scholarly journals Role of Blossom Colonization in Pepper Seed Infestation by Xanthomonas euvesicatoria

2014 ◽  
Vol 104 (3) ◽  
pp. 232-239 ◽  
Author(s):  
B. Dutta ◽  
R. Gitaitis ◽  
H. Sanders ◽  
C. Booth ◽  
S. Smith ◽  
...  
Keyword(s):  
Separate Experiment ◽  
Inoculum Concentration ◽  
Pepper Fruit ◽  
Polymerase Chain ◽  
Xanthomonas Euvesicatoria ◽  
The Relationship ◽  
Pepper Seed ◽  
Seed Infestation ◽  
Better Than

Colonization of Xanthomonas euvesicatoria was investigated in pepper blossoms and the relationship between inoculum concentrations and seed infestation was determined. Inoculation of blossoms resulted in asymptomatic pepper fruit. However, real-time polymerase chain reaction detected X. euvesicatoria in 39% of the seed lots assayed and viable colonies were recovered from 35% of them. Successful transmission occurred in 16% of the seed lots tested. In a separate experiment, X. euvesicatoria reached populations of up to 1 × 105 CFU/blossom on stigmas 96 h after inoculation. Bacteria colonized stylar and ovary tissues with populations ranging from 1 × 105 to 1 × 106 CFU/blossom 96 h after inoculation. A positive correlation existed between inoculum concentration and percentage of infested seedlots. Blossoms inoculated with Acidovorax citrulli also resulted in infested pepper seedlots. Furthermore, A. citrulli colonized pepper blossoms significantly better than X. euvesicatoria by 96 h postinoculation. It was concluded that pepper blossoms can be a potential site of ingress for X. euvesicatoria into seed, and blossom colonization may be involved in pepper seed infestation. Data also indicated that seed infestation via blossoms may be nonspecific because nonhost plants can be colonized by incompatible pathogens. Thus, host–pathogen interactions may not be important for bacterial ingress through blossoms.

Equity and the Right to Do Wrong

2020 ◽  
pp. 72-91
Author(s):  
Andrew S Gold
Keyword(s):  
The State ◽  
The Self ◽  
Role Of The State ◽  
The Right ◽  
The Relationship ◽  
Private Litigation ◽  
Better Than

This chapter discusses how the ‘stickler-enjoining’ account of equity has important limits. While many distinctive doctrines of equity can be understood to limit stickler behaviour, equity in fact often turns a blind eye to, and sometimes even enables, stickler behaviour. One can sort cases in which equity restrains sticklers from those in which it is indifferent to stickler behaviour if one attends to the role of the state in private litigation. Sometimes the state’s responsibilities require it to protect plaintiffs against sticklers. Other times, it requires it to protect the stickler, as a means, for example, of keeping as open as possible each person’s sphere of choices. Ultimately, the self-regarding account of equity sheds light on the question of the relationship between equity and justice: from the distinct perspective of the judgment, sometimes equitable justice is better than legal justice and sometimes legal justice is better than equitable justice.

scholarly journals Tipranavir (TPV) Genotypic Inhibitory Quotient Predicts Virological Response at 48 Weeks to TPV-Based Salvage Regimens

2007 ◽  
Vol 52 (3) ◽  
pp. 1066-1071 ◽  
Author(s):  
Daniel Gonzalez de Requena ◽  
Stefano Bonora ◽  
Andrea Calcagno ◽  
Antonio D'Avolio ◽  
Marco Siccardi ◽  
...  
Keyword(s):  
Virological Response ◽  
Protease Gene ◽  
Reverse Transcriptase Inhibitors ◽  
Cutoff Value ◽  
Multiple Drugs ◽  
The Relationship ◽  
Cell Count Increase ◽  
Better Than

ABSTRACT The virological response (VR) to a tipranavir-ritonavir (TPV-RTV)-based regimen had been shown to be associated with a number of mutations in the protease gene, the use of enfuvirtide (T20), and the TPV phenotypic inhibitory quotient (IQ). The role of the TPV genotypic IQ (gIQ) has not yet been fully investigated. The aim of our study was to evaluate the relationship between the TPV gIQ and the VR at 48 weeks to TPV-based salvage regimens. Patients placed on regimens containing two nucleoside reverse transcriptase inhibitors plus TPV-RTV 500/200 mg twice a day with or without T20 were prospectively studied. Regular follow-up was performed over the study period. VR, considered a viral load (VL) decrease of ≥1 log unit and/or the achievement of <50 copies/ml with no VL rebound of >0.5 log unit compared to the maximal VL decrease at week 48, was assessed. Thirty-eight patients who had received multiple drugs were included. At week 48 the VL decrease was −1.48 (interquartile range [IQR], −2.88 to −0.48), 15 patients (39.5%) had VLs of <50 copies/ml, and the CD4+ cell count increase was 37 cells/mm3 (IQR, −30 to +175). Twenty subjects (52.6%) achieved VRs. The TPV gIQ and optimized background score (OBS) were independently associated with higher VL decreases. The TPV gIQ and OBS were also independent predictors of a VR at week 48. TPV gIQ and OBS cutoff values of 14,500 and 2, respectively, were associated with a higher rate of VR. The TPV gIQ was shown to be able to predict the VR at 48 weeks to TPV-containing salvage regimens better than the TPV trough concentration or TPV-associated mutations alone. A possible TPV gIQ cutoff value of 14,500 for reaching a VR at week 48 was suggested. Further studies are needed in order to evaluate the calculation of TPV gIQ as a new tool for the optimization of TPV-based salvage therapy.

