scholarly journals Mycobacterium parmense sp. nov.

2004 ◽  
Vol 54 (4) ◽  
pp. 1123-1127 ◽  
Author(s):  
Franco Fanti ◽  
Enrico Tortoli ◽  
Leslie Hall ◽  
Glenn D. Roberts ◽  
Reiner M. Kroppenstedt ◽  
...  
Keyword(s):  
Lymph Node ◽  
Type Strain ◽  
Cervical Lymph Node ◽  
16S Rdna Sequencing ◽  
Mycobacterial Species ◽  
Isolation And Identification ◽  
Rdna Sequencing ◽  
Lipid Pattern ◽  
Slow Growing ◽  
Mycobacterium Simiae

The isolation and identification of a novel, slow-growing, scotochromogenic, mycobacterial species is reported. A strain, designated MUP 1182T, was isolated from a cervical lymph node of a 3-year-old child. MUP 1182T is alcohol- and acid-fast, with a lipid pattern that is consistent with those of species that belong to the genus Mycobacterium. It grows slowly at 25–37 °C, but does not grow at 42 °C. The isolate was revealed to be biochemically distinct from previously described mycobacterial species: it has urease and Tween hydrolysis activities and lacks nitrate reductase, 3-day arylsulfatase and β-glucosidase activities. Comparative 16S rDNA sequencing showed that isolate MUP 1182T represents a novel, slow-growing species that is related closely to Mycobacterium lentiflavum and Mycobacterium simiae. On the basis of these findings, the name Mycobacterium parmense sp. nov. is proposed, with MUP 1182T (=CIP 107385T=DSM 44553T) as the type strain.

scholarly journals Isolation and identification of guaiacol producing Alicyclobacillus fastidiosus strains from orchards in Poland

2021 ◽  
Author(s):  
Barbara Sokolowska ◽  
Marzena Połaska ◽  
Agnieszka Dekowska
Keyword(s):  
Hot Springs ◽  
Biochemical Properties ◽  
Flavor Compounds ◽  
Natural Environments ◽  
16S Rdna Sequencing ◽  
Isolation And Identification ◽  
Acidophilic Bacteria ◽  
Rdna Sequencing ◽  
Spoilage Bacteria ◽  
Orchard Soils

The genus Alicyclobacillus comprises a group of Gram-positive, thermo-acidophilic bacteria that are capable of producing highly resistant endospores during unfavorable environmental conditions. The members of this genus inhabit natural environments, including hot springs and soils. The main reason behind the spoilage of final commercial fruit products by Alicyclobacillus is the contamination of fruits with soil at the time of harvesting. Some of the Alicyclobacillus species, including Alicyclobacillus acidoterrestris, are categorized as spoilage bacteria due to their ability to produce off-flavor compounds (e.g., guaiacol and halophenols) that adversely affect the taste and aroma of beverages. In our study, Alicyclobacillus species were isolated from Polish orchard soils and fruits and were subjected to 16S rDNA sequencing. The results of the analysis showed that the isolated strains belonged to A. acidoterrestris and Alicyclobacillus fastidiosus species. All the three isolated strains of A. fastidiosus (f1, f2, f3) exhibited similar morphological and biochemical properties as the strain described in the literature. However, these isolated strains were able to produce guaiacol at temperatures of 20°C, 25°C, and 45°C. Thus, the strains of A. fastidiosus discovered in the present study can be included in the group of spoilage species as they possessed the gene responsible for the production of guaiacol.

scholarly journals Cervical lymph node metastasis revealing a prostate adenocarcinoma: About two observations and Review of the Literature

2021 ◽  
pp. 27-34
Author(s):  
Sylvain DIembi ◽  
Boris Harold Otouana Dzon ◽  
Gérard Chidrel Ngouoni ◽  
Franck Arnaud Itiere OdzilI ◽  
Synclair Ngotene Diembi ◽  
...  
Keyword(s):  
Lymph Node ◽  
Cervical Lymph Node ◽  
Cervical Lymph Node Metastasis ◽  
Prostate Adenocarcinoma ◽  
Review Of The Literature ◽  
Node Metastasis ◽  
Common Cancer ◽  
Hormone Sensitive ◽  
Node Metastases ◽  
Slow Growing

