Identification of Staphylococcus aureus from enriched nasal swabs within 24 h is improved with use of multiple culture media

2013 ◽  
Vol 62 (9) ◽  
pp. 1365-1367 ◽  
Author(s):  
Maya Nadimpalli ◽  
Christopher Heaney ◽  
Jill R. Stewart
Keyword(s):  
Staphylococcus Aureus ◽  
Culture Media ◽  
Nasal Carriage ◽  
Healthy Population ◽  
Staph Aureus ◽  
Nasal Swabs

Nasal carriage of Staphylococcus aureus is commonly evaluated via culture-based methods. We found that parallel use of two media, Baird-Parker and CHROMagar™ Staph aureus, increased detection of S. aureus from a healthy population by 29 %. We suggest use of both media for optimal identification of S. aureus from healthy cohorts.

Assessment of the Multifactorial Effect on Antimicrobial Sensitivity in Positive Staphylococcus Aureus Clinical Isolates from Assir Region, Saudi Arabia

2012 ◽  
Vol 13 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Nazar M Abdalla ◽  
Waleed O Haimour ◽  
Amani A Osman ◽  
Hassan Abdul Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Saudi Arabia ◽  
Culture Media ◽  
Meca Gene ◽  
Laboratory Data ◽  
Age Groups ◽  
Clinical Isolates ◽  
Diabetic Patients ◽  
Staph Aureus ◽  
Antimicrobial Sensitivity

General objectives: This study aimed at assessment of factors affecting antimicrobial sensitivity in Staphylococcus aureus clinical isolates from Assir region, Saudi Arabia. Materials and Methods: In this study, eighty one patients presented with Staph. aureus infections either nosocomial or community acquired infections were involved by collecting nasal swabs from them at Aseer Central Hospital General Lab. These patients were from all age groups and from males and females during the period of Jan 2011- Jun 2011. These samples were undergone variable laboratory procedures mainly; bactech, culture media, antibiotics sensitivity test using diffusion disc test (MIC) and molecular (PCR) for detection of mec A gene. Clinical and laboratory data were recorded in special formats and analyzed by statistical computer program (SPSS). Results: Showed that; Descriptive and analytical statistical analysis were performed and final results were plotted in tables. In Staph aureus MecA gene positive cases (50) showed: Oxacillin/ Mithicillin, Ciprofloxacin and Fusidin resistant in diabetic patients were 13, 26.0%, 9, 18% and 7, 14% respectively and in non diabetic patients were 37, 74.0%, 22, 44% and 20, 40% respectively. While no sensitivity in diabetic and non diabetic patients using Oxacillin/ Mithicillin. In Staph aureus MecA gene negative cases (31) showed: Oxacillin/ Mithicillin, sensitivity in diabetic patients (5, 16.1%) and in non diabetic were (26, 83.9%). While no resistant in diabetic and non diabetic patients. In Ciprofloxacin and Fusidin resistant in diabetic patients were 1, 3.2% and 1, 3.2% respectively and in non diabetic patients were 12, 38.7% and 7, 22.6%respectively. Erythromycin in Staph aureus ( MecA gene) positive cases (50) showed: resistant in age (0-15) years were (5, 10%), (16-50) years were (16, 32%) and ( ›50 years) were (12, 24%). Erythromycin in Staph aureus (MecA gene) negative cases (31) showed: resistant in age (0-15) years were (6, 19.3%), (16-50) years were (5, 16.1%) and ( ›50 years) were (3, 9.7%). Conclusion: Drugs resistance is a major progressive multifactorial problem facing the treatment of Staph aureus infections. DOI: http://dx.doi.org/10.3329/jom.v13i2.12750 J Medicine 2012; 13 : 152-159

scholarly journals Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Katerina Neradova ◽  
Vladislav Jakubu ◽  
Katarina Pomorska ◽  
Helena Zemlickova
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Nasal Carriage ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Nasal Swabs ◽  
Mrsa Strains ◽  
Healthcare Associated ◽  
Mrsa Colonization ◽  
Animal Strains

Abstract Background Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n = 5), ST398-t2330-IV (n = 1), ST398-t034-V (n = 1), ST225-t003-II (n = 1) and ST4894-t011-IV (n = 1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex spa-CC11. Conclusion According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare - associated MRSA strains are still not spread among animals.

