The impact of amalgam dental fillings on the frequency of Helicobacter pylori infection and H. pylori eradication rates in patients treated with concomitant, quadruple, and levofloxacin-based therapies

Helicobacter pylori infection concerns half of the world’s population, mainly in developing countries. It causes several gastrodudenal pathologies such as gastritis, ulcer and gastric adenocarcinoma. The aim of our study was to determine the prevalence of H.pylori infection and to assess the impact of different epidemiological factors as well as principal gastric diseases associ-ated to this infection. We underwent a prospective study during 18 months (month 2016-month 2017) which implicated 201 symptomatic patients for gastric fiboptic endoscopy at the level of Sidi Bel Abbes University hospital. We collected patients’ biopsies to perform a histological study and H. pylori culture. H. pylori identification was carried out based on bacteriological and biochemical analysis. The middle age of our population was (47.29 ±15.97ans) and the sex-ratio =0,8. The global prevalence of Helicobacter pylori infection is of 61.2% (123/201). This rate, after a statistic analysis, seems to be significantly related to age. It is particularly high especially for patients belonging to age range (20-30)-(51-60) years. The gender did not affect the infection prevalence that is more frequent in the gastritis case. We noticed also that HP infection prevalence was important in SBA the hospital. The range age (20-30)-(51-60) years had the highest prevalence of H. pylori and of gastritis which might be a risky ground of gastric cancer appearance. The ulcer pathology maximal rate concerned the group of 51 to 60 years. Above this age, this rate dropped whereas the number of patients suffering from gastric cancer, which presents an important rate in our study, increase for the group of 61-70 years.

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The Changing Prevalence of Helicobacter Pylori Infection in Canadian Children: Should Screening Be Performed in High-Risk Children?

Canadian Journal of Gastroenterology ◽

10.1155/2005/840909 ◽

2005 ◽

Vol 19 (7) ◽

pp. 412-414 ◽

Cited By ~ 15

Author(s):

Kevan Jacobson

Keyword(s):

Socioeconomic Status ◽

Helicobacter Pylori ◽

Eradication Therapy ◽

Developed Countries ◽

Living Conditions ◽

Helicobacter Pylori Infection ◽

Populations At Risk ◽

H Pylori ◽

The Impact ◽

First World

While several studies have demonstrated a decline in the overall prevalence of Helicobacter pylori infection in developed countries, there is variability in the burden of infection linked to socioeconomic status and living conditions. Improved socioeconomic status, living conditions and the availability of H pylori-eradication therapy have been associated with a lower prevalence of infection in First World populations, yet immigrants and indigenous people continue to have a high burden of H pylori infection and disease. Although the changing prevalence of H pylori infection in children has been recognized in a few reports, further studies are required to determine the impact of H pylori infection in this population. Moreover, additional studies are required for those populations at risk.

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Diagnosis of Helicobacter pylori Infection in a Routine Testing Workflow: Effect of Bacterial Load and Virulence Factors

Journal of Clinical Medicine ◽

10.3390/jcm10132755 ◽

2021 ◽

Vol 10 (13) ◽

pp. 2755

Author(s):

Nabil Gastli ◽

Margaux Allain ◽

Dominique Lamarque ◽

Vered Abitbol ◽

Annick Billoët ◽

...

Keyword(s):