Prostatic adenocarcinoma is the most common cancer in men, and is the 3rd leading cause of death in men worldwide. This slow-growing, hormone-sensitive cancer occurs after the age of 50 years. Cervical lymph node metastases of prostate adenocarcinoma are rare. We report two anatomical observations over a ten-year period of activity. The two men were aged 80 and 72 years, respectively, and both presented with multiple cervical adenopathies of particular topography above the clavicle, associated with urinary signs such as dysuria, pollakiuria and incontinence. Microscopic examination coupled with immunohistochemistry (anti-PSA antibodies) of the excised biopsy of these lymph nodes contributed to the diagnosis of certainty. Keywords: Adenocarcinoma; Prostate; Metastasis; Node; Neck; Immunohistochemistry

scholarly journals Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov.

2004 ◽  
Vol 54 (2) ◽  
pp. 583-592 ◽  
Author(s):  
Emenike R. K. Eribe ◽  
Bruce J. Paster ◽  
Dominique A. Caugant ◽  
Floyd E. Dewhirst ◽  
Verlyn K. Stromberg ◽  
...  
Keyword(s):  
16S Rdna ◽  
Type Strain ◽  
Light And Electron Microscopy ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
16S Rdna Sequencing ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Rdna Sequencing ◽  
Sds Page

Sixty strains of Gram-negative, anaerobic, rod-shaped bacteria from human sources initially assigned to Leptotrichia buccalis (n=58) and ‘Leptotrichia pseudobuccalis’ (n=2) have been subjected to polyphasic taxonomy. Full-length 16S rDNA sequencing, DNA–DNA hybridization, RAPD, SDS-PAGE of whole-cell proteins, cellular fatty acid analysis and enzymic/biochemical tests supported the establishment of four novel Leptotrichia species from this collection, Leptotrichia goodfellowii sp. nov. (type strain LB 57T=CCUG 32286T=CIP 107915T), Leptotrichia hofstadii sp. nov. (type strain LB 23T=CCUG 47504T=CIP 107917T), Leptotrichia shahii sp. nov. (type strain LB 37T=CCUG 47503T=CIP 107916T) and Leptotrichia wadei sp. nov. (type strain LB 16T=CCUG 47505T=CIP 107918T). Light and electron microscopy showed that the four novel species were Gram-negative, non-spore-forming and non-motile rods. L. goodfellowii produced arginine dihydrolase, β-galactosidase, N-acetyl-β-glucosaminidase, arginine arylamidase, leucine arylamidase and histidine arylamidase. L. shahii produced α-arabinosidase. L. buccalis and L. goodfellowii fermented mannose and were β-galactosidase-6-phosphate positive. L. goodfellowii, L. hofstadii and L. wadei were β-haemolytic. L. buccalis fermented raffinose. With L. buccalis, L. goodfellowii showed 3·8–5·5 % DNA–DNA relatedness, L. shahii showed 24·5–34·1 % relatedness, L. hofstadii showed 27·3–36·3 % relatedness and L. wadei showed 24·1–35·9 % relatedness. 16S rDNA sequencing demonstrated that L. hofstadii, L. shahii, L. wadei and L. goodfellowii each formed individual clusters with 97, 96, 94 and 92 % similarity, respectively, to L. buccalis.

scholarly journals Hidden Microcosmos in Slovak Gold Mine Rozalia – Microbial Gold Miners?

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Lenka MALINIČOVÁ ◽  
Lea NOSÁĽOVÁ ◽  
Ivana TIMKOVÁ ◽  
Peter PRISTAŠ ◽  
Jana SEDLÁKOVÁ-KADUKOVÁ
Keyword(s):  
Protein Profiling ◽  
Gold Mine ◽  
16S Rdna Sequencing ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Physical Chemical ◽  
Rdna Sequencing ◽  
Gold Miners ◽  
Processing Techniques ◽  
Tof Ms

