Infection Caused by Phaeomoniella chlamydospora Associated with Esca-like Symptoms in Grapevine in Chile

Trunk diseases such as esca have been recognized as an economically important problem of grapevine worldwide. A study was conducted to characterize the distribution of Phaeomoniella chlamydospora in Chile. A field survey of young and mature grapevines from 67 vineyards located along a 1,315-km north-south axis demonstrated that P. chlamydospora was present in 94.9% of the grapevine samples showing the black-wood streaking symptom (BWS) but not the characteristic foliar symptoms of esca. Phylogenetic analysis of the ribosomal DNA internal transcribed spacer (ITS) combined with β-tubulin (BT) genes grouped Chilean isolates together with reference isolates from South Africa and the United States, whereas Spanish isolates were clustered separately. Chilean isolates differed by only 2 to 3 bp for BT and ITS, respectively. Conidia germinated at 5 to 35°C, with an optimal temperature of 25 to 30°C. Isolates were pathogenic, and Koch's postulates were fulfilled in separate sets of inoculations of axenic plantlets, cuttings, 2-year-old plants, spurs, and shoots of V. vinifera. This study showed that P. chlamydospora was associated consistently with BWS and no other apparent symptom in young and mature grapevines, including nursery plants, in Chile. Inoculum was absent from the soil, grapevine pruning debris, sap samples, and herbaceous weeds, which is in contrast to past studies. At this time, Vitis spp. are the only known hosts of P. chlamydospora in Chile.

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Fomitiporia punicata and Phaeoacremonium minimum associated with Esca complex of grapevine in China

Phytopathology Research ◽

10.1186/s42483-021-00087-w ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Qingtong Ye ◽

Jingyi Jia ◽

Ishara S. Manawasinghe ◽

Xinghong Li ◽

Wei Zhang ◽

...

Keyword(s):

Field Survey ◽

Phylogenetic Analyses ◽

First Record ◽

Morphological Characters ◽

Yield Losses ◽

Detailed Report ◽

Foliar Symptoms ◽

Esca Disease ◽

Trunk Diseases ◽

Leaf Symptoms

AbstractThe Esca disease complex includes some of the most important trunk diseases of grapevines (Vitis spp.) and causes serious yield losses in grape production worldwide. However, there has been no detailed study on its presence and associated pathogens in China. During 2017–2019, a preliminary field survey was conducted in eight vineyards in Hebei and Ningxia provinces, China when unusual foliar symptoms were observed. Symptoms were distinct tiger striped leaves, which are typical of grapevine leaf stripe disease (GLSD), one of the most common diseases in the Esca complex. Tiger-stripe leaf symptoms were found in four vineyards, and incidence was cultivar dependent varying with vineyard and year. A total of 266 fungal isolates were obtained from wood tissues of grapevines with typical foliar symptoms of GLSD. Based on morphological characters and multigene-combined phylogenetic analyses, the Ascomycete Phaeoacremonium minimum, one of the pathogens associated with Esca complex was identified. The basidiomycete Fomitiporia punicata, which has never been reported infecting grapevine, was also identified and found to be associated with wood rot in grapevine. The remaining isolates included some known wood pathogens, such as Neofusicoccum species and Diaporthe species. Koch’s postulates were performed in the greenhouse, confirming that both F. punicata and P. minimum caused leaf interveinal chlorosis and necrosis that resembled the GLSD symptoms of the Esca complex observed in the field. The present study provides the first detailed report of the Esca complex in China. In addition, this is the first record of F. punicata associated with Esca complex of grapevine worldwide. The results of this study provide new insights into the knowledge of the Esca complex.

