Early protective joint motion influences gene expression in a rat model of anterior cruciate complete rupture

The urgency of the search for inexpensive and effective drugs with localized action for the treatment of rheumatoid arthritis continues unabated. In this study, for the first time we investigated the Cytos-11 antisense oligonucleotide suppression of TNF-α gene expression in a rat model of rheumatoid arthritis induced by complete Freund’s adjuvant. Cytos-11 has been shown to effectively reduce peripheral blood concentrations of TNF-α, reduce joint inflammation, and reduce pannus development. The results achieved following treatment with the antisense oligonucleotide Cytos-11 were similar to those of adalimumab (Humira®); they also compared favorably with those results, which provides evidence of the promise of drugs based on antisense technologies in the treatment of this disease.

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Influence of the site of injury on the spontaneous healing response in a rat model of total rupture of the anterior cruciate ligament

Connective Tissue Research ◽

10.1080/03008207.2021.1889529 ◽

2021 ◽

Author(s):

Takuma Kano ◽

Takanori Kokubun ◽

Kenji Murata ◽

Yuichiro Oka ◽

Kaichi Ozone ◽

...

Keyword(s):

Anterior Cruciate Ligament ◽

Rat Model ◽

Cruciate Ligament ◽

Spontaneous Healing ◽

Healing Response ◽

Anterior Cruciate

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Cruciate Ligament Cell Sheets Can Be Rapidly Produced on Thermoresponsive poly(glycidyl ether) Coating and Successfully Used for Colonization of Embroidered Scaffolds

Cells ◽

10.3390/cells10040877 ◽

2021 ◽

Vol 10 (4) ◽

pp. 877

Author(s):

Ingrid Zahn ◽

Daniel David Stöbener ◽

Marie Weinhart ◽

Clemens Gögele ◽

Annette Breier ◽

...

Keyword(s):

Gene Expression ◽

Type I Collagen ◽

Cruciate Ligament ◽

Glycidyl Ether ◽

Stable Gene ◽

Type I ◽

Cell Sheets ◽

Thermoresponsive Polymers ◽

Related Phenotype ◽

Anterior Cruciate

Anterior cruciate ligament (ACL) cell sheets combined with biomechanically competent scaffolds might facilitate ACL tissue engineering. Since thermoresponsive polymers allow a rapid enzyme-free detachment of cell sheets, we evaluated the applicability of a thermoresponsive poly(glycidyl ether) (PGE) coating for cruciate ligamentocyte sheet formation and its influence on ligamentocyte phenotype during sheet-mediated colonization of embroidered scaffolds. Ligamentocytes were seeded on surfaces either coated with PGE or without coating. Detached ligamentocyte sheets were cultured separately or wrapped around an embroidered scaffold made of polylactide acid (PLA) and poly(lactic-co-ε-caprolactone) (P(LA-CL)) threads functionalized by gas-phase fluorination and with collagen foam. Ligamentocyte viability, protein and gene expression were determined in sheets detached from surfaces with or without PGE coating, scaffolds seeded with sheets from PGE-coated plates and the respective monolayers. Stable and vital ligamentocyte sheets could be produced within 24 h with both surfaces, but more rapidly with PGE coating. PGE did not affect ligamentocyte phenotype. Scaffolds could be colonized with sheets associated with high cell survival, stable gene expression of ligament-related type I collagen, decorin, tenascin C and Mohawk after 14 d and extracellular matrix (ECM) deposition. PGE coating facilitates ligamentocyte sheet formation, and sheets colonizing the scaffolds displayed a ligament-related phenotype.

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Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/3575231 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Zhenyu Wu ◽

Xiaofang Lu ◽

Shengsheng Zhang ◽

Chunyang Zhu

Keyword(s):

Gene Expression ◽

Dorsal Horn ◽

Functional Dyspepsia ◽

Rat Model ◽

Spinal Dorsal Horn ◽

Visceral Hypersensitivity ◽

Enzyme Linked Immunosorbent Assay ◽

Gastric Distention ◽

Real Time Quantitative Pcr ◽

Spinal Dorsal

The present study investigated the effect of Chinese medicine Sini-San (SNS) on visceral hypersensitivity in a rat model of functional dyspepsia (FD), and it explored related underlying mechanisms. The rat model of FD was developed by combining neonatal iodoacetamide (IA) treatment and adult tail-clamping. After SNS treatment, the behavior and electromyographic testing were performed to evaluate the visceromotor responses of rats to gastric distention. Immunofluorescence was used to detect the distribution of iNOS-positive cells in the spinal dorsal horn, while the real-time quantitative PCR and western blot were used for detection of the gene expression of c-fos, iNOS, and GABAb and protein levels of iNOS and GABAb in the spinal dorsal horn, respectively. The protein concentration of cGMP and PKG proteins in the spinal dorsal horn were quantified by enzyme-linked immunosorbent assay. In this study, SNS treatment significantly reduced the behavioral score and electromyographic response to graded intragastric distension pressure. The middle-dose of SNS treatment significantly reduced the distribution of iNOS-positive cells in the spinal dorsal horn of FD model rats. The gene expression of c-fos, iNOS, and GABAb and the protein contents of iNOS, GABAb, cGMP, and PKG in the spinal dorsal horn of FD model rats were restored to a normal level by middle-dose of SNS treatment. Our results suggest that Sini-San may alleviate the visceral hypersensitivity in FD model rats via regulation of the NO/cGMP/PKG pathway in the spinal dorsal horn.

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