scholarly journals Methylation and Gene Expression of BCAT1 and IKZF1 in Colorectal Cancer Tissues

2018 ◽  
Vol 12 ◽  
pp. 117955491877506 ◽  
Author(s):  
Maher Jedi ◽  
Graeme P Young ◽  
Susanne K Pedersen ◽  
Erin L Symonds
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Messenger Rna ◽  
Stage Iv ◽  
Mrna Levels ◽  
Polymerase Chain ◽  
Cancer Tissues ◽  
The Relationship ◽  
Neoplastic Tissues

The genes BCAT1 and IKZF1 are hypermethylated in colorectal cancer (CRC), but little is known about how this relates to gene expression. This study assessed the relationship between methylation and gene expression of BCAT1 and IKZF1 in CRC and adjacent non-neoplastic tissues. The tissues were obtained at surgery from 36 patients diagnosed with different stages of CRC (stage I n = 8, stage II n = 13, stage III n = 10, stage IV n = 5). Methylated BCAT1 and IKZF1 were detected in 92% and 72% CRC tissues, respectively, with levels independent of stage ( P > .05). In contrast, only 31% and 3% of non-neoplastic tissues were methylated for BCAT1 and IKZF1, respectively ( P < .001). The IKZF1 messenger RNA (mRNA) expression was significantly lower in the cancer tissues compared with that of non-neoplastic tissues, whereas the BCAT1 mRNA levels were similar. The latter may be due to the BCAT1 polymerase chain reaction assay detecting more than 1 mRNA transcript. Further studies are warranted to establish the role of the epigenetic silencing of IKZF1 in colorectal oncogenesis.

scholarly journals Functional glyco-metagenomics elucidates the role of glycan-related genes in environments

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hayato Takihara ◽  
Nobuaki Miura ◽  
Kiyoko F. Aoki-Kinoshita ◽  
Shujiro Okuda
Keyword(s):  
Sequence Alignment ◽  
The Other ◽  
Glycoside Hydrolases ◽  
Optimal Parameters ◽  
Complete Genomes ◽  
Enzyme Families ◽  
The Relationship ◽  
Alignment Length ◽  
Better Than

Abstract Background Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear. Methods Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments. Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data. Results As a result, we identified 86.73 million glycan-related genes from the metagenome data. Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments. On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments. These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments. The general enzyme families were mostly from genes involved in monosaccharide metabolism, and most of the specific enzymes were polysaccharide degrading enzymes. Conclusion These findings suggest that environmental microorganisms could change the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments. Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.

scholarly journals Access to Medical Technologies: Do Gender and Social Capital matter?

2019 ◽  
Vol 23 (2) ◽  
pp. 23-31
Author(s):  
Mickaël Géraudel ◽  
Katherine Gundolf** ◽  
Beate Cesinger ◽  
Christina Constantinidis
Keyword(s):  
Social Capital ◽  
Negative Impact ◽  
Weak Ties ◽  
Network Ties ◽  
Private Practitioners ◽  
Medical Technologies ◽  
Social Network Ties ◽  
The Relationship ◽  
Better Than

We explore the relationship between the characteristics of social capital, the speed of access to medical technologies and the role of gender in a private practice context. Our findings from a sample of 98 German private practitioners show that: (a) being a woman has an overall negative impact on the speed of access to medical technologies; (b) private practitioners with strong social network ties obtain quicker access to medical technologies than do those with weak ties; (c) men relying on their weak ties perform better than women who do so. In contrast, we observe that women relying on strong ties outperform their male counterparts in terms of speed of access to medical technologies.

scholarly journals The Role of Employees’ Work Patterns and Office Type Fit (and Misfit) in the Relationships Between Employee Well-Being and Performance

