scholarly journals Genome sequence resource of Botryosphaeria dothidea CK16, a fungal pathogen causing Chinese hickory trunk canker disease

Plant Disease ◽  
2021 ◽  
Author(s):  
Yuxin Rao ◽  
Li Mei ◽  
Liqin Zhang ◽  
Hong Jiang ◽  
Liangjin Ma ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Fungal Pathogen ◽  
Woody Plants ◽  
Host Plants ◽  
Botryosphaeria Dothidea ◽  
Canker Disease ◽  
Host Pathogen Interaction ◽  
Carya Cathayensis ◽  
High Quality Genome ◽  
Trunk Canker

Botryosphaeria dothidea is a latent fungal pathogen that causes cankers or diebacks on a variety of host woody plants worldwide. The symptomatic necrosis on the host plants can be triggered by the abiotic stress, such as drought and soil acidification. Here we report a high-quality genome assembly and announcement of the B. dothidea strain CK16 (CGMCC 19654) which causes trunk canker disease on Carya cathayensis in China. The genome sequence of strain CK16 will be useful for studying the evolution, host adaption and pathogenicity of B. dothidea, which will be beneficial for a better understanding of the mechanisms of host-pathogen interaction during the endophytic period.

scholarly journals Genome Assembly and Annotation of Botryosphaeria dothidea sdau11-99, a Latent Pathogen of Apple Fruit Ring Rot in China

Plant Disease ◽  
2020 ◽  
Author(s):  
Chengming Yu ◽  
Yufei Diao ◽  
Quan Lu ◽  
Jiaping Zhao ◽  
Shengnan Cui ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Genome Assembly ◽  
Fungal Pathogen ◽  
Woody Plants ◽  
Reference Genome ◽  
Apple Fruit ◽  
Botryosphaeria Dothidea ◽  
High Quality ◽  
Ring Rot ◽  
Wide Range

Botryosphaeria dothidea is a latent and important fungal pathogen on a wide range of woody plants. Fruit ring rot caused by B. dothidea is a major disease in China on apple. This study establishes a high quality, nearly complete and well annotated genome sequence of B. dothidea strain sdau11-99. The findings of this research provide a reference genome resource for further research on the apple fruit ring rot pathogen on apple and other hosts.

scholarly journals Genome Sequence Resource of Magnaporthe oryzae Laboratory Strain 2539

2020 ◽  
Vol 33 (8) ◽  
pp. 1029-1031
Author(s):  
Meilian Chen ◽  
Baohua Wang ◽  
Guodong Lu ◽  
Zhenhui Zhong ◽  
Zonghua Wang
Keyword(s):  
Genome Sequence ◽  
Magnaporthe Oryzae ◽  
Genome Assembly ◽  
Laboratory Strain ◽  
Model System ◽  
Blast Disease ◽  
High Quality ◽  
Host Pathogen Interaction ◽  
Host Pathogen ◽  
High Quality Genome

Magnaporthe oryzae causes blast disease on more than 50 species of monocot plants, including important crops such as rice, millet, and most recently wheat. Additionally, it is an important model system for studying host-pathogen interaction. Here, we report a high-quality genome assembly and annotation of a laboratory strain 2539 of M. oryzae, which is a widely used progeny of a rice-infecting isolate and a grass-infecting isolate. The genome sequence of strain 2539 will be useful for studying the evolution, host adaption, and pathogenicity of M. oryzae, which will be beneficial for a better understanding of the mechanisms of host-pathogen interaction.

scholarly journals q-LAMP Assays for the Detection of Botryosphaeria dothidea Causing Chinese Hickory Canker in Trunk, Water, and Air Samples

Plant Disease ◽  
2019 ◽  
Vol 103 (12) ◽  
pp. 3142-3149
Author(s):  
Q. W. Wang ◽  
C. Q. Zhang
Keyword(s):  
Limit Of Detection ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Botryosphaeria Dothidea ◽  
Canker Disease ◽  
Air Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Optimum Reaction ◽  
Epidemiological Characteristics ◽  
Trunk Canker

