scholarly journals PYL8 ABA receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by ABA

2020 ◽  
Author(s):  
Irene Garcia-Maquilon ◽  
Alberto Coego ◽  
Jorge Lozano-Juste ◽  
Maxim Messerer ◽  
Carlos de Ollas ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Molecular Mechanisms ◽  
Date Palm ◽  
Biochemical Characterization ◽  
Soil Water Potential ◽  
Phoenix Dactylifera ◽  
Aba Signaling ◽  
Abiotic Stress Response ◽  
Aba Receptors

Abstract The identification of those prevalent abscisic acid (ABA) receptors and molecular mechanisms that trigger drought adaptation in crops well adapted to harsh conditions such as date palm (Phoenix dactylifera, Pd) sheds light on plant–environment interactions. We reveal that PdPYL8-like receptors are predominantly expressed under abiotic stress, with Pd27 being the most expressed receptor in date palm. Therefore, subfamily I PdPYL8-like receptors have been selected for ABA signaling during abiotic stress response in this crop. Biochemical characterization of PdPYL8-like and PdPYL1-like receptors revealed receptor- and ABA-dependent inhibition of PP2Cs, which triggers activation of the pRD29B-LUC reporter in response to ABA. PdPYLs efficiently abolish PP2C-mediated repression of ABA signaling, but loss of the Trp lock in the seed-specific AHG1-like phosphatase PdPP2C79 markedly impairs its inhibition by ABA receptors. Characterization of Arabidopsis transgenic plants that express PdPYLs shows enhanced ABA signaling in seed, root, and guard cells. Specifically, Pd27-overexpressing plants showed lower ABA content and were more efficient than the wild type in lowering transpiration at negative soil water potential, leading to enhanced drought tolerance. Finally, PdPYL8-like receptors accumulate after ABA treatment, which suggests that ABA-induced stabilization of these receptors operates in date palm for efficient boosting of ABA signaling in response to abiotic stress.

scholarly journals PYL8 ABA receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by ABA (118)

2020 ◽  
Author(s):  
Irene Garcia-Maquilon ◽  
Alberto Coego ◽  
Jorge Lozano-Juste ◽  
Maxim Messerer ◽  
Carlos de Ollas ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Molecular Mechanisms ◽  
Date Palm ◽  
Biochemical Characterization ◽  
Soil Water Potential ◽  
Phoenix Dactylifera ◽  
Aba Signaling ◽  
Abiotic Stress Response ◽  
Aba Receptors

AbstractThe identification of those prevailing ABA receptors and molecular mechanisms that trigger drought adaptation in crops well adapted to harsh conditions such as date palm (Phoenix dactylifera, Pd) sheds light on plant-environment interactions. We reveal that PdPYL8-like receptors are predominantly expressed under abiotic stress, being Pd27 the most expressed receptor in date palm. Therefore, subfamily I PdPYL8-like receptors have been selected for ABA signaling during abiotic stress response in this crop. Biochemical characterization of PdPYL8-like and PdPYL1-like receptors revealed receptor- and ABA-dependent inhibition of PP2Cs, which triggers activation of the pRD29B-LUC reporter in response to ABA. PdPYLs efficiently abolish PP2C-mediated repression of ABA signaling, but loss of the Trp lock in the seed-specific AHG1-like phosphatase PdPP2C79 markedly impairs its inhibition by ABA receptors. Characterization of Arabidopsis transgenic plants that express PdPYLs shows enhanced ABA signaling in seed, root and guard cells. Specifically, Pd27 overexpressing (OE) plants showed lower ABA content and were more efficient than wild type to lower transpiration at negative soil water potential, leading to enhanced drought tolerance. Finally, PdPYL8-like receptors accumulate after ABA treatment, which suggests that ABA-induced stabilization of these receptors operates in date palm for efficient boosting of ABA signaling in response to abiotic stress.HighlightDate palm response to abiotic stress is triggered through PYL8-like ABA receptors that are stabilized by the hormone, which boosts plant adaptation to drought mediated by ABA.

scholarly journals A Concerted Action of UBA5 C-Terminal Unstructured Regions Is Important for Transfer of Activated UFM1 to UFC1

2021 ◽  
Vol 22 (14) ◽  
pp. 7390
Author(s):  
Nicole Wesch ◽  
Frank Löhr ◽  
Natalia Rogova ◽  
Volker Dötsch ◽  
Vladimir V. Rogov
Keyword(s):  
Cellular Localization ◽  
Molecular Mechanisms ◽  
Biochemical Characterization ◽  
Effective Interaction ◽  
Regulatory Region ◽  
Titration Calorimetry ◽  
Enzymatic Reactions ◽  
Cellular Processes ◽  
Mechanism Of Interaction

