Dry Matter ◽  
Alimentary Tract ◽  
Control Period ◽  
Feed Consumption ◽  
Ad Libitum

Voluntary feed consumption (VFC) decreased when a quantity of the diet being consumed by the sheep was introduced into the rumen. On diets of lucerne hay, lucerne hay plus maize, wheaten hay, and wheaten straw, the decreases in VFC ranged from 90 to 110% of the quantity introduced intraruminally when this quantity varied from 20 to 60% of the VFC during control periods. When sheep consumed wheaten straw ad libitum, and a quantity of coarsely ground wheaten straw equal to 140% of the VFC during a control period was given intraruminally, VFC ceased and digesta accumulated in the rumen. VFC decreased when indigestible materials were introduced into the rumen. The voluntary consumption of lucerne hay decreased by 15% when sawdust was introduced into the rumen at a rate equivalent by weight to 17% of the VFC during a control period ; decreases of 7 and 9% were found when finely ground polyvinyl chloride was introduced at rates equivalent by weight to 25 and 50% respectively of VFC during control periods. The voluntary consumption of wheaten hay decreased by 16 and 19% when sawdust was introduced into the rumen at rates equivalent by weight to 25 and 18% respectively of VFC during control periods. Intraruminal infusions of acetic acid, propionic acid, or a mixture of acetic, propionic, and butyric acids providing 290–510 kcal of energy daily were accompanied by decreases in the voluntary consumption of lucerne hay and wheaten hay; the decreases ranged from 0.29 to 0.86 g dry matter per kcal of energy infused; similar decreases were obtained when propionic acid was infused into the abomasum of sheep given lucerne hay. The intake of lucerne hay was 9% higher when given alone than when supplemented with 6% propionic acid. The results are discussed in relation to factors limiting the intake of lucerne hay and wheaten hay.

Effect of physical processing on in situ degradation of barley in the caecum of ponies

1998 ◽  
Vol 1998 ◽  
pp. 127-127
Author(s):  
B M L McLean ◽  
J J Hyslop ◽  
A C Longland ◽  
D Cuddeford
Keyword(s):  
Sample Size ◽  
Dry Matter ◽  
Cereal Grains ◽  
Processing Methods ◽  
Grass Hay ◽  
Physical Processing ◽  
In Situ Degradation ◽  
Ad Libitum ◽  
Fixed Times

Cereal grains are often subjected to physical processing before being fed to equids. However, little information is available on how physical processing of cereals affects degradation dynamics in equids. This experiment examines the effect of two physical processing methods (micronisation and extrusion) on in situ degradation of barley in the caecum of poniesThree caecally fistulated mature Welsh-cross pony geldings (approx. LW 270kg) were offered ad libitum grass hay plus minerals. Incubation bags (monofilament polyester 6.5 x 20cm, 41μm pores, 16mg/cm2 sample size) containing either unprocessed barley (UB), micronised barley (MB) or extruded barley (EB) were incubated in the caecum for fixed times according to both a forward (0, 2, 4, 6, 12, 8, 24, 48h) and reverse (48, 24, 8, 4, 12, 6, 2, 0h) incubation sequence. For each feedstuff residues from each time were bulked within pony and across incubation sequence for subsequent analysis of dry matter (DM) and starch (STC). Degradation profiles were fitted to the DM and STC disappearance data according to Ørskov and McDonald (1979).

THE EFFECT OF INGESTED CHLORTETRACYCLINE ON SOME HYDROLASES AND ORGANS ASSOCIATED WITH THE DIGESTIVE PROCESS IN GROWING PIGS: II. PROTEASE, AMYLASE, AND GELLULASE ACTIVITY OF THE CONTENTS OF THE SMALL INTESTINE AND OF THE CECUM; WEIGHTS OF SMALL INTESTINE AND MUCOSA

1957 ◽  
Vol 35 (3) ◽  
pp. 195-202 ◽  
Author(s):  
M. A. A. Vonk ◽  
L. W. McElroy ◽  
R. T. Berg
Keyword(s):  
Small Intestine ◽  
Dry Matter ◽  
Cellulase Activity ◽  
Dry Weight ◽  
Growing Pigs ◽  
Weanling Pigs ◽  
Intestinal Contents ◽  
Wet Weight ◽  
The Mean ◽  
Ad Libitum

Four treatments, involving differences in method of feeding and interval between last feed and slaughter, were employed in a study of the effect of dietary chlortetracycline on protease, amylase, and cellulase activity in the intestinal and cecal contents of 16 pairs of weanling pigs. Most consistent results were obtained with six pairs which were limited pair-fed except for the final feeding during which feed was available ad libitum for a 4-hour period ending 18 hours before slaughter. The mean total activities of all three hydrolases in the contents of the small intestines and of the ceca of the antibiotic-fed animals of these six pairs were significantly greater than in those of the control animals. Expressed as activity per gram dry matter of intestinal contents, significantly higher values for protease and amylase, but not for cellulase, were observed in the pigs that had received chlortetracycline. When the combined results obtained from all 16 pairs of the experimental animals were analyzed, the results showed that on a basis of activity per gram dry matter of intestinal contents, ingested chlortetracycline was associated with significant increases in amylase and cellulase but not in protease activity. Protease, amylase, and cellulase activities per gram dry matter of cecal contents were higher for pigs fed the antibiotic than for their controls. The mean wet weight of the empty small intestine and the mean dry weight of the mucosa scraped from the anterior 3-meter section of the small intestine were lower for the chlortetracycline-fed animals, but the differences were not statistically significant.

