scholarly journals Bioavailability of valine in spray-dried L-valine biomass is not different from that in crystalline L-valine when fed to weanling pigs1

2019 ◽  
Vol 97 (10) ◽  
pp. 4227-4234 ◽  
Author(s):  
Maryane S F Oliveira ◽  
John K Htoo ◽  
J Caroline González-Vega ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that Val from a spray dried L-Val fermentation biomass (Val-FB; 64.4% L-Val) has a bioavailability of 100% relative to Val from L-Val (98% L-Val) when fed to weanling pigs. A Val-deficient basal diet containing 0.63% standardized ileal digestible (SID) Val was formulated. Six additional diets were prepared by supplementing the basal diet with 0.08%, 0.17%, or 0.25% L-Val or 0.12%, 0.25%, or 0.37% Val-FB to create experimental diets from both Val sources that contained 0.71%, 0.79%, or 0.87% SID Val. Two hundred twenty-four weaned pigs (6.87 ± 0.64 kg initial BW) were allotted to a randomized complete block design with 7 diets, 4 pigs per pen, and 8 replicate pens per diet. Diets were fed for 20 d. At the conclusion of the experiment, a blood sample from 1 pig per pen was analyzed for blood urea nitrogen (BUN) and plasma free AA. A linear regression model was used to estimate the relative bioavailability (RBV) of Val in Val-FB relative to Val from L-Val. Results indicated that the final BW and ADG were greater (P < 0.01) for pigs fed diets supplemented with Val-FB than pigs fed diets supplemented with L-Val. The ADFI decreased (linear, P ≤ 0.01), whereas G:F increased (linear, P < 0.01) by increasing inclusion of both Val sources in the diets. Regardless of source of dietary Val, BUN values were reduced (linear and quadratic, P < 0.01) as the concentration of Val in the diet increased. Pigs fed diets supplemented with L-Val had increased (linear and quadratic, P ≤ 0.05) concentrations of Val and Arg in plasma, and plasma concentrations of Ile, Leu, Lys, Ala, Cys, and Pro linearly increased (P < 0.05). There was also an increase (linear, P < 0.05) in plasma concentrations of Ile, Leu, Met, Ala, Asp, Cys, and Pro as Val-FB was added to the diets, and the concentration of Val in plasma increased (linear and quadratic, P < 0.05). Using L-Val as the standard, the RBV of Val in Val-FB as determined by ADG, G:F, and final BW was 146%, 135%, and 143%, respectively, with 95% confidence intervals of 99% to 191%, 83% to 187%, and 70% to 217%, respectively. In conclusion, the linear regression estimated a RBV of at least 100% for Val in Val-FB relative to Val from L-Val, and pigs fed diet supplemented with Val-FB had greater final BW, ADG, and G:F than pigs fed diets supplemented with the same amount of Val from L-Val.

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 78-79
Author(s):  
Maryane S Faria de Oliveira ◽  
John K Htoo ◽  
Caroline J González-Vega ◽  
John E Thomson ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that Val from a Val-containing fermentation biomass product (Val-FB; 64.4% L-Val) has a bioavailability of 100% relative to L-Val (98% Val) when fed to weanling pigs. A Val-deficient basal diet containing 0.63% standardized ileal digestible (SID) Val was formulated. Six additional diets were prepared by supplementing the basal diet with 0.08, 0.16, or 0.24% L-Val or 0.12, 0.25, or 0.37% Val-FB to create experimental diets containing 0.71, 0.79, or 0.87% SID Val. A total of 224 weanling pigs (6.87 ± 0.64 kg) were allotted to a randomized complete block design with 7 diets and 8 replicate pens per diet. Diets were fed for 21 d and growth performance was measured on a pen basis. Orthogonal-polynomial contrasts were used to determine linear and quadratic effects of L-Val and Val-FB levels on performance and the effect of Val sources. A linear regression model based on performance was used to estimate the relative bioavailability (RBV) of Val in Val-FB relative to L-Val. The final body-weight (BW) and average-daily-gain (ADG) were greater (P < 0.01) for pigs fed diets supplemented with Val-FB than pigs fed diets supplemented with L-Val (Table 1). The average-daily-feed-intake (ADFI) decreased linearly (P < 0.01), whereas gain-to-feed ratio (G:F) increased (P < 0.01) by supplementing graded levels of both Val sources to the diets. The RBV of Val in Val-FB as determined by ADG, G:F, and final BW was 146, 135, and 143%, respectively, with 95% confidence intervals of 99 to 191%, 83 to 187%, and 70 to 217%, respectively. In conclusion, the linear regression estimated a RBV of at least 100% for Val-FB relative to L-Val, and pigs fed diets supplemented with Val-FB had greater ADG and final BW than pigs fed diets supplemented with the same amount of L-Val.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 61-61
Author(s):  
Chan Sol Park ◽  
Ayodeji S Aderibigbe ◽  
Gary Hayen ◽  
Olayiwola Adeola