A cross sectional study to assess nasal carriage of methicillin resistant Staphylococcus aureus in healthcare professionals in a tertiary care hospital

2020 ◽  
pp. 1-12
Author(s):  
Fibhaa Syed ◽  
Nasim Akhtar ◽  
Mohammad Ali Arif ◽  
Adil Ramzan ◽  
Rauf Niazi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Healthcare Workers ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tertiary Care ◽  
Nasal Carriage ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Nasal Swabs

Abstract Objective: To determine the nasal carriage of staphylococcus aureus and methicillin-resistant staphylococcus aureus among healthcare workers in a tertiary care setting. Methods: The cross-sectional study was conducted at the Pakistan Institute of Medical Sciences, Islamabad, Pakistan, from April to July 2018, and comprised healthcare workers at the institution. Nasal swabs were collected and cultured on Mannitol salt agar. Mannitol fermenting colonies which were gram-positive cocci, catalase-positive and coagulase-positive were identified as staphylococcus aureus. Antibiotic susceptibility test was performed by modified Kirby-Bauer disc diffusion method. Methicillin resistance was detected using cefoxitin disc diffusion method. Data was analysed using SPSS 23. Results: Of the 210 nasal swabs, 52(24.76%) had a staphylococcus aureus growth, and, of them, 15(7.1%) were methicillin-resistant. No association could be established with either any single category of healthcare worker or an inter-department variation (p>0.05). Likewise, there was no association with age, gender, duration of service, smoking, co-morbidities, use of antibiotics in the preceding six months, treating a patient with methicillin-resistant staphylococcus aureus in the preceding six months and hospitalisation in the preceding year (p>0.05). Conclusion: The frequency of nasal carriage of methicillin resistant staphylococcus aureus amongst healthcare workers was regardless of the nature of their professional engagement. Key Words: Methicillin resistant staphylococcus aureus, Nasal carriage, Continuous...

scholarly journals Clinical and Bacteriological Aspects of Impetigo Contagiosa

1955 ◽  
Vol 53 (4) ◽  
pp. 495-508 ◽  
Author(s):  
George I. Barrow
Keyword(s):  
Staphylococcus Aureus ◽  
Special Reference ◽  
Streptococcus Pyogenes ◽  
Phage Type ◽  
Nasal Carriage ◽  
Control Groups ◽  
Staph Aureus ◽  
Impetigo Contagiosa ◽  
Resistant Strains

Summary1. The results of an investigation into the clinical, epidemiological and bacteriological features of impetigo contagiosa, with special reference to the type identification of staphylococci and streptococci, are reported and discussed.2. Of 106 impetigo cases studied, Staphylococcus aureus was isolated alone from 86 lesions (81 %), Streptococcus pyogenes alone from 6 (5·6 %), and a mixed growth of Staph. aureus and haemolytic streptococci in 14 instances (13·2 %).3. Of the 100 strains of Staph. aureus isolated from impetigo lesions, 63 were identical in phage type (‘type 71’), and a further 17 were closely related (‘weak 71’).4. Only one representative of ‘type 71’, and 9 of ‘weak 71’, were obtained from 164 strains of Staph. aureus from 200 persons in three control groups.5. Of 90 strains of Staph. aureus from impetigo lesions, 64 (71 %) were resistant to penicillin. Of these penicillin-resistant strains, 54 (84 %) were of ‘type 71’, or close variants.6. Strep, pyogenes was probably causative in at least 6 of the 18 patients yielding this organism from lesions; it was presumed to be a secondary invader in the remainder.7. It is doubtful if nasal carriage is of importance in the epidemiology of impetigo.8. It is concluded that there is a specific ‘type’ of staphylococcus associated with this form of impetigo.

