Omega 3 Fatty Acids Counteract IL-8 and Prostaglandin E2 Secretion                     Induced by TNF-α in Cultured Endometrial Stromal Cells

Background Inflammation is part of the normal host response to infection and injury and excess inflammation may lead to acute or chronic diseases. Endometriosis is a common gynecologic disease characterized by increased endometrial production of inflammatory mediators such as cytokines, reactive oxygen species, and prostaglandins. Omega-3 polyunsaturated fatty acids play a role in the inflammatory process and a therapeutic effect has been suggested. Methods On this basis, this study aimed to assess in human cultured endometrial stromal cells the effect of two omega-3 fatty acids (DHA and EPA), on i) TNF-α induced IL-8 and PGE2 release and on ii) cyclooxygenase-2 (Cox-2) mRNA expression a key enzyme for prostaglandin production. Results DHA or EPA significantly reduced LPS-induced IL-8 (P<0.01) and PGE2 (P<0.01) release decreasing the expression of Cox-2 (P<0.01). Conclusions In conclusion, our study revealed an inhibitory effect of omega-3 fatty acids on inflammatory mediators secretion and suggests a potential benefit for the treatment of pelvic pain in patients with dysmenorrhea and/or endometriosis.

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Inhibitory effect of omega-3 fatty acids on alveolar bone resorption and osteoclast differentiation

Journal of Oral Science ◽

10.2334/josnusd.19-0267 ◽

2020 ◽

Vol 62 (3) ◽

pp. 298-302 ◽

Cited By ~ 1

Author(s):

Yu Ozaki ◽

Toshiya Morozumi ◽

Kiyoko Watanabe ◽

Toshizo Toyama ◽

Haruka Sasaki ◽

...

Keyword(s):

Fatty Acids ◽

Bone Resorption ◽

Alveolar Bone ◽

Osteoclast Differentiation ◽

Inhibitory Effect ◽

Omega 3 Fatty Acids ◽

Omega 3

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INFLUENCE OF A PERIOPERATIVE NUTRITION ENRICHED WITH OMEGA-3 FATTY ACIDS ON THE SYNTHESIS OF INFLAMMATORY MEDIATORS FROM PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH MAJOR SURGERY.

Shock ◽

10.1097/00024382-199703001-00607 ◽

1997 ◽

Vol 7 (Supplement) ◽

pp. 150

Author(s):

M. Köller ◽

P. Wachtler ◽

M. Senkal ◽

M. Kemen ◽

W. König

Keyword(s):

Fatty Acids ◽

Inflammatory Mediators ◽

Peripheral Blood ◽

Major Surgery ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Perioperative Nutrition

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Fermentation Improves Anti-Inflammatory Effect of Sipjeondaebotang on LPS-Stimulated RAW 264.7 Cells

The American Journal of Chinese Medicine ◽

10.1142/s0192415x12500619 ◽

2012 ◽

Vol 40 (04) ◽

pp. 813-831 ◽

Cited By ~ 10

Author(s):

You-Chang Oh ◽

Won-Kyung Cho ◽

Yun Hee Jeong ◽

Ga Young Im ◽

Min Cheol Yang ◽

...

Keyword(s):

Nitric Oxide ◽

Inflammatory Mediators ◽

High Performance ◽

Biological Effects ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Tnf Α ◽

Cox 2 ◽

Anti Inflammatory

Sipjeondaebotang (SJ) has been used as a traditional drug in east-Asian countries. In this study, to provide insight into the biological effects of SJ and SJ fermented by Lactobacillus, we investigated their effects on lipopolysaccharide (LPS)-mediated inflammation in macrophages. The investigation was focused on whether SJ and fermented SJ could inhibit the production of pro-inflammatory mediators such as prostaglandin (PG) E2 and nitric oxide (NO) as well as the expressions of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB in LPS-stimulated RAW 264.7 cells. We found that SJ modestly inhibited LPS-induced PGE2, NO and TNF-α production as well as the expressions of COX-2 and iNOS. Interestingly, fermentation significantly increased its inhibitory effect on the expression of all pro-inflammatory mediators. Furthermore, fermented SJ exhibited increased inhibition of p38 MAPK and c-Jun NH2-terminal kinase (JNK) MAPK phosphorylation as well as NF-κB p65 translocation by reduced IκBα degradation compared with either untreated controls or unfermented SJ. High performance liquid chromatography (HPLC) analysis showed fermentation by Lactobacillus increases liquiritigenin and cinnamyl alcohol contained in SJ, which are known for their anti-inflammatory activities. Finally, SJ fermented by Lactobacillus exerted potent anti-inflammatory activity by inhibiting MAPK and NF-κB signaling in RAW 264.7 cells.

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Effect of docosahexaenoic acid plus insulin on atherosclerotic human endothelial cells

10.21203/rs.3.rs-50793/v2 ◽

2020 ◽

Author(s):

Aysan Eslami Abriz ◽

Reza Rahbarghazi ◽

Alireza Nourazarian ◽

Çıgır Biray Avci ◽

Soltan Ali Mahboob ◽

...

Keyword(s):

Fatty Acids ◽

Endothelial Cells ◽

Docosahexaenoic Acid ◽

Palmitic Acid ◽

Pcr Array ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Human Endothelial Cells ◽

Array Analysis ◽

Tnf Α

Abstract Background: Atherosclerosis is touted as one of the most critical consequences of diabetes mellitus indicated by local inflammation of endothelial cells. The Effect of Omega 3 fatty acids, mainly docosahexaenoic acid (DHA), has been investigated in cells after exposure to high doses of lipids. The current experiment aimed to address the modulatory effects of docosahexaenoic acid and insulin in palmitic-treated human endothelial cells. Methods: Human umbilical vein endothelial cells were treated with 1mM palmitic acid, 50μM insulin, 50μM docosahexaenoic acid, and their combination for 48 hours. Cell survival rate and apoptosis were measured using MTT and flow cytometry assays. The Griess assay detected NO levels. Protein levels of TNF-α, IL-6, and NF-κB were studied using ELISA and immunofluorescence imaging. The expression of genes participating in atherosclerosis was monitored using PCR array analysis. Results: Oil Red O staining showed the inhibitory effect of DHA and insulin to reduce the intracellular accumulation of palmitic acid. Both DHA and Insulin blunted palmitic acid detrimental effects on HUVECs indicated by an increased survival rate (p<0.05). The percent of apoptotic cells was decreased in palmitic-treated cells received insulin and DHA compared to palmitic-treated group (p<0.05). Based on our data, DHA and Insulin diminished the production of all inflammatory cytokines, TNF-α, IL-6, and NF-κB, in palmitic-treated cells (p<0.05). Similar to these data, NO production was also decreased in all groups treated with insulin and DHA compared to the palmitic-treated cells (p<0.05). PCR array analysis revealed the modulatory effect of DHA and insulin on the expression of atherosclerosis-related genes pre-treated with palmitic acid compared to the control group (p<0.05). Conclusion: DHA and Insulin could alter the dynamic growth and dysfunctional activity of human endothelial cells after treatment with palmitic acid. Taken together, Omega 3 fatty acids, along with insulin, could dictate specific cell behavior in endothelial cells in vitro

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