Helicobacter Pylori ◽

Bacterial Load ◽

Diagnostic Methods ◽

Helicobacter Pylori Infection ◽

23S Rrna ◽

Lower Sensitivity ◽

Caga Status ◽

High Bacterial Load ◽

H Pylori ◽

The Impact

Reliable diagnostic methods are mandatory for effective management of Helicobacter pylori infection. Histology and culture are the most common invasive methods in current practice, even if molecular methods are gaining in importance. The performance of these conventional methods varies significantly. We conducted a retrospective study of 1540 adults and 504 children with gastric biopsies taken during endoscopy to assess the impact of bacterial load and the cagA virulence factor on the performance of H. pylori infection testing. The association between virulence and histology findings was also investigated. With 23S rRNA qPCR confirmed by glmM amplification as the gold standard, culture and histology had lower sensitivity, 74.4% and 73.3%, respectively. However, their sensitivity was enhanced (>90%) in biopsies with high bacterial load (qPCR Ct < 30). Positive cagA status of the strain was associated with high bacterial load (94.9%), thus resulting in more frequent positive culture (94.3%) and H. pylori histology detection (91.7%) and more severe lesions on histology (p < 0.001). Conversely, the cagA status of the strains was negative in 110/119 (92.4%) of biopsies with low bacterial load (qPCR Ct < 30), 82/90 (91.1%) with negative H. pylori histology detection and 119/131 (90%) with negative culture findings (p < 0.001). This study highlights the low sensitivity of conventional culture and histology that may lead to false negative diagnosis if used alone. H. pylori quantification associated with cagA genotyping in routine workflow are essential for a sensitive and reliable diagnosis, to identify patients at high risk and to manage eradication therapies.

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Faculty Opinions recommendation of Toxigenic Helicobacter pylori infection precedes gastric hypochlorhydria in cancer relatives, and H. pylori virulence evolves in these families.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1103709.560926 ◽

2008 ◽

Author(s):

Shafiqul A Sarker

Keyword(s):

Helicobacter Pylori ◽

Helicobacter Pylori Infection ◽

H Pylori

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A Retrospective Experience of Helicobacter pylori Histology in a Large Sample of Subjects in Northern Italy

Life ◽

10.3390/life11070650 ◽

2021 ◽

Vol 11 (7) ◽

pp. 650

Author(s):

Davide Giuseppe Ribaldone ◽

Carlo Zurlo ◽

Sharmila Fagoonee ◽

Chiara Rosso ◽

Angelo Armandi ◽

...

Keyword(s):

Helicobacter Pylori ◽

Large Population ◽

Population Data ◽

Signet Ring Cell ◽

Current Data ◽

Low Grade ◽

Gastric Cardia Adenocarcinoma ◽

Significant Difference ◽

H Pylori ◽

The Impact

Updated data about the prevalence of Helicobacter pylori (H. pylori) and its correlation with histological results are scarce. The aim of our study was to provide current data on the impact of H. pylori in a third-level endoscopy service. We performed a large, retrospective study analyzing the results of all histological samples of gastroscopy from the year 2019. In total, 1512 subjects were included. The prevalence of H. pylori was 16.8%. A significant difference between the prevalence in subjects born in Italy and those from eastern Europe, south America, or Africa was found (p < 0.0001, p = 0.006, and p = 0.0006, respectively). An association was found between H. pylori and active superficial gastritis (p < 0.0001). Current H. pylori and/or a previous finding of H. pylori was related to antral atrophy (p < 0.0001). Fifteen patients had low-grade dysplasia. There were no statistically significant associations with current or past H. pylori infection. One patient presented gastric cardia adenocarcinoma with regular gastric mucosa. One patient, H. pylori positive, was diagnosed with gastric signet ring cell adenocarcinoma in a setting of diffuse atrophy, without metaplasia.. Our study provides updated, solid (biopsy diagnosis and large population) data on the prevalence of H. pylori infection in a representative region of southern Europe.

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A High-Throughput Metabolic Microarray Assay Reveals Antibacterial Effects of Black and Red Raspberries and Blackberries against Helicobacter pylori Infection

Antibiotics ◽

10.3390/antibiotics10070845 ◽

2021 ◽

Vol 10 (7) ◽

pp. 845

Author(s):

Candace Goodman ◽

Katrina N. Lyon ◽

Aitana Scotto ◽

Cyra Smith ◽

Thomas A. Sebrell ◽

...

