Evaluation of E- cadherin and Hydrogen Peroxide in Skin of Patients with Vitiligo

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Marwa M Abdel-Rahim Abdallah ◽  
Mona Hussien Raafat Ahmed ◽  
Manal Louis Louka ◽  
Christine Kamal Thabet Gobrial ◽  
Alaa Abdel-Hakim Abdel-Magid
Keyword(s):  
Oxidative Stress ◽  
Skin Color ◽  
Case Control Study ◽  
Normal Skin ◽  
Police Academy ◽  
Group I ◽  
H2o2 Level ◽  
Cell To Cell Adhesion ◽  
Control Study ◽  
E Cadherin

Abstract Background Vitiligo is acquired depigmentary disorder characterized by destruction of the epidermal melanocytes leading to the loss of the skin color. Oxidative stress has a major role in the aetiopathogenesis and in melanocytic destruction due to its accumulation in the melanocytes and the hazardous effects to all compartments of the cell. Objective The aim of the work was to evaluate the level of E-cadherin and H2O2 level in vitiligo versus controls. Subjects This is a case control study which was carried out at Department of Dermatology, Venereology and Andrology,New Cairo Police Academy Hospital on 20 Subjects were divided into two groups, Group I included 10 patients having non segemental vitiligo. Group II included 10 non vitilignous controls were included in the study. Results We found that H2O2 level is increased in NSV patients when compared with healthy individuals. While E-cadherin level is significantly decreased in vitiligo skin compared to normal skin. Conclusion As compared to controls, increased H2O2 levels levels were suggestive of oxidative stress in patients of vitiligo in our study. From our results we can conclude that vitiligo is not a disease confined to melanocytes only, keratinocytes also showed certain pathological changes in vitiliginous lesions. As functional and structural units with melanocytes, keratinocytes in depigmented epidermis may constitute a different microenvironment compared to those in normally pigmented epidermis. These differences include obvious loss of cell to cell adhesion between keratinocytes and melanocytes and between keratinocytes and each other, which in turn may affect the pigmentary system of the skin.

TRANSFUSION INDUCED ALLERGIC REACTIONS AMONG THE PATIENTS OF APLASTIC ANAEMIA - A CASE CONTROL STUDY FROM BANGLADESH..

2019 ◽  
pp. 1-6
Author(s):  
Bepasha Naznin ◽  
Md. Ashraful Hoque* ◽  
Daanish Arefin Biswas ◽  
Tamanna Afroz ◽  
Farida Parvin ◽  
...  
Keyword(s):  
Allergic Reaction ◽  
Aplastic Anaemia ◽  
Case Control Study ◽  
Platelet Concentrate ◽  
Transfusion Reaction ◽  
Group I ◽  
Multiple Unit ◽  
Group Ii ◽  
Control Study ◽  
Transfusion Reactions

Background:Allergic transfusion reactions (ALTR) are very common complication of blood transfusion. Advances in transfusion medicine have significantly decreased the incidence of ALTR; however, ALTR continue to be burdensome for transfusion dependent patients. It increases their existing sufferings. Allergic reaction is more common in platelet concentrate transfusion because stored platelet concentrate supernatants (PCSNs) accumulate striking levels of biological response modifiers (BRMs) during storage. Objective: To determine the risk factors of allergic reactions in platelate concentrate transfusion. Method: It is a case control study enrolled a total of 64 diagnosed case of aplastic anaemia receiving transfusion of platelet concentrate at Department of Transfusion Medicine, BSMMU, Dhaka, from May 2015 to April 2016. Among them 32 case of aplastic anaemia having allergic reaction due to transfusion of platelet concentrate was considered as group I (case) and rest 32 patients not developed allergic transfusion reaction due to transfusion of platelet concentrate was considered as group II (control). Patients age belong to 5 - 50 years and both sex and also patients getting transfusion of plate late concentrate were enrolled in this study. Statistical analysis: Statistical analyses were carried out by using the Statistical Package for Social Sciences version 20.0 for Windows (SPSS Inc., Chicago, Illinois, USA). Chi-Square test, Odds ratio with 95% CI used to analyze the categorical variables, shown with cross tabulation. Student ttest used for continuous variables. Significant value of 'p' was decided to be at a level of 0.05 in two tailed tests. Result: The mean age was found 22.1±11.58 years in group I and 23.5±3.8 years in group II. Twenty four (75.0%) patients were male in group I and 17(53.1%) patients in group II. Almost twenty (62.5%) patients come from urban area in group I and 19(59.4%) in group II. In group I, thirty two (100.0%) patients had urticarialrash, 100.0% had itching, 37.5% had angioedema, 3.1% had cough, 3.1% had chest pain, 3.1% had respiratory distress, 3.1% had fever and 3.1% had vomiting. Almost twenty(62.5%) patients had tachycardia (>100 bpm) in group I and all patients had normal pulse in group II.Thirty two (100.0%) patients had normal blood pressure in both group. In group I, 31(96.9%) patients developed mild allergic reaction, 3.1% moderate allergic reaction. Majority (40.6%) patients belonged to age 16-30 years in present allergic transfusion reaction and 11(34.3%) in absent allergic transfusion reaction. Most (40.6%) of the patients was found blood group B in present allergic transfusion reaction and 11(34.4%) in absent allergic transfusion reaction. Multiple unit of PC transfusion increases 3.69 times risk to develop allergic transfusion reactions with 95% CI 0.99 to 14.44%.Platelet concentrate storage>3daysincreases 5.95timesrisktodevelopallergic transfusionreactionwith95%CI1.75to21.09%. Conclusion: Multiple unit (≥2) transfusion and Platelet concentrate storage >3 days were significantly (p<0.05) associated with allergic transfusion reactions but no significant association was found between allergic transfusion reactions with age and Blood group

