Life-threatening Escherichia coli cellulitis in patients with haematological malignancies

2012 ◽  
Vol 61 (9) ◽  
pp. 1324-1327 ◽  
Author(s):  
S. Sunder ◽  
E. Haguenoer ◽  
D. Bouvet ◽  
S. Lissandre ◽  
A. Bree ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Haematological Malignancies ◽  
Life Threatening

scholarly journals Escherichia coli bacteraemia in pregnant women is life-threatening for foetuses

2014 ◽  
Vol 20 (12) ◽  
pp. O1035-O1041 ◽  
Author(s):  
L. Surgers ◽  
A. Bleibtreu ◽  
C. Burdet ◽  
O. Clermont ◽  
C. Laouénan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pregnant Women ◽  
Life Threatening

scholarly journals Nitrofurantoin Combined With Amikacin: A Promising Alternative Strategy for Combating MDR Uropathogenic Escherichia coli

2020 ◽  
Vol 10 ◽  
Author(s):  
Zi-Xing Zhong ◽  
Ze-Hua Cui ◽  
Xiao-Jie Li ◽  
Tian Tang ◽  
Zi-Jian Zheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Resistance ◽  
Galleria Mellonella ◽  
Synergistic Effects ◽  
Promising Alternative ◽  
Poor Patient Compliance ◽  
Life Threatening ◽  
Tract Infections ◽  
Time Kill

Urinary tract infections (UTI) are common infections that can be mild to life threatening. However, increased bacterial resistance and poor patient compliance rates have limited the effectiveness of conventional antibiotic therapies. Here, we investigated the relationship between nitrofurantoin and amikacin against 12 clinical MDR uropathogenic Escherichia coli (UPEC) strains both in vitro and in an experimental Galleria mellonella model. In vitro synergistic effects were observed in all 12 test strains by standard checkerboard and time-kill assays. Importantly, amikacin or nitrofurantoin at half of the clinical doses were not effective in the treatment of UPEC infections in the G. mellonella model but the combination therapy significantly increased G. mellonella survival from infections caused by all 12 study UPEC strains. Taken together, these results demonstrated synergy effects between nitrofurantoin and amikacin against MDR UPEC.

Prophylactic and interventional granulocyte transfusions in patients with haematological malignancies and life-threatening infections during neutropenia

2005 ◽  
Vol 84 (11) ◽  
pp. 734-741 ◽  
Author(s):  
Sabine Mousset ◽  
Stella Hermann ◽  
Stefan A. Klein ◽  
Heike Bialleck ◽  
Michaele Duchscherer ◽  
...  
Keyword(s):  
Haematological Malignancies ◽  
Life Threatening

scholarly journals Role of Escherichia coli O157:H7 Virulence Factors in Colonization at the Bovine Terminal Rectal Mucosa

2006 ◽  
Vol 74 (8) ◽  
pp. 4685-4693 ◽  
Author(s):  
Haiqing Sheng ◽  
Ji Youn Lim ◽  
Hannah J. Knecht ◽  
Jie Li ◽  
Carolyn J. Hovde
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Clinical Manifestations ◽  
Rectal Mucosa ◽  
Wild Type ◽  
E Coli ◽  
Healthy Cattle ◽  
Life Threatening ◽  
K 12 ◽  
The Impact

ABSTRACT The human pathogen Escherichia coli O157:H7 causes hemorrhagic colitis and life-threatening sequelae and transiently colonizes healthy cattle at the terminal rectal mucosa. This study analyzed virulence factors important for the clinical manifestations of human E. coli O157:H7 infection for their contribution to the persistence of E. coli in cattle. The colonizing ability of E. coli O157:H7 was compared with those of nonpathogenic E. coli K-12 and isogenic deletion mutants missing Shiga toxin (Stx), the adhesin intimin, its receptor Tir, hemolysin, or the ∼92-kb pO157. Fully ruminant steers received a single rectal application of one E. coli strain so that effects of mucosal attachment and survival at the terminal rectum could be measured without the impact of bacterial passage through the entire gastrointestinal tract. Colonization was monitored by sensitive recto-anal junction mucosal swab culture. Nonpathogenic E. coli K-12 did not colonize as well as E. coli O157:H7 at the bovine terminal rectal mucosa. The E. coli O157:H7 best able to persist had intimin, Tir, and the pO157. Strains missing even one of these factors were recovered in lower numbers and were cleared faster than the wild type. In contrast, E. coli O157:H7 strains that were missing Stx or hemolysin colonized like the wild type. For these three strains, the number of bacteria increased between days 1 and 4 postapplication and then decreased slowly. In contrast, the numbers of noncolonizing strains (K-12, Δtir, and Δeae) decreased from the day of application. These patterns consistently predicted long-term colonization or clearance of the bacteria from the bovine terminal rectal mucosa.

