scholarly journals A Microsatellite Multiplex Assay for Profiling Pig DNA in Mosquito Bloodmeals

2019 ◽  
Vol 56 (4) ◽  
pp. 907-914 ◽  
Author(s):  
John B Keven ◽  
Edward D Walker ◽  
Patrick J Venta
Keyword(s):  
Population Analysis ◽  
Allelic Diversity ◽  
Fluorescent Labeling ◽  
Specific Marker ◽  
Genetic Profiling ◽  
Nuclear Families ◽  
High Allelic Diversity ◽  
And Gender ◽  
Microsatellite Multiplex ◽  
The Individual

Abstract Genetic profiling has been used to link mosquito bloodmeals to the individual humans, but this analysis has not been done for other mammalian bloodmeals. In this study, we describe a microsatellite-based method for identifying individual pigs in mosquito bloodmeals based on their unique multilocus genotypes. Eleven tetranucleotide microsatellites and a sex-specific marker were selected based on Smith-Waterman DNA sequence alignment scores from the reference genome and primers were designed with features that reduce primer dimers, promote complete adenylation, and enable fluorescent labeling of amplicons. A multiplex polymerase chain reaction (PCR) assay was optimized and validated by analyzing DNA of individual pigs from several nuclear families and breeds before it was used to analyze genomic DNA of pig-derived mosquito bloodmeals from villages of Papua New Guinea. Population analysis of the nuclear families showed high expected and observed heterozygosity. The probability of observing two unrelated or sibling individuals sharing the same genotype at a single microsatellite locus or a combination of loci was vanishingly low. Samples had unique genotypes and gender was accurately predicted. Analysis of 129 pig bloodmeals identified 19 unique genotypes, which varied greatly in frequency in the mosquito bloodmeal samples. The high allelic diversity of the microsatellite loci and low probability of false attribution of identity show that this genotyping method reliably distinguishes distantly and closely related pigs and can be used to identify individual pigs from genotyped mosquito bloodmeals.

scholarly journals Unexpectedly High Allelic Diversity at the KIT Locus Causing Dominant White Color in the Domestic Pig

Genetics ◽  
2002 ◽  
Vol 160 (1) ◽  
pp. 305-311
Author(s):  
G Pielberg ◽  
C Olsson ◽  
A-C Syvänen ◽  
L Andersson
Keyword(s):  
Coat Color ◽  
Allelic Diversity ◽  
Growth Factor Receptor ◽  
Small Sample ◽  
Color Variation ◽  
Large White ◽  
High Genetic Diversity ◽  
High Allelic Diversity ◽  
Stem Cell Growth Factor ◽  
New Alleles

Abstract Mutations in KIT encoding the mast/stem cell growth factor receptor (MGF) are responsible for coat color variation in domestic pigs. The dominant white phenotype is caused by two mutations, a gene duplication and a splice mutation in one of the copies leading to skipping of exon 17. Here we applied minisequencing and pyrosequencing for quantitative analysis of the number of copies with the splice form. An unexpectedly high genetic diversity was revealed in white pigs. We found four different KIT alleles in a small sample of eight Large White females used as founder animals in a wild boar intercross. A similar number of KIT alleles was found in commercial populations of white Landrace and Large White pigs. We provide evidence for at least two new KIT alleles in pigs, both with a triplication of the gene. The results imply that KIT alleles with the duplication are genetically unstable and new alleles are most likely generated by unequal crossing over. This study provides an improved method for genotyping the complicated Dominant white/KIT locus in pigs. The results also suggest that some alleles may be associated with negative pleiotropic effects on other traits.