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First Report of Phytophthora megasperma and Pythium irregulare As Olive Tree Root Pathogens

Plant Disease ◽

10.1094/pdis.1997.81.10.1216b ◽

1997 ◽

Vol 81 (10) ◽

pp. 1216-1216 ◽

Cited By ~ 4

Author(s):

M. E. Sánchez-Hernández ◽

A. Ruiz-Dávila ◽

A. Trapero-Casas

Keyword(s):

Root Rot ◽

Olive Tree ◽

The United States ◽

Damping Off ◽

Phytophthora Megasperma ◽

Root Rots ◽

Foliar Symptoms ◽

Root Pathogens ◽

Root Necrosis ◽

Olive Trees

Several species of the genus Phytophthora are associated with root rot and trunk cankers in olive trees (Olea europaea L.). Among them, Phytophthora megasperma has been cited as being associated with olive root rots in Greece (1). Unidentified species of Pythium and Phytophthora have also been associated with olive tree root rots in the United States. However, the status of P. megasperma and Pythium spp. as olive tree root pathogens has remained unclear. Following a 5-year period of severe drought in southern Spain, autumn-winter rainfall rates in 1996 to 1997 steadily increased in both quantity and frequency. Under these unusually wet conditions, olive trees remained waterlogged for several months. During this period, we observed foliar wilting, dieback, and death of young trees, and later found extensive root necrosis. In 46 of 49 affected plantations surveyed, P. megasperma was consistently isolated from the rotted rootlets, particularly in young (<1- to 10-year-old trees) plantations. This fungus was not detected on plant material affected by damping-off from several Spanish olive tree nurseries. The opposite situation occurred with P. irregulare. This species was not associated with rotted rootlets in the field. In contrast, it was consistently isolated from necrotic rootlets from young olive plants affected by damping-off. These plants were grown in a sand-lime-peat soil mixture under greenhouse conditions and showed foliar wilting and extensive necrosis of the root systems. Pathogenicity tests were conducted with several isolates of P. megasperma and P. irregulare on 6-month-old rooted cuttings of olive, under both weekly watering and waterlogged conditions. Under waterlogged conditions, both fungal species produced extensive root necrosis 2 weeks after inoculation that resulted in wilting of the aerial parts and rapid plant death. Waterlogged control plants remained without foliar symptoms but a low degree of root necrosis was recorded. In addition, under weekly watering conditions, plants inoculated with either species showed some degree of root rot but foliar symptoms were not evident. No differences in pathogenicity were observed within the Phytophthora or Pythium isolates. Reference: (1) H. Kouyeas and A. Chitzanidis. Ann. Inst. Phytopathol. Benaki 8:175, 1968.

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Development of PCR Assays for the Identification of Species and Pathotypes of Elsinoë Causing Scab on Citrus

Plant Disease ◽

10.1094/pdis-91-7-0865 ◽

2007 ◽

Vol 91 (7) ◽

pp. 865-870 ◽

Cited By ~ 16

Author(s):

J. W. Hyun ◽

N. A. Peres ◽

S.-Y. Yi ◽

L. W. Timmer ◽

K. S. Kim ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Sweet Orange ◽

Random Amplified Polymorphic Dna ◽

Its Region ◽

The United States ◽

Specific Primer ◽

Identification Of Species ◽

Orange Fruit ◽

Pcr Assays ◽

Primer Sets

Two scab pathogens of citrus, Elsinoë fawcettii and E. australis, cause citrus scab and sweet orange scab, respectively, and pathotypes of each species have been described. The two species cannot be readily distinguished by morphological or cultural characteristics and can be distinguished only by host range and the sequence of the internal transcribed spacer (ITS) region. In this study, random amplified polymorphic DNA (RAPD) assays clearly distinguished E. fawcettii and E. australis, and the sweet orange and natsudaidai pathotypes within E. australis also could be differentiated. We developed specific primer sets, Efaw-1 for E. fawcettii; Eaut-1, Eaut-2, Eaut-3, and Eaut-4 for E. australis; and EaNat-1 and EaNat-2 for the natsudaidai pathotype within E. australis using RAPD products unique to each species or pathotype. Other primer sets, Efaw-2 and Eaut-5, which were specific for E. fawcettii and E. australis, respectively, were designed from previously determined ITS sequences. The Efaw-1 and Efaw-2 primer sets successfully identified E. fawcettii isolates from Korea, Australia, and the United States (Florida) and the Eaut-1 to Eaut-5 primer sets identified both the sweet orange pathotype isolates of E. australis from Argentina and the natsudaidai pathotype isolates from Korea. The EaNat-1 and EaNat-2 primer sets were specific for isolates of the natsudaidai pathotype. The Efaw-1 and Efaw-2 primer sets successfully detected E. fawcettii from lesions on diseased leaves and fruit from Korea and primer pairs Eaut-1, Eaut-2, Eaut-3, Eaut-4, and Eaut-5 detected E. australis from lesions on sweet orange fruit from Brazil.