Nasal Carriage of Staphylococcus aureus in Healthy Adults and in School Children

2013 ◽  
Vol 30 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Marina Dinić ◽  
Slavica Vuković ◽  
Dobrila Stanković Đorđević ◽  
Milena Bogdanović
Keyword(s):  
Staphylococcus Aureus ◽  
School Children ◽  
Local Community ◽  
Nasal Carriage ◽  
Diffusion Method ◽  
Healthy Individuals ◽  
Disk Diffusion Method ◽  
Nasal Swabs ◽  
Mueller Hinton Agar ◽  
Therapeutic Problem

Summary Staphylococcus aureus (S. aureus) is a microorganism that colonizes the skin and mucosal surfaces of healthy individuals, but it is also one of the most common causes of community-acquired and hospital infections. Nasal carriage of S. aureus represents a major risk factor for the development of infection with this bacterium. A special therapeutic problem are methicillin-resistant isolates of S. aureus (MRSA). The aim of this study was to assess the nasal carriage of S. aureus in healthy individuals in the local community, and the sensitivity of the microorganism to antibiotics. The study enrolled 56.868 healthy individuals aged 19 to 65 years, and 2.040 healthy school children aged 15 to 19 years. Specimens to be studied were obtained from anterior nares. We used the disk diffusion method (Kirby-Bauer) on Mueller- Hinton agar to assess the sensitivity of isolated S. aureus. S. aureus was isolated in 1.381 (2.34%) respondents. Positive findings were obtained in 2.33% of adult examinees, and in 2.59% of studied school children. We found a low level of susceptibility only to penicillin (5.36%). The susceptibility of S. aureus isolates to all other tested antibiotics was present in a high percentage, with the lowest percentage of susceptibility to doxycycline (71.54%) and erythromycin (86.09%). The highest percentage of susceptibility of tested isolates was reported for fusidic acid (99.27%). In relation to the total number of S. aureus isolates from nasal swabs in adults, MRSA was present in 8.96% (119 isolates), while there were 4 MRSA isolates from nasal swabs in school children. In this study, we established a low percentage of nasal carriage of S. aureus in the population of healthy individuals, but a high percentage of MRSA.

scholarly journals Nasal Carriage and Methicillin Resistance of Staphylococcus Aureus Among Schoolchildren in Sana’a City, Yemen

2020 ◽  
Author(s):  
Arwa Mohammed Othman ◽  
Belques Sharaf Al-Huraibi ◽  
Rowa Mohammed Assayaghi ◽  
Huda Zaid Al-Shami
Keyword(s):  
Staphylococcus Aureus ◽  
Hospital Admission ◽  
Methicillin Resistance ◽  
Positive Culture ◽  
Nasal Carriage ◽  
Cross Sectional Study ◽  
High Morbidity ◽  
Carriage Rate ◽  
Cross Sectional ◽  
Nasal Swabs

Abstract Background: Staphylococcus aureus (S. aureus) is a frequent cause of serious health problems with high morbidity and mortality. The risk of S. aureus infections is increased with the emergence of methicillin-resistant S. aureus (MRSA). The aim of this study is to determine the nasal carriage rate of both S. aureus and MRSA among schoolchildren in Sana’a city.Methods: This is a cross-sectional study conducted from January 2018 to May 2020. Five hundred and eighty eight students were enrolled. Nasal swabs were collected from each student for culturing and methicillin susceptibility testing. Results: Out 588 nasal swab, 536 yielded bacterial growth. Students with positive culture were 271(51%) males and 265(49%) females. Their age ranged from 5 to 19 years old with mean age and standard deviation equaled to 13.3±3.5 years. S. aureus was isolated from 129 (24%) students whereas the overall prevalence of MRSA was 8(1.5%). S. aureus was significantly recovered from students at age group 10-14 years (χ2 = 7.02, p = 0.03), females than males (OR= 1.96, χ2 = 10.75, p = 0.001), and students who were admitted into hospitals (OR= 1.6, χ2 = 4.89, p = 0.03). Nevertheless, there were no significant differences between MRSA carriage and students’ age (χ2 = 2.3, p = 0.32), gender (OR= 1.02, χ2 = 0.001, p = 0.63), and hospital admission (OR= 1.4, χ2 = 0.25, p = 0.62). Conclusions: The prevalence of MRSA is low among schoolchildren in Sana’a city. Age, gender and previous hospital admission were statistically associated with nasal carriage of S. aureus but not MRSA nasal carriage.