2018 ◽  
Vol 52 (2) ◽  
pp. 111-138 ◽  
Author(s):  
Aida Soriano ◽  
Malgorzata W. Kozusznik ◽  
José M. Peiró ◽  
Carolina Mateo
Keyword(s):  
Positive Emotions ◽  
Well Being ◽  
Role Performance ◽  
Work Patterns ◽  
Work Pattern ◽  
Work Tasks ◽  
And Performance ◽  
The Relationship ◽  
Better Than

Nearly half of workers agree that their workspace is unsuitable for their work tasks. Furthermore, it is assumed that happy workers often perform better than unhappy ones. Nevertheless, due to the effect of the emotional-cognitive processes, the misfit between employees’ office type and their work patterns (complexity and interactivity) may hamper this relationship between well-being and performance. This diary study on 83 office workers ( n = 603 time points) combines information about work patterns identified by using cluster analysis with Neufert’s office type classification. Results show that the work pattern–office type (mis)fit moderates the relationship between well-being and performance. The “fit” group shows four out of six positive associations: flow and positive emotions with in-role performance, and positive emotions and activity worthwhileness with extra-role performance. The “misfit” group shows only one out of six positive associations. Thus, the office environment–work pattern fit has a relationship to in-role and extra-role performance.

Analysis of the association of polymorphisms CASR and VDR with the development of nephrolithiasis in patients with primary hyperparathyroidism

2016 ◽  
Vol 61 (5) ◽  
pp. 4-8 ◽  
Author(s):  
Elena Viktorovna Peretokina ◽  
Ekaterina Aleksandrovna Pigarova ◽  
Natal'ya Georgievna Mokrysheva ◽  
Lyudmila Yakovlevna Rozhinskaya ◽  
Galina Viktorovna Baydakova ◽  
...  
Keyword(s):  
Vitamin D ◽  
Gene Polymorphisms ◽  
Restriction Analysis ◽  
Phosphorus Metabolism ◽  
Chain Reaction ◽  
Calcium Phosphorus ◽  
Polymerase Chain ◽  
Relationship Of ◽  
The Relationship

Pathogenesis of nephrolithiasis (NL) at PHPT is not fully understood. Meanwhile, the detection of NL patients with PHPT is an absolute indication for parathyroidectomy. Conducted various studies aimed at finding a predictor of NL patients with PHPT. Actively study the role of genetic markers, particularly genes that regulate calcium-phosphorus metabolism.Objective — to assess the relationship of polymorphisms CASR and VDR with the development of the NL at PHPТ.Material and methods. A study to include 203 patients with confirmed PHPT, out of which 114 patients had the NL and 87 patients without NL. All patients were studied indicators calcium-phosphorus metabolism, the study of the level of PTH, vitamin D, the filtration function of the kidneys. All patients were studied indicators calcium-phosphorus metabolism, the level of PTH, vitamin D, the filtration function of the kidneys. The study of gene polymorphisms VDR (FokI, TaqI, BsmI, ApaI, Cdx2) was performed in 169 patients (113 with NL, 56 without NL) by polymerase chain reaction followed by restriction analysis; Study 3 polymorphisms CASR (A986S, R990G, Q1011E) was performed in 187 patients (110 with NL, 77 without NL), by direct sequencing.Results. No significant differences in the frequency of genotypes and alleles studied genes between the two groups has been received. According to logistic regression analysis, the only predictor of NL is the level of ionized calcium.Conclusions. The studied genes can not be used as predictors of the NL. May need to investigate other genes.

scholarly journals The Escherichia coli O157 Flagellar Regulatory Gene flhC and Not the Flagellin Gene fliC Impacts Colonization of Cattle

2006 ◽  
Vol 74 (5) ◽  
pp. 2894-2905 ◽  
Author(s):  
Heather S. Dobbin ◽  
Carolyn J. Hovde ◽  
Christopher J. Williams ◽  
Scott A. Minnich
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Oral Dose ◽  
Regulatory Gene ◽  
Wild Type ◽  
E Coli ◽  
Regulatory Mutant ◽  
The Relationship ◽  
Better Than

ABSTRACT A virulent European Escherichia coli O157:H − isolate is nonmotile due to a 12-bp deletion in the flagellar regulatory gene flhC. To investigate the contribution of flhC in the relationship between E. coli O157:H7 and cattle, we constructed a similar flhC regulatory mutant in the well-characterized strain ATCC 43894. There was no difference in the growth rate between the wild type and this regulatory mutant, but phenotypic arrays showed substrate utilization differences. Survival in the bovine gastrointestinal tract and colonization of the rectoanal junction mucosa were assessed. Mixtures of both strains were given orally or rectally to steers or administered into the rumen of cattle dually cannulated at the rumen and duodenum. One day post-oral dose, most rectal/fecal isolates (74%) were the regulatory mutant, but by 3 days post-oral dose and throughout the 42-day experiment, ≥80% of the isolates were wild type. Among steers given a rectal application of both strains, wild-type isolates were the majority of isolates recovered on all days. The regulatory mutant survived better than the wild type in both the rumen and duodenum. To test the role of motility, a filament mutant (ΔfliC) was constructed and similar cattle experiments were performed. On all days post-oral dose, the majority of isolates (64% to 98%) were the filament mutant. In contrast, both strains were recovered equally post-rectal application. Thus, the regulatory mutant survived passage through the bovine gastrointestinal tract better than the wild type but failed to efficiently colonize cattle, and the requirement of flhC for colonization was not dependent on a functional flagellum.

