Campylobacter concisus and exotoxin 9 levels in paediatric patients with Crohn’s disease and their association with the intestinal microbiota

2014 ◽  
Vol 63 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Nadeem O. Kaakoush ◽  
Natalia Castaño-Rodríguez ◽  
Andrew S. Day ◽  
Daniel A. Lemberg ◽  
Steven T. Leach ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Intestinal Microbiota ◽  
Host Cells ◽  
Healthy Controls ◽  
Virulence Determinants ◽  
Pathogenic Potential ◽  
Campylobacter Concisus ◽  
Faecal Samples ◽  
Intestinal Microbial Flora

There is mounting evidence for a possible role for Campylobacter concisus in Crohn’s disease (CD). However, the pathogenic potential of C. concisus remains disputed due to its presence in healthy subjects. It is documented that genetic diversity exists within this species, with some strains possessing putative virulence determinants such as exotoxin 9/DnaI that may enable them to persist intracellularly in host cells. In order to clarify this, we employed real-time PCR to determine C. concisus and exotoxin 9 levels within faecal samples of CD patients and healthy controls, and correlated these levels with abundances of microbial taxa identified in a subset of subjects. Both C. concisus and exotoxin 9 levels were found to be higher in CD patients than healthy controls, suggesting not only that CD patients had a greater abundance of C. concisus but also that their strains were likely to be more virulent. Moreover, C. concisus levels correlated with the exotoxin 9 levels in CD patients but not in healthy controls, indicating that healthy controls were colonized by non-virulent C. concisus strains. Correlations with the intestinal microbiota found C. concisus levels to correlate with Eubacterium, Subdoligranulum and Blautia, while exotoxin 9 levels correlated with Dialister, Oscillospira, Lachnospira and Prevotella. This suggests that either the composition of the intestinal microbial flora has the ability to influence levels of both virulent and non-virulent C. concisus strains, or infection with C. concisus may modulate the levels of specific bacterial taxa within the gastrointestinal tract.

scholarly journals P020 MAM, an anti-inflammatory protein derived from Faecalibacterium prausnitzii as a biomarker in Crohn’s disease?

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S140-S141
Author(s):  
P McLellan ◽  
A Lavelle ◽  
L Brot ◽  
M Straube ◽  
L de Sordi ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Predictive Value ◽  
Healthy Controls ◽  
Relative Expression ◽  
Faecalibacterium Prausnitzii ◽  
Inflammatory Protein ◽  
Faecal Samples ◽  
Anti Inflammatory

Abstract Background The intestinal microbiota of patients with Crohn’s disease (CD) is characterised by a specific dysbiosis, including the loss of Faecalibacterium prausnitzii (F. prau), a major bacterium with anti-inflammatory properties. MAM protein (microbiotia anti-inflammatory molecule) produced by F. prau has recently been identified as partially carrying the anti-inflammatory activity of this bacterium. The description of microbiota for diagnostic purposes in CD is currently difficult to achieve. MAM could be a biomarker of dysbiosis. Our goal was to study the expression of MAM in the intestinal ecosystem and to evaluate its interest as a biomarker. Methods An in silico approach was used to determine primers of MAM gene sequences specific to two currently known phylogroups (I and II) of F. prau. Faecal samples were collected from a monocentric cohort of CD patients in flare (n = 24) and in remission (n = 24) and from healthy controls (n = 12). To measure MAM expression in vivo in the microbiota, we extracted total RNA and DNA from each sample and performed quantitative PCR (qPCR) with MAM primers (I and II), F. prau primers and universal bacterial primers. We compared the relative expression (ΔCt) of MAM mRNA and DNA with qPCR to the overall amount of bacteria in faecal samples. Results MAM analyses of F. prau phylogroup II were the most informative. We observed that the relative expression of MAM mRNA was statistically lower in active patients 5.84 × 10−5 [95% CI 3.8 × 10−6–2.1 × 10−3] and in remission 5.36 × 10−5 [95% CI 2.5 × 10−8–1.9 × 10−2] compared with healthy controls 5.42 × 10−4 [95% CI 8.5 × 10−5–9.1 × 10−2], (p = 0.004). There was no difference in ΔCt between flare and remission in CD patients. The most relevant relative expression threshold was 1.6 × 10−4. At this threshold, sensitivity was 81%, specificity 76%, positive predictive value 94% and negative predictive value 47%. In multivariate analysis, the relative expression of MAM was an independently associated with the diagnosis of CD; OR 2.961 [95% CI 2.026–4.831] (p = 0.003). Using relative expression of MAM DNA, we found a significant decrease in the flare group compared with remission patients and controls. We found a negative correlation of MAM DNA expression with CRP (p = 0.0053) and HBI (p = 0.025). Conclusion Our results showed that the loss of MAM RNA expression is observed in CD patients. Moreover, MAM DNA detection was associated with CD activity parameters. This opens up the prospect of an ecological biomarker of CD based on the expression of molecules from the intestinal ecosystem. These results will need to be validated in larger independent cohorts and their specificities compared with other pathological situations.

