scholarly journals CSIG-16. SEXUAL DIMPORHISM IN IRON ACQUISITION IN GLIOBLASTOMA

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii31-ii31
Author(s):  
Bhavyata Pandya ◽  
Becky Slagle-Webb ◽  
Darya Nesterova ◽  
Brad Zacharia ◽  
Justin Lathia ◽  
...  
Keyword(s):  
Iron Transport ◽  
Iron Acquisition ◽  
Poor Survival ◽  
Orthotopic Mouse Model ◽  
Tissue Samples ◽  
Male And Female ◽  
Putative Receptor ◽  
Human Male ◽  
Significant Difference ◽  
Males And Females

Abstract Glioblastoma (GBM) is the most aggressive brain cancer. Sex differences in incidence and clinical outcomes have been reported, however, our knowledge of contributing mechanisms is limited. Iron acquisition is key to robust tumor growth. Upregulation of Transferrin (Tf, iron transport protein)/Transferrin receptor (TfR) is found in multiple different cancers. We have identified H-ferritin (FTH1) as involved in iron transport and explore its uptake in GBM in this study. We interrogated iron uptake in a syngeneic orthotopic mouse model (GL261 cells) using male and female mice. After the tumors were established, radioactive 125I labeled Tf and FTH1 proteins were injected retro-orbitally in the mice. After 24 hours, tumors were removed, homogenized and analyzed for Tf and FTH1 uptake. There was a significant difference in Tf uptake into the tumor versus matched non-tumor tissue in both males and females and the uptake in the tumors was 1.5-fold higher in males than females. There was no significant difference in FTH1 uptake between male and female tumors nor between tumor and matched non-tumor brain tissue. Binding analyses were performed on homogenized samples of human male and female GBM tissue samples using 125I labeled Tf and FTH1. Tumors from males had increased binding of both proteins compared to tumors from females. We next queried the TCGA database and found in females, high TfR expression was associated with poor survival but not in males. TCGA database revealed a robust expression of Tim1, a putative receptor for FTH1, but its expression did not relate to survival. In summary, this study demonstrates FTH1 binding to GBMs and sexual dimorphism in iron acquisition via Tf and survival.

TAMI-54. SEXUAL DIMORPHISM IN IRON ACQUISITION IN GLIOBLASTOMA

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi209-vi209
Author(s):  
Bhavyata Pandya ◽  
Becky Slagle Webb ◽  
Brad Zacharia ◽  
Justin Lathia ◽  
Joshua Rubin ◽  
...  
Keyword(s):  
Sexual Dimorphism ◽  
Iron Uptake ◽  
Iron Transport ◽  
Iron Acquisition ◽  
Wild Type ◽  
Binding Studies ◽  
Orthotopic Mouse Model ◽  
Male And Female ◽  
Significant Difference

Abstract Sexual dimorphism in incidence and the clinical outcomes of Glioblastoma (GBM) has been reported, however, our knowledge of contributing biological mechanisms is limited. Iron acquisition is key to robust tumor growth. Upregulation of Transferrin (TF, iron transport protein)/Transferrin receptor (TFR) is critical for found in multiple different cancers, specifically, we have identified H-ferritin (FTH1) as a contributor to iron transport and protection in cancer stem cells. To interrogate brain tumor iron uptake mechanisms,we performed binding studies on homogenized samples of human male and female GBM tissue samples using 125I labeled TF and FTH1. Tumors from males had a ̴ 3.8-fold increased binding of both proteins compared to tumors from females. We interrogated iron uptake in a syngeneic orthotopic mouse model (GL261 cells) using male and female mice. After the tumors were established, radioactive 125I labeled TF and FTH1 proteins were injected retro-orbitally in the mice. After 24 hours, tumors wereremoved, and analyzed for TF and FTH1 uptake. Male tumors showed an increased uptake, of ̴ 3.2-fold, as compared to female tumors. There was no significant difference in TF uptake between male and female tumors nor between tumor and matched non-tumor brain tissue. We next queried role of FTH1 in the context of sexual dimorphism in GBM in a FTH1+/- mouse strain developed in our laboratory. Survival was monitored in the mice which were injected with GL261 cells at 3 months. Male mice that had reduced expression of FTH1 had poorer survival as compared to the male wild type controls whereas wild type and FTH+/- females had no major differences in survival outcomes. In summary, this study demonstrates sexual dimorphism in iron acquisition in GBM and animal models further suggesting a pathophysiological role of iron metabolism in GBM development and its possible role in prognosis.

scholarly journals Inter-Limb Jump Asymmetries and Their Association with Sport-Specific Performance in Young Male and Female Swimmers

