scholarly journals PSVII-27 Effects of dietary near ideal amino acid profile on amino acid utilization efficiency for milk production in sows under thermoneutral or heat stress conditions

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 358-358
Author(s):  
Sai Zhang ◽  
Jay Johnson ◽  
Nathalie Trottier
Keyword(s):  
Heat Stress ◽  
Amino Acid ◽  
Milk Production ◽  
Milk Yield ◽  
Amino Acid Profile ◽  
Utilization Efficiency ◽  
Body Protein ◽  
Amino Acid Utilization ◽  
Lactating Sows ◽  
Protein Mobilization

Abstract The objective was to estimate essential AA (EAA) utilization efficiency for milk production in sows (n = 24) fed control (19.6% CP) or reduced CP (RCP; 14.2% CP) diets under thermoneutral (TN; 21±1.5°C) or cycling heat stress (HS; 32±1.5°C daytime and 24±1.5°C nighttime) conditions. We hypothesized that RCP fed lactating sows with supplemental AA have increased EAA efficiency for milk production under TN and HS conditions. Diets contained 0.90% SID Lys and 2,580 kcal/kg. Sow BW and backfat were recorded on d 1 and 21 to estimate body protein mobilization (BPM). Piglet BW was recorded on d 1 and 21 to estimate milk yield. Amino acid efficiencies were calculated based on milk AA output relative to SID AA intake corrected for AA contribution from BPM. During HS, N, Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, and Val efficiency for milk production did not differ between sows fed control (42, 26, 44, 36, 39, 49, 52, 32, 50, 43 and 44%, respectively) and RCP (56, 39, 51, 49, 56, 47, 42, 39, 50, 59 and 41%, respectively) diets. Compared to control sows, RCP sows had greater (P < 0.05) efficiency of Arg (30% vs. 61%) under TN condition. Efficiencies of His, Ile, Leu, Phe, and Trp tended to be greater (P = 0.07, 0.06, 0.06, 0.07 and 0.06, respectively) in RCP compared to control sows (50, 41, 44, 36, and 50% vs. 74, 67, 75, 54 and 78%, respectively). Efficiencies of Lys, Met, Thr, Trp and Val did not differ between control and RCP sows (56, 59, 57, and 50% vs. 67, 59, 69 and 57%, respectively). In summary, an RCP diet improved efficiency of N, Arg, His, Ile, Leu, Phe, and Trp for milk production in lactating sows under TN but not HS conditions.

164 Feeding a Diet with a Near Optimal Amino Acid Profile Improves Energy Utilization for Milk Production in Lactating Sows.

2018 ◽  
Vol 96 (suppl_2) ◽  
pp. 87-87
Author(s):  
S Zhang ◽  
J S Johnson ◽  
M Qiao ◽  
J Liesman ◽  
N L Trottier
Keyword(s):  
Amino Acid ◽  
Milk Production ◽  
Amino Acid Profile ◽  
Energy Utilization ◽  
Lactating Sows

scholarly journals Feeding a reduced protein diet with a near ideal amino acid profile improves amino acid efficiency and nitrogen utilization for milk production in sows1,2

2019 ◽  
Vol 97 (9) ◽  
pp. 3882-3897 ◽  
Author(s):  
Sai Zhang ◽  
Mu Qiao ◽  
Nathalie L Trottier
Keyword(s):  
Amino Acid ◽  
Milk Production ◽  
Nitrogen Retention ◽  
Amino Acid Profile ◽  
Utilization Efficiency ◽  
Protein Diet ◽  
Biological Efficiency ◽  
Lactation Performance ◽  
Total N ◽  
Peak Lactation

Abstract Fifty-four lactating multiparous Yorkshire sows were used to test the hypothesis that feeding a reduced protein diet with a near ideal AA (NIAA) profile increases the biological utilization efficiency of N and essential AA (EAA) for milk production in part as a result of reduced dietary Leu concentration. Sows were fed 1 of 3 isocaloric diets containing the following concentration of CP (% as-fed, analyzed): 18.74 (Control: CON), 13.78 (Optimal: OPT), and 14.25 (Optimal + Leu: OPTLEU). The OPT and OPTLEU diets contained the same concentration of crystalline AA (CAA) to meet requirements of the limiting AA. Crystalline Leu was added to OPTLEU to contain the same SID Leu concentration as that of CON. Sows were weighed on days 1 and 21 of lactation and piglets on days 1, 4, 8, 14, 18, and 21 of lactation. Nitrogen retention was measured for 48 or 72 h between days 4 and 8 (early) and days 14 and 18 (peak) of lactation. Sow BW change and ADFI did not differ between diets. Litter growth rate (LGR) during early lactation did not differ between diets. At peak lactation, LGR was higher in sows fed OPT compared with CON (P < 0.05) and lower in sows fed OPTLEU compared with OPT (P < 0.05). In early and peak lactation, total N retention, and milk N output efficiency were greater in OPT (P < 0.01) and OPTLEU (P < 0.05) than CON. Compared with CON, overall biological efficiency of N, Arg, His, Ile, Leu, Phe, and Trp were greater (P < 0.05), whereas those of Lys, Met, Thr, and Val did not differ in sows fed OPT and OPTLEU, except for Leu which did not differ between OPTLEU and CON. Compared with OPT, only Leu and Met efficiency were lower (P < 0.01) and tended to be lower (P = 0.10), respectively, in sows fed OPTLEU. Reducing CP with a NIAA profile to attain the minimum Leu requirement maintained overall lactation performance, improved utilization efficiency of N, Arg, His, Ile, Leu, Phe + Tyr, and Trp for milk production, and maximized efficiency of Ile, Leu, Lys, Met + Cys, Phe + Tyr, Thr, Trp, and Val. Addition of Leu did not reduce N and EAA utilization efficiency. This study provides revised and novel maximum biological efficiency value (MBEV) for Ile (65.4), Leu (75.1), Lys (63.2), Met + Cys (78.2), Phe + Tyr (69.5), Thr (71.0), Trp (70.1), and Val (57.0). These MBEV can be used to more accurately predict the requirement for those AA during lactation.