Biogeochemical cycling of gold involves dispersion and reconcentration of gold (Au) due to physical, chemical and biological processesin Earth surface environments. These processes are evocated by a metabolic activity of different microbial taxa but many of them (andalso their biogeochemical potential) are still unexplored. Understanding the gold cycling is necessary for developing innovative, environmentally friendly gold processing techniques. Our experiments were aimed on isolation and identification of heterotrophic bacteriafrom ore and ore storage dump samples collected in Rozália gold mine in Hodruša-Hámre. Using culture-based approach followed bycombination of MALDI-TOF MS protein profiling and 16S rDNA sequencing, 18 different bacterial genera were identified in studiedmicrobiota. The participation of several representatives of these genera in individual gold cycling steps has already been reported. Thereal involvement of bacterial isolates in gold transformation reactions and their biogeochemical potential will be studied in subsequentexperiments.

scholarly journals Complete Genome Sequence of Mycobacterium heckeshornense JCM 15655T, Closely Related to a Pathogenic Nontuberculous Mycobacterial Species, Mycobacterium xenopi

2021 ◽  
Vol 10 (10) ◽  
Author(s):  
Mitsunori Yoshida ◽  
Hanako Fukano ◽  
Takanori Asakura ◽  
Masato Suzuki ◽  
Yoshihiko Hoshino
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Mycobacterial Species ◽  
Essential Information ◽  
Comparative Genome ◽  
Content Type ◽  
Mycobacterium Xenopi ◽  
Slow Growing

ABSTRACT Mycobacterium heckeshornense is a slow-growing mycobacterial species for which pathogenic features are unclear. Here, we report the complete genome sequence of an M. heckeshornense type strain. This sequence will provide essential information for future taxonomic and comparative genome studies of these mycobacteria.

Role of Clinical Palpation in Evaluation of Cervical Lymph Node in Oral Squamous Cell Carcinoma

2013 ◽  
Vol 1 (2) ◽  
pp. 02-06
Author(s):  
SM Anwar Sadat ◽  
Sufia Nasrin Rita ◽  
Shoma Banik ◽  
Md Nazmul Hasan Khandker ◽  
Md Mahfuz Hossain ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Oral Squamous Cell Carcinoma ◽  
Lymph Nodes ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Predictive Value ◽  
Cervical Lymph Node ◽  
Betel Quid ◽  
Cervical Lymph Nodes

A cross sectional study of 29 cases of oral squamous cell carcinoma with or without  cervical lymph node metastasis was done among Bangladeshi patients from January 2006 to December 2007. Majority of the study subjects (34.5%) belonged to the age group of 40-49 years. 58.6% of the study subjects were male, while remaining 41.4% of them were female. 51.7% of the lesions were located in the alveolar ridge where the other common sites were buccal mucosa (27.6%) and retro molar area (13.8%). Half of the study subjects (51.7%) were habituated to betel quid chewing followed by 37.9% and 10.3% were habituated to smoking and betel quid-smoking respectively. Grade I lesions was most prevalent (75.9%) in the study subjects.  Majority of cases presented with Stage IV lesions (55.2%). The sensitivity, specificity, positive predictive value, negative predictive value & accuracy of clinical palpation method for determining metastatic cervical lymph nodes were 93.33%, 64.29%, 73.68%, 90% and 79.3% respectively. Careful and repeated clinical palpation plays important role in evaluation of cervical lymph nodes though several modern techniques may help additionally in the management of oral cancer.DOI: http://dx.doi.org/10.3329/updcj.v1i2.13978 Update Dent. Coll. j. 2011: 1(2): 02-06

Cervical lymph node pathology on lymphatic enhanced CT scan

1988 ◽  
Vol 24 (6) ◽  
pp. 994
Author(s):  
T S Chung ◽  
K M Kim ◽  
J H Suh ◽  
D I Kim ◽  
H J Jeong
Keyword(s):  
Lymph Node ◽  
Ct Scan ◽  
Cervical Lymph Node ◽  
Enhanced Ct ◽  
Lymph Node Pathology

Cervical lymph node metastasis in thyroid microcarcinoma

2019 ◽  
Author(s):  
Mouna Bellakhdhar ◽  
Jihene Houas ◽  
Monia Ghammem ◽  
Abir Meherzi ◽  
Wassim Kermani ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cervical Lymph Node ◽  
Cervical Lymph Node Metastasis ◽  
Node Metastasis ◽  
Thyroid Microcarcinoma
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