First Report on the Use of Larvae of Cochliomyia macellaria (Diptera: Calliphoridae) for Wound Treatment in Veterinary Practice

2019 ◽  
Vol 57 (3) ◽  
pp. 965-968 ◽  
Author(s):  
F S Masiero ◽  
E S V Aguiar ◽  
D I B Pereira ◽  
P J Thyssen
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Complete Healing ◽  
Wound Healing Process ◽  
Wound Treatment ◽  
Treatment Alternative ◽  
Qualitative And Quantitative ◽  
Cochliomyia Macellaria ◽  
Complex Wound ◽  
Points Of View

Abstract Factors contributing to the delay or prevention of the natural wound healing process include infection and ineffective conventional treatment. Alternative therapies, such as the maggot debridement therapy (MDT), may be helpful for successful treatment in these cases. Aiming to disseminate information about the possibility of using other species of flies for wound treatment, besides the best known Lucilia sericata Meigen, 1826 (Diptera, Calliphoridae), we report here a successful MDT case with the application of Cochliomyia macellaria (Fabricius, 1775) larvae to treat an infected wound with extensive area of necrotic tissue in a dog. Five sterile larvae were applied to each square-cm of lesion and kept on the animal for only 48 h. The healing was successful, from both qualitative and quantitative points of view. The ratio of wound healing (RWH) reached almost 50% on the 5th day and 100% on the 14th day after MDT. Although the overall animal prognosis had been unfavorable, mainly due to the sepsis, the patient began to recover and had improved clinical condition from the fifth day after MDT. This study shows the importance and effectiveness of MDT in promoting faster and more complete healing of a complex wound.

scholarly journals Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness

2021 ◽  
Vol 12 ◽  
Author(s):  
Laryssa C. Manigat ◽  
Mitchell E. Granade ◽  
Suchet Taori ◽  
Charlotte Anne Miller ◽  
Luke R. Vass ◽  
...  
Keyword(s):  
Wound Healing ◽  
T Cell ◽  
Healing Process ◽  
Cell Activity ◽  
Regulatory Function ◽  
Burned Area ◽  
Wound Healing Process ◽  
Wound Model ◽  
Complex Wound

The diacylglycerol kinases (DGKs) are a family of enzymes responsible for the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). In addition to their primary function in lipid metabolism, DGKs have recently been identified as potential therapeutic targets in multiple cancers, including glioblastoma (GBM) and melanoma. Aside from its tumorigenic properties, DGKα is also a known promoter of T-cell anergy, supporting a role as a recently-recognized T cell checkpoint. In fact, the only significant phenotype previously observed in Dgka knockout (KO) mice is the enhancement of T-cell activity. Herein we reveal a novel, macrophage-specific, immune-regulatory function of DGKα. In bone marrow-derived macrophages (BMDMs) cultured from wild-type (WT) and KO mice, we observed increased responsiveness of KO macrophages to diverse stimuli that yield different phenotypes, including LPS, IL-4, and the chemoattractant MCP-1. Knockdown (KD) of Dgka in a murine macrophage cell line resulted in similar increased responsiveness. Demonstrating in vivo relevance, we observed significantly smaller wounds in Dgka-/- mice with full-thickness cutaneous burns, a complex wound healing process in which macrophages play a key role. The burned area also demonstrated increased numbers of macrophages. In a cortical stab wound model, Dgka-/- brains show increased Iba1+ cell numbers at the needle track versus that in WT brains. Taken together, these findings identify a novel immune-regulatory checkpoint function of DGKα in macrophages with potential implications for wound healing, cancer therapy, and other settings.

scholarly journals Investigation of Wound Healing Potential of Azadirachta indica Seed Extract using Wistar Rats

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
I. A. Ajayi ◽  
O. B. Omolere
Keyword(s):  
Wound Healing ◽  
Azadirachta Indica ◽  
Wistar Rats ◽  
Wound Closure ◽  
Healing Process ◽  
Wound Healing Process ◽  
Wound Treatment ◽  
Phytochemical Screening ◽  
Wound Area ◽  
Seed Extracts

This study investigated the wound healing potential of hexane and methanolic seed extracts of Azadirachta indica using 35 wistar rats that were divided into 5 groups of 7 rats each. Phytochemical screening and antimicrobial activity of the extracts were carried out while the wound healing potential was evaluated by treating the test rats with 5 % and 10 % hexane and methanol extracts in an experiment that lasted for 21 days. Wound area and percentage of wound closure of the rats were noted at four-day intervals and at 21 days, the blood and organs of the rats were subjected to haematological and histopathological analyses respectively. The extracts were found to contain tannins, glycosides and phenols and they inhibited the growth of tested organisms. All the test rats displayed better and faster healing than the control ones but there were no significant differences between their haematological and histophatological results. The seed extracts quickened the wound healing process of the rats and might therefore be useful in wound treatment.