2021 ◽  
Vol 18 (14) ◽  
pp. 7324
Author(s):  
Max I. Phukan ◽  
Rohit K. Thapa ◽  
Gopal Kumar ◽  
Chris Bishop ◽  
Helmi Chaabene ◽  
...  
Keyword(s):  
National Level ◽  
Young Male ◽  
Front Crawl ◽  
Male And Female ◽  
Standing Long Jump ◽  
Significant Difference ◽  
Specific Performance ◽  
Long Jump ◽  
Males And Females ◽  
Level Of Agreement

This study aimed to examine inter-limb jump asymmetries and their association with sport-specific performance in young swimmers. Thirty-eight (male, n = 19; female, n = 19) regional/national level young swimmers (age: 12.3 ± 1.2 years; height: 159.6 ± 8.2 cm; body mass: 52.5 ± 9.2 kg) participated in this study. Inter-limb asymmetries were assessed for single-leg countermovement jump (SLCMJ) and single-leg standing long jump (SLSLJ). Sport-specific performance was evaluated using front crawl (i.e., 50 m and 25 m) and front crawl kick (i.e., 50 m and 25 m). The kappa coefficient revealed a “slight” level of agreement (Κ = 0.156, 0.184, and 0.197 for female, male, and all, respectively) between the direction of asymmetry for SLCMJ and SLSLJ, indicating that asymmetries rarely favored the same limb during both tests. A paired sample t-test showed a significant difference (p = 0.025) between asymmetry scores obtained in SLCMJ and SLSLJ. No significant difference was found in asymmetry scores between males and females (p = 0.099 to 0.977). Additionally, no association between asymmetry scores and sport-specific performance was observed (p > 0.05). Our findings highlight the independent nature of inter-limb asymmetries derived from SLCMJ and SLSLJ among young male and female swimmers. Further, our results suggest no association between jumping asymmetries and sport-specific performance.

Distribution and abundance of Hemolivia mauritanica (Apicomplexa: Haemogregarinidae) and its vector Hyalomma aegyptium in tortoises of Iran

Biologia ◽  
2015 ◽  
Vol 70 (2) ◽  
Author(s):  
Hossein Javanbakht ◽  
Pavel Široký ◽  
Peter Mikulíček ◽  
Mozafar Sharifi
Keyword(s):  
Distribution Pattern ◽  
Age Groups ◽  
Climatic Conditions ◽  
Blood Parasite ◽  
Iranian Plateau ◽  
Male And Female ◽  
Testudo Graeca ◽  
Vector Tick ◽  
Significant Difference ◽  
Males And Females

AbstractDistribution pattern, prevalence and intensity of parasitaemia of heteroxenous apicomplexan blood parasite Hemolivia mauritanica and its vector tick Hyalomma aegyptium have been studied in 264 tortoises (212 Testudo graeca and 52 T. horsfieldii) throughout the Iranian territory. In T. graeca the highest prevalence and intensity of parasitaemia for H. mauritanica were recorded in the temperate mid and northern parts of the Iranian plateau, while the lowest values were found in the arid central part of Iran. No Hemolivia-positive samples were found in T. horsfieldii. Hyalomma aegyptium ticks were diagnosed in both tortoise species. The highest prevalence and intensity of infestation with H. aegyptium were recorded in western part of Iran, where climatic conditions are not so arid compared to central and eastern parts. No significant differences were found in intensity of parasitaemia of H. mauritanica between males and females. Similarly, no differences in intensity of parasitaemia were detected between different age groups (adults; more than 10 years, young; 5-10 years and juveniles; less that 5 years). Prevalence of H. mauritanica and H. aegyptium in adults was higher than in young and in juveniles. While intensity of infestation with H. aegyptium was significantly higher in adult tortoises compare to juveniles, no significant difference were found between intensity of H. aegyptium infestation in male and female tortoises.

scholarly journals Asphyxiated Female and Male Newborn Piglets Have Similar Outcomes With Different Cardiopulmonary Resuscitation Interventions

2020 ◽  
Vol 8 ◽  
Author(s):  
Seung Yeon Kim ◽  
Gyu-Hong Shim ◽  
Megan O'Reilly ◽  
Po-Yin Cheung ◽  
Tze-Fun Lee ◽  
...  
Keyword(s):  
Cardiopulmonary Resuscitation ◽  
Cerebral Oxygenation ◽  
Secondary Analysis ◽  
Spontaneous Circulation ◽  
Male And Female ◽  
Newborn Piglets ◽  
Sustained Inflation ◽  
Significant Difference ◽  
Tissue Homogenates ◽  
Males And Females

