scholarly journals Molecular responses of legumes to abiotic stress: protein post-translational modifications and redox signaling

2021 ◽  
Author(s):  
Manuel A Matamoros ◽  
Manuel Becana
Keyword(s):  
Abiotic Stress ◽  
Crop Production ◽  
Current Knowledge ◽  
Root Nodules ◽  
Stress Protein ◽  
Weather Conditions ◽  
Thiol Group ◽  
Nitrogen Fertilizers ◽  
Post Translational Modification ◽  
Change Models

Abstract Legumes include several major crops that are able to fix atmospheric nitrogen in symbiotic root nodules, thus reducing the demand for nitrogen fertilizers and contributing to sustainable agriculture. Global change models predict increases in temperature and more extreme weather conditions. This scenario might increase plant exposure to abiotic stresses and negatively affect crop production. Regulation of whole-plant physiology and nitrogen fixation in legumes during abiotic stress is complex and only a few mechanisms have been elucidated. Reactive oxygen (ROS), nitrogen (RNS), and sulfur (RSS) species are key players in the acclimation and stress tolerance of plants. However, the specific redox-dependent signaling pathways are far from understood. One mechanism by which ROS, RNS, and RSS fulfil their signaling role is the post-translational modification (PTM) of proteins. Redox-based PTMs mostly occur in the cysteine thiol group (oxidation, S-nitrosylation, S-glutathionylation, persulfidation), but also in methionine (oxidation), tyrosine (nitration), and lysine and arginine (carbonylation/glycation) residues. Unraveling PTM patterns under different types of stress and establishing the functional implications may reveal so far unknown underlying mechanisms of the plant and nodule responses to adverse conditions. Here we review the current knowledge on redox PTMs in legumes and their possible consequences in plant and nodule biology.

scholarly journals The power of seaweeds as plant biostimulants to boost crop production under abiotic stress

2021 ◽  
Author(s):  
Ayodeji Deolu-Ajayi ◽  
Ingrid van der Meer ◽  
Adrie Van der Werf ◽  
Rumyana Karlova
Keyword(s):  
Abiotic Stress ◽  
Protein Content ◽  
Crop Production ◽  
Current Knowledge ◽  
Crop Productivity ◽  
Seaweed Extracts ◽  
Molecular Mechanism Of Action ◽  
Major Factors ◽  
The Impact

In order to meet increasing food demands in the future, we will need to improve the current crop productivity. Abiotic stresses like drought and salinity are major factors resulting in crop yield losses and soil degradation worldwide. Recent studies suggest that seaweed-based biostimulants could be a solution for this problem. Here we summarise the current findings of using these biostimulants and highlight current knowledge gaps. Seaweed extracts were shown to enhance nutrient uptake and improve growth performance in crops under stressed and normal conditions. Seaweed extracts contain phytohormones, polysaccharides, polyphenols, lipids, amino acids and proteins. Although it has been shown that some of these compounds are active and have growth-promoting properties on plants, their underlying molecular mechanism of action and optimal applications especially in crops exposed to abiotic stress remains understudied. Seaweed extracts were shown to also improve protein content of crops and contribute to a healthy soil by facilitating water retention, soil aeration and nutrient availability, thereby promoting plant growth. In this paper we review the role of these extracts and their bioactive compounds as plant biostimulants. The targeted application to improve crop performance and the impact of seaweed extracts for enhancing the protein content of crops are discussed.

The questions of methodology of field experiments in agriculture and crop production

2018 ◽  
Vol 1 (94) ◽  
pp. 38-44
Author(s):  
А.M. Malienkо ◽  
N.E. Borуs ◽  
N.G. Buslaeva
Keyword(s):  
Labor Productivity ◽  
Crop Production ◽  
Field Experiments ◽  
Weather Conditions ◽  
High Accuracy ◽  
Experimental Plot ◽  
Increase Labor Productivity ◽  
Biometric Parameters ◽  
Field Crops ◽  
High Condition