Entrepreneurship in regions: differentiated impacts of the socio cultural and gender types

2015 ◽  
Vol 28 (1) ◽  
pp. 45-76 ◽  
Author(s):  
Claudio Mancilla ◽  
José Ernesto Amorós
Keyword(s):  
Negative Impact ◽  
Positive Impact ◽  
Fear Of Failure ◽  
Content Type ◽  
Reference Models ◽  
Cultural Aspects ◽  
And Gender ◽  
The Individual ◽  
Practical Implications ◽  
Secondary City

Purpose The purpose of this paper is to study the differentiated impact of factors that influence the propensity to entrepreneur in a sample of people in Chile. A distinction is made between individuals that live in primary cities and secondary cities. The differentiating factors are socio‐cultural aspects (reference models – positive examples of entrepreneurs – and perception of social fear of failure) and the gender of the individual. Design/methodology/approach For the research data from the survey used in Chile by the Global Entrepreneurship Monitor for the years 2010 and 2011 were used. A logit model was used to determine the differentiated impact of the analysed factors and interactions were done using the method proposed by Corneliâen and Sonderhof (2009). Findings These showed that the fact that an individual lives in a secondary city decreases his entrepreneurship probability. The positive impact that the reference models have is weaker in women. Contrary to what was expected, the negative impact of the fear of failure perception is weaker in women. Practical implications These results have the implications to suggest focused public policies and differentiations that consider the socio‐cultural, territorial (focused in cities) and gender aspects. Originality/value The research contributes by giving empirical evidence of the existence of the negative impact of living in a secondary city and of differentiated effects of socio‐cultural factors from the gender perspective.

scholarly journals ‘'Sex Changes'? Paradigm Shifts in ‘Sex’ and ‘Gender’ following the Gender Recognition Act?’

2007 ◽  
Vol 12 (1) ◽  
pp. 75-89 ◽  
Author(s):  
Stephen Whittle ◽  
Lewis Turner
Keyword(s):  
Sex Change ◽  
Gender Recognition ◽  
Sex And Gender ◽  
Gender Variance ◽  
Paradigm Shifts ◽  
History Of ◽  
And Gender ◽  
The Individual ◽  
The Uk ◽  
Legal Understanding

Gender transformations are normatively understood as somatic, based on surgical reassignment, where the sexed body is aligned with the gender identity of the individual through genital surgery – hence the common lexicon ‘sex change surgery’. We suggest that the UK Gender Recognition Act 2004 challenges what constitutes a ‘sex change’ through the Act's definitions and also the conditions within which legal ‘recognition’ is permitted. The sex/gender distinction, (where sex normatively refers to the sexed body, and gender, to social identity) is demobilised both literally and legally. This paper discusses the history of medico-socio-legal definitions of sex have been developed through decision making processes when courts have been faced with people with gender variance and, in particular, the implications of the Gender Recognition Act for our contemporary legal understanding of sex. We ask, and attempt to answer, has ‘sex’ changed?

The Future Profile of Health Promotion and Disease Prevention in Japan

2005 ◽  
Vol 44 (02) ◽  
pp. 342-347 ◽  
Author(s):  
N. Doba ◽  
S. Hinohara
Keyword(s):  
Cohort Study ◽  
Population Analysis ◽  
Well Being ◽  
The Elderly ◽  
Health Evaluation ◽  
Social Emotional ◽  
Study Program ◽  
Healthy Elderly ◽  
The Individual ◽  
Apparently Healthy

Summary Objectives: One of the serious challenges for Japanese healthcare is the aging population. Analysis of health evaluation data, especially of the elderly over 75 years, is considered very important. In view of this prolonged life expectancy in Japan, our government started the new campaign of Healthy Japan 21, of which details are described, and also we will demonstrate the general profile of our perspective cohort study program concerning the new elder citizens in Japan. Methods and Results: Our group has started a health evaluation program for those apparently healthy new elder citizens over the age of 75. A ten-year cohort study is in progress, which is designed to accumulate health check-up data annually. The study collects information on physical well being, as well as information on the individual’s lifestyle, and social, emotional and spiritual environment. Such health appraisal is of primary importance. Preliminary results will be demonstrated with their special implications in clinical and epidemiological significance. The degree of frailty of the individual was also carefully evaluated, so that we can learn about the QOL of the seniors in Japan. Conclusions: At the present stage, we can only report a part of our study including the mental and spiritual environments of the individual. This kind of outcome study will give us some insights concerning the natural progress of frailty in the apparently healthy elderly.

