Inferring proteome dynamics during yeast cell cycle using gene expression data
AbstractProtein levels are most relevant physiologically, but measuring them genome-wide remains a challenge. In contrast, mRNA levels are much easier and less expensive to measure globally. Therefore, RNA levels are typically used to infer the corresponding protein levels. The steady-state condition (assumption that protein levels remain constant) is typically used to calculate protein abundances, as it is mathematically very convenient, even though it is often clear that it is not satisfied for proteins of interest. Here, we propose a simple, yet very effective, method to estimate genome wide protein abundances, which does not require the assumption that protein levels remain constant, and thus allows us to also predict proteome dynamics. Instead, we assume that the system returns to the baseline at the end of experiments; such an assumption is satisfied in many time-course experiments and in all periodic conditions (e.g. cell cycle). The approach only requires availability of gene expression and protein half-life data. As proof-of-concept, we calculated the predicted proteome dynamics for the budding yeast proteome during the cell cycle, which can be conveniently browsed online. The approach was validated experimentally by verifying that the predicted protein concentration changes were consistent with measurements for all proteins tested. Additionally, if proteomic data are also available, our approach can be used to predict how half-lives change in response to posttranslational regulation. We illustrated this application of our method withde novoprediction of changes in the degradation rate of Clb2 in response to post-translational modifications. The predicted changes were consistent with earlier observations in the literature.