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First Report of Orange Rust of Sugarcane Caused by Puccinia kuehnii in Colombia

Plant Disease ◽

10.1094/pdis-05-11-0406 ◽

2012 ◽

Vol 96 (1) ◽

pp. 143-143 ◽

Cited By ~ 6

Author(s):

M. Cadavid ◽

J. C. Ángel ◽

J. I. Victoria

Keyword(s):

Internal Transcribed Spacer ◽

Pcr Primers ◽

The United States ◽

Optical Microscope ◽

First Report ◽

5.8S Rdna ◽

Specific Pcr ◽

Plant Pathol ◽

Species Specific ◽

New Cultivars

Symptoms of sugarcane orange rust were first observed in July 2010 on sugarcane (interspecific hybrid of Saccharum L. species) cv. CC 01-1884 planted in the La Cabaña Sugar Mill, Puerto Tejada, Colombia. Morphological features of uredinial lesions and urediniospores inspected with an optical microscope and scanning electron microscopy were distinct from common rust of sugarcane caused by Puccinia melanocephala Syd. & P. Syd., revealing spores identical morphologically to those described for the fungus P. kuehnii (Kruger) E. Butler, causal agent of sugarcane orange rust (1,3). Uredinial lesions were orange and distinctly lighter in color than pustules of P. melanocephala. Urediniospores were orange to light cinnamon brown, mostly ovoid to pyriform, variable in size (27.3 to 39.2 × 16.7 to 21.2 μm), with pronounced apical wall and moderately echinulate with spines evenly distributed. Paraphyses, telia, and teliospores were not observed. Species-specific PCR primers designed from the internal transcribed spacer (ITS)1, ITS2, and 5.8S rDNA regions of P. melanocephala and P. kuehnii were used to differentiate the two species (2). The primers Pm1-F and Pm1-R amplified a 480-bp product from P. melanocepahala DNA in leaf samples with symptoms of common rust. By contrast, the primers Pk1-F and Pk1-R generated a 527-bp product from presumed P. kuehnii DNA in leaf samples with signs of orange rust, confirming the identity as P. kuehnii. The Centro de Investigación de la Caña de Azúcar de Colombia (Cenicaña) started a survey of different cultivars in nurseries and experimental and commercial fields in the Cauca River Valley and collected leaf samples for additional analyses. Experimental cvs. CC 01-1884, CC 01-1866, and CC 01-1305 were found to be highly susceptible to orange rust and were eliminated from regional trials, whereas commercial cvs. CC 85-92 and CC 84-75, the most widely grown cultivars, were resistant. With the discovery of orange rust of sugarcane in Colombia, Cenicaña has incorporated orange rust resistance in the selection and development of new cultivars. To our knowledge, this is the first report of P. kuehnii on sugarcane in Colombia. Orange rust has also been reported from the United States, Cuba, Mexico, Guatemala, Nicaragua, El Salvador, Costa Rica, Panama, Ecuador, and Brazil. References: (1) J. C. Comstock et al. Plant Dis. 92:175, 2008. (2) N. C. Glynn et al. Plant Pathol. 59:703, 2010. (3) E. V. Virtudazo et al. Mycoscience 42:167, 2001.

