scholarly journals Use of adipose stem cells and polylactide discs for tissue engineering of the temporomandibular joint disc

2009 ◽  
Vol 7 (42) ◽  
pp. 177-188 ◽  
Author(s):  
Katja Mäenpää ◽  
Ville Ellä ◽  
Jari Mauno ◽  
Minna Kellomäki ◽  
Riitta Suuronen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Temporomandibular Joint ◽  
Adipose Stem Cells ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Type I ◽  
Mrna Levels ◽  
Chondrogenic Medium ◽  
Tmj Disc

There is currently no suitable replacement for damaged temporomandibular joint (TMJ) discs after discectomy. In the present study, we fabricated bilayer biodegradable polylactide (PLA) discs comprising a non-woven mat of poly(L/D)lactide (P(L/D)LA) 96/4 and a P(L/DL)LA 70/30 membrane plate. The PLA disc was examined in combination with adipose stem cells (ASCs) for tissue engineering of the fibrocartilaginous TMJ disc in vitro . ASCs were cultured in parallel in control and chondrogenic medium for a maximum of six weeks. Relative expression of the genes, aggrecan, type I collagen and type II collagen present in the TMJ disc extracellular matrix increased in the ASC-seeded PLA discs in the chondrogenic medium. The hypertrophic marker, type X collagen, was moderately induced. Alcian blue staining showed accumulation of sulphated glycosaminoglycans. ASC differentiation in the PLA discs was close to that observed in pellet cultures. Comparison of the mRNA levels revealed that the degree of ASC differentiation was lower than that in TMJ disc-derived cells and tissue. The pellet format supported the phenotype of the TMJ disc-derived cells under chondrogenic conditions and also enhanced their hyalinization potential, which is considered part of the TMJ disc degeneration process. Accordingly, the combination of ASCs and PLA discs has potential for the development of a tissue-engineered TMJ disc replacement.

scholarly journals Silencing Smad7 Potentiates BMP2-induced Chondrogenic Differentiation and Inhibits Endochondral Ossification in Human Synovial Derived Mesenchymal Stem Cells

2020 ◽  
Author(s):  
pengcheng xiao ◽  
Zhenglin Zhu ◽  
Chengcheng Du ◽  
Yongsheng Zeng ◽  
junyi Liao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Endochondral Ossification ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Cartilage Injuries

Abstract Background: Cartilage injuries pose formidable challenges for effective clinical management. Autologous stem cell-based therapies and transgene-enhanced cartilage tissue engineering may open new avenues for the treatment of cartilage injuries. Bone morphogenetic protein 2 (BMP2) is a promising chondrogenic growth factors for transgene-enhanced cartilage tissue engineering. However the BMP2 is failed to maintain a stable chondrogenic phenotype as it also induces robust endochondral ossification. Recently, human synovial derived mesenchymal stem cells (hSMSCs) arouse interested through the poor differentiation potential into osteogenic lineage. Smad7, a protein to antagonizes TGF-β/BMP signaling pathway has been discovered significant in the endochondral ossification. In the present study ,we further explore the effect of downregulate Smad7 in BMP2-induced chondrogenic differentiation of hSMSCs. Methods: hSMSCs were isolated from synovium of human knee joint through adhesion growth. In vitro and in vivo chondrogenic differentiation models of hSMSCs were constructed . Transgenes of BMP2, silencing Smad7 and Smad7 were expressed by adenoviral vectors. The osteogenic differentiation was detected by alkaline phosphatase staining, alizarin red staining. The chondrogenic differentiation was detected by alcian blue staining. Gene expression was determined by reverse transcription and quantitative real-time PCR (RT-qPCR), Immunofluorescence and immunohistochemistry. The subcutaneous stem cell implantation model was established and evaluated by micro-CT , h＆e staining, alcian blue staining and immunohistochemistry assay.Results: Compared to other MSCs, hSMSCs performed less of capability to osteogenic differentiation. But the occurrence of endochondral ossification is still inevasible during BMP2 induced cartilage formation. We found that silencing Smad7 enhanced the BMP2-induced chondrogenic differentiation of hSMSCs in vitro. Also, it leading to much less of hypertrophic differentiation. The subcutaneous stem cells implantation assays demonstrated silencing Smad7 potentiates BMP2-induced cartilage formation and inhibits endochondral ossification. Conclusion: This study strongly suggests that application of hSMSCs , cell scaffolds and silencing Smad7 can potentiate BMP2-induced chondrogenic differentiation and inhibit endochondral ossification. Thus, inhibit the expression of Smad7 in BMP2-induced hSMSCs differentiation may be a new strategy for cartilage tissue engineering.