Keyword(s):

Helicobacter Pylori ◽

High Throughput ◽

Antibacterial Properties ◽

Treatment Strategies ◽

Helicobacter Pylori Infection ◽

Antibacterial Effects ◽

Biolog System ◽

Freeze Dried ◽

H Pylori

Helicobacter pylori infection is commonly treated with a combination of antibiotics and proton pump inhibitors. However, since H. pylori is becoming increasingly resistant to standard antibiotic regimens, novel treatment strategies are needed. Previous studies have demonstrated that black and red berries may have antibacterial properties. Therefore, we analyzed the antibacterial effects of black and red raspberries and blackberries on H. pylori. Freeze-dried powders and organic extracts from black and red raspberries and blackberries were prepared, and high-performance liquid chromatography was used to measure the concentrations of anthocyanins, which are considered the major active ingredients. To monitor antibiotic effects of the berry preparations on H. pylori, a high-throughput metabolic growth assay based on the Biolog system was developed and validated with the antibiotic metronidazole. Biocompatibility was analyzed using human gastric organoids. All berry preparations tested had significant bactericidal effects in vitro, with MIC90 values ranging from 0.49 to 4.17%. Antimicrobial activity was higher for extracts than powders and appeared to be independent of the anthocyanin concentration. Importantly, human gastric epithelial cell viability was not negatively impacted by black raspberry extract applied at the concentration required for complete bacterial growth inhibition. Our data suggest that black and red raspberry and blackberry extracts may have potential applications in the treatment and prevention of H. pylori infection but differ widely in their MICs. Moreover, we demonstrate that the Biolog metabolic assay is suitable for high-throughput antimicrobial susceptibility screening of H. pylori.

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Epidemiology of upper gastrointestinal bleeding and Helicobacter pylori infection: review of 3,488 Thai patients

Asian Biomedicine ◽

10.5372/1905-7415.0901.373 ◽

2017 ◽

Vol 9 (1) ◽

pp. 87-93

Author(s):

Sakolwan Suchartlikitwong ◽

Kamolyut Lapumnuaypol ◽

Rungsun Rerknimitr ◽

Duangporn Werawatganon

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Gastrointestinal Bleeding ◽

Upper Gastrointestinal Bleeding ◽

Helicobacter Pylori Infection ◽

Exclusion Criteria ◽

Common Causes ◽

Mucosal Lesions ◽

H Pylori ◽

Upper Gastrointestinal

Abstract Background The current epidemiology of upper gastrointestinal bleeding (UGIB) in Thailand is poorly understood and the reported prevalence of Helicobacter pylori infection is outdated. Objectives To investigate the etiologies of UGIB and prevalence of H. pylori infection in Thailand, including its association with UGIB. Methods We retrieved information regarding patients attending the endoscopic unit of King Chulalongkorn Memorial Hospital from June 2007 to January 2013. A database search using keywords “upper gastrointestinal bleeding” and “iron deficiency” was used. From 4,454 diagnoses, after exclusion criteria, 3,488 patients (2,042 male (58.5%) and 1,446 female (41.5%); mean age 63.3 ± 15.94 years, range 13–103 years) were included. Results The three most common causes of UGIB were peptic ulcer (38.2%), nonulcer-mucosal lesions (23.4%), and esophageal-related causes (20.4%). The 5 year-incidence of H. pylori was 25%–30%. The overall prevalence was 27%. The prevalence of H. pylori infection was found to decrease with age from 43.8% at <40 years to 21.7% at >79 years old. H. pylori infection was significantly associated with duodenal and gastroduodenal ulcers. Cirrhosis and nonulcer-mucosal lesions were significantly unrelated to H. pylori infection. Patients with concurrent cirrhosis with peptic ulcer were found to be negative for H. pylori infection. Conclusion Peptic ulcer is the leading cause of UGIB in Thailand. However, its incidence is declining. Patients who presented to hospital with UGIB were older, compared with those a decade ago. H. pylori infection plays an important role in UGIB and its incidence was stable during the past 5 years.