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First Report of Musk Thistle Rust (Puccinia carduorum) in California and Nevada

Plant Disease ◽

10.1094/pdis.2002.86.7.814b ◽

2002 ◽

Vol 86 (7) ◽

pp. 814-814 ◽

Cited By ~ 5

Author(s):

D. M. Woods ◽

M. J. Pitcairn ◽

D. G. Luster ◽

W. L. Bruckart

Keyword(s):

Ribosomal Rna ◽

Dna Sequences ◽

Internal Transcribed Spacer ◽

East Coast ◽

Rust Fungus ◽

The United States ◽

Rust Disease ◽

Carduus Nutans ◽

Its1 And Its2 ◽

Spacer Dna

Musk thistle, Carduus nutans L., is an introduced weed of pastures, rangelands, and natural areas in much of North America. Puccinia carduorum Jacky, an autoecious rust fungus from Turkey, has been evaluated for biological control of musk thistle since 1978, including a field study near Blacksburg, VA, from 1987 to 1990. After release of the fungus in Virginia, rusted musk thistle was found in eight eastern states by 1992, in Missouri by 1994 (1), and in Oklahoma by 1997 (2). A rust disease was discovered on musk thistle near Mt. Shasta, CA, on 22 September 1998, and near Mogul, NV, on 12 August 1999. The pathogen was identified as P. carduorum on the basis of pathogenicity on musk thistle and urediniospore morphology (ovate spores, 21 μm diameter, three germ pores equatorial in location, and echinulations over the upper two-thirds to three-quarters of urediniospores). Ribosomal RNA internal transcribed spacer DNA sequences (ITS1 and ITS2) were identical to those from the isolate obtained after the field release in Virginia, verifying that the California isolate is P. carduorum. The initial California infestation was observed on a few plants late in the season, and by September 2000, nearly 100% of plants were infected. The occurrence of P. carduorum in California is apparently the result of natural, unaided spread of the fungus on musk thistle from the East Coast of the United States. References: (1) A. B. A. M. Baudoin and W. L. Bruckart. Plant Dis. 80:1193, 1996. (2) L. J. Littlefield et al. Plant Dis. 82:832, 1998.

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Sarcocystis sp. parasites in the Mexican Great-tailed Grackle (Quiscalus mexicanus), Bronzed Cowbird (Molothrus aeneus), and Stripe-headed Sparrow (Aimophila ruficauda)

Veterinaria México OA ◽

10.21753/vmoa.1.2.336 ◽

2014 ◽

Vol 1 (2) ◽

Author(s):

Félix Domingo Sánchez-Godoy ◽

Fernando Chávez-Maya ◽

Adriana Méndez-Bernal ◽

Gary García-Espinosa ◽

Cristina Guerrero-Molina ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Internal Transcribed Spacer ◽

