scholarly journals Thermovenabulum gondwanense sp. nov., a thermophilic anaerobic Fe(III)-reducing bacterium isolated from microbial mats thriving in a Great Artesian Basin bore runoff channel

2010 ◽  
Vol 60 (5) ◽  
pp. 1079-1084 ◽  
Author(s):  
Christopher D. Ogg ◽  
Anthony C. Greene ◽  
Bharat K. C. Patel
Keyword(s):  
Yeast Extract ◽  
Microbial Mats ◽  
Wide Spectrum ◽  
Thermal Waters ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Phylogenetic Distance ◽  
Artesian Basin ◽  
Great Artesian Basin ◽  
Casamino Acids

A strictly anaerobic, thermophilic bacterium, designated strain R270T, was isolated from microbial mats thriving in the thermal waters (66 °C) of a Great Artesian Basin bore (registered no. 17263) runoff channel. Cells of strain R270T were straight to slightly curved rods (3.50–6.00×0.75–1.00 μm) that stained Gram-positive, but possessed a Gram-negative cell-wall ultrastructure. Strain R270T grew optimally in tryptone-yeast extract-Casamino acids medium at 65 °C (growth temperature range between 50 and 70 °C) and at pH 7.0 (growth pH range between 6.0 and 9.0). In the presence of 0.02 and 0.10 % yeast extract, pyruvate and Casamino acids were the only substrates fermented from a wide spectrum of substrates tested. Fe(III), Mn(IV), thiosulfate and elemental sulfur were used as electron acceptors in the presence 0.2 % yeast extract, but not sulfate, sulfite, nitrate, nitrite or fumarate. Growth of strain R270T increased in the presence of Fe(III), which was reduced in the presence of peptone, tryptone, Casamino acids, amyl media, starch, pyruvate, H2 and CO2, but not in the presence of acetate, lactate, propionate, formate, benzoate, glycerol or ethanol. Growth and Fe(III) reduction were inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and 2 % NaCl (w/v). The DNA G+C content of strain R270T was 41±1 mol% (T m) and phylogenetic analysis of the 16S rRNA gene indicated that this isolate was closely related to Thermovenabulum ferriorganovorum DSM 14006T (similarity value of 96.1 %) within the family ‘Thermoanaerobacteraceae’, class ‘Clostridia’, phylum ‘Firmicutes’. On the basis of the phylogenetic distance separating the two, together with differences in a number of key phenotypic characteristics, strain R270T represents a novel species of the genus Thermovenabulum, for which the name Thermovenabulum gondwanense sp. nov. is proposed; the type strain is R270T (=KCTC 5616T=DSM 21133T).

scholarly journals Caloramator mitchellensis sp. nov., a thermoanaerobe isolated from the geothermal waters of the Great Artesian Basin of Australia, and emended description of the genus Caloramator

2011 ◽  
Vol 61 (3) ◽  
pp. 644-653 ◽  
Author(s):  
Christopher D. Ogg ◽  
Bharat K. C. Patel
Keyword(s):  
Yeast Extract ◽  
Phenotypic Traits ◽  
Rrna Gene ◽  
Geothermal Waters ◽  
Temperature Growth ◽  
Artesian Basin ◽  
Great Artesian Basin ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Casamino Acids