Trunk canker disease caused by Botryosphaeria dothidea with a prolonged latent infection phase poses a serious threat to Chinese hickory production. To further understand the epidemiological characteristics and develop reasonable management techniques, a quantitative loop-mediated isothermal amplification (q-LAMP) assay was developed to quantitatively monitor B. dothidea in hickory plants, water, and air samples. Specific primers were designed based on the different sites of the β-tubulin sequence between B. dothidea and other fungi commonly found on Chinese hickory. At the optimum reaction temperature of 65.9°C, this loop-mediated isothermal amplification (LAMP) assay can specifically distinguish B. dothidea from other tested fungi. The limit of detection of LAMP assays for B. dothidea was 0.001 ng/µl of pure genomic DNA and 10 spores per 1 ml of water. The q-LAMP assay enables rapid detection of B. dothidea within 60 min in hickory trunk, water in hickory forests, and spores captured on tapes. These results provide a powerful and convenient tool for monitoring B. dothidea, which could be applied widely in epidemiology, forecast, and management of tree canker disease.

scholarly journals Genome sequence resource for the plant pathogen Sclerotinia sclerotiorum WH6 isolated in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Xiong Zhang ◽  
Xiaohui Cheng ◽  
Lijiang Liu ◽  
Shengyi Liu
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Genome Sequence ◽  
Fungal Pathogen ◽  
Sclerotinia Stem Rot ◽  
High Quality ◽  
Total Size ◽  
Host Interactions ◽  
A Genome ◽  
The World ◽  
High Quality Genome

Sclerotinia sclerotiorum is a notorious fungal pathogen that causes sclerotinia stem rot (SSR) on many important crops in China and worldwide. Here, we present a high- quality genome assembly of S. sclerotiorum strain WH6 using the PacBio SMRT cell platform. The assembled genome has a total size of 38.96 Mbp, with a contig N50 length of 1.90 Mbp, and encodes 10,512 predicted coding genes, including 685 secreted proteins and 65 effector candidates. This is the the first report of a genome sequence from China. The WH6 genome sequence provides a valuable resource for facilitating our understanding of S. sclerotiorum-host interactions and SSR control in China and the world.

Diversity of Botryosphaeriaceae species associated with Chinese hickory tree (Carya cathayensis) trunk cankers

Plant Disease ◽  
2021 ◽  
Author(s):  
C. J. Zhuang ◽  
Q. W. Wang ◽  
Q. Q. Wu ◽  
Z. L. Qiu ◽  
B. C. Xu ◽  
...  
Keyword(s):  
Spore Germination ◽  
Fungal Pathogen ◽  
Mycelial Growth ◽  
Optimum Temperature ◽  
Botryosphaeria Dothidea ◽  
Pathogen Diversity ◽  
New Information ◽  
Different Temperatures ◽  
Carya Cathayensis ◽  
Botryosphaeriaceae Species

Tree trunk cankers (TRC) represent serious fungal diseases that pose significant threats to Chinese hickory trees (Carya cathayensis). To characterize the pathogen diversity associated with, diseased tissues were collected between 2016 and 2018 from the primary Chinese hickory plantation regions. A total of 97 cultures were isolated from trees in six towns (Longgang, Qingliangfeng, Changhua, Tuankou, Taiyang Town, and Lin’an urban area) within the Linan district, where 60% of Chinese hickory tree yields originate. The isolated cultures caused cankers on Chinese hickory tree branches, but infections did not occur on fruits or leaves under tested conditions. Combined morphological observations and phylogenetic analysis of multiple genes (ITS, β-tublin, and EF) indicated that five Botryosphaeriaceae species were recovered, including 89 isolates of Botryosphaeria dothidea, four isolates of B. fabicerciana, one isolate of B. qingyuanensis, one isolate of B. corticis, and two isolates of Lasiodiplodia theobromae. B. dothidea was the most prevalent, and this is the first report of B. corticis, B. qingyuanensis, and L. theobromae infections in Chinese hickory trees. We investigated the mycelial growth, spore germination, and pathogenicity of these species at different temperatures. L. theobromae grew the fastest and B. cortices grew the slowest on PDA. The optimum temperature of spore germination for all species was 30°C. L. theobromae was the most virulent species, followed by B. dothidea and B. qingyuanensis, then B. fabicerciana, and finally B. cortices. These new insights into fungal pathogen diversity provide critical new information to understand and manage TRC of Chinese hickory.