Sequence Similarity ◽

Bird Species ◽

The United States ◽

Histopathological Analysis ◽

Biological Studies ◽

Pcr Rflp ◽

Molothrus Aeneus ◽

Specific Fragment

The objective of this study was to describe the morphological and ultraestructural characteristics, the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) results, the sequences and the phylogenetic analysis of a specific fragment of internal transcribed spacer 1 (ITS-1), amplified using the 25/396 primers, of the Sarcocystis sp. parasites identified in the muscles of wild great-tailed grackles, bronzed cowbirds, and stripe-headed sparrows in Mexico. Fifteen birds with sarcocystosis in their skeletal muscles were studied: 7 great-tailed grackles (Quiscalus mexicanus), 6 bronzed cowbirds (Molothrus aeneus), and 2 stripe-headed sparrows (Aimophila ruficauda). Histopathological analysis revealed thin-walled mature parasite cysts. Ultrastructurally, the cyst wall consisted of a granular layer with villar protrusions and numerous microtubules. The bradyzoites measured 4.1 × 1.6 µm, and micronemes appeared in the anterior third of the conoid. For molecular identification, PCR-RFLP was performed using sequences of a specific fragment of internal transcribed spacer 1 (ITS-1) using the primers 25/396 and Hinf I. Hind III did not cut this fragment. The sequencing results indicated a 100% similarity among the Sarcocystis parasites from the three bird species, and a BLAST search revealed 96% sequence similarity with S. neurona. The phylogenetic analysis shows that the sequences studied are topologically distant to those sequences reported for S. neurona in the United States and in South America and are not related to any group previously reported. Although our morphological and molecular analysis data provide strong evidence that S. neurona uses these bird species as intermediate hosts, future molecular studies with additional DNA fragments, combined with biological studies, will ultimately allow us to convincingly identify these parasites. This is the first report of a Sarcocystis sp. parasite in wild birds in Mexico that may be S. neurona.

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Field Response of Glyphosate-Tolerant Soybean to Herbicides and Sudden Death Syndrome

Plant Disease ◽

10.1094/pdis.2001.85.7.773 ◽

2001 ◽

Vol 85 (7) ◽

pp. 773-779 ◽

Cited By ~ 45

Author(s):

S. Sanogo ◽

X. B. Yang ◽

P. Lundeen

Keyword(s):

Sudden Death ◽

Field Experiments ◽

Foliar Application ◽

The United States ◽

Sudden Death Syndrome ◽

North Central ◽

The North ◽

Foliar Symptoms ◽

Field Response ◽

Soybean Plants

Three-year field experiments were conducted to assess the development of sudden death syndrome (caused by Fusarium solani f. sp. glycines) in three soybean cultivars, tolerant (P9344 and A3071) and nontolerant (BSR101), to glyphosate following foliar application of four herbicides (acifluorfen, glyphosate, imazethapyr, and lactofen) commonly applied to soybeans in the north-central region of the United States. Cultivar A3071 is resistant to sudden death syndrome, whereas cultivars P9344 and BSR101 are susceptible to this disease. There was no statistically significant cultivar-herbicide interaction with respect to the severity of foliar symptoms of the disease and the frequency of isolation of F. solani f. sp. glycines from roots of soybean plants. Across all herbicide treatments, the level of sudden death syndrome was lower in the disease-resistant cultivar than in the susceptible ones. There was an increase in the disease levels under application of acifluorfen, glyphosate, and imazethapyr compared with nontreated or lactofen-treated plants. The results obtained indicate that the response of glyphosate-tolerant soybeans to sudden death syndrome is not different from the response of conventional soybeans to this disease following application of the selected herbicides, and the resistance of soybean to sudden death syndrome was not changed with application of glyphosate.

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Detection of Grapevine Fungal Trunk Pathogens on Pruning Shears and Evaluation of Their Potential for Spread of Infection

Plant Disease ◽

10.1094/pdis-12-14-1283-re ◽

2015 ◽

Vol 99 (7) ◽

pp. 976-981 ◽

Cited By ~ 12

Author(s):

C. Agustí-Brisach ◽

M. León ◽

J. García-Jiménez ◽

J. Armengol

Keyword(s):