scholarly journals Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

2019 ◽  
Author(s):  
Katerina Neradova ◽  
Vladislav Jakubu ◽  
Katarina Pomorska ◽  
Helena Zemlickova
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Nasal Carriage ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Nasal Swabs ◽  
Mrsa Strains ◽  
Healthcare Associated ◽  
Mrsa Colonization

Abstract Background Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n=5), ST398-t2330-IV (n=1), ST398-t034-V (n=1), ST225-t003-II (n=1) and ST4894-t011-IV (n=1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex CC11. Conclusion According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare - associated MRSA strains are still not spread among animals.

NASAL CARRIAGE OF STAPHYLOCOCCUS AUREUS AMONG HEALTHY POPULATION IN DIYALA

2007 ◽  
Vol 10 (2) ◽  
pp. 77-80
Author(s):  
Abdul-Razak S. Hasan ◽  
◽  
Abbas Aboud Al-Duliami ◽  
Suhair Abdul-Jabbar ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nasal Carriage ◽  
Healthy Population

scholarly journals Hospitalization and colonization by methicillin-resistant Staphylococcus aureus in the surgical department of 03 health facilities in the Ndé division, West-Cameroon

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
William Lelorel Nguekap Nankam ◽  
Pierre René Fotsing Kwetche ◽  
Gildas Boris Tazemda-Kuitsouc ◽  
Golda Joyce Djeutsa Chouna ◽  
Jean Michel Tekam
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Nasal Carriage ◽  
Health Facilities ◽  
Methicillin Resistant ◽  
Surgical Department ◽  
Commensal Flora ◽  
Nasal Swabs ◽  
Susceptibility Tests ◽  
The Impact

Abstract Background Commensal flora colonization during hospitalization by bacteria is the first step for nosocomial infections while antibiotic resistance reduces therapeutic options. In aim to control this phenomenon, we initiated this study to describe the impact of hospitalization on colonization by methicillin-resistant Staphylococcus aureus in the surgical department of 03 health facilities in the Ndé division, West-Cameroon. Methods This study was carried out on patients admitted for surgery in 03 health facilities of the Ndé division, West-Cameroon (District Hospital of Bangangté, Protestant Hospital of Bangwa and Cliniques Universitaires des Montagnes). After obtaining ethical clearance and authorizations, nasal swabs were performed at admission and discharge, with the aim of isolating bacteria and performing their antibiotic susceptibility tests. Informations on each participant's antibiotic therapy were recorded. Laboratory investigations were carried out according to standard protocols (CASFM, 2019). Results The most commonly used antibiotics were β-lactams. A total of 104 nasal swabs were performed on 52 patients who agreed to participate to the study. From the analysis, 110 (57 at admission versus 53 at discharge) Staphylococcus isolates were obtained. Overall, susceptibility testing showed that antibiotic resistance rates were higher at discharge than at admission; with significant differences between the susceptibility profiles obtained at admission and discharge for β-lactams and not significant for fluoroquinolones and aminoglycosides. Globally, frequency of nasal carriage of methicillin-resistant Staphylococcus aureus at discharge 16 (30.77%) was significantly higher than at admission 07 (13.46%) with Chi-2 = 4.52 and p = 0.0335. Conclusion The high rates of antibiotic resistance of bacteria isolated at discharge compared to those isolated at admission obtained in the present investigation, highlights the important role that hospitalization plays in the selection and dissemination of methicillin-resistant Staphylococcus aureus and colonization by these bacteria in health structures of Ndé division. As a result, further investigations to find the factors that promote this phenomenon should be carried out.

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