scholarly journals Autologous adipose stem cells and polylactide discs in the replacement of the rabbit temporomandibular joint disc

2013 ◽  
Vol 10 (85) ◽  
pp. 20130287 ◽  
Author(s):  
Katja Ahtiainen ◽  
Jari Mauno ◽  
Ville Ellä ◽  
Jaana Hagström ◽  
Christian Lindqvist ◽  
...  
Keyword(s):  
Stem Cells ◽  
Temporomandibular Joint ◽  
Transforming Growth Factor ◽  
Morphological Changes ◽  
Adipose Stem Cells ◽  
Disc Replacement ◽  
Tmj Disc ◽  
The Right ◽  
Tissue Reactions ◽  
Tgf Β1

The temporomandibular joint (TMJ) disc lacks functional replacement after discectomy. We investigated tissue-engineered bilayer polylactide (PLA) discs and autologous adipose stem cells (ASCs) as a potential replacement for the TMJ disc. These ASC discs were pre-cultured either in control or in differentiation medium, including transforming growth factor (TGF)-β1 for one week. Prior to implantation, expression of fibrocartilaginous genes was measured by qRT-PCR. The control and differentiated ASC discs were implanted, respectively, in the right and left TMJs of rabbits for six ( n = 5) and 12 months ( n = 5). Thereafter, the excised TMJ areas were examined with cone beam computed tomography (CBCT) and histology. No signs of infection, inflammation or foreign body reactions were detected at histology, whereas chronic arthrosis and considerable condylar hypertrophy were observed in all operated joints at CBCT. The left condyle treated with the differentiated ASC discs appeared consistently smoother and more sclerotic than the right condyle. The ASC disc replacement resulted in dislocation and morphological changes in the rabbit TMJ. The ASC discs pre-treated with TGF-β1 enhanced the condylar integrity. While adverse tissue reactions were not shown, the authors suggest that with improved attachment and design, the PLA disc and biomaterial itself would hold potential for TMJ disc replacement.

scholarly journals Development of Synthetic and Natural Materials for Tissue Engineering Applications Using Adipose Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Yunfan He ◽  
Feng Lu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Tissue Regeneration ◽  
Large Scale ◽  
Clinical Applications ◽  
Adipose Stem Cells ◽  
Laboratory Research ◽  
Engineering Applications ◽  
Plastic And Reconstructive Surgery ◽  
Relative Ease

Adipose stem cells have prominent implications in tissue regeneration due to their abundance and relative ease of harvest from adipose tissue and their abilities to differentiate into mature cells of various tissue lineages and secrete various growth cytokines. Development of tissue engineering techniques in combination with various carrier scaffolds and adipose stem cells offers great potential in overcoming the existing limitations constraining classical approaches used in plastic and reconstructive surgery. However, as most tissue engineering techniques are new and highly experimental, there are still many practical challenges that must be overcome before laboratory research can lead to large-scale clinical applications. Tissue engineering is currently a growing field of medical research; in this review, we will discuss the progress in research on biomaterials and scaffolds for tissue engineering applications using adipose stem cells.

scholarly journals Combination of resveratrol-containing collagen with adipose stem cells for craniofacial tissue-engineering applications

2018 ◽  
Vol 15 (4) ◽  
pp. 660-672 ◽  
Author(s):  
Chih-Chien Wang ◽  
Chih-Hsin Wang ◽  
Hsiang-Cheng Chen ◽  
Juin-Hong Cherng ◽  
Shu-Jen Chang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Stem Cells ◽  
Engineering Applications ◽  
Craniofacial Tissue

scholarly journals Composite System of 3D-Printed Polymer and Acellular Matrix Hydrogel to Repair Temporomandibular Joint Disc

2021 ◽  
Vol 8 ◽  
Author(s):  
Ping Yi ◽  
Jiadi Liang ◽  
Futing Huang ◽  
Zuodong Zhao ◽  
Xiaohui Zhou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Temporomandibular Joint ◽  
Photoelectron Spectroscopy ◽  
Biomechanical Testing ◽  
Biomechanical Properties ◽  
Biological Properties ◽  
Cell Counting ◽  
Structural And Functional Properties ◽  
Tmj Disc ◽  
3D Printed