In the article, the results of research on the methodology for conducting studies with corn culture under various methods of sowing and weather conditions. The aim of the research was to establish and evaluate the reliability and high accuracy of the experiment, with a decrease in the area's acreage and taking one plant per repetition. Based on the results of the analysis of biometric parameters and yields, the possibility of sampling from 5 to 108 plants was established statistically and mathematically to establish the accuracy of the experiment. The established parameters of sites in experiments with maize indicate the possibility of obtaining much more information from a smaller unit of area, that is, to increase labor productivity not only with tilled crops. This is the goal of further scientific research with other field crops taking 1 plant of repetitions, observing the conditions of leveling the experimental plot according to the fertility of the soil and sowing seeds with high condition. The data obtained give grounds for continuing research on the minimum space required and the sample in the experiments.

scholarly journals Malonyl-proteome profiles of Staphylococcus aureus reveal lysine malonylation modification in enzymes involved in energy metabolism

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yanan Shi ◽  
Jingjing Zhu ◽  
Yan Xu ◽  
Xiaozhao Tang ◽  
Zushun Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Energy Metabolism ◽  
Active Sites ◽  
Current Knowledge ◽  
Tricarboxylic Acid Cycle ◽  
Protein A ◽  
Pentose Phosphate ◽  
Post Translational Modification ◽  
Bacterial Physiology ◽  
Dihydrolipoyl Dehydrogenase

Abstract Background Protein lysine malonylation, a novel post-translational modification (PTM), has been recently linked with energy metabolism in bacteria. Staphylococcus aureus is the third most important foodborne pathogen worldwide. Nonetheless, substrates and biological roles of malonylation are still poorly understood in this pathogen. Results Using anti-malonyl-lysine antibody enrichment and high-resolution LC-MS/MS analysis, 440 lysine-malonylated sites were identified in 281 proteins of S. aureus strain. The frequency of valine in position − 1 and alanine at + 2 and + 4 positions was high. KEGG pathway analysis showed that six categories were highly enriched, including ribosome, glycolysis/gluconeogenesis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA), valine, leucine, isoleucine degradation, and aminoacyl-tRNA biosynthesis. In total, 31 malonylated sites in S. aureus shared homology with lysine-malonylated sites previously identified in E. coli, indicating malonylated proteins are highly conserved among bacteria. Key rate-limiting enzymes in central carbon metabolic pathways were also found to be malonylated in S. aureus, namely pyruvate kinase (PYK), 6-phosphofructokinase, phosphoglycerate kinase, dihydrolipoyl dehydrogenase, and F1F0-ATP synthase. Notably, malonylation sites were found at or near protein active sites, including KH domain protein, thioredoxin, alanine dehydrogenase (ALD), dihydrolipoyl dehydrogenase (LpdA), pyruvate oxidase CidC, and catabolite control protein A (CcpA), thus suggesting that lysine malonylation may affect the activity of such enzymes. Conclusions Data presented herein expand the current knowledge on lysine malonylation in prokaryotes and indicate the potential roles of protein malonylation in bacterial physiology and metabolism.

scholarly journals Winter Wheat Straw Decomposition under Different Nitrogen Fertilizers

Agriculture ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 83
Author(s):  
Gabriela Mühlbachová ◽  
Pavel Růžek ◽  
Helena Kusá ◽  
Radek Vavera ◽  
Martin Káš
Keyword(s):  
Winter Wheat ◽  
Wheat Straw ◽  
Soil Surface ◽  
Weather Conditions ◽  
Mineral Nitrogen ◽  
Nitrogen Fertilizers ◽  
Pig Slurry ◽  
Straw Decomposition ◽  
Rainy Weather ◽  
N Fertilisers

The climate changes and increased drought frequency still more frequent in recent periods bring challenges to management with wheat straw remaining in the field after harvest and to its decomposition. The field experiment carried out in 2017–2019 in the Czech Republic aimed to evaluate winter wheat straw decomposition under different organic and mineral nitrogen fertilizing (urea, pig slurry and digestate with and without inhibitors of nitrification (IN)). Treatment Straw 1 with fertilizers was incorporated in soil each year the first day of experiment. The Straw 2 was placed on soil surface at the same day as Straw 1 and incorporated together with fertilizers after 3 weeks. The Straw 1 decomposition in N treatments varied between 25.8–40.1% and in controls between 21.5–33.1% in 2017–2019. The Straw 2 decomposition varied between 26.3–51.3% in N treatments and in controls between 22.4–40.6%. Higher straw decomposition in 2019 was related to more rainy weather. The drought observed mainly in 2018 led to the decrease of straw decomposition and to the highest contents of residual mineral nitrogen in soils. The limited efficiency of N fertilisers on straw decomposition under drought showed a necessity of revision of current strategy of N treatments and reduction of N doses adequately according the actual weather conditions.