Background: Male newborns have a greater risk of poor cardiovascular and respiratory outcomes compared to females. The mechanisms associated with the “male disadvantage” remains unclear. We have previously shown no difference between male and female newborn piglets during hypoxia, asphyxia, resuscitation, and post-resuscitation recovery. However, it is unknown if there are differences in resuscitation outcomes between males and females during different cardiopulmonary resuscitation techniques.Intervention and Measurements: Secondary analysis of 184 term newborn mixed breed duroc piglets (1–3 days of age, weighing 2.0 (0.2) kg) from seven different studies, which were exposed to 30–50 min of normocapnic hypoxia followed by asphyxia until asystole. This was followed by cardiopulmonary resuscitation. For the analysis, piglets were divided into male and female groups, as well as resuscitation technique groups (sustained inflation, 3:1 compression-to-ventilation ratio, or asynchronous ventilations during chest compressions). Cardiac function, carotid blood flow, and cerebral oxygenation were continuously recorded throughout the experiment.Main results: Regardless of resuscitation technique, there was no significant difference between males and females in the number achieving return of spontaneous circulation (ROSC) [95/123 (77%) vs. 48/61 (79%)], the time to achieve ROSC [112 (80–185) s vs. 110 (77–186) s], and the 4-h survival rate [81/95 (85%) vs. 40/48 (83%)]. Levels of the injury markers interleukin (IL)−1ß, IL-6, IL-8, and tumor necrosis factor-α in frontoparietal cortex tissue homogenates were similar between males and females.Conclusions: Regardless of resuscitation technique, there was no significant effect of sex on resuscitation outcome, survival, and hemodynamic recovery in asphyxiated newborn piglets.

Risk-Taking Patterns of Male and Female Entrepreneurs in Roodepoort

2014 ◽  
Vol 13 (4) ◽  
pp. 452-478 ◽  
Author(s):  
Ajay K. Garg ◽  
A. Duvenhage
Keyword(s):  
Risk Taking ◽  
Business Performance ◽  
Health And Safety ◽  
Primary Data ◽  
Female Entrepreneurs ◽  
Male And Female ◽  
Significant Difference ◽  
Males And Females ◽  
The Mean ◽  
Performance Perceptions

This study analyses the risk-taking propensity of male and female entrepreneurs. Primary data were collected from a sample of 400 randomly selected from among the small and medium scale entrepreneurs in Roodepoort and two structured questionnaires were used. The results showed the existence of differences among male and female entrepreneurs’ risk-taking perceptions. However, no significant differences in the mean scores between males and females in the financial and social domains of risk taking perception were found. The results further indicate that there is a significant difference in the means of the entrepreneurs with regard to their overall risk-taking perception in the ethical domain of risk-taking perception, health and safety risk-taking perception, recreational risk-taking perception as well as their business performance perceptions. The study also suggests that there is no correlation between business performance and risk-taking in entrepreneurs. The study highlights that there is no evidence to suggest that risk-taking in different genders plays a role as an indicator in business performance.

scholarly journals a comparative analysis of canthal index of ekowe indigenes, bayelsa state

2021 ◽  
pp. 157-159
Author(s):  
Timipa Richard Ogoun ◽  
Tobia P.S S ◽  
Aye T T
Keyword(s):  
Health Care ◽  
Mean Value ◽  
Normative Values ◽  
Body Parts ◽  
Male And Female ◽  
Care Givers ◽  
Significant Difference ◽  
Males And Females ◽  
The Mean ◽  
Predictive Study

Human body parts are useful in the predictive study of the unknown. The aim of this study is to know the normative values of the canthi of the Ekowe people. Measurements such as inner and outer canthal distances were carried out. The mean value of the innercanthal distance for males and females are 3.55±0.58 and 3.43±0.52. The mean outer canthal distance for male and female is 13.91±0.84 and 13.62±0.76. The canthal index for male and female is 25.59±4.54 and 25.30±4.03. Sexual dimorphism exists and statistical significant difference was noticed in the outer canthal distance between the males and females (P˂0.05). This study has provided us with normative reference values of inner and outer canthal distances and canthal index for the Ekowe Population which is import to the health care givers, Anatomist, ophthalmic industry and anthropologist

scholarly journals An Analysis on Compliment Responses in English among Javanese People

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Arum Sekar Nurhijannah
Keyword(s):  
English Learners ◽  
T Test ◽  
Male And Female ◽  
Paired Samples ◽  
Significant Difference ◽  
Compliment Responses ◽  
Completion Test ◽  
Males And Females

The study aims at exploring the differences of compliment responses in English among Indonesian males and females. The study consists of 20 English learners in University State of Yogyakarta. The participants are expected to respond five scenarios in a modified Discourse Completion Test (DCT). The participants’ responses are submitted to SPSS and Paired Samples T-Test is applied. It reveals that each scenario has different responses but it doesn’t have significant difference among male and female participants in responding the compliments. Both male and female participants tend to agree the compliment using appreciation token.