scholarly journals High-quality genome assembly and annotation resource of Botryosphaeria dothidea strain BDLA16-7, causing trunk canker disease on Chinese hickory

Plant Disease ◽  
2021 ◽  
Author(s):  
Jiandong Bao ◽  
Q. Q. Wu ◽  
Jianqin Huang ◽  
Chuan-Qing Zhang
Keyword(s):  
Genome Assembly ◽  
Gene Clusters ◽  
Botryosphaeria Dothidea ◽  
Cytochrome P450 Enzymes ◽  
Protein Coding ◽  
Canker Disease ◽  
Host Interaction ◽  
Oxford Nanopore ◽  
Infection Mechanisms ◽  
High Quality Genome

Botryosphaeria dothidea is a latent pathogen with global importance to woody plant health, which causes serious tree trunk cankers on Chinese hickory. To date, only one Illumina short-read-based genome assembly of strain CK16 is available for host Chinese hickory. To address this problem, we reported a near telomere-to-telomere genome assembly of strain BDLA16-7 (46.05 Mb, N50 3.87 Mb) using Oxford Nanopore Sequencing Technology. Our genome assembly was consisted of 15 contigs, of which, 3 were assembled into chromosomal level and the maximum contig length was 6.19 Mb. The assembly contained 7.96% repeats and 12,815 protein-coding genes (10,274 genes were functional annotated). We also identified 3,642 pathogen-host interaction (PHI) genes, 250 carbohydrateactive enzymes (CAZymes), 252 cytochrome P450 enzymes (CYPs), 752 putative secreted proteins and 63 secondary metabolite biosynthesis gene clusters (SMBGCs). The BUSCO completeness of genome assembly and predicted genes was 99.34% and 97.50%, respectively, at fungal level (n=758). The almost chromosomal-level and well-annotated genome assembly will provide a valuable genetic resource for understanding of the infection mechanisms of B. dothidea in future.

scholarly journals The Genome Sequence of the Fungal Pathogen Fusarium virguliforme That Causes Sudden Death Syndrome in Soybean

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e81832 ◽  
Author(s):  
Subodh K. Srivastava ◽  
Xiaoqiu Huang ◽  
Hargeet K. Brar ◽  
Ahmad M. Fakhoury ◽  
Burton H. Bluhm ◽  
...  
Keyword(s):  
Sudden Death ◽  
Genome Sequence ◽  
Fungal Pathogen ◽  
Sudden Death Syndrome ◽  
Fusarium Virguliforme

Complete Genome Sequence of a Novel Ourmia-like Mycovirus Infecting the Phytopathogenic Fungus Botryosphaeria Dothidea

2021 ◽  
Author(s):  
Liying Sun ◽  
Ziqian Lian ◽  
Subha Das ◽  
Jingxian Luo ◽  
Ida Bagus Andika
Keyword(s):  
Amino Acid ◽  
Genome Sequence ◽  
Amino Acid Sequences ◽  
Shanxi Province ◽  
Phytopathogenic Fungus ◽  
Botryosphaeria Dothidea ◽  
Reading Frame ◽  
Ring Rot ◽  
Ssrna Virus ◽  
The Family

Abstract In this study, we describe the full-length genome sequence of a novel ourmia-like mycovirus, tentatively designated Botryosphaeria dothidea ourmia-like virus 1 (BdOLV1), isolated from the phytopathogenic fungus, Botryosphaeria dothidea strain P8, associated with apple ring rot in Shanxi province, China. The complete BdOLV1 genome is comprised of 2797 nucleotides, a positive-sense (+) single-stranded RNA (ssRNA) with a single open reading frame (ORF). The ORF putatively encodes a 642-amino acid polypeptide with conserved RNA-dependent RNA polymerase (RdRp) motifs, related to viruses of the family Botourmiaviridae. Phylogenetic analysis based on the RdRp amino acid sequences showed that BdOLV1 is grouped with oomycete-infecting unclassified viruses closely related to the genus Botoulivirus in Botourmiaviridae. This is the first report of a novel (+)ssRNA virus in B. dothidea related to the genus Botoulivirus in the family Botourmiaviridae.

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