scholarly journals 84 Improving dietary amino acid balance reduces heat production in lactating sows exposed to heat stress

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 47-48
Author(s):  
Sai Zhang ◽  
Jay S Johnson ◽  
Nathalie L Trottier
Keyword(s):  
Heat Stress ◽  
Amino Acid ◽  
Gas Exchange ◽  
Positive Pressure ◽  
Metabolic Heat ◽  
Lactating Sows ◽  
Dietary Amino Acid ◽  
Conventional Diet ◽  
Diet Control ◽  
Amino Acid Balance

Abstract We hypothesized that lactating sows fed a reduced CP (RCP) diet with supplemental AA to improve AA balance produce less metabolic heat (HP) compared with feeding a conventional diet (control). The objective was to measure HP of sows (n = 24) fed control (19.6% CP) or RCP (14.2% CP) under thermoneutral (TN, 21 ± 1.5°C) or cycling heat stress (HS, 32 ± 1.5°C daytime and 24 ± 1.5°C nighttime). Diets contained 0.90% SID Lys and 2,580 kcal/kg. Positive pressure indirect calorimeters were used to measure gas exchange in individual sows with litters (sow + litter), and individual piglets on lactation d 4, 8, 14 and 18, and HP determined overnight (1900–0700) and during daytime (0700–1900). Sow and litter weights were recorded on d 1, 10 and 21. Sow HP was calculated by subtracting litter HP from sow + litter HP based on BW0.75. Model included the fixed effect of diet, random effects of block and sow, sow feed intake as a regression variable and day as the repeated measurement. Compared to control, HP (kcal/h·kg0.75) of RCP was lower (P < 0.01) overnight (5.25 vs. 5.96 ± 0.38), during daytime (6.33 vs. 6.94 ± 0.23), and over 24-h (5.78 vs. 6.46 ± 0.24) under HS. Under TN, HP of RCP tended to differ (P = 0.107) overnight (5.20 vs. 5.90 ±0.57) and over 24-h (5.93 vs. 6.48 ± 0.50) and did not differ (P = 0.190) during daytime (6.66 vs. 7.06 ± 0.52). Under HS, lactation day affected HP (P < 0.05) overnight and over 24-h, with HP increasing from d 1 to 14 and decreasing from d 14 to 18. Under TN, day of lactation did not affect sow HP. Feeding RCP diet to lactating sows decreased HP, and this effect was more pronounced in sows housed under HS.

scholarly journals Transcriptome Functional Analysis of Mammary Gland of Cows in Heat Stress and Thermoneutral Condition

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1015 ◽  
Author(s):  
Shuangming Yue ◽  
Zhisheng Wang ◽  
Lizhi Wang ◽  
Quanhui Peng ◽  
Bai Xue
Keyword(s):  
Functional Analysis ◽  
Immune Response ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Milk Production ◽  
Milk Yield ◽  
Molecular Mechanisms ◽  
Mammary Glands ◽  
Differentially Expressed

Heat stress (HS) exerts significant effects on the production of dairy animals through impairing health and biological functions. However, the molecular mechanisms related to the effect of HS on dairy cow milk production are still largely unknown. The present study employed an RNA-sequencing approach to explore the molecular mechanisms associated with a decline in milk production by the functional analysis of differentially expressed genes (DEGs) in mammary glands of cows exposed to HS and non-heat-stressed cows. The results of the current study reveal that HS increases the rectal temperature and respiratory rate. Cows under HS result in decreased bodyweight, dry matter intake (DMI), and milk yield. In the current study, a total of 213 genes in experimental cow mammary glands was identified as being differentially expressed by DEGs analysis. Among identified genes, 89 were upregulated, and 124 were downregulated. Gene Ontology functional analysis found that biological processes, such as immune response, chaperone-dependent refolding of protein, and heat shock protein binding activity, were notably affected by HS. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis found that almost all of the top-affected pathways were related to immune response. Under HS, the expression of heat shock protein 90 kDa beta I (HSP90B1) and heat shock 70 kDa protein 1A was upregulated, while the expression of bovine lymphocyte antigen (BoLA) and histocompatibility complex, class II, DRB3 (BoLA-DRB3) was downregulated. We further explored the effects of HS on lactation-related genes and pathways and found that HS significantly downregulated the casein genes. Furthermore, HS increased the expression of phosphorylation of mammalian target of rapamycin, cytosolic arginine sensor for mTORC1 subunit 2 (CASTOR2), and cytosolic arginine sensor for mTORC1 subunit 1 (CASTOR1), but decreased the phosphorylation of Janus kinase-2, a signal transducer and activator of transcription factor-5. Based on the findings of DMI, milk yield, casein gene expression, and the genes and pathways identified by functional annotation analysis, it is concluded that HS adversely affects the immune function of dairy cows. These results will be beneficial to understand the underlying mechanism of reduced milk yield in HS cows.

