Diverse families of transposable elements affect the transcriptional regulation of stress-response genes in Drosophila melanogaster

It was investigated the role of stress-response genes (dFOXO, dSir2, Hsp70) in regulation of life span of Drosophila in response to light regime alteration. It was revealed the FOXO-dependant mechanism of lifespan increasing at darkness conditions. The distance of lifespan of FOXO homozygous mutants at different light conditions were absent 3 times from 4 times. It was shown, that homozygotes with deletion of dSir2 have more significant difference between lifespan at standard light and darkness conditions with comparing to wild type and heterozygous strain. The same tendency was also detected the in the strains with Hsp70 deletions. It was produced the evidences of two mechanisms of light regime influence on lifespan: metabolism intensification at light conditions and neuroendocrine-determinated lifespan increasing at darkness conditions.

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AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis

Scientific Reports ◽

10.1038/s41598-020-80695-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yanxing Ma ◽

Hainan Tian ◽

Rao Lin ◽

Wei Wang ◽

Na Zhang ◽

...

Keyword(s):

Stress Response ◽

Protein Phosphatase ◽

Aba Signaling ◽

Response Gene ◽

Stress Response Genes ◽

Arabidopsis Protein ◽

Evolutionarily Conserved ◽

Increased Sensitivity ◽

Aba Response ◽

Transcription Repressors

AbstractExpression of stress response genes can be regulated by abscisic acid (ABA) dependent and ABA independent pathways. Osmotic stresses promote ABA accumulation, therefore inducing the expression of stress response genes via ABA signaling. Whereas cold and heat stresses induce the expression of stress response genes via ABA independent pathway. ABA induced transcription repressors (AITRs) are a family of novel transcription factors that play a role in ABA signaling, and Drought response gene (DRG) has previously been shown to play a role in regulating plant response to drought and freezing stresses. We report here the identification of DRG as a novel transcription factor and a regulator of ABA response in Arabidopsis. We found that the expression of DRG was induced by ABA treatment. Homologs searching identified AITR5 as the most closely related Arabidopsis protein to DRG, and homologs of DRG, including the AITR-like (AITRL) proteins in bryophytes and gymnosperms, are specifically presented in embryophytes. Therefore we renamed DRG as AITRL. Protoplast transfection assays show that AITRL functioned as a transcription repressor. In seed germination and seedling greening assays, the aitrl mutants showed an increased sensitivity to ABA. By using qRT-PCR, we show that ABA responses of some ABA signaling component genes including some PYR1-likes (PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs) and SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASES 2s (SnRK2s) were reduced in the aitrl mutants. Taken together, our results suggest that AITRLs are a family of novel transcription repressors evolutionally conserved in embryophytes, and AITRL regulates ABA response in Arabidopsis by affecting ABA response of some ABA signaling component genes.

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Transcriptional response of key metabolic and stress response genes of a nuculanid bivalve, Lembulus bicuspidatus from an oxygen minimum zone exposed to hypoxia-reoxygenation

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2021.110617 ◽

2021 ◽

pp. 110617

Author(s):

Katherine Amorim ◽

Helen Piontkivska ◽

Michael L. Zettler ◽

Eugene Sokolov ◽

Tjorven Hinzke ◽

...

Keyword(s):

Stress Response ◽

Transcriptional Response ◽

Oxygen Minimum Zone ◽

Stress Response Genes ◽

Minimum Zone ◽

Oxygen Minimum ◽

Hypoxia Reoxygenation

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Chromosomal Distribution of Transposable Elements in Drosophila melanogaster Test of the Ectopic Recombination Model for Maintenance of Insertion Site Number

Genetics ◽

10.1093/genetics/144.1.197 ◽

1996 ◽

Vol 144 (1) ◽

pp. 197-204

Author(s):

Christine Hoogland ◽

Christian Biémont

Keyword(s):

Drosophila Melanogaster ◽

Transposable Elements ◽

Dna Content ◽

Recombination Rate ◽

Alternative Hypothesis ◽

Insertion Site ◽

Polytene Chromosomes ◽

Negative Relationship ◽

Site Occupancy ◽

Site Number

Abstract Data of insertion site localization and site occupancy frequency of P, hobo, I, copia, mdg1, mdg3, 412, 297, and roo transposable elements (TEs) on the polytene chromosomes of Drosophila melanogaster were extracted from the literature. We show that TE insertion site number per chromosomal division was significantly correlated with the amount of DNA. The insertion site number weighted by DNA content was not correlated with recombination rate for all TEs except hobo, for which a positive correlation was detected. No global tendency emerged in the relationship between TE site occupancy frequency, weighted by DNA content, and recombination rate; a strong negative correlation was, however, found for the 3L arm. A possible dominant deleterious effect of chromosomal rearrangements due to recombination between TE insertions is thus not the main factor explaining the dynamics of TEs, since this hypothesis implies a negative relationship between recombination rate and both TE insertion site number and site occupancy frequency. The alternative hypothesis of selection against deleterious effects of insertional mutations is discussed.