Home-range and den use of the mahogany glider, Petaurus gracilis

2000 ◽  
Vol 27 (1) ◽  
pp. 49 ◽  
Author(s):  
Stephen M. Jackson
Keyword(s):  
Home Range ◽  
Dry Season ◽  
Home Ranges ◽  
Adjacent Area ◽  
Wet Season ◽  
Male And Female ◽  
Fragmented Habitat ◽  
Significant Difference ◽  
Socially Monogamous ◽  
Males And Females

The home-range of the mahogany glider was estimated, and its social behaviour examined, by following radio-collared animals over a two-year period within an area of continuous habitat and an adjacent area of fragmented habitat. The average home range within the continuous habitat was 19.25 ha for males and 20.34 ha for females, with male and female pairs occupying a combined area of 23.15 ha. In contrast, the average home range in the fragmented habitat was 11.05 ha for males and 6.80 ha for females, with a combined home-range of male and female pairs being 11.60 ha. The average overlap of the home ranges of paired males and females was 85.9%, compared with approximately 11% for non-paired individuals, which suggests that mahogany gliders are socially monogamous. For a total of 46 nights on which gliders were considered to behave normally for the entire night, the average foraging distance was 1506 m (range 590–3420 m) with no significant difference between males and females in either the continuous or fragmented habitat. There was, however, a significant difference in the distance individuals travelled during different times of the year, with longer distances being travelled during late dry season/wet season and shorter distances during the early to mid dry season. Mahogany gliders also travelled further when there was a high availability of nectar and pollen than when there was lower availability. A total of 83 dens (tree hollows) were recorded for the mahogany glider, with the poplar gum, Eucalyptus platyphylla, forest red gum, Eucalyptus tereticornis, and Clarkson's bloodwood, Corymbia clarksoniana, being most used.

On the duration of larval and nymphal quiescence in male and female Ixodid ticks

1966 ◽  
Vol 57 (1) ◽  
pp. 101-106
Author(s):  
B. Feldman-Muhsam ◽  
H. V. Muhsam
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Ixodid Ticks ◽  
The Other ◽  
Male And Female ◽  
Significant Difference ◽  
Males And Females ◽  
The Difference

Differences between sexes in the duration of larval and nymphal quiescence in Ixodid ticks were investigated in Israel in the laboratory. In H. dromedarii Koch the larval quiescence of the male was, on average about 4 hours longer than that of the female, but in Rhipicephalus sanguineus (Latr.) no significant difference was found. In five species a statistically significant difference in the length of nymphal quiescence was found between males and females; in three of them, R. sanguineus, R. secundus Fel.-Muh. and H. dromedarii, that of the male exceeded that of the female by 1/2 to 1 1/2 days and in the other two, H. marginatum Koch and H. excavatum Koch, the opposite was found, the difference being 1·3 and 3·7 days, respectively.

Development and outcome of intrasexual contests in males and females of Chalcides viridanus (Fam. Scincidae) along breeding period

Behaviour ◽  
2019 ◽  
Vol 156 (13-14) ◽  
pp. 1349-1369
Author(s):  
P. Sánchez-Hernández ◽  
M. Molina-Borja
Keyword(s):  
Assessment Model ◽  
Breeding Period ◽  
Head Width ◽  
Tongue Flick ◽  
Self Assessment ◽  
Male And Female ◽  
Hind Limbs ◽  
Significant Difference ◽  
Males And Females ◽  
First Time

Abstract Morphological and behavioural traits influence contest development and outcome. We analysed morphological and behavioural traits in male and female staged contests of Chalcides viridanus along breeding time. There was no significant difference in any morphologic trait for winner and looser male contenders; larger hind-limbs and heads were significantly associated to winner females. ‘Approach’ was positively while ‘flee’ negatively associated to winner males. ‘Tongue-flick’ and ’approach’ were positively associated to female winners and ‘flee’ to losers. Contest intensity was higher in male than in female contests. For males it was higher in May than in March or April but for females in April and May than in March. Contest intensity was positively related to head width in loser males, suggesting fitting to a pure self-assessment model. For females there was no significant association. For the first time we have shown that skink female contests are as complex as those of males.

Sign in / Sign up

Export Citation Format

Share Document