COMPARISON OF THREE CONTROLLED-RELEASE NITROGEN FERTILIZERS IN GREENHOUSE CROP PRODUCTION

2002 ◽  
Vol 25 (5) ◽  
pp. 1049-1061 ◽  
Author(s):  
Eugene K. Blythe ◽  
Joshua L. Mayfield ◽  
Barrett C. Wilson ◽  
Edgar L. Vinson III ◽  
Jeff L. Sibley
Keyword(s):  
Controlled Release ◽  
Crop Production ◽  
Nitrogen Fertilizers

Selection for Some Functional Markers for Adaptability of Helianthus argophyllus × Helianthus annuus Derived Population under Abiotic Stress Conditions

Helia ◽  
2018 ◽  
Vol 41 (68) ◽  
pp. 83-108 ◽  
Author(s):  
Muhammad Mubashar Hussain ◽  
Maria Kausar ◽  
Saeed Rauf ◽  
M. Farukh Zafar Khan ◽  
Jakub Paderweski ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Cell Membrane ◽  
Crop Production ◽  
Functional Markers ◽  
High Fertility ◽  
Epicuticular Waxes ◽  
Membrane Injury ◽  
Leaf Hairiness ◽  
Selection Of

AbstractAbiotic stresses including drought are major crop production constraints. However, specific functional phenotypic markers induce resistance against these stresses. Therefore, a study was initiated to study the variability, inheritance and selection of epicuticular waxes (EW) and leaf hairiness (LH) along with low cell membrane injuries (CMI) within F2 populations derived by crossing H. annuus×H. argophyllus lines. These traits have been shown to be associated with drought tolerance of Helianthus argophyllus and thus study aims to introgress these traits in Helinathus annuus. The studied parent populations showed contrasting values of the traits. The drought susceptible line CMS-14 and CMS-20 showed lower epicuticular waxes (0.79, 0.69 mg g−1), leaf hairiness (0.75, 1.53) and higher cell membrane injury (40.90, 55.76 %) respectively while drought resistant line Argo 1802 and 1806 showed higher epicuticular waxes (2.28, 3.18), leaf hairiness (3.71, 3.80) and lower cell membrane injury (14.22, 21.54 %) respectively. The F1 hybrids had mean values of the three studied parameters i. e. epicuticular waxes (1.50 mg g−1), cell membrance injury (32.54 %) and leaf hairiness (2.74) in the range of parent lines, but some of F2 individuals extend beyond this range (Parents and F1s). The two-step selections maintained high variability especially of LH for set of F2 individuals (H. annuus CMS-20×H. argophyllus 1806). Simultaneous selection of F2 individuals with high values of LH or EW with low CMI was possible. The selected plants were further studied for narrow leaf, high fertility and silver canopy color. Selected material was promoted as the candidate of inbred line. Plant (F4) having introgressed traits (silver canopy) showed lower yield (19 %) than green leafed plants (53 %) and commercial hybrids under drought stress (63 % and 53 %). The study could help to increase the abiotic stress tolerance, minimize the yield losses under drought stress and increase functional diversity within sunflower.

scholarly journals Contribution of Arbuscular Mycorrhizal Fungi, Phosphate–Solubilizing Bacteria, and Silicon to P Uptake by Plant

2021 ◽  
Vol 12 ◽  
Author(s):  
Hassan Etesami ◽  
Byoung Ryong Jeong ◽  
Bernard R. Glick
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Crop Production ◽  
Current Knowledge ◽  
Arbuscular Mycorrhizal ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Phosphate Solubilizing ◽  
P Fertilizers