Tissue engineering is a promising approach to restore or replace a damaged temporomandibular joint (TMJ) disc. However, constructing a scaffold that can mimic biomechanical and biological properties of the natural TMJ disc remains a challenge. In this study, three-dimensional (3D) printing technology was used to fabricate polycaprolactone (PCL)/polyurethane (PU) scaffolds and PU scaffolds to imitate the region-specific biomechanical properties of the TMJ disc. The scaffolds were coated with polydopamine (PDA) and combined with a decellularized matrix (dECM). Then, rat costal chondrocytes and mouse L929 fibroblasts, respectively, were suspended on the composite scaffolds and the biological functions of the cells were studied. The properties of the scaffolds were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), contact angle analysis, and biomechanical testing. To verify the biocompatibility of the scaffolds, the viability, proliferation, and extracellular matrix (ECM) production of the cells seeded on the scaffolds were assessed by LIVE/DEAD staining, Cell Counting Kit-8 assay, biochemical content analysis, immunofluorescence staining, and qRT-PCR. The functionalized hybrid scaffolds were then implanted into the subcutaneous space of nude mice for 6 weeks, and the regenerated tissue was evaluated by histological staining. The biomechanical properties of PCL/PU and PU scaffolds were comparable to that of the central and peripheral zones, respectively, of a native human TMJ disc. The PDA-coated scaffolds displayed superior biomechanical, structural, and functional properties, creating a favorable microenvironment for cell survival, proliferation, ECM production, and tissue regeneration. In conclusion, 3D-printed polymer scaffolds coated with PDA and combined with dECM hydrogel were found to be a promising substitute for TMJ disc tissue engineering.

scholarly journals The Effect of Human Platelet-Rich Plasma and L-Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells

2019 ◽  
Vol 3 (1) ◽  
pp. 26 ◽  
Author(s):  
Imam Rosadi ◽  
Karina Karina ◽  
Iis Rosliana ◽  
Siti Sobariah ◽  
Irsyah Afini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ascorbic Acid ◽  
Alcian Blue ◽  
Human Platelet ◽  
Platelet Rich Plasma ◽  
Cartilage Tissue ◽  
Population Doubling ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Adipose Derived Stem Cells

Background: Microtia is a congenital malformation in the external ear due to cartilage defect. Adipose-derived stem cells (ADSC) is promising cells to develop cartilage tissue engineering for microtia. In this study, we focused on proliferation and chondrogenesis of ADSC in three different media, which consist of 10% fetal bovine serum (FBS), 10% FBS with L-ascorbic acid, and 10% human platelet rich plasma (PRP). Methods: ADSC were induced to differentiate into adipocytes, chondrocyte and osteocytes. ADSC morphology, proliferation and population doubling time was compared in three different media and analysed. Observation and alcian blue staining were done every 7 days to assess chondrogenic potency of ADSC from each treatment.Results: Isolated ADSC were able to differentiate into adipocytes, osteocytes and chondrocytes. ADSC in all group have fibroblast-like morphology, but cells in 10% FBS and 10% FBS with LAA group were flattened and larger. ADSC in 10% PRP group proliferates faster than 10% FBS with and without LAA. PDT values of ADSC were 34 hours, 44 hours and 48 hours, respectively for 10% PRP, 10% FBS with LAA and 10% FBS group. Alcian blue staining revealed that ADSC in 10% FBS with LAA and 10% PRP were able to proceed to chondrogenesis when cultured time were prolong up to 21 days, but not with ADSC in 10% FBS. Conclusion: We conclude that adding 10% FBS with LAA or 10% PRP into medium culture can support proliferation and chondrogenesis of ADSC. Key words: human ADSC, PRP, L-ascorbic acid, proliferation, chondrogenesis

scholarly journals Tissue engineering toward temporomandibular joint disc regeneration

2018 ◽  
Vol 10 (446) ◽  
pp. eaaq1802 ◽  
Author(s):  
Natalia Vapniarsky ◽  
Le W. Huwe ◽  
Boaz Arzi ◽  
Meghan K. Houghton ◽  
Mark E. Wong ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Temporomandibular Joint ◽  
Treated Group ◽  
Defect Closure ◽  
Repair Tissue ◽  
Temporomandibular Joint Disc ◽  
Disc Regeneration ◽  
Tmj Disc ◽  
Engineering Strategy

Treatments for temporomandibular joint (TMJ) disc thinning and perforation, conditions prevalent in TMJ pathologies, are palliative but not reparative. To address this, scaffold-free tissue-engineered implants were created using allogeneic, passaged costal chondrocytes. A combination of compressive and bioactive stimulation regimens produced implants with mechanical properties akin to those of the native disc. Efficacy in repairing disc thinning was examined in minipigs. Compared to empty controls, treatment with tissue-engineered implants restored disc integrity by inducing 4.4 times more complete defect closure, formed 3.4-fold stiffer repair tissue, and promoted 3.2-fold stiffer intralaminar fusion. The osteoarthritis score (indicative of degenerative changes) of the untreated group was 3.0-fold of the implant-treated group. This tissue engineering strategy paves the way for developing tissue-engineered implants as clinical treatments for TMJ disc thinning.

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