A strictly thermophilic anaerobe, designated strain VF08T, was isolated from a water sample collected from a Great Artesian Basin bore (registered bore number 22981) situated at Mitchell, QLD, Australia. Cells of isolate VF08T were slightly curved, non-sporulating rods (1.5–3.5×0.4–0.8 μm), which stained Gram-negative but possessed a Gram-positive cell-wall ultrastructure. The strain grew optimally in tryptone-yeast extract-glucose (TYEG) medium at 55 °C (temperature growth range between 37 and 60 °C) and a pH of 7 (pH growth range, 6.0–9.0). Yeast extract or tryptone was required for growth on glucose, fructose, xylose, maltose, sucrose, raffinose, cellobiose, ribose, pyruvate, tryptone, peptone, Casamino acids, amyl media and serine, but could also support growth as the sole carbon source. End products from glucose fermentation were acetate, ethanol, CO2 and H2. The strain reduced vanadium(V), but not iron(III), manganese(IV), elemental sulfur, sulfate, thiosulfate, sulfite, nitrate or nitrite in the presence of 0.2 % yeast extract, peptone, tryptone, glucose, sucrose and Casamino acids, but an increase in the growth rate or cell yield was not observed. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and ≥2 % NaCl (w/v). The G+C content of the DNA was 38.4±0.8 mol% as determined by the thermal denaturation (T m) method. 16S rRNA gene sequence analysis revealed that isolate VF08T was a member of the genus Caloramator with Caloramator australicus and Caloramator fervidus (formerly Clostridium fervidus) being the closest relatives with similarity values of 85.0 and 86.1 %, respectively, when helix 6 nucleotides were included in the analysis, and 95.2 % and 94 %, respectively, when these nucleotides were masked from the analysis. Further analysis revealed that strain VF08T formed an individual cluster (cluster II) within the genus Caloramator and could be distinguished from other species within the genus Caloramator (clusters I, III and IV) on the basis of signature nucleotides and differences in phenotypic traits. These data suggest that strain VF08T is a novel species of the genus Caloramator, for which the name Caloramator mitchellensis sp. nov. is proposed. The type strain is VF08T (=JCM 15828T=KCTC 5735T). An emended description of the genus Caloramator is also provided.

scholarly journals Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters

2010 ◽  
Vol 60 (6) ◽  
pp. 1394-1400 ◽  
Author(s):  
Christopher D. Ogg ◽  
Bharat K. C. Patel
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Yeast Extract ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Rrna Gene Sequence ◽  
Casamino Acids ◽  
Gene Similarity

A strictly anaerobic, thermophilic bacterium, designated strain AeBT, was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeBT were slightly curved rods (2.5–6.0×1.0 μm) that stained Gram-negative and formed spherical terminal to subterminal spores. The strain grew optimally in tryptone–yeast extract–Casamino acids medium at 50 °C (range 37–55 °C) and pH 7 (range pH 5–9). Strain AeBT grew poorly on yeast extract (0.2 %) and tryptone (0.2 %) as sole carbon sources, which were obligately required for growth on other energy sources. Growth of strain AeBT increased in the presence of various carbohydrates and amino acids, but not organic acids. End products detected from glucose fermentation were ethanol, acetate, CO2 and H2. In the presence of 0.2 % yeast extract, iron(III), manganese(IV), vanadium(V) and cobalt(III) were reduced, but not sulfate, thiosulfate, sulfite, elemental sulfur, nitrate or nitrite. Iron(III) was also reduced in the presence of tryptone, peptone, Casamino acids and amyl media (Research Achievement), but not starch, xylan, chitin, glycerol, ethanol, pyruvate, benzoate, lactate, acetate, propionate, succinate, glycine, serine, lysine, threonine, arginine, glutamate, valine, leucine, histidine, alanine, aspartate, isoleucine or methionine. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and NaCl concentrations >2 %. The DNA G+C content was 35.4±1 mol%, as determined by the thermal denaturation method. 16S rRNA gene sequence analysis indicated that strain AeBT is a member of the family Clostridiaceae, class Clostridia, phylum ‘Firmicutes’, and is positioned approximately equidistantly between the genera Sarcina, Anaerobacter, Caloramator and Clostridium (16S rRNA gene similarity values of 87.8–90.9 %). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain AeBT is considered to represent a novel species in a new genus, for which the name Fervidicella metallireducens gen. nov., sp. nov. is proposed; the type strain is AeBT (=JCM 15555T=KCTC 5667T).