scholarly journals CNV Neurons Are Rare in Aged Human Neocortex

2018 ◽  
Author(s):  
William D. Chronister ◽  
Margaret B. Wierman ◽  
Ian E. Burbulis ◽  
Matthew J. Wolpert ◽  
Mark F. Haakenson ◽  
...  
Keyword(s):  
Copy Number Variants ◽  
Allelic Diversity ◽  
Population Level ◽  
Information Criterion ◽  
Published Data ◽  
Tissue Quality ◽  
Cell Library ◽  
Detection Approach ◽  
Human Neurons ◽  
The Individual

AbstractMegabase-scale somatic copy number variants (CNVs) alter allelic diversity in a subset of human neocortical neurons. Reported frequencies of CNV neurons range from ∼5% of neurons in some individuals to greater than 30% in other individuals. Genome-wide and familial studies implicitly assume a constant brain genome when assessing the genetic risk architecture of neurological disease, thus it is critical to determine whether divergent reports of CNV neuron frequency reflect normal individual variation or technical differences between approaches. We generated a new dataset of over 800 human neurons from 5 neurotypical individuals and developed a computational approach that measures single cell library quality based on Bayesian Information Criterion and identifies integer-like variant segments from population-level statistics. A brain CNV atlas was assembled using our new dataset and published data from 10 additional neurotypical individuals. This atlas reveals that the frequency of neocortical CNV neurons varies widely among individuals, but that this variability is not readily accounted for by tissue quality or CNV detection approach. Rather, the age of the individual is anti-correlated with CNV neuron frequency. Fewer CNV neurons are observed in aged individuals than young individuals.

scholarly journals THE RELATIONSHIP BETWEEN GENDER ROLE ATTITUDES AND EPISTEMOLOGICAL BELIEFS OF UNIVERSITY STUDENTS

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Alper Çuhadaroğlu
Keyword(s):  
Gender Roles ◽  
University Students ◽  
Gender Role ◽  
Epistemological Beliefs ◽  
Qualitative Data ◽  
Gender Role Attitudes ◽  
Study Group ◽  
And Gender ◽  
The Individual ◽  
The Relationship

In this study, the relationships between university students and their perceptions of gender roles and epistemological beliefs were investigated. Gender roles are a phenomenon that are determined by culture, and begin to emerge at an early age, which may include some stereotypical behaviors along with a number of attitudes, duties and obligations that the individual is expected to perform as a woman or a man. Epistemological belief is seen as an individual feature of how knowing and learning take place. In this study, a mixed method was used. The quantitative study group consists of 517 students from both universities, while the qualitative study group consists of 85 people. Gender Role Attitudes Scale and Epistemological Beliefs Scale were used to collect quantitative data. In order to obtain qualitative data, participants were given a form consisting of open-ended questions. According to the analyses, it was determined that there was a significant relationship between the participants' epistemological beliefs and gender roles attitudes and, epistemological beliefs were a significant predictor of gender roles attitudes. The results obtained are discussed in line with the existing literature. <p> </p><p><strong> Article visualizations:</strong></p><p><img src="/-counters-/edu_01/0798/a.php" alt="Hit counter" /></p>

scholarly journals Listening to Local and Foreign Sex Buyers of Men and Women in Cambodia

2016 ◽  
Vol 3 (1) ◽  
pp. 56-79
Author(s):  
Samantha Sommer Miller ◽  
Glenn Miles ◽  
James Havey
Keyword(s):  
Sex Workers ◽  
Sex Industry ◽  
Sex And Gender ◽  
Phnom Penh ◽  
Bisexual Males ◽  
And Bisexual ◽  
And Gender ◽  
Sex With Men ◽  
The Individual ◽  
Sexual Services

Research on prostitution and trafficking has largely focused on the exploitation of girls and young women. This research comes out of the “Listening to the Demand” two-part study by an independent research team on the sex industry in Phnom Penh, Cambodia. “Listening to the Demand” is a series of research exploring often over-looked populations in the anti-trafficking conversation, including men and transgender people. The first of the studies was completed in 2013 and focuses on men who purchased sex with female sex workers. Interviews of 50 Cambodian and 50 foreign heterosexual and bisexual males explored the respondents’ views and use of prostituted women in Southeast Asia’s sex industry. The second part of the research was completed in 2014 and focuses on men who purchase sex with men. In this second part of the project, 51 Cambodian and 23 foreign men who have sex with men were interviewed about their views of prostitution, the individual sex worker, and their experiences of Cambodia’s sex industry. Due to its comparative nature, the research seeks to deliver information on the differences in culture between the foreign and Cambodian men who seek to pay for sexual services. Results point to the need for proper sex and gender education as well as different approaches when planning projects to reach out to men purchasing sex. In gaining a deeper knowledge of the beliefs and behaviours among the demand population, the findings suggest more holistic approaches are needed to combat the exploitation of sexual services in Cambodia.