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Cellular internalization and stress response of ingested amorphous silica nanoparticles in the midgut of Drosophila melanogaster

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2012.10.001 ◽

2013 ◽

Vol 1830 (1) ◽

pp. 2256-2266 ◽

Cited By ~ 32

Author(s):

Ashutosh Pandey ◽

Swati Chandra ◽

Lalit Kumar Singh Chauhan ◽

Gopeshwar Narayan ◽

Debapratim Kar Chowdhuri

Keyword(s):

Drosophila Melanogaster ◽

Stress Response ◽

Silica Nanoparticles ◽

Amorphous Silica ◽

Cellular Internalization

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Polymorphisms in stress response genes in Lactobacillus plantarum: implications for classification and heat stress response

Annals of Microbiology ◽

10.1007/s13213-014-0862-7 ◽

2014 ◽

Vol 65 (1) ◽

pp. 297-305

Author(s):

Angela Guidone ◽

Eugenio Parente ◽

Teresa Zotta ◽

Caitriona M. Guinane ◽

Mary C. Rea ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Lactobacillus Plantarum ◽

Heat Stress Response ◽

Stress Response Genes

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Vitamin D regulates the expression of immune and stress response genes in dengue virus-infected macrophages by inducing specific microRNAs

MicroRNA ◽

10.2174/2211536610666211221151949 ◽

2021 ◽

Vol 11 ◽

Author(s):

Geysson Javier Fernandez ◽

Jorge Andrés Castillo ◽

Diana Marcela Giraldo ◽

Silvio Urcuqui-Inchima

Keyword(s):

Vitamin D ◽

Immune Response ◽

Stress Response ◽

Dengue Virus ◽

Mirna Expression ◽

Expression Profiles ◽

Denv Infection ◽

High Dose ◽

Regulatory Pathways ◽

Stress Response Genes

Background: The pathogenesis associated with Dengue virus (DENV) infection is marked by the impairment of host immune response. Consequently, the modulation of immune response has emerged as an important therapeutic target for the control of DENV infection. Vitamin D has been shown to regulate the immune response in DENV infection, although the molecular mechanism remains poorly understood. Post-transcriptional regulation of mRNA by miRNAs offers an opportunity to gain insight into the immunomodulation mediated by vitamin D Objective: Previously, it has been observed that a high dose of vitamin D (4000 IU) decreased DENV-2 infection and inflammatory response in monocyte-derived macrophages (MDMs). Here, we examine whether high or low doses of vitamin D supplements exert differential effect on miRNA expression in DENV-infected macrophages Methods: We analyzed miRNA expression profiles in MDMs isolated from healthy individuals who were given either 1000 or 4000 IU/day of vitamin D for 10 days. MDMs before or after vitamin D supplementation were challenged with DENV-2, and miRNAs profiles were analyzed by qPCR arrays. Results: DENV-2 infected MDMs supplemented with 4000 IU, showed up-regulation of miR-374a-5p, miR-363-3p, miR-101-3p, miR-9-5p, miR-34a-5p, miR-200a-3p, and the family of miRNAs miR-21-5p, and miR-590-p. The miRNA profile and predicted target mRNAs suggested regulatory pathways in MDMs obtained from healthy donors who received higher doses of vitamin D. These DENV-2 infected MDMs expressed a unique set of miRNAs that target immune and cellular stress response genes. Conclusion: The results suggest vitamin D dose-dependent differential expression of miRNAs target key signaling pathways of the pathogenesis of dengue disease.

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Anti-oxidative stress response genes: bioinformatic analysis of their expression and relevance in multiple cancers

Oncotarget ◽

10.18632/oncotarget.1658 ◽

2013 ◽

Vol 4 (12) ◽

pp. 2577-2590 ◽

Cited By ~ 27

Author(s):

Barak Rotblat ◽

Thomas G. P. Grunewald ◽

Gabriel Leprivier ◽

Gerry Melino ◽

Richard A. Knight

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Bioinformatic Analysis ◽

Oxidative Stress Response ◽

Stress Response Genes ◽

Multiple Cancers

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PICH, an ATP-dependent chromatin remodeling protein, transcriptionally co-regulates oxidative stress response.

10.1101/2021.07.21.453306 ◽

2021 ◽

Author(s):

Anindita Dutta ◽

Apurba Das ◽

Deep Bisht ◽

Vijendra Arya ◽

Rohini Muthuswami

Keyword(s):

Oxidative Stress ◽

Transcription Factor ◽

Transcriptional Regulation ◽

Stress Response ◽

Chromatin Remodeling ◽

Dna Sequences ◽

Target Genes ◽

Antioxidant Response ◽

Oxidative Stress Response ◽

Antioxidant Genes

Cells respond to oxidative stress by elevating the levels of antioxidants, signaling, and transcriptional regulation often implemented by chromatin remodeling proteins. The study presented in this paper shows that the expression of PICH, an ATP-dependent chromatin remodeler, is upregulated during oxidative stress in HeLa cells. We also show that PICH regulates the expression of Nrf2, a transcription factor regulating antioxidant response, both in the absence and presence of oxidative stress. In turn, Nrf2 regulates the expression of PICH in the presence of oxidative stress. Both PICH and Nrf2 together regulate the expression of antioxidant genes and this transcriptional regulation is dependent on the ATPase activity of PICH. In addition, H3K27ac modification also plays a role in activating transcription in the presence of oxidative stress. Co-immunoprecipitation experiments show that PICH and Nrf2 interact with H3K27ac in the presence of oxidative stress. Mechanistically, PICH recognizes ARE sequences present on its target genes and introduces a conformational change to the DNA sequences leading us to hypothesize that PICH regulates transcription by remodeling DNA. PICH ablation leads to reduced expression of Nrf2 and impaired antioxidant response leading to increased ROS content, thus, showing PICH is essential for the cell to respond to oxidative stress.

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