Thermoanaerobacter pentosaceus sp. nov., an anaerobic, extremely thermophilic, high ethanol-yielding bacterium isolated from household waste

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2396-2404 ◽  
Author(s):  
Ana Faria Tomás ◽  
Dimitar Karakashev ◽  
Irini Angelidaki
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Maximum Yield ◽  
Maximum Growth ◽  
Household Waste ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Phylogenetic Distance ◽  
Content Type ◽  
Link Type

An extremely thermophilic, xylanolytic, spore-forming and strictly anaerobic bacterium, strain DTU01T, was isolated from a continuously stirred tank reactor fed with xylose and household waste. Cells stained Gram-negative and were rod-shaped (0.5–2 µm in length). Spores were terminal with a diameter of approximately 0.5 µm. Optimal growth occurred at 70 °C and pH 7, with a maximum growth rate of 0.1 h−1. DNA G+C content was 34.2 mol%. Strain DTU01T could ferment arabinose, cellobiose, fructose, galactose, glucose, lactose, mannitol, mannose, melibiose, pectin, starch, sucrose, xylan, yeast extract and xylose, but not cellulose, Avicel, inositol, inulin, glycerol, rhamnose, acetate, lactate, ethanol, butanol or peptone. Ethanol was the major fermentation product and a maximum yield of 1.39 mol ethanol per mol xylose was achieved when sulfite was added to the cultivation medium. Thiosulfate, but not sulfate, nitrate or nitrite, could be used as electron acceptor. On the basis of 16S rRNA gene sequence similarity, strain DTU01T was shown to be closely related to Thermoanaerobacter mathranii A3T, Thermoanaerobacter italicus Ab9T and Thermoanaerobacter thermocopriae JT3-3T, with 98–99 % similarity. Despite this, the physiological and phylogenetic differences (DNA G+C content, substrate utilization, electron acceptors, phylogenetic distance and isolation site) allow for the proposal of strain DTU01T as a representative of a novel species within the genus Thermoanaerobacter , for which the name Thermoanaerobacter pentosaceus sp. nov. is proposed, with the type strain DTU01T ( = DSM 25963T = KCTC 4529T = VKM B-2752T = CECT 8142T).

scholarly journals Acetobacteroides hydrogenigenes gen. nov., sp. nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 2986-2991 ◽  
Author(s):  
Xiao-Li Su ◽  
Qi Tian ◽  
Jie Zhang ◽  
Xian-Zheng Yuan ◽  
Xiao-Shuang Shi ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Type Species ◽  
Wastewater Sludge ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Sequence Similarities

A strictly anaerobic, mesophilic, carbohydrate-fermenting, hydrogen-producing bacterium, designated strain RL-CT, was isolated from a reed swamp in China. Cells were Gram-stain-negative, catalase-negative, non-spore-forming, non-motile rods measuring 0.7–1.0 µm in width and 3.0–8.0 µm in length. The optimum temperature for growth of strain RL-CT was 37 °C (range 25–40 °C) and pH 7.0–7.5 (range pH 5.7–8.0). The strain could grow fermentatively on yeast extract, tryptone, arabinose, glucose, galactose, mannose, maltose, lactose, glycogen, pectin and starch. The main end products of glucose fermentation were acetate, H2 and CO2. Organic acids, alcohols and amino acids were not utilized for growth. Yeast extract was not required for growth; however, it stimulated growth slightly. Nitrate, sulfate, sulfite, thiosulfate, elemental sulfur and Fe(III) nitrilotriacetate were not reduced as terminal electron acceptors. Aesculin was hydrolysed but not gelatin. Indole and H2S were produced from yeast extract. The G+C content of the genomic DNA was 51.2 mol%. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. The most abundant polar lipid of strain RL-CT was phosphatidylethanolamine. 16S rRNA gene sequence analysis revealed that the isolate belongs to the uncultured Blvii28 wastewater-sludge group (http://www.arb-silva.de/) in the family Rikenellaceae of the phylum Bacteroidetes, and shared low sequence similarities with the related species Alistipes shahii WAL 8301T (81.8 %), Rikenella microfusus ATCC 29728T (81.7 %) and Anaerocella delicata WN081T (80.9 %). On the basis of these data, a novel species in a new genus of the family Rikenellaceae is proposed, Acetobacteroides hydrogenigenes gen. nov., sp. nov. The type strain of the type species is RL-CT ( = JCM 17603T = DSM 24657T = CGMCC 1.5173T).

scholarly journals Thermococcus thioreducens sp. nov., a novel hyperthermophilic, obligately sulfur-reducing archaeon from a deep-sea hydrothermal vent