scholarly journals The Role of Apoptosis and Autophagy in Bovine Abortions Associated with Brucella Spp

2016 ◽  
Vol 66 (1) ◽  
pp. 37-50 ◽  
Author(s):  
Mustafa Ozkaraca ◽  
Songul Ceribasi ◽  
Ali Osman Ceribasi ◽  
Ayse Kilic ◽  
Hasan Ongor
Keyword(s):  
Skeletal Muscle ◽  
Lymph Nodes ◽  
Caspase 3 ◽  
Natural Infection ◽  
Cellular Death ◽  
Thymus Tissue ◽  
Bovine Fetuses ◽  
Polymerase Chain ◽  
The Relationship

AbstractThis study is aimed to evaluate the relationship between the severity of apoptotic and autophagic cell death based on the distribution of Brucella spp. antigens in the lung, liver, kidney, spleen, brain, heart, skeletal muscle, mesenteric lymph node, and thymus tissue from bovine fetuses aborted due to natural infection with Brucella spp. The distribution of Brucella spp. antigens was immunohistochemically examined in the tissues of 16 aborted fetuses from cattle diagnosed with Brucella spp. infection by a polymerase chain reaction (PCR). In addition, immunostaining of primary antibodies for cleaved caspase 3 was performed to detect apoptosis, and immunostaining of Microtubule Associated Protein 1 Light Chain 3 Beta (LC3B) was used to detect autophagy in the Brucella spp.- related abortions. Analysis of cellular death revealed strong immunopositivity in the lung, spleen, kidney, and thymus, moderate immunopositivity in the liver, mesenterial lymph nodes, and heart muscle and slight immunopositivity in the brain and skeletal muscle by staining of Brucellaspp. antigens. According to the immunohistochemical results, the immunopositivity of cleaved caspase 3 and LC3B was extremely high in the lung, thymus, spleen, kidney, and liver tissues. The immunostaining of cleaved caspase 3 in the lung, thymus, and kidney tissues was severe compared to that of LC3B. In the liver, spleen, and mesenterial lymph nodes, the immunopositivity of LC3B was higher than that of cleaved caspase 3. Bacterial antigens were highly evident in the lung, spleen, kidney, and thymus tissues of Brucella spp.-related bovine abortions, and both apoptosis and autophagy played a role in cellular death.

scholarly journals Role of Blossoms in Watermelon Seed Infestation by Acidovorax avenae subsp. citrulli

2003 ◽  
Vol 93 (5) ◽  
pp. 528-534 ◽  
Author(s):  
R. R. Walcott ◽  
R. D. Gitaitis ◽  
A. C. Castro
Keyword(s):  
Polymerase Chain Reaction ◽  
Relative Humidity ◽  
Immunomagnetic Separation ◽  
Chain Reaction ◽  
Pantoea Ananatis ◽  
Bacterial Fruit Blotch ◽  
Watermelon Seed ◽  
Polymerase Chain ◽  
Seed Infestation

The role of watermelon blossom inoculation in seed infestation by Acidovorax avenae subsp. citrulli was investigated. Approximately 98% (84/87) of fruit developed from blossoms inoculated with 1 × 107 or 1 × 109 CFU of A. avenae subsp. citrulli per blossom were asymptomatic. Using immunomagnetic separation and the polymerase chain reaction, A. avenae subsp. citrulli was detected in 44% of the seed lots assayed, despite the lack of fruit symptoms. Furthermore, viable colonies were recovered from 31% of the seed lots. Of these lots, 27% also yielded seedlings expressing bacterial fruit blotch symptoms when planted under conditions of 30°C and 90% relative humidity. A. avenae subsp. citrulli was detected and recovered from the pulp of 33 and 19%, respectively, of symptomless fruit whose blossoms were inoculated with A. avenae subsp. citrulli. The ability to penetrate watermelon flowers was not unique to A. avenae subsp. citrulli, because blossoms inoculated with Pantoea ananatis also resulted in infested seed and pulp. The data indicate that watermelon blossoms are a potential site of ingress for fruit and seed infestation by A. avenae subsp. citrulli.

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