Simultaneous Detection ◽

Nested Polymerase Chain Reaction ◽

Fungal Isolation ◽

Diplodia Seriata ◽

Phaeomoniella Chlamydospora ◽

Eutypa Lata ◽

Grapevine Trunk Diseases ◽

Liquid Samples ◽

Trunk Diseases ◽

Spread Of Infection

Four vineyards visibly affected by trunk diseases were surveyed at pruning time in 2012 and 2013 in Spain, to determine whether pruning tools are capable of spreading grapevine trunk diseases from vine to vine. In each vineyard, pruning shears were regularly rinsed with sterile water, collecting liquid samples for analysis. Molecular detection of grapevine fungal trunk pathogens (GFTPs) was performed by nested polymerase chain reaction using specific primers to detect Botryosphaeriaceae spp. Eutypa lata, Cadophora luteo-olivacea, Phaeoacremonium spp., and Phaeomoniella chlamydospora. All of these GFTPs, with the exception of E. lata, were detected in samples from the four vineyards, C. luteo-olivacea and Phaeoacremonium spp. being the most prevalent. Co-occurrence of two, three, or four different GFTPs from the same sample were found, the simultaneous detection of C. luteo-olivacea and Phaeoacremonium spp. being the most prevalent. In addition, fungal isolation from liquid samples in semiselective culture medium for C. luteo-olivacea, Phaeoacremonium spp., and P. chlamydospora was also performed but only C. luteo-olivacea was recovered from samples collected in three of four vineyards evaluated. Pruning shears artificially infested with suspensions of conidia or mycelial fragments of C. luteo-olivacea, Diplodia seriata, E. lata, Phaeoacremonium aleophilum, and Phaeomoniella chlamydospora were used to prune 1-year-old grapevine cuttings of ‘110 Richter’ rootstock. Successful fungal reisolation from the cuttings 4 months after pruning confirmed that infested pruning shears were able to infect them through pruning wounds. These results improve knowledge about the epidemiology of GFTPs and demonstrate the potential of inoculum present on pruning shears to infect grapevines.

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Colonization of Vitis spp. Wood by sGFP-Transformed Phaeomoniella chlamydospora, a Tracheomycotic Fungus Involved in Esca Disease

Phytopathology ◽

10.1094/phyto-06-11-0165 ◽

2012 ◽

Vol 102 (3) ◽

pp. 290-297 ◽

Cited By ~ 14

Author(s):

Lucia Landi ◽

Sergio Murolo ◽

Gianfranco Romanazzi

Keyword(s):

Fluorescent Protein ◽

Incubation Temperature ◽

Quantitative Polymerase Chain Reaction ◽

Fluorescence Emission ◽

Epifluorescence Microscopy ◽

Hygromycin B ◽

Conidial Suspension ◽

Phaeomoniella Chlamydospora ◽

Esca Disease ◽

Vitis Spp

To evaluate wood colonization and interactions with Vitis spp. of Phaeomoniella chlamydospora, a fungal agent involved in Esca disease, isolate CBS 229.95 was transformed using a pCT74 construct which contained the genetic markers for synthetic green fluorescent protein (sGFP) and hygromycin B phosphotransferase. Nine stable P. chlamydospora fungal transformants (Pch-sGFP lines) were obtained using polyethylene-glycol-mediated transformation of protoplasts. These were characterized for sgfp and hygromycin B phosphotransferase (hph) genome insertions and for sGFP fluorescence emission, using quantitative polymerase chain reaction and fluorimetric systems, respectively. No correlation was observed between sgfp copy number genome insertion and sGFP fluorescence expression. Cuttings of Vitis vinifera ‘Montepulciano’, ‘Verdicchio’, ‘Sangiovese’, ‘Biancame’, and ‘Cabernet Sauvignon’; and the grapevine rootstocks ‘Kober 5BB’, ‘SO4’, ‘420A’, ‘1103P’, and V. rupestris were inoculated by immersion in a conidial suspension of the selected fungal Pch-sGFP71 line and incubated at 4 ± 1 and 25 ± 1°C. Wood colonization was estimated through epifluorescence microscopy and was affected by incubation temperature. After 6 months at 4 ± 1°C, the fungal growth was completely inhibited. At 25 ± 1°C, the highest extent of wood colonization was recorded in Montepulciano and Verdicchio, with the lowest in the rootstocks SO4 and V. rupestris. The expression of the Pch-sGFP71 transformed line was localized in the xylem area, primarily around the vessels. The use of sGFP-transformed P. chlamydospora helped to clarify different aspects associated with the location of this pathogen in grapevine tissue, before disease symptom expression.

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