Phosphorus (P) availability is usually low in soils around the globe. Most soils have a deficiency of available P; if they are not fertilized, they will not be able to satisfy the P requirement of plants. P fertilization is generally recommended to manage soil P deficiency; however, the low efficacy of P fertilizers in acidic and in calcareous soils restricts P availability. Moreover, the overuse of P fertilizers is a cause of significant environmental concerns. However, the use of arbuscular mycorrhizal fungi (AMF), phosphate–solubilizing bacteria (PSB), and the addition of silicon (Si) are effective and economical ways to improve the availability and efficacy of P. In this review the contributions of Si, PSB, and AMF in improving the P availability is discussed. Based on what is known about them, the combined strategy of using Si along with AMF and PSB may be highly useful in improving the P availability and as a result, its uptake by plants compared to using either of them alone. A better understanding how the two microorganism groups and Si interact is crucial to preserving soil fertility and improving the economic and environmental sustainability of crop production in P deficient soils. This review summarizes and discusses the current knowledge concerning the interactions among AMF, PSB, and Si in enhancing P availability and its uptake by plants in sustainable agriculture.

scholarly journals CLIMATE CHANGE IMPACT ON MOUNTAIN BIODIVERSITY: A SPECIAL REFERENCE TO GILGIT-BALTISTAN OF PAKISTAN

2016 ◽  
Vol 1 ◽  
pp. 53
Author(s):  
S. Ishaq ◽  
M. Z. Khan ◽  
F. Begum ◽  
K. Hussain ◽  
R. Amir ◽  
...  
Keyword(s):  
Climate Change ◽  
Natural Resources ◽  
Special Reference ◽  
Current Knowledge ◽  
Knowledge Gap ◽  
Change Models ◽  
Impact Of Climate Change ◽  
The Government ◽  
Gilgit Baltistan ◽  
The Impact

Climate Change is not a stationary phenomenon; it moves from time to time, it represents a major threat to mountainous biodiversity and to ecosystem integrity. The present study is an attempt to identify the current knowledge gap and the effects of climate change on mountainous biodiversity, a special reference to the Gilgit-Baltistan is briefly reviewed. Measuring the impact of climate change on mountain biodiversity is quite challenging, because climate change interacts with every phenomenon of ecosystem. The scale of this change is so large and very adverse so strongly connected to ecosystem services, and all communities who use natural resources. This study aims to provide the evidences on the basis of previous literature, in particular context to mountain biodiversity of Gilgit-Baltistan (GB). Mountains of Gilgit-Baltistan have most fragile ecosystem and are more vulnerable to climate change. These mountains host variety of wild fauna and flora, with many endangered species of the world. There are still many gaps in our knowledge of literature we studied because very little research has been conducted in Gilgit-Baltistan about climate change particular to biodiversity. Recommendations are made for increased research efforts in future this including jointly monitoring programs, climate change models and ecological research. Understanding the impact of climate change particular to biodiversity of GB is very important for sustainable management of these natural resources. The Government organizations, NGOs and the research agencies must fill the knowledge gap, so that it will help them for policy making, which will be based on scientific findings and research based.

scholarly journals The Effect of Abiotic Stresses on the Protein Composition of Four Hungarian Wheat Varieties

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
Dalma Nagy-Réder ◽  
Zsófia Birinyi ◽  
Marianna Rakszegi ◽  
Ferenc Békés ◽  
Gyöngyvér Gell
Keyword(s):  
Abiotic Stress ◽  
Protein Content ◽  
Functional Properties ◽  
Crop Production ◽  
Global Climate ◽  
Protein Composition ◽  
Grain Protein Content ◽  
Wheat Varieties ◽  
Significant Difference ◽  
Negative Effect

Global climate change in recent years has resulted in extreme heat and drought events that significantly influence crop production and endanger food security. Such abiotic stress during the growing season has a negative effect on yield as well as on the functional properties of wheat grain protein content and composition. This reduces the value of grain, as these factors significantly reduce end-use quality. In this study, four Hungarian bread wheat cultivars (Triticum aestivum ssp. aestivum) with different drought and heat tolerance were examined. Changes in the size- and hydrophobicity-based distribution of the total proteins of the samples have been monitored by SE- and RP-HPLC, respectively, together with parallel investigations of changes in the amounts of the R5 and G12 antibodies related to celiac disease immunoreactive peptides. Significant difference in yield, protein content and composition have been observed in each cultivar, altering the amounts of CD-related gliadin, as well as the protein parameters directly related to techno-functional properties (Glu/Gli ratio, UPP%). The extent of changes largely depended on the timing of the abiotic stress. The severity of the negative effect depended on the growth stage in which abiotic stress occurred.

Sign in / Sign up

Export Citation Format

Share Document