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Canadian Helicobacter Study Group Consensus Conference: Update on the Approach to Helicobacter Pylori Infection in Children and Adolescents – an Evidence-Based Evaluation

Canadian Journal of Gastroenterology ◽

10.1155/2005/390932 ◽

2005 ◽

Vol 19 (7) ◽

pp. 399-408 ◽

Cited By ~ 77

Author(s):

Nicola L Jones ◽

Philip Sherman ◽

Carlo A Fallone ◽

Nigel Flook ◽

Fiona Smaill ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Proton Pump Inhibitor ◽

Proton Pump ◽

Consensus Conference ◽

Helicobacter Pylori Infection ◽

Diagnostic Tools ◽

Study Group ◽

Evidence Based ◽

H Pylori

As an update to previously published recommendations for the management of Helicobacter pylori infection, an evidence-based appraisal of 14 topics was undertaken in a consensus conference sponsored by the Canadian Helicobacter Study Group. The goal was to update guidelines based on the best available evidence using an established and uniform methodology to address and formulate recommendations for each topic. The degree of consensus for each recommendation is also presented. The clinical issues addressed and recommendations made were: population-based screening for H pylori in asymptomatic children to prevent gastric cancer is not warranted; testing for H pylori in children should be considered if there is a family history of gastric cancer; the goal of diagnostic interventions should be to determine the cause of presenting gastrointestinal symptoms and not the presence of H pylori infection; recurrent abdominal pain of childhood is not an indication to test for H pylori infection; H pylori testing is not required in patients with newly diagnosed gastroesophageal reflux disease; H pylori testing may be considered before the use of long-term proton pump inhibitor therapy; testing for H pylori infection should be considered in children with refractory iron deficiency anemia when no other cause has been found; when investigation of pediatric patients with persistent or severe upper abdominal symptoms is indicated, upper endoscopy with biopsy is the investigation of choice; the 13C-urea breath test is currently the best noninvasive diagnostic test for H pylori infection in children; there is currently insufficient evidence to recommend stool antigen tests as acceptable diagnostic tools for H pylori infection; serological antibody tests are not recommended as diagnostic tools for H pylori infection in children; first-line therapy for H pylori infection in children is a twice-daily, triple-drug regimen comprised of a proton pump inhibitor plus two antibiotics (clarithromycin plus amoxicillin or metronidazole); the optimal treatment period for H pylori infection in children is 14 days; and H pylori culture and antibiotic sensitivity testing should be made available to monitor population antibiotic resistance and manage treatment failures.

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Study on Association between Chronic Idiopathic Urticaria and Helicobacter Pylori Infection in Armed Forces Personnel

Journal of Armed Forces Medical College Bangladesh ◽

10.3329/jafmc.v12i2.41108 ◽

2016 ◽

Vol 12 (2) ◽

pp. 122-126 ◽

Cited By ~ 1

Author(s):

Md Sayeed Hasan ◽

Md Shirajul Islam Khan ◽

Jannatun Nayeem

Keyword(s):