Escherichia coli O157:H7: Epidemiology, Pathogenesis, and Methods for Detection in Food

1992 ◽  
Vol 55 (7) ◽  
pp. 555-565 ◽  
Author(s):  
NISHA V. PADHYE ◽  
MICHAEL P. DOYLE
Keyword(s):  
Escherichia Coli ◽  
Care Center ◽  
Watery Diarrhea ◽  
Clinical Samples ◽  
Escherichia Coli O157 ◽  
Intestinal Epithelial ◽  
Day Care Center ◽  
E Coli ◽  
Life Threatening ◽  
Uremic Syndrome

Escherichia coli O157:H7 is now recognized as an important human pathogen. Illnesses caused by E. coli O157:H7 infection can range from self-limited, watery diarrhea to life-threatening manifestations such as hemolytic uremic syndrome or thrombotic thrombocytopenic purpura. The mode of transmission is primarily through food; however, person-to-person transmission also has been identified in some day-care center and nursing home out-breaks. Studies to date indicate that cattle are an important reservoir of the organism. Although adhesion to intestinal epithelial cells and verotoxins are considered important virulence factors in the pathogenesis of the organism, more research is are necessary to determine the exact mechanism of pathogenicity. There is need for a rapid diagnostic test for the detection of E. coli O157:H7 in food and in clinical samples. Several useful research reagents have been developed for detecting E. coli O157:H7; however, they must be applied to a procedure that is specific, sensitive, rapid, easy to use, and commercially available so that microbiological laboratories can readily use them.

Breastfeeding and the Risk of Life-threatening Enterotoxigenic Escherichia coli Diarrhea in Bangladeshi Infants and Children

PEDIATRICS ◽  
1997 ◽  
Vol 100 (6) ◽  
pp. e2-e2 ◽  
Author(s):  
J. D. Clemens ◽  
M. R. Rao ◽  
J. Chakraborty ◽  
M. Yunus ◽  
M. Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Infants And Children ◽  
Enterotoxigenic Escherichia Coli ◽  
Life Threatening

scholarly journals Sab, a Novel Autotransporter of Locus of Enterocyte Effacement-Negative Shiga-Toxigenic Escherichia coli O113:H21, Contributes to Adherence and Biofilm Formation

2009 ◽  
Vol 77 (8) ◽  
pp. 3234-3243 ◽  
Author(s):  
Sylvia Herold ◽  
James C. Paton ◽  
Adrienne W. Paton
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Gastrointestinal Disease ◽  
Pcr Analysis ◽  
Systemic Complications ◽  
E Coli ◽  
Acid Protein ◽  
Life Threatening ◽  
Uremic Syndrome ◽  
Enterocyte Effacement

ABSTRACT Shiga-toxigenic Escherichia coli (STEC) strains cause serious gastrointestinal disease, which can lead to potentially life-threatening systemic complications such as hemolytic-uremic syndrome. Although the production of Shiga toxin has been considered to be the main virulence trait of STEC for many years, the capacity to colonize the host intestinal epithelium is a crucial step in pathogenesis. In this study, we have characterized a novel megaplasmid-encoded outer membrane protein in locus of enterocyte effacement (LEE)-negative O113:H21 STEC strain 98NK2, termed Sab (for STEC autotransporter [AT] contributing to biofilm formation). The 4,296-bp sab gene encodes a 1,431-amino-acid protein with the features of members of the AT protein family. When expressed in E. coli JM109, Sab contributed to the diffuse adherence to human epithelial (HEp-2) cells and promoted biofilm formation on polystyrene surfaces. A 98NK2 sab deletion mutant was also defective in biofilm formation relative to its otherwise isogenic wild-type parent, and this was complemented by transformation with a sab-carrying plasmid. Interestingly, an unrelated O113:H21 STEC isolate that had a naturally occurring deletion in sab was similarly defective in biofilm formation. PCR analysis indicated that sab is present in LEE-negative STEC strains belonging to serotypes/groups O113:H21, O23, and O82:H8. These findings raise the possibility that Sab may contribute to colonization in a subset of LEE-negative STEC strains.