Recent Developments in Nano-Drug Delivery Systems Loaded by Phytochemicals for Wound Healing

2020 ◽  
Vol 20 (18) ◽  
pp. 1867-1878
Author(s):  
Neda Mirrezaei ◽  
Rezvan Yazdian-Robati ◽  
Fatemeh Oroojalian ◽  
Amirhossein Sahebkar ◽  
Maryam Hashemi
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Antibacterial Activities ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Wound Treatment ◽  
Therapeutic Modalities ◽  
Multi Stage ◽  
Recent Developments ◽  
Applicable Knowledge

Wound healing is a multi-stage process during which a cascade of molecular and cellular events collaborate to restore the damaged tissue to its healthy state. The inability of the available therapies to effectively heal the wounds has imposed major problems on healthcare systems. Therefore, developing novel therapeutic modalities capable of enhancing wound healing process with no/or limited scar formation is of more importance. Different studies have investigated the potential of phytochemicals on the wound healing process. They have shown to exert anti-inflammatory, antioxidant, and antibacterial activities as well as promoting collagen synthesis and deposition, leading to enhancing wound healing. Nanotechnology, as an applicable knowledge, has provided versatile means to improve the efficiency and effectiveness of wound treatment. The application of nanoparticles has conferred various advantages in the field of wound treatment. They protect the therapeutics from degradation, release the cargo in a controlled fashion, possess healing properties, and can act as extracellular matrix (ECM) mimic. In this review, we discuss the naturally-occurring compounds with wound healing properties and their nano-formulation for skin wound therapy.

scholarly journals The In Vivo, In Vitro and In Ovo Evaluation of Quantum Dots in Wound Healing: A Review

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 191
Author(s):  
Atiqah Salleh ◽  
Mh Busra Fauzi
Keyword(s):  
Quantum Dots ◽  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Wound Healing Process ◽  
Cellular Interactions ◽  
In Ovo ◽  
Complex Wound

Wound is defined as primarily damaged or disruption of skin contributed to the loss of its microstructure stability and which undergoes complex wound healing process. However, there are tons of factors that could affect the wound healing process such as infection and slow angiogenesis. Involvement of nanotechnologies therapies in wound care research aims to facilitates this healing process. Quantum dots (QDs) are an advanced nanomaterial technology found to be useful in clinical and biomedical applications. This review has been carried out to provide a summary of the application of QDs in acute or chronic wound healing. A thorough searching was done via Web of Science and SCOPUS database to obtain relevant articles including the in vivo, in vitro and in ovo studies. The results demonstrated a similar effect of different types of QDs, or an improvement of QDs in wound healing, antibacterial and angiogenesis properties. This review demonstrated the effectiveness of QDs for the wound healing process mainly by their antibacterial activity. Uniquely, the antibacterial effect unraveled an increasing trend over time influenced by the various concentration of QDs. In conclusion, the application of QDs support the wound healing phases and proven to be effective in vivo, in vitro and in ovo. However, the future QDs work should focus on the molecular level for the details of cellular interactions and pathways.

scholarly journals Honey in wound healing: An updated review

2021 ◽  
Vol 16 (1) ◽  
pp. 1091-1100
Author(s):  
Hanaa Tashkandi
Keyword(s):  
Wound Healing ◽  
Antimicrobial Agents ◽  
Chronic Wounds ◽  
Healing Process ◽  
Trauma Surgery ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Wound Healing Process ◽  
Wound Treatment ◽  
Application Procedure