2007 ◽  
Vol 57 (7) ◽  
pp. 1612-1618 ◽  
Author(s):  
Elena V. Pikuta ◽  
Damien Marsic ◽  
Takashi Itoh ◽  
Asim K. Bej ◽  
Jane Tang ◽  
...  
Keyword(s):  
Hydrothermal Vent ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Black Smoker ◽  
Separate Species ◽  
Gene Sequence Analysis ◽  
Nacl Concentration ◽  
Mid Atlantic Ridge ◽  
Casamino Acids ◽  
Ph Range

A hyperthermophilic, sulfur-reducing, organo-heterotrophic archaeon, strain OGL-20PT, was isolated from ‘black smoker’ chimney material from the Rainbow hydrothermal vent site on the Mid-Atlantic Ridge (36.2°N, 33.9°W). The cells of strain OGL-20PT have an irregular coccoid shape and are motile with a single flagellum. Growth was observed within a pH range of 5.0−8.5 (optimum pH 7.0), an NaCl concentration range of 1–5 % (w/v) (optimum 3 %) and a temperature range of 55–94 °C (optimum 83–85 °C). The novel isolate is strictly anaerobic and obligately dependent upon elemental sulfur as an electron acceptor, but it does not reduce sulfate, sulfite, thiosulfate, Fe(III) or nitrate. Proteolysis products (peptone, bacto-tryptone, Casamino acids and yeast extract) are utilized as substrates during sulfur reduction. Strain OGL-20PT is resistant to ampicillin, chloramphenicol, kanamycin and gentamicin, but sensitive to tetracycline and rifampicin. The G+C content of the DNA is 52.9 mol%. The 16S rRNA gene sequence analysis revealed that strain OGL-20PT is closely related to Thermococcus coalescens and related species, but no significant homology by DNA–DNA hybridization was observed between those species and the new isolate. On the basis of physiological and molecular properties of the new isolate, we conclude that strain OGL-20PT represents a new separate species within the genus Thermococcus, for which we propose the name Thermococcus thioreducens sp. nov. The type strain is OGL-20PT (=JCM 12859T=DSM 14981T=ATCC BAA-394T).

scholarly journals Bellilinea caldifistulae gen. nov., sp. nov. and Longilinea arvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading consortia

2007 ◽  
Vol 57 (10) ◽  
pp. 2299-2306 ◽  
Author(s):  
Takeshi Yamada ◽  
Hiroyuki Imachi ◽  
Akiyoshi Ohashi ◽  
Hideki Harada ◽  
Satoshi Hanada ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Rice Paddy ◽  
Limited Range ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Optimum Growth ◽  
Cell Width ◽  
Genetic Traits ◽  
Ph Range ◽  
Micro Organisms

Thermophilic (strain GOMI-1T) and mesophilic (strain KOME-1T) strains were isolated from two different cultures of propionate-degrading consortia obtained from thermophilic digester sludge and rice paddy soil, respectively. The two strains were non-spore-forming, non-motile and Gram-negative. Both strains were obligately anaerobic micro-organisms, showing multicellular filamentous morphotypes more than 100 μm in length. The cell width for strain GOMI-1T was 0.2–0.4 μm and that of strain KOME-1T was 0.4–0.6 μm. Strain GOMI-1T could grow at 45–65 °C with a pH range of 6.0–7.5 (optimum growth at 55 °C, pH 7.0). The temperature range for growth of strain KOME-1T was 30–40 °C and the pH range was pH 5.0–8.5 (optimum growth around 37 °C, pH 7.0). Yeast extract was required for growth of both strains. Strain GOMI-1T was able to grow with a number of carbohydrates in the presence of yeast extract. In yeast extract-containing medium, strain KOME-1T could utilize proteins and a limited range of sugars for growth. The G+C contents of the DNA of strains GOMI-1T and KOME-1T were respectively 54.7 and 57.6 mol%. Major fatty acids of strain GOMI-1T were C16 : 0, C14 : 0 and iso-C15 : 0, whereas those of strain KOME-1T were iso-C15 : 0, anteiso-C15 : 0 and C14 : 0. Based on comparative analysis of 16S rRNA gene sequences of strains GOMI-1T and KOME-1T, the strains were placed in different phylogenetic positions in the class Anaerolineae of the bacterial phylum Chloroflexi. Their phenotypic and genetic traits strongly supported the conclusion that the strains should be described as two independent taxa in the class Anaerolineae. Hence, we propose the names Bellilinea caldifistulae gen. nov., sp. nov., and Longilinea arvoryzae gen. nov., sp. nov., for strains GOMI-1T and KOME-1T. The type strains of Bellilinea caldifistulae and Longilinea arvoryzae are respectively GOMI-1T (=JCM 13669T =DSM 17877T) and KOME-1T (=JCM 13670T =KTCC 5380T).