scholarly journals Isolasi dan uji kepekaan Escherichia coli O157:H7 Isolat lokal asal feses dapi terhadap berbagai jenis antibiotika

2018 ◽  
Vol 29 (2) ◽  
pp. 57
Author(s):  
I Wayan Suardana ◽  
I Nyoman Suarsana ◽  
Michael Haryadi Wibowo ◽  
Dyah Ayu Widiasih
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Life Threatening

Escherichia coli O157:H7 is one of the bacteria infection agents that life threatening.

scholarly journals Prevalence and molecular characterization of Shiga toxin-producing escherichia coli isolates from human and sheep in Al-Madinah Al-Munawarah

Infectio ◽  
2017 ◽  
Vol 21 (2) ◽  
Author(s):  
Eman Fathi Sharafa ◽  
Iman I. Shabanaa
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Shiga Toxin ◽  
Genetic Relatedness ◽  
Control Measures ◽  
Health Concern ◽  
Global Public Health ◽  
E Coli ◽  
Life Threatening

Shiga toxin-producing Escherichia coli (STEC) strains have emerged as important foodborne pathogens of global public health concern, causing life-threatening diseases. Sheep and their products have been documented as important reservoirs for STECs, especially E. coli O157. The aim of this study was to investigate STECs from diarrheal human and sheep in Al-Madinah Al-Munawarah, Saudi Arabia. Fecal samples were collected between June and August, 2015 from diarrheal humans (n = 134) and sheep (n = 87). Presumptive E. coli human-and sheep-isolated strains were identified for their serotypes, the associated virulence genes (Shiga toxin [stx1 , stx2 ], haemolysin [ehxA] and intimin [eae]) by polymerase chain reaction and their susceptibility to antibiotics. Pulsed-field gel electrophoresis (PFGE) was used to demonstrate the genetic relatedness between Serotype O157:H7 human- and sheep-isolated strains. Forty eight (48/221; 21.7%) STECs were recovered from both human and sheep, their serotypes were as follows: O157:H7, O26:H11, O157:HNM, O26:HNM, O128:H2, O48:HNM, O111:HNM and OUT:HUT. Various virulence profiles and multiple antibiotic resistance were observed among the isolates. Twenty eight O157:H7 serotypes (17 human isolates and 11 sheep isolates) were identified in 13 PFGE pulsotypes, where human and sheep isolates were highly related. PFGE banding profiles together with serotypes and genotypes afford proof that human and sheep can be colonized and infected with similar E. coli O157:H7 strains. Our findings highlight the importance of epidemiological and microbiological surveillance of STECs; as well as the development of control measures to decrease risks associated with zoonotic O157:H7.

scholarly journals Antimicrobial Susceptibility Pattern of Uropathogenic Bacteria in RMMC Hospital of Chidambaram

2019 ◽  
Vol 9 (4-A) ◽  
pp. 334-339
Author(s):  
A. Gnanasekaran ◽  
P. Manikandan ◽  
J. Vigneshwari ◽  
P. Poongothai ◽  
P. K. Senthilkumar
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Urine Samples ◽  
Resistance Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Antibiotic Susceptibility Test ◽  
Life Threatening ◽  
Uropathogenic Bacteria

Background: In every year millions of people were affected by the Urinary Tract Infection. It was creating a serious health issue. Aim: The present study was to analysis of the uropathogenic bacteria in patients were attended RMMC Hospital and their antibiotic resistance pattern, in vitro detection of haemolysis virulent factor of uropathogenic. Material and Methods: All urine samples were tested by the standard microbiological procedure. Kirby-Bauer method used for the Antibiotic Susceptibility Test according to the CLSI guidelines. Commercially available antibiotics were used. Blood Agar used for the detection of haemolysis. Results: A total of 261 urine samples were included in this study. We isolated a total of 103 positive cultures. 12% of Gram-positive, 83% of Gram-negative bacteria and 3% of Candida fungi. Escherichia coli was the most predominant bacteria (54%) followed by Klebsiella sp (15%), Staphylococcus aureus (12%), Pseudomonas aeruginosa (12%), Proteus (1%) and fungi Candida (3%). Mostly female patients’ sample were analysed and the inpatient higher majority than the outpatients. Conclusion: Escherichia coli are the common bacteria to cause of UTI. Nowadays most of the uropathogens are to resistance to the overall antibiotics. This kind of reactions creating the life-threatening of humans. Keywords: Antibiotic, Antibiotic Susceptibility Test, Uropathogens, Resistance, Haemolysis

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