scholarly journals The effect of cattle breed, season and type of diet on nitrogen fractions and amino acid profile of raw milk

2013 ◽  
Vol 56 (1) ◽  
pp. 709-718 ◽  
Author(s):  
L. Křížová ◽  
O. Hanuš ◽  
P. Roubal ◽  
J. Kučera ◽  
S. Hadrová
Keyword(s):  
Amino Acid ◽  
Milk Yield ◽  
Cattle Breed ◽  
Raw Milk ◽  
Amino Acid Profile ◽  
The Other ◽  
Nitrogen Fractions ◽  
Milk Samples ◽  
Bulk Milk

Abstract. The aim of the study was to describe the differences in nitrogen fractions and deepen the knowledge in the amino acid profile of raw milk affected by the breed of cattle, season and type of feeding. The study was conducted from June 2005 to February 2007 on 64 bulk milk samples collected from eight herds consisting of Czech Fleckvieh (four herds) and Holstein (four herds) breed. One half of the herds of each breed was grazed while the other half was not. Samples were collected twice in winter and twice in summer. The effect of the breed resulted in differences in milk yield that was lower in Czech Fleckvieh (5 385.50 kg) than in Holstein (7 015.15 kg, P<0.05). The content of nitrogen fractions was higher in Czech Fleckvieh than in Holstein (P<0.05). No effect of the breed on the amino acid profile of milk was observed except on the concentration of Glu (P<0.05). The effect of the season was demonstrated in the decrease of the concentrations of nitrogen fractions and Met during summer in comparison to winter (P<0.05). The effect of the type of feeding resulted in lower milk yield (5 197.50 and 7 203.75 kg) and lower concentrations of nitrogen fractions in grazed herds compared to non-grazed herds (P<0.05), respectively. Furthermore, the amino acid profile of milk differed significantly between grazed and non-grazed herds (P<0.05).

scholarly journals Effects of Herbal Adaptogen Feed-Additive on Growth Performance, Carcass Parameters, and Muscle Amino Acid Profile in Heat-Stressed Modern Broilers

2021 ◽  
Vol 12 ◽  
Author(s):  
Elizabeth S. Greene ◽  
Clay Maynard ◽  
Casey M. Owens ◽  
Jean-François Meullenet ◽  
Sami Dridi
Keyword(s):  
Body Weight ◽  
Heat Stress ◽  
Amino Acid ◽  
Growth Performance ◽  
Feed Intake ◽  
Control Diet ◽  
Amino Acid Profile ◽  
Poultry Production ◽  
Carcass Parameters ◽  
Cyclic Heat

Heat stress has strong adverse effects on poultry production and, thereby, threats its sustainability, which energized scientists to search for innovative and effective solutions. Here, we undertook this study to evaluate the effects of in-feed herbal adaptogen (stress response modifier) supplementation on growth performances, meat quality, and breast amino acid profile in chronic cyclic heat-stressed broilers. Day-old male Cobb 500 chicks (n = 720) were randomly assigned, in environmental chambers (n = 12, 24 pens), to three diet-treatments: a three-phase corn-soybean based diet fed as such (Control, C), or supplemented with the herbal adaptogen at 500 g/1000 kg control diet (NR-PHY-500) or at 1 kg/1000 kg control diet (NR-PHY-1000). From d29 to d42, birds from 9 chambers were exposed to cyclic heat stress (HS, 35°C from 9:30 am-5:30 pm), however, the rest of the chamber were maintained at thermoneutral conditions (24°C, TN), which creates 4 experimental groups: C-TN, C-HS, NR-PHY-500HS, and NR-PHY-1000HS (6 pens/group, 168 birds/group). HS altered growth performance via depression of feed intake and body weight. Adaptogen supplementation stimulated feed intake and averaged 65.95 and 83.25 g better body weight and 5 and 10 points better FCR at low and high dose, respectively, compared to heat-stressed birds. This increase in body weight was mirrored in enhanced weights of body parts (breast, tender, wings, and legs). Adaptogen supplementation modulated also breast amino acid profile, pH, color, and quality. Together, these data suggested that adaptogen supplementation could be a promising solution to alleviate heat stress, however further in-depth investigation for its mode of action and its underlying mechanisms are warranted.

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