scholarly journals Alkaliphilus halophilus sp. nov., a strictly anaerobic and halophilic bacterium isolated from a saline lake, and emended description of the genus Alkaliphilus

2010 ◽  
Vol 60 (12) ◽  
pp. 2898-2902 ◽  
Author(s):  
Xiao-Yue Wu ◽  
Kong-Lan Shi ◽  
Xue-Wei Xu ◽  
Min Wu ◽  
Aharon Oren ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Saline Lake ◽  
Salt Lake ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Fermentation Products ◽  
Substrate Utilization Pattern ◽  
Emended Description ◽  
Casamino Acids

A novel strictly anaerobic, halophilic and fermentative strain, designated E2RT, was isolated from sediments of Xiaokule salt lake in Xinjiang Province, China. Cells were straight to slightly curved, Gram-stain-positive rods that were motile by means of flagella and formed endospores. Strain E2RT was moderately halophilic and grew optimally in the presence of 7.5 % NaCl, at pH 8.0 and at 32 °C. Substrates used include yeast extract, Casamino acids, tryptone, fructose, sucrose, xylose, ribose, lactate and tartrate. Thiosulfate could be used as an accessory electron acceptor and stimulated growth. The main fermentation products from fructose were formate and acetate. The predominant fatty acids were iso-C15 : 0, iso-C15 : 1 F and iso-C13 : 0. 16S rRNA gene sequence analyses revealed that strain E2RT was related most closely to members of the genus Alkaliphilus (95.5–91.1 % similarity). The G+C content of strain E2RT was 28.5 mol%. Strain E2RT could be differentiated from its closest relatives based on its halophilic nature and its lower DNA G+C content. It could also be differentiated based on its substrate utilization pattern and relatively high levels of iso-C15 : 0. On the basis of these data, strain E2RT is considered to represent a novel species of the genus Alkaliphilus, for which the name Alkaliphilus halophilus sp. nov. is proposed. The type strain is E2RT (=CGMCC 1.5124T =JCM 16124T). An emended description of the genus Alkaliphilus is also provided.

scholarly journals Sporosalibacterium faouarense gen. nov., sp. nov., a moderately halophilic bacterium isolated from oil-contaminated soil

2011 ◽  
Vol 61 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Raja Rezgui ◽  
Zouhaier Ben Ali Gam ◽  
Said Ben Hamed ◽  
Marie-Laure Fardeau ◽  
Jean-Luc Cayol ◽  
...  
Keyword(s):  
Cell Wall ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Yeast Extract ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Gram Positive ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
The Family