Abstract Wound healing is a complex process with many interdependent pathophysiological and immunological mediators to restore the cellular integrity of damaged tissue. Cutaneous wound healing is the repair response to a multitude of pathologies induced by trauma, surgery, and burn leading to the restoration and functionality of the compromised cells. Many different methods have been employed to treat acute and chronic wounds, such as antimicrobial therapy, as most wounds are susceptible to infection from microbes and are difficult to treat. However, many antimicrobial agents have become ineffective in wound treatment due to the emergence of multiple drug-resistant bacteria, and failures in current wound treatment methods have been widely reported. For this reason, alternative therapies have been sought, one of which is the use of honey as a wound treatment agent. The use of honey has recently gained clinical popularity for possible use in wound treatment and regenerative medicine. With this high demand, a better delivery and application procedure is required, as well as research aiming at its bioactivity. Honey is a safe natural substance, effective in the inhibition of bacterial growth and the treatment of a broad range of wound types, including burns, scratches, diabetic boils (Skin abscesses associated with diabetic), malignancies, leprosy, fistulas, leg ulcers, traumatic boils, cervical and varicose ulcers, amputation, burst abdominal wounds, septic and surgical wounds, cracked nipples, and wounds in the abdominal wall. Honey comprises a wide variety of active compounds, including flavonoids, phenolic acid, organic acids, enzymes, and vitamins, that may act to improve the wound healing process. Tissue-engineered scaffolds have recently attracted a great deal of attention, and various scaffold fabrication techniques are being researched. Some incorporate honey to improve their delivery during wound treatment. Hence, the aim of this review is to summarize recent studies on the wound healing properties of honey.

Effect of Trishothadi Lepam on Tropical foot ulcer – Case Report

2020 ◽  
Vol 8 (05) ◽  
Author(s):  
Jayshree Satpute ◽  
Rajesh Gundre ◽  
Chandrakant Chate
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Foot Ulcer ◽  
Cost Effective ◽  
Underlying Mechanism ◽  
Complete Healing ◽  
Wound Healing Process ◽  
Ulcer Case ◽  
Group A ◽  
Group B

Siravyadhana and Trishothadi Lepam application was intended for healthy wound healing. Probably it will have an understanding of underlying mechanism of relieving vascular stasis, reducing venous congestion and beginning of re-vascularization and other wound healing process. The Group-B showing maximum percentage of benefit followed by Group-A. Hence it can be concluded that overall benefit is found maximum in Group-A where Siravyadhana with Trishothadi Lepam was administered. The  drug  Trishothadi Lepam might be shown the efficacy due to Lekhana, Kledahara, Shoshana, Shotha & Dahahara, RaktaShodhaka and Twaka Prasadana properties. this procedure is found to be very much safe, simple, cost-effective, easily administered in the patients of foot ulcers leading them for complete healing avoiding the most unwanted outcome of amputation.

scholarly journals EFFECT MOIST WOUND HEALING TECHNIQUE TOWARD DIABETES MELLITUS PATIENTS WITH ULKUS DIABETIKUM IN DHOHO ROOM RSUD PROF Dr. SOEKANDAR MOJOSARI

2017 ◽  
Vol 6 (1) ◽  
pp. 63-69
Author(s):  
Lutfi Wahyuni
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Research Design ◽  
Cell Damage ◽  
Healing Process ◽  
Wound Healing Process ◽  
Diabetic Patients ◽  
Treatment Technique ◽  
Wound Treatment ◽  
Granulation Process

Wound healing of ulkus diabetikum depends on wound treatment given; an appropriate wound treatment technique is able to help the healing process quickly and prevent amputation effectively. It needs wound treatment that is able to prevent advanced complication and increase wound healing process such as moist wound healing. This study aimed to aplicated technique with moist wound healing toward diabetes mellitus patients with ulcer diabetic in dhoho room RSUD Prof Dr. Soekandar Mojosari. Deskriptive explorative research design was used in this study. The number of sample were 20 respondents who were the whole ulcer diabetic patients on January – March 2016. The date were collected from the 1st and 7th wound treatment days of embroider medic and used observation sheets. The findings revealed that 20 respondents were on wound healing degree with regeneration wound; it means that the whole respondents cared by moist wound healing within 7 days got regeneration wound or net amelioration. Moist wound healing technique is able to keep moist of wound itself, so that it can facilitate cell and wound movement as well as expedite the granulation process about 50%. Therefore, after doing wound healing during 7 days, the significant change was on wound granulation development. Besides, it can minimize cell damage while the alteration bandage in order to prevent the new trauma and wound spread.

scholarly journals Phytochemical Screening and Wound Healing Activity of Different Leaf Extracts of Rhynchosia rothii in Rats