scholarly journals Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Laura Sanchis-Artero ◽  
Juan Francisco Martínez-Blanch ◽  
Sergio Manresa-Vera ◽  
Ernesto Cortés-Castell ◽  
Marina Valls-Gandia ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Intestinal Microbiota ◽  
Area Under The Curve ◽  
Alpha Diversity ◽  
Amplicon Sequencing ◽  
Fecal Microbiota ◽  
Multicenter Observational Study ◽  
Partial Restoration ◽  
Before And After

AbstractIntestinal dysbiosis is key in the onset and development of Crohn’s disease (CD). We evaluated the microbiota changes in CD patients before and after a six-month anti-TNF treatment, comparing these changes with the microbiota of healthy subjects. This prospective multicenter observational study involved 27 CD patients initiating anti-TNF treatment and 16 healthy individuals. Inflammatory activity was determined at baseline, 3 and 6 months, classifying patients into responders and non-responders. Fecal microbiota was analyzed by massive genomic sequencing thought 16S rRNA amplicon sequencing before and after six months of anti-TNF treatment. The CD cohort showed a decrease in genera of the class Clostridia, short-chain fatty acid producers, and an increase in the phylum Proteobacteria (p < 0.01) versus the healthy cohort. After anti-TNF treatment, the phylum Proteobacteria also increased in non-responders versus responders (13/27) (p < 0.005), with the class Clostridia increasing. In addition, alpha diversity increased in responders versus non-responders (p < 0.01), tending towards eubiosis. An association was found (p < 0.001) in the F.prausnitzii/E.coli ratio between responders and non-responders. The F/E ratio was the most accurate biomarker of anti-TNF response (area under the curve 0.87). Thus, anti-TNF treatment allows partial restoration of intestinal microbiota in responders and the F.prausnitzii/E.coli ratio can provide a reliable indicator of response to anti-TNF in CD.

Sa595 DIETARY INFLAMMATORY POTENTIAL AND FOOD PATTERNS IN RELATION TO GUT MICROBIOME AMONG CHILDREN WITH CROHN'S DISEASE: A COMPARATIVE STUDY WITH HEALTHY CONTROLS

2021 ◽  
Vol 160 (6) ◽  
pp. S-566
Author(s):  
Jessica Breton ◽  
Vincent Tu ◽  
Ceylan Tanes ◽  
Maire A. Conrad ◽  
Kelly Kachelries ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Comparative Study ◽  
Gut Microbiome ◽  
Healthy Controls ◽  
Food Patterns

Probing serum N-glycan patterns for rapid and precise detection of Crohn's disease

2021 ◽  
Author(s):  
Yonglei Wu ◽  
Yijie Chen ◽  
Haolin Chen ◽  
Chenjie Yang ◽  
Xizhong Shen ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Statistical Analysis ◽  
Crohn's Disease ◽  
Carbon Matrix ◽  
Healthy Controls

Serum N-glycan patterns from 50 Crohn‘s disease (CD) patients and 50 healthy controls were acquired by a carbon matrix-based platform. According to statistical analysis, eight specific N-glycans revealed remarkable performance for CD diagnosis.

scholarly journals Fecal microbiota Profiling in Irritable Bowel Syndrome and Inflammatory Bowel Disease Patients with Irritable Bowel Syndrome-Type Symptoms

2021 ◽  
Author(s):  
Xiufang Cui ◽  
Haiyang Wang ◽  
Ziping Ye ◽  
Yi Li ◽  
Xinyun Qiu ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Irritable Bowel Syndrome ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Intestinal Microbiota ◽  
Bowel Disease ◽  
Sectional Study ◽  
Genus Level ◽  
Inflammatory Bowel ◽  
Irritable Bowel