Abstract Two experiments were conducted to evaluate the digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in dried yeast (DY) and soybean meal (SBM) fed to pigs. In Exp. 1, 30 barrows with an initial body weight (BW) of 20.7 ± 1.01 were assigned to 5 diets in a randomized complete block design with period and BW as blocking factors. A basal diet was prepared to contain corn, canola meal, and soybean oil as energy-contributing ingredients. Four additional diets were prepared by adding 5 or 10 g/kg DY or SBM at the expense of energy-contributing ingredients in the basal diet to estimate the DE and ME in test ingredients by regression analysis. On a dry matter basis, estimated DE and ME in DY were 4,022 and 3,352 kcal/kg, respectively, and those in SBM were 3,876 and 3,601kcal/kg, respectively. There was no difference in estimated DE or ME between DY and SBM. In Exp. 2, 21 barrows (initial BW = 20.0 ± 1.31 kg) surgically fitted with T-cannulas at the distal ileum were assigned to 3 diets in a randomized complete block design with BW as a blocking factor. Two diets were prepared to contain DY or SBM as the sole source of nitrogen, and a nitrogen-free diet was prepared to determine the basal ileal endogenous losses of AA. The SID of AA, except for Gly and Pro, in SBM were greater (P &lt; 0.05) than in DY. The SID of indispensable AA in DY ranged from 64.7% for Thr to 86.1% for Arg, whereas those in SBM ranged from 84.8% for Thr to 92.3% for Arg. In conclusion, energy values in DY was comparable with SBM, but the SID of most AA in DY were less than in SBM.


2013 ◽  
Vol 14 (2) ◽  
pp. 308-321
Author(s):  
Lina Maria Peñuela Sierra ◽  
Ivan Moreira ◽  
Antonio Claudio Furlan ◽  
Paulo Levi de Oliveira Carvalho ◽  
Ângela Rocio Poveda Parra ◽  
...  

Two experiments were carried out to determine the bioavailability of phosphorus in two spray-dried yeasts - sugarcane yeast (SCY), and sugarcane yeast + brewer's yeast (SCBY) - in starting pigs, by comparing different methods (Apparent Digestibility Coefficient of Phosphorus - ADCP; True Digestibility Coefficient of Phosphorus - TDCP; slope ratio; and standard curve). In experiment I, a digestibility assay were carried out using 30 cross breed pig with initial weigh of 22.69 ± 4.24kg, allotted in a completely randomized design. The mean ADCP and TDCP values were 62.68 and 64.15% for SCY and 77.01 and 79.33% for SCBY. ADCP and TDCP for SCBY were higher (P<0.05) than the values for SCY. In Experiment II, a growth test was conducted, 56 crossbred piglets, were utilized, with initial live weight of 15.11 ± 3.43kg, allotted in a completely randomized design, with seven treatments, four replications, and two pigs per experimental unit. The treatments consisted of a basal diet without supplementation with P and the same diet including supplementation with two levels of P (0.053% and 0.105%) from dicalcium phosphate, SCY and SCBY. The relative bioavailability mean value of 57.23% for SCY and 91.96% for SCBY, corresponding to 0.30% and 0.40% of available phosphorus, respectively.


Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan

The aim of this study was to assess the effects of dietary probiotic enhanced liquid acidifier combined with mannan-rich fraction on growth performance, nutrients digestibility in growing pigs. Thirty [(Duroc×Yorkshire)×Landrace] pigs with the average initial BW of 36.75±1.57 kg were allocated into three treatments by a randomized complete block design. There were five pens per treatment with six pigs per pen. Dietary treatments include: 1) CON (basal diet); 2) T1 (basal diet+probiotic 0.1%) and 3) T2 (basal diet+probiotic+mannan rich fraction 0.2%). the data were analyzed as a randomized complete block design using of SAS University Version 4.0. The model included the effects of block (replication) and treatment. Pen served as the experimental unit. During the entire experimental period of 6 weeks, results showed that addition of complex probiotic at the level of 0.2% to diet increased ADG significantly (p<0.05). Also, digestibility of DM and N tended to increase. To sum up, results in this experiment indicated that dietary [(probiotik×acidifier)×mannan-rich-fraction]  supplementation had a positive effect on growing pigs performance and nutrient digestibility


Author(s):  
Luca Lo Verso ◽  
Kristina Dumont ◽  
Martin Lessard ◽  
Karoline Lauzon ◽  
Chantale Provost ◽  
...  

Abstract This study aimed to evaluate the impact of grading levels of deoxynivalenol (DON) in the diet of weaned pigs, as well as the effects of a supplementation with antioxidants (AOX), hydrated sodium calcium aluminosilicates (HSCAS) and their combination on the growth, antioxidant status, immune and vaccine response against the porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus 2 (PCV2). At weaning, 336 piglets were allocated to six dietary treatments according to a randomized complete block design. Treatments were as follows: basal diet (CTRL); basal diet containing DON at 1.2 mg/kg (DON1.2); basal diet containing DON at 2.4 mg/kg (DON2.4); DON2.4 diet + a mix of AOX which included vitamins A and E at 20,000 IU and 200 IU/kg feed respectively, selenized yeast at 0.3 mg/kg and a grape seed extracts at 100 mg/kg feed (DON2.4+AOX); DON2.4 diet + modified HSCAS at 1 g/kg (DON2.4+HSCAS); DON2.4+AOX+HSCAS. Pigs were vaccinated against PRRSV and PCV2 at 7 d; at 0, 14 and 35 d growth performance were recorded, and blood samples were collected in order to evaluate the oxidative status, inflammatory blood markers, lymphocyte blastogenic response and vaccine antibody response. Increasing intake of DON resulted in a quadratic effect at 35 d in the lymphocyte proliferative response to Concanavalin A and PCV2 as well as in the anti-PRRSV antibody response, whereas the catalase activity decreased in DON2.4 pigs compared to the CTRL and DON1.2 groups (P ≤ 0.05). Compared to the DON2.4 diet, the AOX supplementation slightly reduced G:F ratio (P = 0.026) and increased the ferric reducing ability of plasma as well as α-tocopherol concentration (P &lt; 0.05), whereas the association AOX+HSCAS increased the anti-PRRSV IgG (P &lt; 0.05). Furthermore, the HSCAS supplement reduced haptoglobin levels in serum at 14 d compared to the DON2.4 group; however, its concentration decreased in all the experimental treatments from 14 d to 35 d and particularly in the DON2.4+AOX pigs, whereas a different trend was evidenced in the DON2.4+HSCAS group, where over the same period haptoglobin concentration increased (P &lt; 0.05). Overall, our results show that the addition of AOX and HSCAS in the diet may alleviate the negative effects due to DON contamination on the antioxidant status and immune response of vaccinated weanling pigs.


2015 ◽  
Vol 45 (6) ◽  
pp. 1093-1098 ◽  
Author(s):  
Marconi Bonfim de Santana ◽  
Antonio Diego Brandão Melo ◽  
Daniel Ribeiro Cruz ◽  
Cesar Augusto Pospissil Garbossa ◽  
Carla de Andrade ◽  
...  