Helicobacter Pylori ◽

Eradication Therapy ◽

Armed Forces ◽

Case Control ◽

Chronic Idiopathic Urticaria ◽

Helicobacter Pylori Infection ◽

Enteric Infection ◽

Clinical Disorder ◽

Serum Igg ◽

H Pylori

Introduction: Chronic Idiopathic Urticaria (CIU) has an estimated prevalence of 35-65% and impacts 15 to 25% of the population at some point in their lives. Studies have shown the possible involvement of Helicobacter pylori (H. pylori) infection in chronic idiopathic urticaria (CIU), but the relationship remains controversial. Objective: To quantitatively assess the association between H. pylori infection and chronic idiopathic urticaria. Materials and Methods: This was a case-control type of analytical study and 100 patients were enrolled fifty patients of CIU fulfilling inclusion criteria with no identifiable cause were taken as case and patients without urticaria were taken as control, attending in the department of Dermatology & Venereology, CMH Dhaka for treatment from May 2015 to Aug 2016. Helicobacter pylori infection was confirmed by serum IgG for H. pylori test. Results: The result showed that H. pylori infection significantly affected a high percentage of patients 30 (60%) with chronic idiopathic urticaria. Male respondents were more positive 16(53.3%) than female 14(46.7%), in the middle aged 31-50 year about 18(60%) and triple drug therapy was successful in 16(53.33%) patients in whom H. pylori was detected. Conclusion: Urticaria is a common clinical disorder with complex triggering factors. Chronic urticaria has provided evidence that enteric infection with H. pylori may induce the disease. In this case control study, it was evident that chronic idiopathic urticaria was associated with positive serum IgG for H. Pylori. A trial of H. pylori eradication therapy may be offered to patients with CIU and evidence of H. pylori infection. Journal of Armed Forces Medical College Bangladesh Vol.12(2) 2016: 122-126

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Prevalence of Helicobacter Pylori Infection and Associated Factors Among Gastritis Patents in Yekatit 12 Teaching Hospital Addis Ababa Ethiopia

10.21203/rs.3.rs-114088/v1 ◽

2020 ◽

Author(s):

Awraris Hailu ◽

Bekureamanuel Sileshi ◽

Hazaratali Panari

Keyword(s):

Helicobacter Pylori ◽

Teaching Hospital ◽

Rural Areas ◽

Associated Factors ◽

Addis Ababa ◽

Helicobacter Pylori Infection ◽

Work Place ◽

P Value ◽

Hand Wash ◽

H Pylori

Abstract Introduction: Helicobacter pylori colonize the stomach of about ~50% of the world’s human population and infection is more in clients with dyspeptic conditions and its associated with the severity of gastritis. The present study revealed that the magnitude of H. Pylori and burden of the bacterial infection as well as the contribution of H. Pylori for gastritis and also assesses the current prevalence of H. Pylori infection.Objective: To evaluate the prevalence of Helicobacter Pylori infection and associated factors among gastritis patents in Yekatit 12 Hospital.Methodology: An institutional based cross-sectional study was conducted in Yekatit 12 teaching hospital Addis Ababa city, Ethiopia. The participants were those patients come to the hospital for treatment of gastritis and send to the laboratory to confirm Helicobacter pylori infection in the data collection period. A total of 394 participants were interviewed by using a structured pre tested questionnaire. The data were coded and entered into Epi Data 3.1 version , cleaned and exported to version 20, SPSS. Multiple Logistic regression was used to estimate Adjusted (AORs) with a 95% confidence interval (CI) of positive responses to the different risk factors. P-value < 0.05 was considered as significant.Result: The total occurrence of H. Pylori in observers was 25.9%. Regarding income of family, those participants who have less income and living in rural areas were extra prone to be infected with H. Pylori (AOR=5.857, CI 95% = 1.389-24.686, P = 0.016 and AOR=3.663, CI 95% = 1.068-12.557, P =0.039) respectively. Further, the prevalence of study was significant association with participants who had experience of gastrointestinal illness, mouth to mouth kissing, unable to hand wash regularly before meal and after latrine used (AOR=4.270, 95%CI=1.785-10.21, P=0.01, AOR=53.085, 95%CI=16.185-174.114, P=0.000, AOR=7.316, 95%CI=1.944-27.536, P=0.003, AOR=3.374, 95%CI=1.024-11.114, P=0.046) respectively.Conclusion and recommendation: The finding shows that H. Pylori infection was significantly associated with occupational status,gender, smoking tobacco and hand washing regularly before meal and after toilet. I recommend making clean and saving the work place; regular hand wash, before food preparation, before feeding and after latrine. A health professional should give health education by using different media about the transition and health biro also should give emphases.

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