2021 ◽  
pp. 386-393
Author(s):  
Sharad D. Tayade ◽  
Narendra Silawat
Keyword(s):  
Wound Healing ◽  
Body Weight ◽  
Healing Process ◽  
Complete Healing ◽  
Wound Contraction ◽  
Wound Healing Process ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Healing Property ◽  
Wound Healing Activity

Aims: The aim of the present work to perform phytochemical screening and wound healing activity of different leaf extract of Rhynchosia rothii. Place and Duration of the Study: The present work has been carried out at Oriental University, Indore between the duration of November-2020 to January-2021. Methodology: Rhynchosia rothii dried leaf powder was subjected to continuous extraction with a soxhlet extractor using various organic solvents. Preliminary phytochemical screening with various qualitative chemical tests revealed the presence of Alkaloids, Tannins, Flavonoids, proteins, and mucilage present in Rhynchosia rothii leaf extracts. Wister rats of both sexes weighing 150-200 g were used for experimental purposes. The animals were kept in polypropylene cages at room temperature and with a light/dark cycle of 12:12 hours.  All the test extracts at a different dose level and standard were administered topically once daily from day 0 to the day of complete healing or the 21st postoperative day, whichever occurred earlier. Results: A significant increase in wound healing activity was observed in animals treated with alcoholic and aqueous extracts of Rhynchosia rothii leaves at a dose of 200 mg/kg body weight, compared to control treatments. Both methanolic and standard extracts of Rhynchosia rothii (200 mg/kg body weight) showed a dose-dependent increase in the rate of wound contraction. The methanolic extract of Rhynchosia rothii at a dose of 200 mg/kg body weight showed an increase in the percentage of wound contraction from 40.08% to 88.90% from day 3 to 12. The aqueous (standard) extract of Rhynchosia rothii at a dose of 200 mg/kg body weight showed an increase in the percentage of wound contraction from 46.02% to 100% from day 3 to 12. Conclusion: From the previous results it was concluded that the Wound represents a serious health problem, both in terms of morbidity and mortality. Wound healing is a critical response to tissue integrity. Therefore, the wound healing property of Rhynchosia rothii can be attributed to the phytoconstituents present in it, which may be due to its individual or additive effect that accelerates the wound healing process. Since Rhynchosia rothii is grown in abundance in India and rest of other part of world, it could be a fairly good and easily available therapeutic agent due to its naturally occurring tendency for healing wounds as a healer, as well as controlling abnormal healing.

scholarly journals Evaluation of Topicure® Plus spray on sheep infested with Myiasis

2017 ◽  
Vol 3 (2) ◽  
pp. 216-220
Author(s):  
Ramasamy Selvam ◽  
Subramaniyam Suresh ◽  
D’Souza Prashanth
Keyword(s):  
Wound Healing ◽  
Body Weight ◽  
Granulation Tissue ◽  
Healing Process ◽  
Local Treatment ◽  
Complete Healing ◽  
Activity Level ◽  
Repellent Activity ◽  
Wound Healing Process ◽  
Repellent Property

The present study was designed to evaluate the therapeutic efficacy of polyherbal aerosol spray - Topicure® Plus against maggot infested wounds in sheep. Two sheep presented at Vellore Veterinary Dispensary with a history of maggot infested wound were selected. Based on the severity of wound, antibiotics (Ampicillin - 10 mg/kg body weight) and NSAIDs (Meloxicam - 0.5 mg/kg body weight on 1st day) were administered. The site of wound was clipped off the hair and cleaned with sterile saline solution and wiped with dry sterile cotton on first day of the treatment. Then Topicure® Plus was sprayed in sufficient quantities to cover the entire wound twice daily till complete healing of wound occurs. When Topicure® Plus spray was administered concurrent local treatment with other fly repellents/maggoticidal agents was not followed. Wound was observed for the changes in swelling, discharge, granulation tissue and wound healing process following the treatment till complete recovery. Similarly, fly repellent activity and duration, maggot repellent property, activity level and product satisfaction score were also recorded. The results indicate that Topicure® Plus had ensured faster wound healing (within 8 days) which is evident through reduction in pain, swelling, discharge and granulation tissue score along with superior fly repellent activity and duration, maggot repellent property. Therefore, it appears from the present study that treatment of myiasis wound with Topicure® Plus spray along with antibiotic regime was the most effective in field conditions as it avoids the day to day manual removal of maggots and induces the faster wound healing.Asian J. Med. Biol. Res. June 2017, 3(2): 216-220

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