Effects of nutrition in late pregnancy on subsequent milk production in Ewes

1970 ◽  
Vol 12 (1) ◽  
pp. 23-36 ◽  
Author(s):  
T. T. Treacher
Keyword(s):  
Milk Production ◽  
Dry Matter ◽  
Live Weight ◽  
Late Pregnancy ◽  
Lower Content ◽  
Weight Changes ◽  
Total Birth ◽  
Weight Gains ◽  
Ad Libitum ◽  
Voluntary Intake

SUMMARY1. Scottish Half-bred ewes carrying twin foetuses were fed individually to make live-weight gains in the last six weeks of pregnancy of (1) 20%, (2) 10% and (3) 0% of their live weight in week 14 of pregnancy. In lactation the ewes were fed ad libitum. The lambs were removed 12 to 16 hr after parturition and the ewes were machine-milked twice daily for the first six weeks of lactation.2. Total birth weights per ewe of twin lambs from the treatments were (1) 10·10 kg, (2) 9·44 kg and (3) 8·18 kg and differed significantly.3. The level and pattern of voluntary intake in lactation did not differ significantly between the treatments. Total dry-matter intakes in the six weeks of lactation were (1) 121·9 kg (2) 105·9 kg and (3) 109·5 kg.4. The pregnancy treatments affected the level of milk production and the shape of lactation curves. The total yields in the first six weeks of lactation were (1) 58·8 kg, (2) 43·5 kg and (3) 26·9 kg. Higher contents of fat and protein and the lower content of lactose in the milk from treatment-3 ewes on days 1 and 3 of lactation indicated a slower onset of lactation in these ewes. Between days 7 and 35 of lactation the contents of fat and SNF were lowest on treatment 3 but the differences were not significant.5. The live-weight changes in lactation, which were in inverse order to the gains in late pregnancy, were (1) 3·4 kg, (2) 5·5 kg and (3) 9·5 kg.

scholarly journals NUTRITIVE VALUE OF FRESH AND DRIED MARROW-STEM KALE HARVESTED AT TWO DIFFERENT PERIODS

1973 ◽  
Vol 53 (2) ◽  
pp. 257-263 ◽  
Author(s):  
G. PELLETIER ◽  
E. DONEFER
Keyword(s):  
Brassica Oleracea ◽  
Dry Matter ◽  
Nutritive Value ◽  
Chemical Analyses ◽  
High Quality ◽  
Dry Matter Digestibility ◽  
Gross Energy ◽  
Plant Constituents ◽  
Ad Libitum ◽  
Voluntary Intake

Three yearling Cheviot × Suffolk ewes and three Columbia wethers were confined in metabolic cages and fed ad libitum fresh and dried marrow-stem kale (Brassica oleracea var. acephala D.C.) harvested in mid- and late October. Chemical analyses of the forages indicated no marked effects on any of the plant constituents due to drying or advancing maturity. Digestibility measurements demonstrated that marrow-stem kale (MSK) was a highly digestible material with an average dry matter digestibility of 78%. The fresh forages had higher (P < 0.05) dry matter and gross energy digestibility values than the dried form. Dry matter digestibility coefficients were higher (P < 0.05) for late October as compared with the earlier harvest. A trend toward higher (P > 0.05) voluntary intake values due to drying maturity was observed with an average relative intake of 80%. The nutritive value index averaged 65, thus indicating a digestible energy intake similar to a high-quality (legume) forage.

Effect of intraruminal infusion of urea on the response in voluntary food intake by sheep

1985 ◽  
Vol 36 (3) ◽  
pp. 483 ◽  
Author(s):  
JK Egan ◽  
PT Doyle
Keyword(s):  
Organic Matter ◽  
Food Intake ◽  
Dry Matter ◽  
Block Design ◽  
Ammonia Nitrogen ◽  
Dry Matter Intake ◽  
Plant Cell Walls ◽  
Randomized Block Design ◽  
Ad Libitum ◽  
Ad Libitum Intake