Ubiquitin fold modifier 1 (UFM1) is a member of the ubiquitin-like protein family. UFM1 undergoes a cascade of enzymatic reactions including activation by UBA5 (E1), transfer to UFC1 (E2) and selective conjugation to a number of target proteins via UFL1 (E3) enzymes. Despite the importance of ufmylation in a variety of cellular processes and its role in the pathogenicity of many human diseases, the molecular mechanisms of the ufmylation cascade remains unclear. In this study we focused on the biophysical and biochemical characterization of the interaction between UBA5 and UFC1. We explored the hypothesis that the unstructured C-terminal region of UBA5 serves as a regulatory region, controlling cellular localization of the elements of the ufmylation cascade and effective interaction between them. We found that the last 20 residues in UBA5 are pivotal for binding to UFC1 and can accelerate the transfer of UFM1 to UFC1. We solved the structure of a complex of UFC1 and a peptide spanning the last 20 residues of UBA5 by NMR spectroscopy. This structure in combination with additional NMR titration and isothermal titration calorimetry experiments revealed the mechanism of interaction and confirmed the importance of the C-terminal unstructured region in UBA5 for the ufmylation cascade.

scholarly journals Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sridevi Muppala ◽  
Pavan Kumar Gudlavalleti ◽  
Kodandarami Reddy Malireddy ◽  
Sateesh Kumar Puligundla ◽  
Premalatha Dasari
Keyword(s):  
Abiotic Stress ◽  
Inbred Line ◽  
Biochemical Characterization ◽  
Abiotic Stress Tolerance ◽  
Crop Improvement ◽  
Maximum Frequency ◽  
Aba Signaling ◽  
Tolerant Plants ◽  
Maize Plants ◽  
Gateway Technology

Abstract Background In crop plants, to cope up with the demand of food for rising population, revolutionary crop improvement programmes are being implemented for higher and higher yields. Abiotic stress, especially at flowering stage, causes drastic effect on yield in plants. Deforestation and urbanization made the water table very low and changed the climate which led to untimely and unforeseen rains which affect the yield of a crop through stress, both by lack of water as well as water logging (abiotic stress). Development of tolerant plants through breeding is a time-consuming programme and does not perform well in normal conditions. Development of stress-tolerant plants through transgenic technology is the better solution. Maize is a major crop used as food and fodder and has the commercial value in ethanol production. Hence, the genes viz., nced (9-cis-epoxycarotenoid dioxygenase) and rpk (receptor-like protein kinase), which play the key roles in the abscisic acid pathway and upstream component in ABA signaling have been transferred into maize plants through Agrobacterium-mediated transformation by optimizing several parameters to obtain maximum frequency of transformation. Results Cultures raised from immature embryos of 2-mm size isolated from maize cobs, 12–15 days after pollination, were used for transformation. rpk and nced genes under the control of leaP and salT promoters respectively, cloned using gateway technology, have been introduced into elite maize inbred lines. Maximum frequency of transformation was observed with the callus infected after 20 days of inoculation by using 100 μM acetosyringone, 10 min infection time, and 2 days incubation period after co-cultivation resulted in maximum frequency of transformation (6%) in the NM5884 inbred line. Integration of the genes has been confirmed with molecular characterization by performing PCRs with marker as well as gene-specific primers and through southern hybridization. Physiological and biochemical characterization was done in vitro (artificial stress) and in vivo (pot experiments). Conclusions Changes in the parameters which affect the transformation frequency yielded maximum frequency of transformation with 20-day-old callus in the NM5884 inbred line. Introducing two or more genes using gateway technology is useful for developing stable transgenic plants with desired characters, abiotic stress tolerance in this study.