An experiment was conducted to evaluate the addition of sodium butyrate, plant extracts and nucleotides on weanling pig performance, digestive content pH, organ morphometry, and intestinal epithelial histology. A total of 90 piglets at 21 days of age and an average initial weight of 6.35±0.34kg were used. The piglets were distributed in a randomized complete block design with five treatments, six replicates, and three animals per experimental unit. The treatments consisted of the following: Control: basal diet without antibiotic; Antibiotic: basal diet with 40mg kg-1 colistin sulfate and Additive: 1000, 1500 and 2000mg kg-1 of a combination of sodium butyrate + plant extracts + nucleotides. The experiment lasted 35 days, at which time one animal was slaughtered to assess pH of the digestive contents, morphometry of the organs and histology of the intestinal epithelium. No differences were found (P>0.05) in the performance, pH of the digestive contents, morphometry of the organs or histology of the intestinal epithelium by the analysis of orthogonal polynomials or contrasts. The combination of sodium butyrate, plant extracts and nucleotides not improved the productive characteristics of weanling pigs.


2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Tewodros Alemu ◽  
Alemu Dagnachew ◽  
Alemu Tsegaye

Abstract The experiment was conducted using 36 intact yearling males of Abergelle breed and Abergelle cross bred goats (50%) with initial live weight of 18.92 ± 0.72 kg (mean ± SE). The objective of the experiment was to evaluate the effect of concentrate supplementation on carcass parameters and meat sensory quality of genotypes. Goat genotypes were blocked based on initial body weight and were randomly assigned to dietary treatments. The experimental design was 2 by 3 factorial in randomized complete block design. The treatments included local grass hay as basal diet and supplementation with concentrate (184, 368, and 552 g/d on DM basis). Effects of genotype and diet were significant on the main carcass parameters (P &lt; 0.05) but genotype did not show effect on edible offal components (P &gt; 0.05). Diet had a significance effect on meat flavor (P &lt; 0.05) but not on tenderness, juiciness, and soup flavor (P &gt; 0.05). Genotype had no effect (P &gt; 0.05) on all sensory attributes. Goats feeding on higher level of concentrate had heavier total edible offal components (P &lt; 0.05) than feeding on lower level of concentrates but not difference between genotypes (P &gt; 0.05). The cross breed goats feeding on higher level of concentrate showed higher percentage of nonedible offal (P &lt; 0.01) particularly gut content, foreleg, and hind leg than pure breed and lower level of concentrate. The digestibility and chemical composition of meat of the genotypes were not addressed in the experiment and hence need to be studied further.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 182-183
Author(s):  
Joe D Crenshaw ◽  
Joy M Campbell ◽  
Javier Polo

Abstract The objective was to determine if a 3-phase feed program (CON) without spray-dried bovine plasma (SDP) using a budgeted amount of phase 1 diet (2.3 kg/pig), followed by phase 2 diet offered to d 21 post-weaning results in similar pig performance and economics compared to a 3-phase program (SDP) using 5% SDP in phase 1 diet provided d 0-14, 1.25% SDP in phase 2 diet offered d 14-21 post-weaning, with both programs using a common phase 3 diet without SDP offered d 21-48 post-weaning. Female weaned pigs (18–21 d of age; 5.02 ± 0.07 kg initial BW) were randomly allotted by BW to pens in 2 rooms to provide 23–24 pigs/pen (666 total pigs) and 14 pens/feed program. Pen data was analyzed using a randomized complete block design for effects of room and feed program using the covariance of initial BW. By d 14 pigs fed program SDP had higher (P < 0.01) ADG (159 vs 111 g/d), GF (0.85 vs 0.54), BW (7.3 vs 6.6 kg) and tended (P = 0.06) to have lower ADFI (191 vs 205 g/d) compared to CON program. By d 48 pigs fed SDP program had higher (P < 0.05) final BW (25.2 vs 24.2 kg), GF (0.60 vs 0.57) and tended (P = 0.09) to have higher ADG than CON program. Pig mortality and culls did not differ (P > 0.10) between feed programs. Assuming $4.40 kg BW, income and feed cost/pig was higher (P < 0.05) for SDP vs CON program with a value over cost advantage (P = 0.12) of $2.95/pig fed SDP. In conclusion lower cost feed programs without SDP can result in less income due to reduced final BW and GF by the end of the nursery.


Sign in / Sign up

Export Citation Format

Share Document