A novel strictly anaerobic, moderately halophilic and mesophilic bacterium, designated strain SOL3f37T, was isolated from a hydrocarbon-polluted soil surrounding a deep petroleum environment located in south Tunisia. Cells of strain SOL3f37T stained Gram-positive and were motile, straight and spore-forming. Strain SOL3f37T had a typical Gram-positive-type cell-wall structure, unlike the thick, multilayered cell wall of its closest relative Clostridiisalibacter paucivorans. The major fatty acids were iso-C15 : 0 (41 %), iso-C14 : 0 3-OH and/or iso-C15 : 0 dimethyl acetal (21.6 %), iso-C13 : 0 (4.4 %), anteiso-C15 : 0 (3.9 %) and iso-C15 : 1 (2.8 %). Strain SOL3f37T grew between 20 and 48 °C (optimum 40 °C) and at pH 6.2–8.1 (optimum pH 6.9). Strain SOL3f37T required at least 0.5 g NaCl l−1 and grew in the presence of NaCl concentrations up to 150 g l−1 (optimum 40 g l−1). Yeast extract (2 g l−1) was required for degradation of pyruvate, fumarate, fructose, glucose and mannitol. Also, strain SOL3f37T grew heterotrophically on yeast extract, peptone and bio-Trypticase, but was unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content was 30.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SOL3f37T was a member of the family Clostridiaceae in the order Clostridiales; strain SOL3f37T was related to members of various genera of the family Clostridiaceae. It exhibited highest 16S rRNA gene sequence similarity (93.4 %) with Clostridiisalibacter paucivorans 37HS60T, 91.8 % with Thermohalobacter berrensis CTT3T and 91.7 % with Caloranaerobacter azorensis MV1087T. On the basis of genotypic, phenotypic and phylogenetic data, it is suggested that strain SOL3f37T represents a novel species in a new genus. The name Sporosalibacterium faouarense gen. nov., sp. nov. is proposed, with SOL3f37T (=DSM 21485T =JCM 15487T) as the type strain of Sporosalibacterium faouarense.

scholarly journals Draft Genome Sequence of Caloramator mitchellensis , a Thermoanaerobe Isolated from the Waters of the Great Artesian Basin

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Bharat K. C. Patel ◽  
Valentino Setoa Junior Te'o
Keyword(s):  
Genome Sequence ◽  
Anaerobic Bacterium ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Phenotypic Traits ◽  
Strictly Anaerobic ◽  
Artesian Basin ◽  
Great Artesian Basin ◽  
Flowing Waters ◽  
Bore Well

The genome sequence of Caloramator mitchellensis strain VF08, a rod-shaped, heterotrophic, strictly anaerobic bacterium isolated from the free-flowing waters of a Great Artesian Basin (GAB) bore well located in Mitchell, an outback Queensland town in Australia, is reported here. The analysis of the 2.42-Mb genome sequence indicates that the attributes of the genome are consistent with its physiological and phenotypic traits.

Palaeococcus pacificus sp. nov., an archaeon from deep-sea hydrothermal sediment

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2155-2159 ◽  
Author(s):  
Xiang Zeng ◽  
Xiaobo Zhang ◽  
Lijing Jiang ◽  
Karine Alain ◽  
Mohamed Jebbar ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Elemental Sulfur ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Physiological Properties ◽  
Casamino Acids ◽  
Gene Similarity

A hyperthermophilic, anaerobic, piezophilic archaeon (strain DY20341T) was isolated from a sediment sample collected from an East Pacific Ocean hydrothermal field (1° 37′ S 102° 45′ W) at a depth of 2737 m. The cells were irregular cocci, 0.8–1.5 µm in diameter. Growth was observed between 50 and 90 °C (optimum 80 °C), pH 5.0 and 8.0 (optimum pH 7.0), 1 % and 7 % (w/v) sea salts (Sigma, optimum 3 %), 1 % and 4 % (w/v) NaCl (optimum 3 %) and 0.1 and 80 MPa (optimum 30 MPa). The minimum doubling time was 66 min at 30 MPa and 80 °C. The isolate was an obligate chemoorganoheterotroph, capable of utilizing complex organic compounds and organic acids including yeast extract, peptone, tryptone, casein, starch, Casamino acids, citrate, lactate, acetate, fumarate, propanoate and pyruvate for growth. It was strictly anaerobic and facultatively dependent on elemental sulfur or sulfate as electron acceptors, but did not reduce sulfite, thiosulfate, Fe(III) or nitrate. The presence of elemental sulfur enhanced growth. The G+C content of the genomic DNA was 43.6±1 mol%. 16S rRNA gene sequence analysis revealed that the closest relative of the isolated organism was Palaeococcus ferrophilus DMJT (95.7 % 16S rRNA gene similarity). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name Palaeococcus pacificus sp. nov. is proposed. The type strain is strain DY20341T ( = JCM 17873T = DSM 24777T).

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