NUTRITION, IMMUNITY, AND INFECTIONS: T LYMPHOCYTE SUBPOPULATIONS IN PROTEIN-ENERGY MALNUTRITION

PEDIATRICS ◽  
1995 ◽  
Vol 96 (1) ◽  
pp. 77-77
Author(s):  
H Ozkan ◽  
N Olgun ◽  
E Sasmaz
Keyword(s):  
T Lymphocytes ◽  
Case Control Study ◽  
Protein Energy Malnutrition ◽  
Control Group ◽  
Humoral And Cellular Immunity ◽  
Protein Energy ◽  
Group I ◽  
Suppressor T Lymphocytes ◽  
Degrees Of Severity ◽  
Control Study

This study was a case-control study of 44 children ages 3 to 24 months. The purpose of the study was to compare the humoral and cellular immunity of 29 patients (Group I) who were less than the 3rd percentile for weight by Turkish standards versus a control group of 15 patients (Group II) with weights between the 25th and 90th percentiles. The Group I patients were considered to have protein energy malnutrition (PEM) with various degrees of severity based on a Turkish classification method established by Dogramaci and Wray in 1958. None of the Group I patients had frank kwashiorkor, but 19 had bronchopneumonia, six had gastroenteritis, and four had both forms of infection at the time studies were done. Detailed immunologic evaluation was carried out on all the subjects including IgG, IgM, IgA, C3, mature T lymphocytes (CD3+), helper/inducer T Lymphocytes (CD4+) and suppressor/cytotoxic T lymphocytes (CD8+). The authors chose to study patients with PEM and infections because the immune response is more likely to be suppressed at this time. In the PEM group all of the immunoglobulins (IgG, IgM, IgA) were significantly elevated over the controls level (P &lt; .01). This hyperimmunoglobulinemia state has been previously reported and could be secondary to reduced suppressor T lymphocytes (C8+). C3 complement levels were also significantly lower (P &lt; .01) than controls, which have been previously noted. These low C3 levels could be secondary to decreased production from the liver or increased utilization with an intercurrent infection. In evaluating the lymphocyte series CD3+, CD4+, and CD8+ were all significantly reduced while CD4/CD8 levels were normal.

scholarly journals Methamphetamine’s effects on oxidative stress markers may continue after detoxification: a case–control study

2019 ◽  
Vol 29 (3) ◽  
pp. 361-367
Author(s):  
Yunus Hacimusalar ◽  
Ozgul Karaaslan ◽  
Ceylan Bal ◽  
Derya Kocer ◽  
Gamze Gok ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Case Control Study ◽  
Case Control ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Control Study

Threshold values of antibodies to estrogen, progesteron and benzo [a] pyrene as a risk factor for the development of endometriosis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Natalia V. Artymuk ◽  
Vitaliy O. Chervov ◽  
Larissa N. Danilova ◽  
Elena G. Polenok ◽  
Olga Zotova
Keyword(s):  
Risk Factor ◽  
Roc Analysis ◽  
Case Control Study ◽  
Case Control ◽  
Threshold Values ◽  
Retrospective Case ◽  
Group I ◽  
Group Ii ◽  
Conventional Unit ◽  
Control Study

Abstract Objectives The objective of the study was to determine the level of antibodies (AB) of Ig classes A and G to estradiol (E2), progesterone (P) and benzo [a] pyrene (Bp) in patients with endometriosis of various severity and estimate their threshold values as a risk factor for the development of endometriosis. Methods A retrospective case–control study was performed. The study involved 200 women. Group I: women with endometriosis (n=100), Group II: patients with tubal-peritoneal infertility (n=100). All patients underwent immunological studies of blood serum; and the levels of steroid hormones (P, E2), antibodies to them and Bp were determined. A ROC analysis was carried out to identify threshold values of antibodies levels. Results Women with endometriosis were found to have statistically significantly higher levels of antibodies IgA and IgG to E2, P and benzo [a] pyrene compared to women of Group II. The threshold levels of IgA-Bp, IgA-E2 and IgA-P are >5 CU (conventional unit), IgG-Bp, IgG-E2>9 CU and IgG-P>8 CU. The level of IgG-P in patients with severe forms of endometriosis is statistically significantly higher than in minor forms of the disease. In case of severe forms, there is a tendency to increasing other classes of antibodies. Conclusions Patients with endometriosis usually have a higher level of IgA and IgG to Bp, E2, P. Their threshold values, which are risk factors for the development of the disease, are estimated.

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