Development and characterization of polyvinyl alcohol filled with date palm midrib powder

2021 ◽  
pp. 089270572110633
Author(s):  
Yunusa Umar ◽  
SK Manirul Haque ◽  
Mamdouh Al-Harthi ◽  
Zakariya Sadique ◽  
Omar Ashwaq ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Date Palm ◽  
Tensile Modulus ◽  
Phoenix Dactylifera ◽  
Filler Loading ◽  
Phoenix Dactylifera L ◽  
Inverse Effect ◽  
Solution Casting Method ◽  
Thermal Stability Of

Date palm ( Phoenix dactylifera L) is one of the most widely cultivated crops in different parts of Saudi Arabia. The midribs rich in cellulose, hemicellulose, and lignin are often burnt in the farms, causes severe environmental problems. In the present study, date palm midrib (DPM), these waste materials were powdered and incorporated into polyvinyl alcohol (PVA). Composites were prepared by varying the DPM loading (0–10 wt%) using the solution casting method. Tensile and thermal properties were analyzed for the composites with respect to filler loading. Addition of DPM as filler enhanced the tensile modulus while an inverse effect was observed in the elongation values. Differential scanning calorimeter (DSC) analysis showed a gradual increase in melting temperature (Tm) and crystallinity values for PVA. Thermogravimetric analysis (TGA) indicated that the incorporation of DPM into PVA can increase the thermal stability of PVA. Morphology of the composites were performed using scanning electron microscopy (SEM).

Recent understanding on molecular mechanisms of plant abiotic stress response and tolerance.

2021 ◽  
pp. 1-23
Author(s):  
Geoffrey Onaga ◽  
Kerstin Wydra
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stresses ◽  
Crop Production ◽  
Molecular Mechanisms ◽  
Abiotic Stress Response ◽  
Molecular Components ◽  
Plant Abiotic Stress

Abstract This chapter provides an overview of the recent significant perspectives on molecules involved in response and tolerance to drought and salinity, the 2 major abiotic stresses affecting crop production, and highlights major molecular components identified in major cereals.

scholarly journals Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties

2014 ◽  
Vol 38 ◽  
pp. 1157-1168 ◽  
Author(s):  
Mohsen HANANA ◽  
Samia DALDOUL ◽  
Romain FOUQUET ◽  
Laurent DELUC ◽  
Céline LEON ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response ◽  
Identification And Characterization

scholarly journals Genome-Wide Identification, Genomic Organization, and Characterization of Potassium Transport-Related Genes in Cajanus cajan and Their Role in Abiotic Stress

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2238
Author(s):  
Muhammad Hussnain Siddique ◽  
Naeem Iqbal Babar ◽  
Roshan Zameer ◽  
Saima Muzammil ◽  
Nazia Nahid ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Cajanus Cajan ◽  
Dry Weight ◽  
Regulatory Elements ◽  
Chromosomal Mapping ◽  
Abiotic Stress Response ◽  
K Channels ◽  
Inorganic Cation ◽  
K Transport

Potassium is the most important and abundant inorganic cation in plants and it can comprise up to 10% of a plant’s dry weight. Plants possess complex systems of transporters and channels for the transport of K+ from soil to numerous parts of plants. Cajanus cajan is cultivated in different regions of the world as an economical source of carbohydrates, fiber, proteins, and fodder for animals. In the current study, 39 K+ transport genes were identified in C. cajan, including 25 K+ transporters (17 carrier-like K+ transporters (KUP/HAK/KTs), 2 high-affinity potassium transporters (HKTs), and 6 K+ efflux transporters (KEAs) and 14 K+ channels (9 shakers and 5 tandem-pore K+ channels (TPKs). Chromosomal mapping indicated that these genes were randomly distributed among 10 chromosomes. A comparative phylogenetic analysis including protein sequences from Glycine max, Arabidopsis thaliana, Oryza sativa, Medicago truncatula Cicer arietinum, and C. cajan suggested vital conservation of K+ transport genes. Gene structure analysis showed that the intron/exon organization of K+ transporter and channel genes is highly conserved in a family-specific manner. In the promoter region, many cis-regulatory elements were identified related to abiotic stress, suggesting their role in abiotic stress response. Abiotic stresses (salt, heat, and drought) adversely affect chlorophyll, carotenoids contents, and total soluble proteins. Furthermore, the activities of catalase, superoxide, and peroxidase were altered in C. cajan leaves under applied stresses. Expression analysis (RNA-seq data and quantitative real-time PCR) revealed that several K+ transport genes were expressed in abiotic stress-responsive manners. The present study provides an in-depth understanding of K+ transport system genes in C. cajan and serves as a basis for further characterization of these genes.

scholarly journals Molecular mechanisms in plant abiotic stress response

2011 ◽  
Vol 48 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Palmiro Poltronieri ◽  
Stefania Bonsegna ◽  
Domenico de ◽  
Angelo Santino
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Molecular Mechanisms ◽  
Abiotic Stress Response ◽  
Plant Abiotic Stress
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