scholarly journals A multidimensional model of healthy ageing: proposal and evaluation of determinants based on a population survey in Ecuador

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
María Fernanda Rivadeneira ◽  
María José Mendieta ◽  
Jessica Villavicencio ◽  
José Caicedo-Gallardo ◽  
Patricio Buendía
Keyword(s):  
Older Adults ◽  
Gender Equity ◽  
Well Being ◽  
Healthy Ageing ◽  
World Health ◽  
Multidimensional Model ◽  
Cross Sectional ◽  
Intrinsic Capacity ◽  
And Gender ◽  
The Individual

Abstract Background Healthy ageing is a complex construct which involves multiple dimensions. Previous studies of healthy ageing have focused only on measuring the intrinsic capacity of the older person. The objectives of this study were to design a multidimensional model of healthy ageing and to identify its determinants from national data in Ecuador. Methods A cross-sectional analytical study was carried out from the National Survey of Health and Well-being of the Older Adult, 2010. Sample was 1797 adults aged 65 years or more. A multidimensional model was designed based on the World Health Organization’s concept of healthy ageing. For the analysis, two groups were created: a healthy ageing and a less healthy ageing group. Bivariate and multivariate logistic regressions were performed to analyze the probability of belonging to the healthy group according to sex, age, area of ​​residence, level of education, perceived health status, perceived life satisfaction, and poverty by income level. Results The 53.15% of the sample was classified in the healthy ageing group. Women and the poorest older adults were less likely to be in the healthy ageing group (OR 0.58; 95% CI 0.464–0.737; OR 0.44; 95% CI 0.343–0.564). Older adults with secondary education or higher, who considered their health as excellent and who were satisfied with their life, had a greater probability of being in healthy ageing group (OR 2.61; 95% CI 1.586–4.309; OR 28.49; 95% CI 3.623–224.02; OR 0.23; 95% CI 0.165–0.341). Conclusions This study contributes with a multidimensional approach to healthy ageing. It proposes to evaluate the intrinsic capacity of the individual, the social and political environment and the interaction with it, through indicators that discriminate who are ageing in a healthy way and who are not. By using this model, it was identified that gender and economic situation seem to play an important role on heathy ageing of the Ecuadorian population. Public policies are necessary to promote healthy ageing, especially focused on improving socioeconomic conditions and gender equity.

scholarly journals Theorizing Cultural and Social Intersections of Age and Gender: The Approach of Anocriticism

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 826-826
Author(s):  
Roberta Maierhofer
Keyword(s):  
Cultural Construction ◽  
Cultural Representations ◽  
Four Dimensions ◽  
Gerontological Research ◽  
Gender And Age ◽  
Methodological Approaches ◽  
And Gender ◽  
Narrative Performance ◽  
The Individual ◽  
Individual Dimension

Abstract This contribution discusses empirical applications of the approach of ‘anocriticism’ in interdisciplinary gerontological research. Despite the connection in terms of epistemology and ontology, the intersection of gender and age has been mostly ignored, privileging works focusing either on age or gender (Calasanti & Slevin 2001:27; Denninger & Schütze 2017:7). Age/ing Studies, however, would not have been established as a field without the theoretical and methodological approaches of feminist theory (Maierhofer (2019:2). Anocriticism was originally developed in order to investigate cultural representations of age/ing (Maierhofer 2003, 2004b, 2004a, 2007, 2012), but has recently been taken up in social sciences (Ratzenböck 2016a, 2016b, 2017a, 2017b; Gales and Loos 2020, forthcoming) in order to draw attention to four dimensions: (a) age and aging’s collective cultural construction and relation to gender, (b) the individual dimension of aging, (c) people’s interpretative power and narrative performance, and (d) age/ing’s potential for resistance and change.

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