Six mature Merino sheep received three treatments in a randomized block design experiment. The treatments were: chopped oaten hay diet at 90% of ad libitum intake without urea (L); the ciet offered at the same level as for L with urea infused into the rumen at 11.5 g kg-1 dry matter intake (LU); and the diet offered at 90% of the ad libitum intake achieved with urea infused at 11.5 g kg-1 dry matter intake (HU). Sheep given HU consumed 37% more (P < 0.01) organic matter (OM) than those fed L or LU, but the apparent digestibility of OM did not vary (59.2-61.8%) between treatments. The addit onal food consumption was associated with c. 20% increase (P < 0.05) in the weight of OM in the reticulorumen and significantly higher (by 10-35%; P < 0.05) fractional outflow rates of most dietary and microbial constituents of digesta. The fractional digestion rate of potentially digestible plant cell walls was not affected by urea, but the flow of microbial non-ammonia nitrogen from the abomasum was enhanced (L, 7.0; LU, 8.2; HU, 12.5 g day-1; P < 0.05). The results of this study suggest that the stimulatory effect of urea upon food intake was associated with the provision of additional microbial protein for digestion in the intestines, rather than changes in the rate or extent of organic matter fermentation in the reticulorumen.

Rumen degradation of straw 4. Selection and degradation of morphological components of barley straw by sheep

1988 ◽  
Vol 47 (1) ◽  
pp. 105-110 ◽  
Author(s):  
P. K. Bhargava ◽  
E. R. Ørskov ◽  
T. K. Walli
Keyword(s):  
Leaf Blade ◽  
Dry Matter ◽  
Leaf Sheath ◽  
Practical Significance ◽  
Barley Straw ◽  
Rumen Degradation ◽  
Whole Plant ◽  
Ad Libitum ◽  
The Mathematical Model ◽  
Selection Of

AbstractExperiments were made to study the proportion, chemical composition, and rumen degradability of the morphological components of barley straw (Corgi variety) and to study the selection of these components by sheep. The proportions in the harvested straw dry matter (DM) as leaf blade, leaf sheath, stem and chaff were 128, 314, 500 and 58 g/kg DM, respectively. The different components of straw on analysis proved to have very different concentrations of nitrogen and neutral-detergent cellulase digestibility. The leaf blades had the highest and the stems the lowest values. The degradabilities of DM in the components and in the whole straw were determined by measuring DM loss from samples incubated in nylon bags for various periods in the rumen of sheep. Responses were measured using the mathematical model p = a+b (1–e−ct) where p is DM loss, (a+b) potential degradability, c the rate constant of DM loss and t is the time of incubation. DM losses decreased in the order leaf blades > leaf sheath > whole plant > chaff > stems. Leaf blades also had the highest potential degradability and rate of degradation.In another trial, five sheep were offered unchopped barley straw ad libitum. There were five treatment periods in which sheep were allowed to leave uneaten proportionately 0·2, 0·3, 0·4, 0·5 and 0·7 of the straw on offer for assessing the animal's selection of the morphological components of that straw. The amount of leaf blade in the material consumed increased in largely a linear (P < 0·01) fashion with the amount of excess allowance. The proportion of stem eaten varied conversely. The selection of leaf sheath was less apparent. Little stem was consumed until the proportion of leaf blade in the food available decreased below proportionately 0·4. The practical significance of the study is discussed.

scholarly journals Performance and digestibility in feedlot lambs fed hay based diets

2016 ◽  
Vol 38 (4) ◽  
pp. 425
Author(s):  
Euclides Reuter de Oliveira ◽  
Flávio Pinto Monção ◽  
Andréa Maria de Araújo Gabriel ◽  
Alexandre Rodrigo Mendes Fernandes ◽  
Lais Valenzuela Moura ◽  
...  
Keyword(s):  
Chromium Oxide ◽  
Dry Matter ◽  
Cynodon Dactylon ◽  
Block Design ◽  
Evaluation Methods ◽  
Daily Weight Gain ◽  
Feed Conversion ◽  
Dry Matter Digestibility ◽  
Feedlot Lambs ◽  
Fecal Collection

This study aimed to evaluate the performance of feedlot lambs fed diets formulated with hay of different genotypes of Cynodon spp. and to compare the dry matter digestibility of diets using different evaluation methods (total fecal collection and chromium oxide). Suffolk crossbred neutered male lambs (n=30), with an average age of 90 days and average body weight of 21.5 kg ± 1.6. Diets were formulated using a standard concentrate and hay of the following genotypes of Cynodon dactylon: Jiggs, Vaquero, Tifton 68, Coast-Cross, Tifton 85 and Russell, in a 60:40 forage concentrate ratio, composing the treatments distributed in a randomized block design in a factorial 5 x 2 arrangement of 5 diets and 2 digestibility evaluation methods. Animals receiving Vaquero hay showed dry matter intake of 18.3; 16.9; 25.6; 20.7 and 24.2% higher than those fed hay of Jiggs, Tifton 68, Coast Cross, Tifton 85 and Russell, respectively. There was no difference between diets for digestibility using total fecal collection, chromium oxide and final weight of animals. Jiggs hay based diets allowed greater daily weight gain and better feed conversion. 

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