Abstract BACKGROUND: The intestinal microbiota is thought to be involved in the occurrence of Inflammatory Bowel Disease in remission (IBDR) with Irritable Bowel Syndrome (IBS)-type symptoms, but the specific distinct profile of these bacteria remains unclear. Therefore, the purpose of this research is to investigate this issue by conducting a cross-sectional study.METHODS: IBS patients were diagnosed according to Rome Ⅳ criteria, IBD diagnosed according to the criteria of European Crohn & Colitis Organization (ECCO), IBDR patients with IBS-type symptoms were defined according to related IBS-type symptoms meeting the Rome IV criteria in IBDR patients, and were included Crohn’s disease in remission (CDR) and ulcerative colitis in remission (UCR) based on Crohn’s Disease Activity Index (DAI) and Mayo Scoring System respectively. Healthy controls come from the physical examination center and exclude people with underlying diseases. All enrolled subjects were divided into six groups, as followed: Health Control, IBS, CDR with IBS-type symptoms (CDR-IBS+), CDR without IBS-type symptoms (CDR-IBS-), UCR-IBS+ and UCR-IBS-. We collected fresh fecal samples from all subjects and applied 16S rRNA sequencing analysis to detect the structure and diversity of the microbiota among different groups. RESULTS: A total of 97 subjects were included in this study, of which 18 were health controls, 34 IBS patients, 25 CDR and 20 UCR. The richness of intestinal microbiota in CDR-IBS-was significantly lower than that in the control and IBS groups based on the analysis of observed species and Chao index (P<0.05). The observed species index in CDR-IBS+ was significantly higher than CDR-IBS- group (median index: 254.8 vs 203, P=0.036). No difference was found in Alpha diversity between UCR-IBS+ and UCR-IBS-. At phylum level, there was no significant difference between UC or CD with IBS-type symptoms and those without related symptoms. At genus level, the number of Faecalibacterium in CDR-IBS+ increased significantly while Fusobacterium decreased compared with CDR-IBS-(mean relative abundance of Faecalibacterium: 20.35% vs 5.18%, P<0.05; Fusobacterium: 1.51% vs 5.2%, P<0.05). However, compared with UCR-IBS - group, the number of Faecalibacterium in UCR-IBS+ group decreased, while the number of Streptococcus increased, but there was no statistical difference in the genus structure. Regardless of the phylum or genus level, the abundance and composition of the microbiota of IBS patients were not distinct from those of healthy people.CONCLUSIONS: CD patients in remission with IBS-type symptoms may be related to the increase of Faecalibacterium and decrease of Fusobacterium. UC patients in remission with IBS-type symptoms cannot be explained by changes in the abundance and structure of intestinal microbiota from our across-sectional study.

Reactive hyperaemia index as a marker of endothelial dysfunction in children with Crohn's disease is significantly lower than healthy controls

2014 ◽  
Vol 103 (2) ◽  
pp. e55-e60 ◽  
Author(s):  
Jehlicka Petr ◽  
Huml Michal ◽  
Schwarz Jan ◽  
Trefil Ladislav ◽  
Kobr Jiri ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Endothelial Dysfunction ◽  
Crohn's Disease ◽  
Healthy Controls ◽  
Reactive Hyperaemia

Sa1900 BIOMARKERS RESPONSE TO ANTI-TNF TREATMENT IN CROHN'S DISEASE THROUGH THE INTESTINAL MICROBIOTA

2020 ◽  
Vol 158 (6) ◽  
pp. S-471
Author(s):  
Laura Sanchis ◽  
Sergio Manresa-Vera ◽  
Juan F. Martínez ◽  
Marina Valls Gandia ◽  
Marisa Iborra ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Intestinal Microbiota

scholarly journals Serum adipokines as non-invasive biomarkers in Crohn’s disease

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Lorena Ortega Moreno ◽  
Ancor Sanz-Garcia ◽  
Marina J. Fernández de la Fuente ◽  
Ricardo Arroyo Solera ◽  
Samuel Fernández-Tomé ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Area Under The Curve ◽  
Healthy Controls ◽  
High Concentration ◽  
Serum Samples ◽  
Receiver Operating Characteristic Curves ◽  
Non Invasive ◽  
Healthy Control

Abstract Adipose tissue secretes molecules that can promote activity in Crohn’s disease. We aimed to evaluate the role of serum adipokines as possible biomarkers in Crohn’s disease. Serum samples were obtained from 40 patients with endoscopically active or quiescent Crohn’s disease and 36 healthy controls. Serum leptin, ghrelin, resistin and adiponectin levels were analysed by Multiplex in a Luminex 200 system technology. Receiver Operating Characteristic curves were performed to evaluate the adipokines discriminatory capacity. A logistic regression adjusted by possible confounders (i.e. gender, age, BMI) was performed for those adipokines that showed an area under the curve > 0.7. No differences were found in age, gender or BMI among groups. Distribution for serum resistin was different among the three groups of study, and only this adipokine showed an area under the curve of 0.75 comparing actives patients and healthy control groups. Resistin median concentration was selected as a cut-off for a logistic regression analysis; odds ratio along its 95% confidence interval adjusted by gender, age, and BMI yielded a value of 5.46 (1.34–22.14) comparing actives patients and healthy controls. High concentration of serum resistin is probably associated to activity, being this association independent of gender, age or BMI.

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